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The map label for this gene is leuA2 [H]

Identifier: 193213259

GI number: 193213259

Start: 1763952

End: 1765112

Strand: Direct

Name: leuA2 [H]

Synonym: Cpar_1614

Alternate gene names: 193213259

Gene position: 1763952-1765112 (Clockwise)

Preceding gene: 193213258

Following gene: 193213260

Centisome position: 77.05

GC content: 62.62

Gene sequence:

>1161_bases
ATGCCAGCCCAAACCACATCCGATACCGGATCGATCCTCAAGCCGTGGATCATCGATACGACCCTCCGGGACGGAGAGCA
GGCGCCGGGCGTGGTGTTCAATCCGCATGAGAAAACACGCATCGCGGAGCTGCTGGCCGAAGCCGGGGTTGACGAAATCG
AGATCGGCTATCCGGCAATCAGCGAAGCTGAGCGCAAGGTAATCCGCGAGATCGCCGCCCTGAACCTGCCCGTGCGGCTG
ACCGCGTGGTCGCGCGCCAACATGAACGACATCGAACTGGCCGCAAAGAGTGGAACCGGTGCGGTGCACATCAGCTTCCC
GATTTCGCAGCTCTACATGGAGCTGATGAAAAAGGAGTACGACTGGGTGCAGGAGCAGCTCCACGCAATGATTTCGAAAG
CTCGCGGCCTGTTCGACTTCGTGAGCGTCGGCGGGCAGGATGCCACGCGAGCCGATCTTTCGCTGCTGCAACGATTCATG
AGCGACGCGGAAGCCGCCGGAGCTGAACGATTGCGCATCGCCGACACGGTGGGCATTTCAACGCCCGGCACGGTGATGGC
GCTTGGCGCGGCGTTGCGCGAGCACTCTGCGCTGCCGCTTGAATTCCACGCGCACAACGACCTCGGCATGGCGACCGCCA
ACGCCTTCACGGCGCTCGAAGCGGGGTTCGAAGCCGTCAGCGTGTCGGTAACCGGTCTCGGCGAAAGGGCGGGCAACGCG
GCGCTCGAAGAGCTGGCGATGGCGCTTGCGCTTTCCGGCCGATTCGAGTCTCGGCTCGACACCGCGATGCTTTCGACACT
GTGCGATACGGTCGCCCTGGCCGCGGGGCGGGCGATTCAGGATCAGAAACCGGTCGTTGGGCGCTCGGCGTTCCAGCACG
AATCGGGCATCCATTGCGCGGCCTTGCTGCAAGACCCGCTCTCCTACCAGCCATTCCTGCCGTCACAGGTCGGACGTGGC
GACTTCGAGATGGTCATCGGCAAGCATTCGGGCACGGCGGCTATCATGGAGCACTTCCGGAGAAAAGGGATCGAGATGTC
GCGTGACGAAGCGCGGGCACTGCTCGAACTGATCAGAAATCACGCCGACCGCCTCAAACGCTCGCTCAGGACGGACGAAA
TCGATGCCATCCGCGAAGGCACACTCATCAAGCAAGCTTAA

Upstream 100 bases:

>100_bases
CCCAAAACCCTTTTACACCCTAATTATTTTAGGATTTGTTTTATATTCACCTTAATCAAAAGACTCGAAAAGGTCGAAAA
GCCAGAAAAAAAAGACGTCC

Downstream 100 bases:

>100_bases
CTATCGAATAACAAGTCTTCATGCTCATAGCGCACGAACAGGAACAGAGCAGCATCAGCCTTCTTGCCGAAGTAAGCAGG
ACCGTCAACAACGAAGAGGA

Product: homocitrate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MPAQTTSDTGSILKPWIIDTTLRDGEQAPGVVFNPHEKTRIAELLAEAGVDEIEIGYPAISEAERKVIREIAALNLPVRL
TAWSRANMNDIELAAKSGTGAVHISFPISQLYMELMKKEYDWVQEQLHAMISKARGLFDFVSVGGQDATRADLSLLQRFM
SDAEAAGAERLRIADTVGISTPGTVMALGAALREHSALPLEFHAHNDLGMATANAFTALEAGFEAVSVSVTGLGERAGNA
ALEELAMALALSGRFESRLDTAMLSTLCDTVALAAGRAIQDQKPVVGRSAFQHESGIHCAALLQDPLSYQPFLPSQVGRG
DFEMVIGKHSGTAAIMEHFRRKGIEMSRDEARALLELIRNHADRLKRSLRTDEIDAIREGTLIKQA

Sequences:

>Translated_386_residues
MPAQTTSDTGSILKPWIIDTTLRDGEQAPGVVFNPHEKTRIAELLAEAGVDEIEIGYPAISEAERKVIREIAALNLPVRL
TAWSRANMNDIELAAKSGTGAVHISFPISQLYMELMKKEYDWVQEQLHAMISKARGLFDFVSVGGQDATRADLSLLQRFM
SDAEAAGAERLRIADTVGISTPGTVMALGAALREHSALPLEFHAHNDLGMATANAFTALEAGFEAVSVSVTGLGERAGNA
ALEELAMALALSGRFESRLDTAMLSTLCDTVALAAGRAIQDQKPVVGRSAFQHESGIHCAALLQDPLSYQPFLPSQVGRG
DFEMVIGKHSGTAAIMEHFRRKGIEMSRDEARALLELIRNHADRLKRSLRTDEIDAIREGTLIKQA
>Mature_385_residues
PAQTTSDTGSILKPWIIDTTLRDGEQAPGVVFNPHEKTRIAELLAEAGVDEIEIGYPAISEAERKVIREIAALNLPVRLT
AWSRANMNDIELAAKSGTGAVHISFPISQLYMELMKKEYDWVQEQLHAMISKARGLFDFVSVGGQDATRADLSLLQRFMS
DAEAAGAERLRIADTVGISTPGTVMALGAALREHSALPLEFHAHNDLGMATANAFTALEAGFEAVSVSVTGLGERAGNAA
LEELAMALALSGRFESRLDTAMLSTLCDTVALAAGRAIQDQKPVVGRSAFQHESGIHCAALLQDPLSYQPFLPSQVGRGD
FEMVIGKHSGTAAIMEHFRRKGIEMSRDEARALLELIRNHADRLKRSLRTDEIDAIREGTLIKQA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=362, Percent_Identity=30.3867403314917, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=30.6010928961749, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=30.327868852459, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324225, Length=414, Percent_Identity=23.4299516908213, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324682, Length=419, Percent_Identity=23.1503579952267, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41810; Mature: 41679

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQTTSDTGSILKPWIIDTTLRDGEQAPGVVFNPHEKTRIAELLAEAGVDEIEIGYPAI
CCCCCCCCCCCCCCCHHEECHHCCCCCCCCCEECCCHHHHHHHHHHHCCCCEEECCCCCH
SEAERKVIREIAALNLPVRLTAWSRANMNDIELAAKSGTGAVHISFPISQLYMELMKKEY
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEECCCCCEEEEECCHHHHHHHHHHHHH
DWVQEQLHAMISKARGLFDFVSVGGQDATRADLSLLQRFMSDAEAAGAERLRIADTVGIS
HHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCC
TPGTVMALGAALREHSALPLEFHAHNDLGMATANAFTALEAGFEAVSVSVTGLGERAGNA
CCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHH
ALEELAMALALSGRFESRLDTAMLSTLCDTVALAAGRAIQDQKPVVGRSAFQHESGIHCA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHH
ALLQDPLSYQPFLPSQVGRGDFEMVIGKHSGTAAIMEHFRRKGIEMSRDEARALLELIRN
HHHHCCCCCCCCCCHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HADRLKRSLRTDEIDAIREGTLIKQA
HHHHHHHHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PAQTTSDTGSILKPWIIDTTLRDGEQAPGVVFNPHEKTRIAELLAEAGVDEIEIGYPAI
CCCCCCCCCCCCCCHHEECHHCCCCCCCCCEECCCHHHHHHHHHHHCCCCEEECCCCCH
SEAERKVIREIAALNLPVRLTAWSRANMNDIELAAKSGTGAVHISFPISQLYMELMKKEY
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEECCCCCEEEEECCHHHHHHHHHHHHH
DWVQEQLHAMISKARGLFDFVSVGGQDATRADLSLLQRFMSDAEAAGAERLRIADTVGIS
HHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCC
TPGTVMALGAALREHSALPLEFHAHNDLGMATANAFTALEAGFEAVSVSVTGLGERAGNA
CCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHH
ALEELAMALALSGRFESRLDTAMLSTLCDTVALAAGRAIQDQKPVVGRSAFQHESGIHCA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHH
ALLQDPLSYQPFLPSQVGRGDFEMVIGKHSGTAAIMEHFRRKGIEMSRDEARALLELIRN
HHHHCCCCCCCCCCHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HADRLKRSLRTDEIDAIREGTLIKQA
HHHHHHHHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]