Click here to switch to the map view.

The map label for this gene is nifK [H]

Identifier: 193213266

GI number: 193213266

Start: 1773523

End: 1774902

Strand: Direct

Name: nifK [H]

Synonym: Cpar_1623

Alternate gene names: 193213266

Gene position: 1773523-1774902 (Clockwise)

Preceding gene: 193213265

Following gene: 193213267

Centisome position: 77.47

GC content: 61.23

Gene sequence:

>1380_bases
ATGTTATTACGTCACACAAGCAAAGAGGTCAAAGAGCGCGAGGGGCTGACGATCAATCCGGCAAAGACCTGCCAGCCGAT
CGGCGCCATGTACGCTGCGCTCGGCATACACGGCTGCCTGCCCCACAGCCACGGCTCGCAGGGCTGCTGCTCCTACCACC
GCAGCACCCTGACCCGCCACTACAAAGAGCCCGTCATGGCCGCAACCAGTTCGTTCACCGAGGGCGCTTCGGTGTTCGGC
GGCCAGGCAAACCTGCTTTCGGCTATCGAGACCATCTTTTCGGTCTATGATCCGGAGGTGATCGCGGTGCACTCCACCTG
CCTGTCCGAAACCATCGGCGACGACTTGCAGCAGATCACCAAGAAGGCCAGCGACGACGGCAAGATTCCTGAAGGCAAAT
ACGTTATCTACGCCAGCACCCCGAGCTTCGTCGGCTCGCACGTCACCGGCTACGCCAACATGGTCGTCGGCATGACCGAG
CAGTTCGCCGTCTCCACCGGAGAGAAGAACGACAAGGTCAACATCATCGCCGGCTGGATGGAGCCTTCCGACATGAGGGA
GATCAAATCGCTCGCCTCGCGTCTCGGCGTCAAGATCGTGCTCTTCCCCGACACCTCCGACGTGCTCGACGCACCGCAGA
CCGGCAAGCACGAGTTCTACCCGAAAGGCGGCATCACCATCGACGAGCTGAAGACTGTTGGCGACAGCAAGTCCTCGATC
GCCGTGGGCTGCATCACCGCCGAGCCAGCAGCCGTCGCGCTCGACAAGAAGTGCAAGGTTCCGTTCGAGACGGTTGACAT
GCCGATCGGCCTGTCCGCCACCGACCGCTTCATCATGGCCCTCAGCAAGGCTGGCGGCGTCGAGGTTCCCGCAGAACTCA
CCGCCGAGCGTGGCCGCCTGGTGGACGTCATGGCCGACATGGAGCAGTACTTCTACGGCAAGAAAGTCGCGCTGTTCGGC
GACCCCGACCAGCTCATGCCGCTCACCGAGTTCCTGCTCGACCTCGGCATGGTGCCGAAGCACATCGTCAGCGGCACGCC
GGGTCTGCGGTTCGAGAAACGCATGAAGGAGATCCTTGAAAGAGCTCCGGGCGCGAACTTCAAGAATGGCCCGCAGGCTG
ACATGTTCCTGATGCACCAGTGGATCAAGAACGAGCCTGTCGATCTGCTCATCGGCAACACCTACGGCAAGTACATCGCA
CGTGACGAAGACATCCCGTTCGTGCGCTTCGGCTTCCCGATCCTCGACCGCATCGGCCACAGCTACTTCCCGAACATCGG
GTATGCCGGCTCGCTCAGGCTGGTCGAGAAAATCCTCGGCGTGCTCATGGATCAGCAGGATCGCACGGCGCTGGAAGAGA
AGTTCGAGTTGGTTATGTGA

Upstream 100 bases:

>100_bases
TGGAAGCCAGCTACGTCGCCTGAACAAGCACGGGCGTCCCTGAGATGCGCAAGAGGTTTTCAGAGACGCCCGCATAAAAA
AATTAACGAGGAGATTATTG

Downstream 100 bases:

