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The map label for this gene is thcR [H]

Identifier: 190894992

GI number: 190894992

Start: 711135

End: 712034

Strand: Reverse

Name: thcR [H]

Synonym: RHECIAT_PC0000658

Alternate gene names: 190894992

Gene position: 712034-711135 (Counterclockwise)

Preceding gene: 190894993

Following gene: 190894990

Centisome position: 65.23

GC content: 60.11

Gene sequence:

>900_bases
TTGTCGACGCCGAAAGCTTCGATCAAGATTACTGCCGATGGCAATATGCCGATCGCATACAGCTGCAATTTCATCTCGCT
GGGAGATGTGAGCGTTGGCGATTGCGCTTATGAGGGCACGATCTTCTCCAAGCGGGAAGGGATCAGCGAAAAGATGGCTA
TATTTCTGCCGACGGAAGGGAATATGTCTTTCGGGGGAAGGCAGGGGCCGATCTATTCGGTCCCCGGCCGCGGTACAATC
CTGGAGCCCGGCCGTGCCAGGGGCACCGCCATTTTCGGCACACGCCGTCATCTCTGCCTGTTCATCGATCAGGCGAGCAT
CTCCAGGCGCCTCACGCATATGCTGGAGCGGACGATCAGCGCCGATCTCGATATTCATCCCGATATCGATCTGACGGCCG
GTGCGGGACTCGCCTTGCAGAAAATCGTCGCCAACCTTCACGGCGGTCTTACCGGCAACGGGCCGTTGCGCCGCTCGCCG
CTCGCCGTCAGTTCGCTTAGCGACGCGGCGATCTATCTGCTTCTGGAGAGTTGTCCCAATCGCTATTCGGATGAGCTCGG
GGCGAGCGCGCCGATGCCGGCTCCTCGGCACGTGAAATGGGCCATCGATTTCATGCACGAACATATCGCCGAACCGATCT
CGCTCAGCGAGATTGCGGCGGCGGCCAAGGTGAGCACCCGGACTTTACAGCAAGGCTTCCGGCAGTTCAGGAACACCACC
CCGATCGCCTACCTGCACGAAATCCGGATGGTCGCCGCTCATCGCGATCTGCTCGAAGCCGGCTCGAAGCAGGCCGTCGC
CGATATCGCGCTCAGATGGGGCTTTACGCATCTCGGACGTTTTGCCGCCGAACATAAGAAGCGGTTCGGCCAGCTGCCGT
CGCAGACCTTGAAGGTCTGA

Upstream 100 bases:

>100_bases
GCCTGCCGATACCAGTTCCGCCCGCCACTCTGCAACGCAGAGCTTCCCGTTTCGGGAAACGGCCGAAAGCGGGACGGATC
CGGACGAACTTTCGGAAGCA

Downstream 100 bases:

>100_bases
TCTAGGCAAGCCTGCCCATGGCCGGCCGCATATTCTGCGGTTGGTCATGGGCGTGATCCGGGCTTCGCGTCAGCCGGCTG
CGATGATCGTCAACGTGCGG

Product: putative AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSTPKASIKITADGNMPIAYSCNFISLGDVSVGDCAYEGTIFSKREGISEKMAIFLPTEGNMSFGGRQGPIYSVPGRGTI
LEPGRARGTAIFGTRRHLCLFIDQASISRRLTHMLERTISADLDIHPDIDLTAGAGLALQKIVANLHGGLTGNGPLRRSP
LAVSSLSDAAIYLLLESCPNRYSDELGASAPMPAPRHVKWAIDFMHEHIAEPISLSEIAAAAKVSTRTLQQGFRQFRNTT
PIAYLHEIRMVAAHRDLLEAGSKQAVADIALRWGFTHLGRFAAEHKKRFGQLPSQTLKV

Sequences:

>Translated_299_residues
MSTPKASIKITADGNMPIAYSCNFISLGDVSVGDCAYEGTIFSKREGISEKMAIFLPTEGNMSFGGRQGPIYSVPGRGTI
LEPGRARGTAIFGTRRHLCLFIDQASISRRLTHMLERTISADLDIHPDIDLTAGAGLALQKIVANLHGGLTGNGPLRRSP
LAVSSLSDAAIYLLLESCPNRYSDELGASAPMPAPRHVKWAIDFMHEHIAEPISLSEIAAAAKVSTRTLQQGFRQFRNTT
PIAYLHEIRMVAAHRDLLEAGSKQAVADIALRWGFTHLGRFAAEHKKRFGQLPSQTLKV
>Mature_298_residues
STPKASIKITADGNMPIAYSCNFISLGDVSVGDCAYEGTIFSKREGISEKMAIFLPTEGNMSFGGRQGPIYSVPGRGTIL
EPGRARGTAIFGTRRHLCLFIDQASISRRLTHMLERTISADLDIHPDIDLTAGAGLALQKIVANLHGGLTGNGPLRRSPL
AVSSLSDAAIYLLLESCPNRYSDELGASAPMPAPRHVKWAIDFMHEHIAEPISLSEIAAAAKVSTRTLQQGFRQFRNTTP
IAYLHEIRMVAAHRDLLEAGSKQAVADIALRWGFTHLGRFAAEHKKRFGQLPSQTLKV

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=98, Percent_Identity=36.734693877551, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32460; Mature: 32329

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPKASIKITADGNMPIAYSCNFISLGDVSVGDCAYEGTIFSKREGISEKMAIFLPTEG
CCCCCCEEEEEECCCCCEEEECCEEEECCCCCCCEEECCCEEHHHCCCCCCEEEEEECCC
NMSFGGRQGPIYSVPGRGTILEPGRARGTAIFGTRRHLCLFIDQASISRRLTHMLERTIS
CCCCCCCCCCEEECCCCCCEECCCCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHH
ADLDIHPDIDLTAGAGLALQKIVANLHGGLTGNGPLRRSPLAVSSLSDAAIYLLLESCPN
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
RYSDELGASAPMPAPRHVKWAIDFMHEHIAEPISLSEIAAAAKVSTRTLQQGFRQFRNTT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PIAYLHEIRMVAAHRDLLEAGSKQAVADIALRWGFTHLGRFAAEHKKRFGQLPSQTLKV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
STPKASIKITADGNMPIAYSCNFISLGDVSVGDCAYEGTIFSKREGISEKMAIFLPTEG
CCCCCEEEEEECCCCCEEEECCEEEECCCCCCCEEECCCEEHHHCCCCCCEEEEEECCC
NMSFGGRQGPIYSVPGRGTILEPGRARGTAIFGTRRHLCLFIDQASISRRLTHMLERTIS
CCCCCCCCCCEEECCCCCCEECCCCCCCEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHH
ADLDIHPDIDLTAGAGLALQKIVANLHGGLTGNGPLRRSPLAVSSLSDAAIYLLLESCPN
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
RYSDELGASAPMPAPRHVKWAIDFMHEHIAEPISLSEIAAAAKVSTRTLQQGFRQFRNTT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PIAYLHEIRMVAAHRDLLEAGSKQAVADIALRWGFTHLGRFAAEHKKRFGQLPSQTLKV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]