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The map label for this gene is vnfA [H]

Identifier: 193213260

GI number: 193213260

Start: 1765133

End: 1766752

Strand: Direct

Name: vnfA [H]

Synonym: Cpar_1615

Alternate gene names: 193213260

Gene position: 1765133-1766752 (Clockwise)

Preceding gene: 193213259

Following gene: 193213262

Centisome position: 77.11

GC content: 56.85

Gene sequence:

>1620_bases
ATGCTCATAGCGCACGAACAGGAACAGAGCAGCATCAGCCTTCTTGCCGAAGTAAGCAGGACCGTCAACAACGAAGAGGA
CATCAGCAAGGTGCTCCGACTGGTACTGTTCATCCTGTCGGAACACATGAACATGCTCCGCGGCATGGTGACCATTCTCG
ACAAAAGCACCGGTGAAATGGTCATCAACGAATCGTTCGGACTGAGCGAGGAGCAGAGCCAGCTTGGACGCTACAAAATC
GGTGAAGGCATTATCGGGCAGGTGGTTGAAACCGGCAAACCCAATGTGGTACCCCGTCTCGACGAAGAGCCGCTGTTCCT
TGACCGCACCCAGTCGAGAGTCGAAGAGAACAAAGAGGAGCTTTGCTTCATCTGCGTACCAATCATGGTGGGCAGCGAGG
TCATCGGCACGCTGAGCGCCGACCGCAGGATTTCCGTTCCCACAGAAAATGGCAACAAGCGGCGCAAAAGCGATCGGATC
GATATTCTGCAATACTATGTCGATCTGCTCTCCATCATCGCGGCCATGATTGCCCAGGCGGTTCGCATCAAGCAGCTCGA
TAACGAGGAGAAGAGCACGGACGCGCCCGAACACCCGAGCCACCCCGAGCATTCGGGCTACCCGATTGCGGAAGGCGAAT
TCGTTGAAGACGTTCCCGACGCCGACCGCCCGGCCAACATCATCGGCAACACCAAGCCCATGATGAGCCTTTTCAGGATG
ATCGACAAGATCTCCAAGACCAACGCCACAGCCCTGATTCTCGGCGAGAGCGGCGTCGGCAAGGAGCTGGTTGCCAATGC
GATCCACTACAAAAGCATGCGCAACGGCAAGCCGTTCATCAAGTTCAACTGCGCGGCGCTGCCGGAAAGTATCGTCGAAA
GCGAGCTGTTCGGCCACGAGAAAGGGGCCTTCACCGGAGCCGCCTCACTGCGGCGCGGACGCTTCGAGCTGGCCGATACC
GGCACGATTTTTCTGGACGAAGTGGGCGAGCTGAGCCTCTCCACGCAGGCCAAGCTGCTGCGGATCATTCAGGAGAAGGA
GTTCGAACGGCTCGGCGGCGCCAAGACCGTCAAGACCGACGTGCGCATCATCGCGGCCACCAACAGGAACCTCGAAGAAC
AGATTCAGCAGGGATCGTTCCGCGAGGATTTATACTACCGGCTCAACATTTTTCCGATCACCGTGCCGCCGCTTCGCGAG
CGCAAAACCGACATTCTGCTGCTGGCAGACTACTTTGTGGAAAAATTCAACACGGCCAACCACAAAGGGATTCGGCGGAT
TTCAACCACGGCCATCGACATGCTGATGCGCTACCACTGGCCCGGCAACGTGCGCGAGTTGCAGAACTGCATCGAGCGCG
CGGTCATCCTGAGCGAAGACAACGTCATCCACGGCTACCACCTGCCGCCCTCGCTCCAGACCGCCGAATCAAGCGGTACG
CCCTACATCGGCGACCTGCAGCAGAAGCTCGATTCGATCGAAAAGGAGATGATAATCGAAGCACTCAAACGCACCAAAGG
CAACATGACCAAAGCCGCTGCTCAACTCGGCTTGTCGGACAGAATCATGGGATTGAGGATGAAGAAGTTCAATATCGACT
ACAAGAAATTCCGACCTTGA