>100_bases
TGCGCTGTCGGAGCCGGTTCGCGATCCAGAGGCTTGCCCGGACGGTGAAATCCAGAACACTGTCCGGCTCGTCCGCGAAC
CGCTCTCGACGACAACGTAT

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLLRHTSKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLSAIETIFSVYDPEVIAVHSTCLSETIGDDLQQITKKASDDGKIPEGKYVIYASTPSFVGSHVTGYANMVVGMTE
QFAVSTGEKNDKVNIIAGWMEPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITIDELKTVGDSKSSI
AVGCITAEPAAVALDKKCKVPFETVDMPIGLSATDRFIMALSKAGGVEVPAELTAERGRLVDVMADMEQYFYGKKVALFG
DPDQLMPLTEFLLDLGMVPKHIVSGTPGLRFEKRMKEILERAPGANFKNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFVRFGFPILDRIGHSYFPNIGYAGSLRLVEKILGVLMDQQDRTALEEKFELVM

Sequences:

>Translated_459_residues
MLLRHTSKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLSAIETIFSVYDPEVIAVHSTCLSETIGDDLQQITKKASDDGKIPEGKYVIYASTPSFVGSHVTGYANMVVGMTE
QFAVSTGEKNDKVNIIAGWMEPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITIDELKTVGDSKSSI
AVGCITAEPAAVALDKKCKVPFETVDMPIGLSATDRFIMALSKAGGVEVPAELTAERGRLVDVMADMEQYFYGKKVALFG
DPDQLMPLTEFLLDLGMVPKHIVSGTPGLRFEKRMKEILERAPGANFKNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFVRFGFPILDRIGHSYFPNIGYAGSLRLVEKILGVLMDQQDRTALEEKFELVM
>Mature_459_residues
MLLRHTSKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLSAIETIFSVYDPEVIAVHSTCLSETIGDDLQQITKKASDDGKIPEGKYVIYASTPSFVGSHVTGYANMVVGMTE
QFAVSTGEKNDKVNIIAGWMEPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITIDELKTVGDSKSSI
AVGCITAEPAAVALDKKCKVPFETVDMPIGLSATDRFIMALSKAGGVEVPAELTAERGRLVDVMADMEQYFYGKKVALFG
DPDQLMPLTEFLLDLGMVPKHIVSGTPGLRFEKRMKEILERAPGANFKNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFVRFGFPILDRIGHSYFPNIGYAGSLRLVEKILGVLMDQQDRTALEEKFELVM

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50241; Mature: 50241

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRHTSKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRH
CCCCCCHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
YKEPVMAATSSFTEGASVFGGQANLLSAIETIFSVYDPEVIAVHSTCLSETIGDDLQQIT
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
KKASDDGKIPEGKYVIYASTPSFVGSHVTGYANMVVGMTEQFAVSTGEKNDKVNIIAGWM
HHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEECC
EPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITIDELKTVGDSKSSI
CCHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCE
AVGCITAEPAAVALDKKCKVPFETVDMPIGLSATDRFIMALSKAGGVEVPAELTAERGRL
EEEEEECCCCCEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHH
VDVMADMEQYFYGKKVALFGDPDQLMPLTEFLLDLGMVPKHIVSGTPGLRFEKRMKEILE
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHH
RAPGANFKNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIARDEDIPFVRFGFPILDRIGH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCEEEECCHHHHHCCH
SYFPNIGYAGSLRLVEKILGVLMDQQDRTALEEKFELVM
HHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLLRHTSKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSTLTRH
CCCCCCHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
YKEPVMAATSSFTEGASVFGGQANLLSAIETIFSVYDPEVIAVHSTCLSETIGDDLQQIT
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
KKASDDGKIPEGKYVIYASTPSFVGSHVTGYANMVVGMTEQFAVSTGEKNDKVNIIAGWM
HHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEECC
EPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITIDELKTVGDSKSSI
CCHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCE
AVGCITAEPAAVALDKKCKVPFETVDMPIGLSATDRFIMALSKAGGVEVPAELTAERGRL
EEEEEECCCCCEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHH
VDVMADMEQYFYGKKVALFGDPDQLMPLTEFLLDLGMVPKHIVSGTPGLRFEKRMKEILE
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHH
RAPGANFKNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIARDEDIPFVRFGFPILDRIGH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCEEEECCHHHHHCCH
SYFPNIGYAGSLRLVEKILGVLMDQQDRTALEEKFELVM
HHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840948; 3473447; 8393705 [H]