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The map label for this gene is nifD [H]

Identifier: 193213265

GI number: 193213265

Start: 1771817

End: 1773445

Strand: Direct

Name: nifD [H]

Synonym: Cpar_1622

Alternate gene names: 193213265

Gene position: 1771817-1773445 (Clockwise)

Preceding gene: 193213264

Following gene: 193213266

Centisome position: 77.4

GC content: 56.72

Gene sequence:

>1629_bases
ATGCCAGCGAAAGTAAACTTACCAGATCCGGCCTCGATTACCGAGGAGCTGATCAATAAATACCCGGCCAAGGTCGCCAA
AAAGCGCAGGAAGTCGATCGTGGCCAACGACCCGGATACCATTCCCGAGGTACAGGCCAACGTCCGCACCGTGCCGGGCA
TCATCACGCAGCGCGGCTGCTCCTACGCAGGTTGTAAGGGTGTGGTTCTCGGACCGACGCGTGACATCGTCAACATCGTG
CACGGCCCGATCGGTTGCAGCTTCTACGCATGGCTGACCCGCCGCAACCAGACCAGACCGGAGACCCCGGAGCATGAAAA
CTACATCACCTACTGCTTCTCGACCGACATGCAGGAAGAGCACGTGGTGTTCGGTGGTGAAAAGAAACTGAAGGTTGCAA
TCCAGGAGGCTTATGACCTCTTCCACCCGAAGGCCATCGCGATCTTCTCGACCTGCCCGGTCGGCCTGATCGGTGATGAC
GTGCACGCCGTTTCGAGAGAGATGAAAGAGAAGCTCGGCGACTGCAACGTCTTCGGCTTCAGCTGCGAAGGCTACCGCGG
CGTCAGCCAGTCGGCTGGTCACCACATCGCCAACAACGGTGTGTTCAAGCACATGGTCGGCAACAACAACGAAATCAAGC
CCGGCAAGTTCAAAATCAACCTGCTCGGCGAGTACAACATCGGTGGCGACGCCTTCGAAATCGAGCGACTGCTTGAGAAG
AGCGGCATCACGCTGATTGCCTCTTTCAGCGGCAACTCGACGGTCGGAGCCATGGAAAACGCACACACCGCCGACCTCAA
CATCGTGCAGTGCCACCGCTCGATCAACTACATGGGCGACATGATGGAAACCAAGTACGGTATTCCATGGATGAAGGTCA
ACTTCGTCGGCGTCGAGTCCACTGCCAAGTCGCTGCGCAAGATCGCCGAGTACTTCGGTGATGCAGAGCTGAAAGCGAAG
GTCGAAGAGGTGATTGCCGAAGAGATGCCTGCCGTCAAGGAGATCATCGAAGAGATTCGCCCGCGCACCGAAGGCAAAAC
CGCCATGCTGTTCGTCGGTGGATCGCGTGCTCACCACTACCAGGATCTGTTCAACGAGCTTGGCATGACGACCCTCTCCG
CCGGTTACGAGTTCGCTCACCGCGATGACTACGAAGGTCGCGAAGTGCTGCCGAACATCAAGATCGACGCCGACAGCAAG
AACATCGAAGAGCTGAAGGTCACCGCCGATCCTGAGCTGTACAACCCGCGCAAGAGCCCGGAAGAGATCGAGGAGCTGAA
AGCCAAAGGCCTCGAAATGAACGGTTACACCGGCATGATGAAGCAGATGGTCCACAAGAGCCTGGTGATCGATGACGTCA
GCCACTACGAGTCTGAAAAGCTCATCGAGATCTACAAGCCCGATATCTTCTGCGCCGGTATCAAAAAGAAGTACGTCGTC
CAGAAGATGGGTGTACCGCTCAAGCAGCTCCACAGCTACGACTACGGCGGTCCTTACACCGGCTTCAAAGGTGCAGTCAA
CTTCTACAAGGATATCGACCGCATGGTCAACAACCCTGTCTGGAAGATGATCAAGGCCCCCTGGGAGAAGGCTGAATCGG
AATCGCTGGAAGCCAGCTACGTCGCCTGA