Upstream 100 bases:

>100_bases
ACGCCGACCGCCTCAAACGCTCGCTCAGGACGGACGAAATCGATGCCATCCGCGAAGGCACACTCATCAAGCAAGCTTAA
CTATCGAATAACAAGTCTTC

Downstream 100 bases:

>100_bases
TGGTAAAGGTTGGACGGATTCTGGAATGATCAGAATCGGGATGAAACAGAGGACGCCATTCATTTGATTTACTCCACGCT
CATGCGGTGAATCAGGTGAA

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLIAHEQEQSSISLLAEVSRTVNNEEDISKVLRLVLFILSEHMNMLRGMVTILDKSTGEMVINESFGLSEEQSQLGRYKI
GEGIIGQVVETGKPNVVPRLDEEPLFLDRTQSRVEENKEELCFICVPIMVGSEVIGTLSADRRISVPTENGNKRRKSDRI
DILQYYVDLLSIIAAMIAQAVRIKQLDNEEKSTDAPEHPSHPEHSGYPIAEGEFVEDVPDADRPANIIGNTKPMMSLFRM
IDKISKTNATALILGESGVGKELVANAIHYKSMRNGKPFIKFNCAALPESIVESELFGHEKGAFTGAASLRRGRFELADT
GTIFLDEVGELSLSTQAKLLRIIQEKEFERLGGAKTVKTDVRIIAATNRNLEEQIQQGSFREDLYYRLNIFPITVPPLRE
RKTDILLLADYFVEKFNTANHKGIRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSGT
PYIGDLQQKLDSIEKEMIIEALKRTKGNMTKAAAQLGLSDRIMGLRMKKFNIDYKKFRP

Sequences:

>Translated_539_residues
MLIAHEQEQSSISLLAEVSRTVNNEEDISKVLRLVLFILSEHMNMLRGMVTILDKSTGEMVINESFGLSEEQSQLGRYKI
GEGIIGQVVETGKPNVVPRLDEEPLFLDRTQSRVEENKEELCFICVPIMVGSEVIGTLSADRRISVPTENGNKRRKSDRI
DILQYYVDLLSIIAAMIAQAVRIKQLDNEEKSTDAPEHPSHPEHSGYPIAEGEFVEDVPDADRPANIIGNTKPMMSLFRM
IDKISKTNATALILGESGVGKELVANAIHYKSMRNGKPFIKFNCAALPESIVESELFGHEKGAFTGAASLRRGRFELADT
GTIFLDEVGELSLSTQAKLLRIIQEKEFERLGGAKTVKTDVRIIAATNRNLEEQIQQGSFREDLYYRLNIFPITVPPLRE
RKTDILLLADYFVEKFNTANHKGIRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSGT
PYIGDLQQKLDSIEKEMIIEALKRTKGNMTKAAAQLGLSDRIMGLRMKKFNIDYKKFRP
>Mature_539_residues
MLIAHEQEQSSISLLAEVSRTVNNEEDISKVLRLVLFILSEHMNMLRGMVTILDKSTGEMVINESFGLSEEQSQLGRYKI
GEGIIGQVVETGKPNVVPRLDEEPLFLDRTQSRVEENKEELCFICVPIMVGSEVIGTLSADRRISVPTENGNKRRKSDRI
DILQYYVDLLSIIAAMIAQAVRIKQLDNEEKSTDAPEHPSHPEHSGYPIAEGEFVEDVPDADRPANIIGNTKPMMSLFRM
IDKISKTNATALILGESGVGKELVANAIHYKSMRNGKPFIKFNCAALPESIVESELFGHEKGAFTGAASLRRGRFELADT
GTIFLDEVGELSLSTQAKLLRIIQEKEFERLGGAKTVKTDVRIIAATNRNLEEQIQQGSFREDLYYRLNIFPITVPPLRE
RKTDILLLADYFVEKFNTANHKGIRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSGT
PYIGDLQQKLDSIEKEMIIEALKRTKGNMTKAAAQLGLSDRIMGLRMKKFNIDYKKFRP

