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The map label for this gene is tilS/hprT [H]

Identifier: 193213258

GI number: 193213258

Start: 1763101

End: 1763628

Strand: Direct

Name: tilS/hprT [H]

Synonym: Cpar_1613

Alternate gene names: 193213258

Gene position: 1763101-1763628 (Clockwise)

Preceding gene: 193213255

Following gene: 193213259

Centisome position: 77.02

GC content: 60.23

Gene sequence:

>528_bases
ATGATGACGTTGCCCCAAAAAGAGCTCATCTCCGCCGAACGGCTTCAAGAGCGCGTCGCTGAGCTGGCTCGGGAGATTTC
CGACGATCTGGAAGGGATTGATCTGCTGACGGTGGTTTGCGTGCTGAAGGGGGCGTTCATCTTCACAGCCGATCTGGTGC
GTCACCTGACCCTGCCCTGCCGCATCGAGTTCATCCGGGCTTCGAGTTATGGCGAAGGGCGCACCTCATCCGGCAAGGTC
AAGCTCGAACATCATGAAACCATCGAGCTTGAAGGCAAGCACGTGCTGCTGGTGGAGGATATTCTCGACACGGGGCGCAC
CCTCTCGCGCATCGTAGCCGAACTGGAACAGCAGCGACCGGCCTCCATGCGCGTCTGCGCCCTGCTCGACAAGCCCTCGC
GCCGGAGCGTCCTATTCGAAGCGAACTATACGGGCTTCACGATTCCTGACCACTTCGTCGTCGGCTACGGCCTCGACGCT
GACGAGCGGTATCGCGAACTGCCGTTCATCGCCATCGAAAACCGCTGA

Upstream 100 bases:

>100_bases
GGGCGTGAGCATTGTCGGCACTGCAAGCAACATTAACGAAAAACAATGGTAAACGAGCTATATTCTGTGAAGCCCCATCG
AACCACTACCATCCGGAATC

Downstream 100 bases:

>100_bases
AGGGTGGGGCCGAGAAGCAGTCCTTATTTCGAGCCACTCGTATCCAGCCCGCTACCGGAAATGAAGCCTTGAACAACGTC
ACTACCCTATCCGTCATTCC

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MMTLPQKELISAERLQERVAELAREISDDLEGIDLLTVVCVLKGAFIFTADLVRHLTLPCRIEFIRASSYGEGRTSSGKV
KLEHHETIELEGKHVLLVEDILDTGRTLSRIVAELEQQRPASMRVCALLDKPSRRSVLFEANYTGFTIPDHFVVGYGLDA
DERYRELPFIAIENR

Sequences:

>Translated_175_residues
MMTLPQKELISAERLQERVAELAREISDDLEGIDLLTVVCVLKGAFIFTADLVRHLTLPCRIEFIRASSYGEGRTSSGKV
KLEHHETIELEGKHVLLVEDILDTGRTLSRIVAELEQQRPASMRVCALLDKPSRRSVLFEANYTGFTIPDHFVVGYGLDA
DERYRELPFIAIENR
>Mature_175_residues
MMTLPQKELISAERLQERVAELAREISDDLEGIDLLTVVCVLKGAFIFTADLVRHLTLPCRIEFIRASSYGEGRTSSGKV
KLEHHETIELEGKHVLLVEDILDTGRTLSRIVAELEQQRPASMRVCALLDKPSRRSVLFEANYTGFTIPDHFVVGYGLDA
DERYRELPFIAIENR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI9910262, Length=191, Percent_Identity=36.1256544502618, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI4504483, Length=168, Percent_Identity=35.7142857142857, Blast_Score=111, Evalue=4e-25,
Organism=Escherichia coli, GI87081689, Length=165, Percent_Identity=43.030303030303, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17510817, Length=166, Percent_Identity=36.144578313253, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 19840; Mature: 19840

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLPQKELISAERLQERVAELAREISDDLEGIDLLTVVCVLKGAFIFTADLVRHLTLPC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCE
RIEFIRASSYGEGRTSSGKVKLEHHETIELEGKHVLLVEDILDTGRTLSRIVAELEQQRP
EEEEEEECCCCCCCCCCCEEEEECCCEEEECCCEEEEEHHHHHCCHHHHHHHHHHHHCCC
ASMRVCALLDKPSRRSVLFEANYTGFTIPDHFVVGYGLDADERYRELPFIAIENR
CHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHCCEEEECCC
>Mature Secondary Structure
MMTLPQKELISAERLQERVAELAREISDDLEGIDLLTVVCVLKGAFIFTADLVRHLTLPC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCE
RIEFIRASSYGEGRTSSGKVKLEHHETIELEGKHVLLVEDILDTGRTLSRIVAELEQQRP
EEEEEEECCCCCCCCCCCEEEEECCCEEEECCCEEEEEHHHHHCCHHHHHHHHHHHHCCC
ASMRVCALLDKPSRRSVLFEANYTGFTIPDHFVVGYGLDADERYRELPFIAIENR
CHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]