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The map label for this gene is punA [H]

Identifier: 193213297

GI number: 193213297

Start: 1807833

End: 1808654

Strand: Direct

Name: punA [H]

Synonym: Cpar_1655

Alternate gene names: 193213297

Gene position: 1807833-1808654 (Clockwise)

Preceding gene: 193213296

Following gene: 193213298

Centisome position: 78.97

GC content: 57.06

Gene sequence:

>822_bases
ATGACTGAACAAAGGCAGAAAATCCGGGAAGCCGTTGAATTCATCAGAAAAAAAACTACGGCTGAATATCCGGTAGGTAT
CGTGCTCGGAACCGGCCTTGGCGGACTGGTCAAGGAGATCGAAATCGATTTTTCGCTCGATTATGCCGACATTCCCTATT
TTCCGATTTCGACGGTTGAAACCCATCACGGCAAGCTGATTTTCGGCACCCTCGCTGGCAAGAAGGTCGTTGCCATGCAG
GGCCGTTTCCACTACTACGAAGGCTACTCGATGACGCAGATCGTCTTCCCGATCAGGGTCATGAAGCAGCTCGGCATGAA
GACCCTCGGCATCACCAACGCCTGCGGTGGCATGAACCCCGGCTACAGCAAAGGCGACATCATGCTCATCGACGACCATA
TCAACCTGCTCGGCGGCAACCCGCTGATCGGCCCGAACGATCCTGAAATGGGCCCCCGCTTCCCCGACATGTGCGCTCCC
TACTCGCCGAGAATCCTCGAAATCGCAGAGAAAGTGGCACTCGAAAATGGCATCAAGGTGCAGCGCGGTGTTTACGTCGC
CGTCTCTGGCCCGTGCCTCGAAACCCGCGCCGAGTACCGCATGCTCAGGACGCTGGGCGCTGACGTGGTCGGCATGTCCA
CCGTACCGGAAGTGATCGCCGCCGTGCACCAGGGCACCGAGGTGTTCGGCATGTCCATCGTCACCGACGAGTGCTTCCCG
GACTGCCTCGTTCCTGTCGGTATCGAAGAGATCATCGAAGTCTCCAACCGCGCCGAGCCGAACATGACCACCATTTTCAG
GAACGTCGTAGCCAATCTTTAA

Upstream 100 bases:

>100_bases
TTATCAACTCCTCCCCGCCTTTCATCTCTCCCCGTAGCTGCTTATATTTAATGTCTTTTTTGCATGCCGTTTTAAATCCA
TCAAAACCACAGGGAATATC

Downstream 100 bases:

>100_bases
TCCTTAACAAGGCAATACCTGTTCTCTCATGATAGACGCTATCTCATTTAAAAACGGCACTTTCCGTTACCTCGACCAGC
GCTTCCTGCCCCTGCAGGAG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTEQRQKIREAVEFIRKKTTAEYPVGIVLGTGLGGLVKEIEIDFSLDYADIPYFPISTVETHHGKLIFGTLAGKKVVAMQ
GRFHYYEGYSMTQIVFPIRVMKQLGMKTLGITNACGGMNPGYSKGDIMLIDDHINLLGGNPLIGPNDPEMGPRFPDMCAP
YSPRILEIAEKVALENGIKVQRGVYVAVSGPCLETRAEYRMLRTLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
DCLVPVGIEEIIEVSNRAEPNMTTIFRNVVANL

Sequences:

>Translated_273_residues
MTEQRQKIREAVEFIRKKTTAEYPVGIVLGTGLGGLVKEIEIDFSLDYADIPYFPISTVETHHGKLIFGTLAGKKVVAMQ
GRFHYYEGYSMTQIVFPIRVMKQLGMKTLGITNACGGMNPGYSKGDIMLIDDHINLLGGNPLIGPNDPEMGPRFPDMCAP
YSPRILEIAEKVALENGIKVQRGVYVAVSGPCLETRAEYRMLRTLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
DCLVPVGIEEIIEVSNRAEPNMTTIFRNVVANL
>Mature_272_residues
TEQRQKIREAVEFIRKKTTAEYPVGIVLGTGLGGLVKEIEIDFSLDYADIPYFPISTVETHHGKLIFGTLAGKKVVAMQG
RFHYYEGYSMTQIVFPIRVMKQLGMKTLGITNACGGMNPGYSKGDIMLIDDHINLLGGNPLIGPNDPEMGPRFPDMCAPY
SPRILEIAEKVALENGIKVQRGVYVAVSGPCLETRAEYRMLRTLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFPD
CLVPVGIEEIIEVSNRAEPNMTTIFRNVVANL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=275, Percent_Identity=43.6363636363636, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI47132622, Length=257, Percent_Identity=29.1828793774319, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1788746, Length=212, Percent_Identity=42.9245283018868, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17541190, Length=273, Percent_Identity=37.3626373626374, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323238, Length=262, Percent_Identity=42.3664122137405, Blast_Score=185, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24656093, Length=238, Percent_Identity=48.3193277310924, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45552887, Length=238, Percent_Identity=48.3193277310924, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24656090, Length=238, Percent_Identity=48.3193277310924, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI45552885, Length=238, Percent_Identity=48.3193277310924, Blast_Score=217, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24762376, Length=215, Percent_Identity=37.2093023255814, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29944; Mature: 29813

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQRQKIREAVEFIRKKTTAEYPVGIVLGTGLGGLVKEIEIDFSLDYADIPYFPISTVE
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHEEEEEEECCCCCCCCCCCCCC
THHGKLIFGTLAGKKVVAMQGRFHYYEGYSMTQIVFPIRVMKQLGMKTLGITNACGGMNP
CCCCEEEEEECCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
GYSKGDIMLIDDHINLLGGNPLIGPNDPEMGPRFPDMCAPYSPRILEIAEKVALENGIKV
CCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
QRGVYVAVSGPCLETRAEYRMLRTLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
ECCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEEEEEECHHCCC
DCLVPVGIEEIIEVSNRAEPNMTTIFRNVVANL
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TEQRQKIREAVEFIRKKTTAEYPVGIVLGTGLGGLVKEIEIDFSLDYADIPYFPISTVE
CHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHEEEEEEECCCCCCCCCCCCCC
THHGKLIFGTLAGKKVVAMQGRFHYYEGYSMTQIVFPIRVMKQLGMKTLGITNACGGMNP
CCCCEEEEEECCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
GYSKGDIMLIDDHINLLGGNPLIGPNDPEMGPRFPDMCAPYSPRILEIAEKVALENGIKV
CCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
QRGVYVAVSGPCLETRAEYRMLRTLGADVVGMSTVPEVIAAVHQGTEVFGMSIVTDECFP
ECCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEEEEEECHHCCC
DCLVPVGIEEIIEVSNRAEPNMTTIFRNVVANL
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]