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=315, Percent_Identity=49.5238095238095, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1790437, Length=319, Percent_Identity=46.7084639498433, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI87082117, Length=504, Percent_Identity=35.1190476190476, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789087, Length=311, Percent_Identity=48.2315112540193, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082152, Length=309, Percent_Identity=45.3074433656958, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1789233, Length=352, Percent_Identity=40.625, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=39.6396396396396, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788905, Length=291, Percent_Identity=42.2680412371134, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=37.962962962963, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1786524, Length=327, Percent_Identity=40.0611620795107, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=37.6947040498442, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1789828, Length=237, Percent_Identity=37.9746835443038, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87081858, Length=302, Percent_Identity=31.4569536423841, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60646; Mature: 60646

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAHEQEQSSISLLAEVSRTVNNEEDISKVLRLVLFILSEHMNMLRGMVTILDKSTGEM
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VINESFGLSEEQSQLGRYKIGEGIIGQVVETGKPNVVPRLDEEPLFLDRTQSRVEENKEE
EEECCCCCCHHHHHHCCEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHC
LCFICVPIMVGSEVIGTLSADRRISVPTENGNKRRKSDRIDILQYYVDLLSIIAAMIAQA
EEEEEEHHHHCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRIKQLDNEEKSTDAPEHPSHPEHSGYPIAEGEFVEDVPDADRPANIIGNTKPMMSLFRM
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHCCCCHHHHHHHHH
IDKISKTNATALILGESGVGKELVANAIHYKSMRNGKPFIKFNCAALPESIVESELFGHE
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCC
KGAFTGAASLRRGRFELADTGTIFLDEVGELSLSTQAKLLRIIQEKEFERLGGAKTVKTD
CCCCCCHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
VRIIAATNRNLEEQIQQGSFREDLYYRLNIFPITVPPLRERKTDILLLADYFVEKFNTAN
EEEEEECCCCHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHCCCC
HKGIRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSGT
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCC
PYIGDLQQKLDSIEKEMIIEALKRTKGNMTKAAAQLGLSDRIMGLRMKKFNIDYKKFRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHEEHHHCCCCHHHCCC
>Mature Secondary Structure
MLIAHEQEQSSISLLAEVSRTVNNEEDISKVLRLVLFILSEHMNMLRGMVTILDKSTGEM
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VINESFGLSEEQSQLGRYKIGEGIIGQVVETGKPNVVPRLDEEPLFLDRTQSRVEENKEE
EEECCCCCCHHHHHHCCEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHC
LCFICVPIMVGSEVIGTLSADRRISVPTENGNKRRKSDRIDILQYYVDLLSIIAAMIAQA
EEEEEEHHHHCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRIKQLDNEEKSTDAPEHPSHPEHSGYPIAEGEFVEDVPDADRPANIIGNTKPMMSLFRM
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHCCCCHHHHHHHHH
IDKISKTNATALILGESGVGKELVANAIHYKSMRNGKPFIKFNCAALPESIVESELFGHE
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCC
KGAFTGAASLRRGRFELADTGTIFLDEVGELSLSTQAKLLRIIQEKEFERLGGAKTVKTD
CCCCCCHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
VRIIAATNRNLEEQIQQGSFREDLYYRLNIFPITVPPLRERKTDILLLADYFVEKFNTAN
EEEEEECCCCHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHCCCC
HKGIRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSGT
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCC
PYIGDLQQKLDSIEKEMIIEALKRTKGNMTKAAAQLGLSDRIMGLRMKKFNIDYKKFRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHEEHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]