Upstream 100 bases:

>100_bases
CTCGATACCATCAGGGTCAGAACCGGTGAAGAAGGCGACGTGGCTCTGAACTGAAGCTGAACTGAGATAACTACTAAAGG
TGTAAACGGAGAACGCTACA

Downstream 100 bases:

>100_bases
ACAAGCACGGGCGTCCCTGAGATGCGCAAGAGGTTTTCAGAGACGCCCGCATAAAAAAATTAACGAGGAGATTATTGATG
TTATTACGTCACACAAGCAA

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MPAKVNLPDPASITEELINKYPAKVAKKRRKSIVANDPDTIPEVQANVRTVPGIITQRGCSYAGCKGVVLGPTRDIVNIV
HGPIGCSFYAWLTRRNQTRPETPEHENYITYCFSTDMQEEHVVFGGEKKLKVAIQEAYDLFHPKAIAIFSTCPVGLIGDD
VHAVSREMKEKLGDCNVFGFSCEGYRGVSQSAGHHIANNGVFKHMVGNNNEIKPGKFKINLLGEYNIGGDAFEIERLLEK
SGITLIASFSGNSTVGAMENAHTADLNIVQCHRSINYMGDMMETKYGIPWMKVNFVGVESTAKSLRKIAEYFGDAELKAK
VEEVIAEEMPAVKEIIEEIRPRTEGKTAMLFVGGSRAHHYQDLFNELGMTTLSAGYEFAHRDDYEGREVLPNIKIDADSK
NIEELKVTADPELYNPRKSPEEIEELKAKGLEMNGYTGMMKQMVHKSLVIDDVSHYESEKLIEIYKPDIFCAGIKKKYVV
QKMGVPLKQLHSYDYGGPYTGFKGAVNFYKDIDRMVNNPVWKMIKAPWEKAESESLEASYVA

Sequences:

>Translated_542_residues
MPAKVNLPDPASITEELINKYPAKVAKKRRKSIVANDPDTIPEVQANVRTVPGIITQRGCSYAGCKGVVLGPTRDIVNIV
HGPIGCSFYAWLTRRNQTRPETPEHENYITYCFSTDMQEEHVVFGGEKKLKVAIQEAYDLFHPKAIAIFSTCPVGLIGDD
VHAVSREMKEKLGDCNVFGFSCEGYRGVSQSAGHHIANNGVFKHMVGNNNEIKPGKFKINLLGEYNIGGDAFEIERLLEK
SGITLIASFSGNSTVGAMENAHTADLNIVQCHRSINYMGDMMETKYGIPWMKVNFVGVESTAKSLRKIAEYFGDAELKAK
VEEVIAEEMPAVKEIIEEIRPRTEGKTAMLFVGGSRAHHYQDLFNELGMTTLSAGYEFAHRDDYEGREVLPNIKIDADSK
NIEELKVTADPELYNPRKSPEEIEELKAKGLEMNGYTGMMKQMVHKSLVIDDVSHYESEKLIEIYKPDIFCAGIKKKYVV
QKMGVPLKQLHSYDYGGPYTGFKGAVNFYKDIDRMVNNPVWKMIKAPWEKAESESLEASYVA
>Mature_541_residues
PAKVNLPDPASITEELINKYPAKVAKKRRKSIVANDPDTIPEVQANVRTVPGIITQRGCSYAGCKGVVLGPTRDIVNIVH
GPIGCSFYAWLTRRNQTRPETPEHENYITYCFSTDMQEEHVVFGGEKKLKVAIQEAYDLFHPKAIAIFSTCPVGLIGDDV
HAVSREMKEKLGDCNVFGFSCEGYRGVSQSAGHHIANNGVFKHMVGNNNEIKPGKFKINLLGEYNIGGDAFEIERLLEKS
GITLIASFSGNSTVGAMENAHTADLNIVQCHRSINYMGDMMETKYGIPWMKVNFVGVESTAKSLRKIAEYFGDAELKAKV
EEVIAEEMPAVKEIIEEIRPRTEGKTAMLFVGGSRAHHYQDLFNELGMTTLSAGYEFAHRDDYEGREVLPNIKIDADSKN
IEELKVTADPELYNPRKSPEEIEELKAKGLEMNGYTGMMKQMVHKSLVIDDVSHYESEKLIEIYKPDIFCAGIKKKYVVQ
KMGVPLKQLHSYDYGGPYTGFKGAVNFYKDIDRMVNNPVWKMIKAPWEKAESESLEASYVA

