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The map label for this gene is capI [H]

Identifier: 193213441

GI number: 193213441

Start: 1972828

End: 1973880

Strand: Direct

Name: capI [H]

Synonym: Cpar_1802

Alternate gene names: 193213441

Gene position: 1972828-1973880 (Clockwise)

Preceding gene: 193213422

Following gene: 193213442

Centisome position: 86.18

GC content: 58.12

Gene sequence:

>1053_bases
GTGAAAATACTTGTTACCGGCACGGCCGGATTCATCGGCTTCCACCTTGCCGAACGCCTTGCTCAGAGAGGCGACGAAGT
GGTCGGTCTCGACAACATCAACGACTATTACGACCAGAACGTCAAGTACGGACGGCTCGCATTCTCAGGCATAGACCGCG
ATGCCATCGAAGAGGGCAAACTCGTTACCTCGACAAAGTATCCGAACTACCGCTTTATCAAGCTCGATCTCGAAGACAAG
GCCGCAATTGACGCGCTGTTCGCCGCCGAGCAGTTCGACGCAGTCTGCAACCTTGCGGCACAGGCCGGGGTGCGCTACTC
GCTGACCAACCCCGACGCCTACATCAAGTCGAACATCACCGGCTTCATCAATCTGCTCGAAGCATGCCGCCACAACAAGG
TCGGCAACCTCAGCTACGCATCGAGCTCGTCGGTGTACGGCCTGAACGAACGCCAGCCTTTTTCGGTGCACCACAATGTC
GATCACCCGGTGAGCCTGTACGCGGCCAGCAAGAAGTCCAACGAGCTGATGGCGCACACCTACAGCCACCTGTTCGGCAT
CCCGACCACCGGCCTGCGCTTCTTCACGGTCTACGGGCCGTGGGGACGGCCAGACATGGCGCTGTTCCTGTTCACCAAAG
CCGCGCTCGAAGGCCGCCCGATCGACGTCTTCAACTACGGCAATATGCAGCGCGACTTCACCTACATCGATGACATCATC
GAAGGCGTGGTACGCGTTATCGACAATCCGGCCAAATCGAATCCAAACTGGAGTGGCCAGAACCCCGATCCCGGCACTTC
GTCGGCCCCGTACCGCGTTTACAACATCGGCAACAACGAGCCGGTCAGGCTGTTGGATTTCATCGAGGCCATTGAAAAAG
CGCTCGGCAAAACCATCGAAAAGAACATGCTGCCAATCCAGCCGGGCGACGTTCCCTCGACCTACGCCGACGTCACCGAC
CTGGTCGAAGAGCTTGGCTACAGACCTGCCACACCGGTTCAGGAGGGCATCAACCGCTTCGTTGCCTGGTACCGTGAATT
CTTCAACGTCTGA

Upstream 100 bases:

>100_bases
AACGAAAAACCTGCCACATGCCCCATCAGGAGTAATCGCAGGTATCCCGGCCGGATTCCCGTAATCGCCCGGCGAAACCG
GGCCTCATCCAGCATTTTTT

Downstream 100 bases:

>100_bases
GGCGCTGGAGATGGGCATCGACGGCCGACTGTAAACCATGAACATTCTCTTTATCAACTCGGCAAGAACCTGGGGAGGAA
CCGAGAAATGGACCTCCATG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKILVTGTAGFIGFHLAERLAQRGDEVVGLDNINDYYDQNVKYGRLAFSGIDRDAIEEGKLVTSTKYPNYRFIKLDLEDK
AAIDALFAAEQFDAVCNLAAQAGVRYSLTNPDAYIKSNITGFINLLEACRHNKVGNLSYASSSSVYGLNERQPFSVHHNV
DHPVSLYAASKKSNELMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDII
EGVVRVIDNPAKSNPNWSGQNPDPGTSSAPYRVYNIGNNEPVRLLDFIEAIEKALGKTIEKNMLPIQPGDVPSTYADVTD
LVEELGYRPATPVQEGINRFVAWYREFFNV

Sequences:

>Translated_350_residues
MKILVTGTAGFIGFHLAERLAQRGDEVVGLDNINDYYDQNVKYGRLAFSGIDRDAIEEGKLVTSTKYPNYRFIKLDLEDK
AAIDALFAAEQFDAVCNLAAQAGVRYSLTNPDAYIKSNITGFINLLEACRHNKVGNLSYASSSSVYGLNERQPFSVHHNV
DHPVSLYAASKKSNELMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDII
EGVVRVIDNPAKSNPNWSGQNPDPGTSSAPYRVYNIGNNEPVRLLDFIEAIEKALGKTIEKNMLPIQPGDVPSTYADVTD
LVEELGYRPATPVQEGINRFVAWYREFFNV
>Mature_350_residues
MKILVTGTAGFIGFHLAERLAQRGDEVVGLDNINDYYDQNVKYGRLAFSGIDRDAIEEGKLVTSTKYPNYRFIKLDLEDK
AAIDALFAAEQFDAVCNLAAQAGVRYSLTNPDAYIKSNITGFINLLEACRHNKVGNLSYASSSSVYGLNERQPFSVHHNV
DHPVSLYAASKKSNELMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDII
EGVVRVIDNPAKSNPNWSGQNPDPGTSSAPYRVYNIGNNEPVRLLDFIEAIEKALGKTIEKNMLPIQPGDVPSTYADVTD
LVEELGYRPATPVQEGINRFVAWYREFFNV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=354, Percent_Identity=27.1186440677966, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI7657641, Length=357, Percent_Identity=25.4901960784314, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=369, Percent_Identity=26.0162601626016, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI56118217, Length=369, Percent_Identity=26.0162601626016, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI189083684, Length=369, Percent_Identity=26.0162601626016, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788353, Length=370, Percent_Identity=26.7567567567568, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994969, Length=365, Percent_Identity=26.3013698630137, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786974, Length=362, Percent_Identity=24.8618784530387, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=362, Percent_Identity=26.5193370165746, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=366, Percent_Identity=26.2295081967213, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=351, Percent_Identity=26.2108262108262, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=354, Percent_Identity=24.0112994350282, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115532424, Length=286, Percent_Identity=21.6783216783217, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=370, Percent_Identity=22.972972972973, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=26.0504201680672, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356223, Length=350, Percent_Identity=24.8571428571429, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39106; Mature: 39106

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGTAGFIGFHLAERLAQRGDEVVGLDNINDYYDQNVKYGRLAFSGIDRDAIEEGK
CEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCCCEEEECCCCHHHHHCCC
LVTSTKYPNYRFIKLDLEDKAAIDALFAAEQFDAVCNLAAQAGVRYSLTNPDAYIKSNIT
EEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
GFINLLEACRHNKVGNLSYASSSSVYGLNERQPFSVHHNVDHPVSLYAASKKSNELMAHT
HHHHHHHHHHCCCCCCCEECCCCCEECCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHH
YSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDII
HHHHHCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHH
EGVVRVIDNPAKSNPNWSGQNPDPGTSSAPYRVYNIGNNEPVRLLDFIEAIEKALGKTIE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
KNMLPIQPGDVPSTYADVTDLVEELGYRPATPVQEGINRFVAWYREFFNV
HCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVTGTAGFIGFHLAERLAQRGDEVVGLDNINDYYDQNVKYGRLAFSGIDRDAIEEGK
CEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCCCEEEECCCCHHHHHCCC
LVTSTKYPNYRFIKLDLEDKAAIDALFAAEQFDAVCNLAAQAGVRYSLTNPDAYIKSNIT
EEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
GFINLLEACRHNKVGNLSYASSSSVYGLNERQPFSVHHNVDHPVSLYAASKKSNELMAHT
HHHHHHHHHHCCCCCCCEECCCCCEECCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHH
YSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDII
HHHHHCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHH
EGVVRVIDNPAKSNPNWSGQNPDPGTSSAPYRVYNIGNNEPVRLLDFIEAIEKALGKTIE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
KNMLPIQPGDVPSTYADVTDLVEELGYRPATPVQEGINRFVAWYREFFNV
HCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]