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The map label for this gene is mnmG [H]

Identifier: 194332860

GI number: 194332860

Start: 8261

End: 10123

Strand: Reverse

Name: mnmG [H]

Synonym: Paes_0007

Alternate gene names: 194332860

Gene position: 10123-8261 (Counterclockwise)

Preceding gene: 194332868

Following gene: 194332859

Centisome position: 0.4

GC content: 47.72

Gene sequence:

>1863_bases
ATGTATGATATTATTGTTGCCGGCGCAGGACATGCCGGTTGCGAGGCAGTGCTGGCAGGAGCAAGAATGGGTCACAAATG
CCTGCTCATCACATCTGATCTGACAGCAATCGCACGAATGAGCTGCAACCCGGCAATTGGCGGCGTCGCAAAAGGGCAGA
TAACAAGAGAGATCGACGCCCTTGGAGGCGAAATGGCAAAGGCAATTGACTCGACAGGAATACAATTTCGCATGCTCAAC
AGAAGCAAAGGTCCAGCAATGCACTCCCCCAGAGCACAAGCTGACCGAAACGAGTATACTCAGTATATGCGCCGGGTTAT
TGAACGCGAACCAAATATCGATCTCCTGCAGGATACCGTTATTGACATCAGAGCAGACAACGGCACCTGCCGGGGCGCCA
TCATCAGTACAGGAAGAATAATCGAATGCAGATCTCTGATCCTGGCATGCGGAACATTCCTGAATGGACTGATCCATATC
GGAATGAGCCACTATCCGGGAGGAAGAACACTGGCAGAACCACCGGTAGAAAAACTGACAGAAAACCTTGCAAGCCTCGG
ATTCAAGCAAGGAAGATTGAAAACAGGGACACCGGCCAGAATTGACAAAAGAAGTGTCGATTATACAAAGGTTTCGGAAC
AGCCTGGCGATACAGAACCAGTGCACTTCTCATTTACAACAGCACCTGCAAGAAAAGAGCAACTCAGCTGCCACCTCACA
GCCACAACCGAGGAAACCCACAAGATCCTCGAAACAGGCTTTGACCGCTCACCACTCTTTTCAGGAAAAGTTCAAGGCGT
CGGCCCCAGATACTGTCCGTCGATTGAAGACAAAATATTTCGATTCAAGGAGAGAAAAAGCCATCACATCTTTCTCGAAC
CAGAGGGATTCGACACGAACGAAATGTATGTAAACGGCTTCTCGACATCACTACCCGAAGATATCCAGATCAAGGCAATG
CGCTCTATTCCGGGTCTCGAACAGGTCAAGATCATAAGACCGGGGTATGCGATAGAATATGACTATTTCTATCCATGGCA
GCTTCACCCCACAATGGAAACAAAAGTCATAGAAAACCTCTACTTCGCAGGGCAGATCAATGGCACGTCTGGCTATGAAG
AAGCAGCCGCACAAGGCTTAATGGCCGGAATAAACGCTGCGTTGAAAATTGATGGGAAAACACCGTTGACGCTCAAAAGA
TCCGAGGCCTATATTGGAGTTCTGATAGATGACCTGATCACGAAAGAAACAAATGAACCGTATCGGATGTTCACATCGGC
AGCGGAACACAGGATCATTCTGAGGCAGGATAATGCCGACAGACGACTTACATCGTACGGTCACGCGTGTGGGTTGCAGA
CCAGCGAAACAATGGAGCGTCTGCGCCAAAAAGAGGCCGCTATTCACGCAATACAGGAACTTCTGCGAGAGTTCAGAATA
ACACCGGATATCCTCAAAAAACTCAGTAAACAGAACACATCAGCACTGCCGGATGAAAGCATCCGATGCATCAACCTCCT
GAAACGTCCACATGTATCCCTGCAATCCATCAAGGATGCAAGCAGTGAATTTCAAGAACGCCTCAATGCCATCAGCGCTG
ATCAGGATGTTCATGAACAGATTGAAATCGATATTAAATATGAAGGCTATATCAAGCGTGAACAACTGATCGCAGAAAAG
ATCCTGCGCCTTGAAGACAATATCATACCTCGATCATTCGACTATATGAAGATAAAAGGACTATCAAGCGAAGGCAGAGA
AAAACTTATCCGTCATCAACCCTCGACTATCGGCCAGGCATCAAGAATACTTGGTGTTACGCCTGCTGATATTTCAGTTC
TTATGGTCAAGATAGGGCGGTAG

