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The map label for this gene is raiI

Identifier: 190894997

GI number: 190894997

Start: 716159

End: 716797

Strand: Reverse

Name: raiI

Synonym: RHECIAT_PC0000663

Alternate gene names: 190894997

Gene position: 716797-716159 (Counterclockwise)

Preceding gene: 190895007

Following gene: 190894996

Centisome position: 65.67

GC content: 59.94

Gene sequence:

>639_bases
ATGTTGCGGATACTCACCAAAGACATGCTCGAGACCGATCGACGGGCTTTCGATGAAATGTTTCGGGCTCGCGCCGCCGT
TTTTCGAGATCGTCTGGGATGGCAGGTCGATGTCCGGGATCAATGGGAGAGGGACCGATACGACGAGGCTGAGGATCCCG
TCTATCTCGTCACGCAACAACCTTCCGGCACGCTGACGGGTTCGCTGCGCCTGCTGCCGACCACCGGCGCGACGATGCTC
AAAAGCGAGTTCCGGCATTTCTTCGATCAGCCTATCGACGTTGATAGCCCGACGACCTGGGAATGTACCCGCTTTTGCCT
TCATCCGCATGCCGGCGATATGAAGCAATCGCGCGCAGTCGCCACGGAGCTGCTCTCAGGGCTTTGCGATCTTGCGCTCG
ACACCGGCATCGAAAACATTGTCGGCGTCTATGACGTCGCGATGGTCGCGGTGTACCGGAGGATCGGCTGGAGGCCGACG
CCGCTTGCCCGATCCCGGCCCGAGATCGGCAAGCTGTATGTTGGTCTATGGGATGTGACGGCGGACAATTGTCGGACGCT
TAGGGCCAACCTGTCCCGACTTCTGGAGCAAGCCTCTCCCTATCCTGCCAGAGTCCTTGTCGATGGCGGCATGCGATAG

Upstream 100 bases:

>100_bases
AAGGGGACGGCAAAGCCAAGCACCGGTCAACCTGGTGAAATATCCAGATTGCGCCCGGTAGGCCTCCTGTTGAACCGTCA
GCACATCAGGAGGGACGGAT

Downstream 100 bases:

>100_bases
GCGCCGTGTCCCAACGCCCAGCAGTGGGATAGGTCTCACCGGTCTTTAACTTGTTCTTCATATCGCGCCATGGTTGCCAA
AAATATACTGTTCTCGGTAA

Product: autoinducer synthesis protein

Products: NA

Alternate protein names: Autoinducer synthesis protein raiI

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQQPSGTLTGSLRLLPTTGATML
KSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGDMKQSRAVATELLSGLCDLALDTGIENIVGVYDVAMVAVYRRIGWRPT
PLARSRPEIGKLYVGLWDVTADNCRTLRANLSRLLEQASPYPARVLVDGGMR

Sequences:

>Translated_212_residues
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQQPSGTLTGSLRLLPTTGATML
KSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGDMKQSRAVATELLSGLCDLALDTGIENIVGVYDVAMVAVYRRIGWRPT
PLARSRPEIGKLYVGLWDVTADNCRTLRANLSRLLEQASPYPARVLVDGGMR
>Mature_212_residues
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQQPSGTLTGSLRLLPTTGATML
KSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGDMKQSRAVATELLSGLCDLALDTGIENIVGVYDVAMVAVYRRIGWRPT
PLARSRPEIGKLYVGLWDVTADNCRTLRANLSRLLEQASPYPARVLVDGGMR

Specific function: Required for the synthesis of autoinducer molecules which bind to raiR and that are involved in the restriction of nodule number

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RAII_RHIET (O54451)

Other databases:

- EMBL:   AY708656
- ProteinModelPortal:   O54451
- BRENDA:   2.3.1.184
- GO:   GO:0009877
- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311
- Gene3D:   G3DSA:3.40.630.30
- PRINTS:   PR01549

Pfam domain/function: PF00765 Autoind_synth; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.1.184

Molecular weight: Translated: 24209; Mature: 24209

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00949 AUTOINDUCER_SYNTH_1; PS51187 AUTOINDUCER_SYNTH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEEEEEC
PSGTLTGSLRLLPTTGATMLKSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGDMKQSRAV
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
ATELLSGLCDLALDTGIENIVGVYDVAMVAVYRRIGWRPTPLARSRPEIGKLYVGLWDVT
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECC
ADNCRTLRANLSRLLEQASPYPARVLVDGGMR
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEEEEEC
PSGTLTGSLRLLPTTGATMLKSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGDMKQSRAV
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
ATELLSGLCDLALDTGIENIVGVYDVAMVAVYRRIGWRPTPLARSRPEIGKLYVGLWDVT
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECC
ADNCRTLRANLSRLLEQASPYPARVLVDGGMR
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473034