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 60605; Mature: 60474

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKVNLPDPASITEELINKYPAKVAKKRRKSIVANDPDTIPEVQANVRTVPGIITQRGC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCHHHHCCC
SYAGCKGVVLGPTRDIVNIVHGPIGCSFYAWLTRRNQTRPETPEHENYITYCFSTDMQEE
CCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCC
HVVFGGEKKLKVAIQEAYDLFHPKAIAIFSTCPVGLIGDDVHAVSREMKEKLGDCNVFGF
CEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SCEGYRGVSQSAGHHIANNGVFKHMVGNNNEIKPGKFKINLLGEYNIGGDAFEIERLLEK
CCCCCCCCCHHCCCCHHCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
SGITLIASFSGNSTVGAMENAHTADLNIVQCHRSINYMGDMMETKYGIPWMKVNFVGVES
CCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHH
TAKSLRKIAEYFGDAELKAKVEEVIAEEMPAVKEIIEEIRPRTEGKTAMLFVGGSRAHHY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
QDLFNELGMTTLSAGYEFAHRDDYEGREVLPNIKIDADSKNIEELKVTADPELYNPRKSP
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCH
EEIEELKAKGLEMNGYTGMMKQMVHKSLVIDDVSHYESEKLIEIYKPDIFCAGIKKKYVV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEECCHHHHHH
QKMGVPLKQLHSYDYGGPYTGFKGAVNFYKDIDRMVNNPVWKMIKAPWEKAESESLEASY
HHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
VA
CC
>Mature Secondary Structure 
PAKVNLPDPASITEELINKYPAKVAKKRRKSIVANDPDTIPEVQANVRTVPGIITQRGC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCHHHHCCC
SYAGCKGVVLGPTRDIVNIVHGPIGCSFYAWLTRRNQTRPETPEHENYITYCFSTDMQEE
CCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCC
HVVFGGEKKLKVAIQEAYDLFHPKAIAIFSTCPVGLIGDDVHAVSREMKEKLGDCNVFGF
CEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SCEGYRGVSQSAGHHIANNGVFKHMVGNNNEIKPGKFKINLLGEYNIGGDAFEIERLLEK
CCCCCCCCCHHCCCCHHCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
SGITLIASFSGNSTVGAMENAHTADLNIVQCHRSINYMGDMMETKYGIPWMKVNFVGVES
CCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHH
TAKSLRKIAEYFGDAELKAKVEEVIAEEMPAVKEIIEEIRPRTEGKTAMLFVGGSRAHHY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
QDLFNELGMTTLSAGYEFAHRDDYEGREVLPNIKIDADSKNIEELKVTADPELYNPRKSP
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCH
EEIEELKAKGLEMNGYTGMMKQMVHKSLVIDDVSHYESEKLIEIYKPDIFCAGIKKKYVV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEECCHHHHHH
QKMGVPLKQLHSYDYGGPYTGFKGAVNFYKDIDRMVNNPVWKMIKAPWEKAESESLEASY
HHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
VA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3473447; 7026551; 3457003; 2840948; 8393705 [H]