Upstream 100 bases:

>100_bases
AAATATATTCCAGCATAACCACCAGTAACTCAACGTATTTTTCCTGAACGATACTGCCTCAGGAAAAATGTTTCACGTGA
AACATCAAGGAAAGAAACTT

Downstream 100 bases:

>100_bases
GCATCTCAAATGTTTCACGTGAAACATTCAATAAAACCCCGGCACACAACAGCAGATCAAGCCACAAACATGACGTCAAC
CATCCTCGATACAATACAAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MYDIIVAGAGHAGCEAVLAGARMGHKCLLITSDLTAIARMSCNPAIGGVAKGQITREIDALGGEMAKAIDSTGIQFRMLN
RSKGPAMHSPRAQADRNEYTQYMRRVIEREPNIDLLQDTVIDIRADNGTCRGAIISTGRIIECRSLILACGTFLNGLIHI
GMSHYPGGRTLAEPPVEKLTENLASLGFKQGRLKTGTPARIDKRSVDYTKVSEQPGDTEPVHFSFTTAPARKEQLSCHLT
ATTEETHKILETGFDRSPLFSGKVQGVGPRYCPSIEDKIFRFKERKSHHIFLEPEGFDTNEMYVNGFSTSLPEDIQIKAM
RSIPGLEQVKIIRPGYAIEYDYFYPWQLHPTMETKVIENLYFAGQINGTSGYEEAAAQGLMAGINAALKIDGKTPLTLKR
SEAYIGVLIDDLITKETNEPYRMFTSAAEHRIILRQDNADRRLTSYGHACGLQTSETMERLRQKEAAIHAIQELLREFRI
TPDILKKLSKQNTSALPDESIRCINLLKRPHVSLQSIKDASSEFQERLNAISADQDVHEQIEIDIKYEGYIKREQLIAEK
ILRLEDNIIPRSFDYMKIKGLSSEGREKLIRHQPSTIGQASRILGVTPADISVLMVKIGR

Sequences:

>Translated_620_residues
MYDIIVAGAGHAGCEAVLAGARMGHKCLLITSDLTAIARMSCNPAIGGVAKGQITREIDALGGEMAKAIDSTGIQFRMLN
RSKGPAMHSPRAQADRNEYTQYMRRVIEREPNIDLLQDTVIDIRADNGTCRGAIISTGRIIECRSLILACGTFLNGLIHI
GMSHYPGGRTLAEPPVEKLTENLASLGFKQGRLKTGTPARIDKRSVDYTKVSEQPGDTEPVHFSFTTAPARKEQLSCHLT
ATTEETHKILETGFDRSPLFSGKVQGVGPRYCPSIEDKIFRFKERKSHHIFLEPEGFDTNEMYVNGFSTSLPEDIQIKAM
RSIPGLEQVKIIRPGYAIEYDYFYPWQLHPTMETKVIENLYFAGQINGTSGYEEAAAQGLMAGINAALKIDGKTPLTLKR
SEAYIGVLIDDLITKETNEPYRMFTSAAEHRIILRQDNADRRLTSYGHACGLQTSETMERLRQKEAAIHAIQELLREFRI
TPDILKKLSKQNTSALPDESIRCINLLKRPHVSLQSIKDASSEFQERLNAISADQDVHEQIEIDIKYEGYIKREQLIAEK
ILRLEDNIIPRSFDYMKIKGLSSEGREKLIRHQPSTIGQASRILGVTPADISVLMVKIGR
>Mature_620_residues
MYDIIVAGAGHAGCEAVLAGARMGHKCLLITSDLTAIARMSCNPAIGGVAKGQITREIDALGGEMAKAIDSTGIQFRMLN
RSKGPAMHSPRAQADRNEYTQYMRRVIEREPNIDLLQDTVIDIRADNGTCRGAIISTGRIIECRSLILACGTFLNGLIHI
GMSHYPGGRTLAEPPVEKLTENLASLGFKQGRLKTGTPARIDKRSVDYTKVSEQPGDTEPVHFSFTTAPARKEQLSCHLT
ATTEETHKILETGFDRSPLFSGKVQGVGPRYCPSIEDKIFRFKERKSHHIFLEPEGFDTNEMYVNGFSTSLPEDIQIKAM
RSIPGLEQVKIIRPGYAIEYDYFYPWQLHPTMETKVIENLYFAGQINGTSGYEEAAAQGLMAGINAALKIDGKTPLTLKR
SEAYIGVLIDDLITKETNEPYRMFTSAAEHRIILRQDNADRRLTSYGHACGLQTSETMERLRQKEAAIHAIQELLREFRI
TPDILKKLSKQNTSALPDESIRCINLLKRPHVSLQSIKDASSEFQERLNAISADQDVHEQIEIDIKYEGYIKREQLIAEK
ILRLEDNIIPRSFDYMKIKGLSSEGREKLIRHQPSTIGQASRILGVTPADISVLMVKIGR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=40.5063291139241, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI19882217, Length=657, Percent_Identity=38.9649923896499, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI183227703, Length=672, Percent_Identity=38.0952380952381, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=48.7096774193548, Blast_Score=596, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=36.4516129032258, Blast_Score=403, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321202, Length=626, Percent_Identity=43.6102236421725, Blast_Score=479, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24658174, Length=626, Percent_Identity=42.0127795527157, Blast_Score=450, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69187; Mature: 69187

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDIIVAGAGHAGCEAVLAGARMGHKCLLITSDLTAIARMSCNPAIGGVAKGQITREIDA
CCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCHHHHHHHH
LGGEMAKAIDSTGIQFRMLNRSKGPAMHSPRAQADRNEYTQYMRRVIEREPNIDLLQDTV
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCE
IDIRADNGTCRGAIISTGRIIECRSLILACGTFLNGLIHIGMSHYPGGRTLAEPPVEKLT
EEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
ENLASLGFKQGRLKTGTPARIDKRSVDYTKVSEQPGDTEPVHFSFTTAPARKEQLSCHLT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEE
ATTEETHKILETGFDRSPLFSGKVQGVGPRYCPSIEDKIFRFKERKSHHIFLEPEGFDTN
ECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
EMYVNGFSTSLPEDIQIKAMRSIPGLEQVKIIRPGYAIEYDYFYPWQLHPTMETKVIENL
CEEEECCCCCCCCCCEEHHHHCCCCCCCEEEECCCEEEEECEECCEEECCCHHHHHHHHE
YFAGQINGTSGYEEAAAQGLMAGINAALKIDGKTPLTLKRSEAYIGVLIDDLITKETNEP
EEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCEEEHHHHHHHHCCCCCH
YRMFTSAAEHRIILRQDNADRRLTSYGHACGLQTSETMERLRQKEAAIHAIQELLREFRI
HHHHHHHHHCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TPDILKKLSKQNTSALPDESIRCINLLKRPHVSLQSIKDASSEFQERLNAISADQDVHEQ
CHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IEIDIKYEGYIKREQLIAEKILRLEDNIIPRSFDYMKIKGLSSEGREKLIRHQPSTIGQA
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHCCH
SRILGVTPADISVLMVKIGR
HHEECCCCCCEEEEEEEECC
>Mature Secondary Structure
MYDIIVAGAGHAGCEAVLAGARMGHKCLLITSDLTAIARMSCNPAIGGVAKGQITREIDA
CCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCHHHHHHHH
LGGEMAKAIDSTGIQFRMLNRSKGPAMHSPRAQADRNEYTQYMRRVIEREPNIDLLQDTV
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCE
IDIRADNGTCRGAIISTGRIIECRSLILACGTFLNGLIHIGMSHYPGGRTLAEPPVEKLT
EEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
ENLASLGFKQGRLKTGTPARIDKRSVDYTKVSEQPGDTEPVHFSFTTAPARKEQLSCHLT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEE
ATTEETHKILETGFDRSPLFSGKVQGVGPRYCPSIEDKIFRFKERKSHHIFLEPEGFDTN
ECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
EMYVNGFSTSLPEDIQIKAMRSIPGLEQVKIIRPGYAIEYDYFYPWQLHPTMETKVIENL
CEEEECCCCCCCCCCEEHHHHCCCCCCCEEEECCCEEEEECEECCEEECCCHHHHHHHHE
YFAGQINGTSGYEEAAAQGLMAGINAALKIDGKTPLTLKRSEAYIGVLIDDLITKETNEP
EEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCEEEHHHHHHHHCCCCCH
YRMFTSAAEHRIILRQDNADRRLTSYGHACGLQTSETMERLRQKEAAIHAIQELLREFRI
HHHHHHHHHCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TPDILKKLSKQNTSALPDESIRCINLLKRPHVSLQSIKDASSEFQERLNAISADQDVHEQ
CHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IEIDIKYEGYIKREQLIAEKILRLEDNIIPRSFDYMKIKGLSSEGREKLIRHQPSTIGQA
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHCCH
SRILGVTPADISVLMVKIGR
HHEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA