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The map label for this gene is carA [H]

Identifier: 190571543

GI number: 190571543

Start: 1250682

End: 1251770

Strand: Direct

Name: carA [H]

Synonym: WPa_1163

Alternate gene names: 190571543

Gene position: 1250682-1251770 (Clockwise)

Preceding gene: 190571540

Following gene: 190571544

Centisome position: 84.37

GC content: 34.44

Gene sequence:

>1089_bases
ATGCAGGATGCAGCTTTAGTTTTACAAGATGGCAAGTACTTTTTGGGAAAATCGGTAGGTAAAAGAGGCAAATGTATAGG
TGAAGTTTGTTTTACCACCGGTATGACAGGTTATCAGCATACTATAACAGATCCTTCTTTTGCTGATCAAATAATAGCGT
TCACTTTTCCTCATATTGGTAATGTTGGAATAAATCACAAAGATAATGAAGGAGAAAAAGTTTTTGCAAGTGGTGTGGTT
ATGCGTGAACTTTCTCCTATGTCTCACTCTTCTTCATATATTAGTTTAAATGATTGGTTGGAGAAAAATAATGTAGTTGG
GATATCGGGAATTGATACTAGAGCTTTGACGAGATATTTGAGAAAACATGGATCTCAAAATGGAATGATATGTCCGTCAA
GTGAGGTACATATATTAGATGAATTGAAGAAATACAAATCTGTAAATGGAATAGAAATAACTAAGAAAGTCAGTTTAAGT
AATAACTTTCAAAGCGTTCTTAATGCAAAATATAGGGTTGTAATTGTTGATTTCGGTGTAAAAAATAGTATAATCTCACG
CTTAGTAGAACTTGATTGTGCGGTTGAATTAGTCAAACCAGATACAGGTTTTGCTCACAAAATATTAAGCATGAATCCAG
ATGGTATAGTGCTTTCAAATGGCCCTGGTGATCCACAAGAGATAGGAGAGAGTGTAACTTCAGAAATAGAAATTATTGTG
AAATCTAAAATACCAGTTTTTGGCATATGCCTGGGCCATCAATTACTTGCAGTTACTTTGGGAGCAAAGACTGTCAAGAT
GGATATTGGTCATCGAGGGAGTAATCATCCAGTTTATGATCTGGAGAGTAAAAAAGTTGAGATAACTAGCCAAAATCATG
GTTTTGTTGTTGATTCAGCTTCTCTTCCAAGTAACGTTGAAGTTACTCATATTTCTCTATTTGATAATAGTGTAGAGGGG
ATAATGATGAAGGATTACCCAGTCTTTTCTACTCAATACCATCCAGAAGAAGCGCCAGGTACACATGATTCACATTACTT
ATTTGGGCGTTTTATTGACAATATTTTGTTATATAAAAGTAAAATCTAA

Upstream 100 bases:

>100_bases
ATAATTTTGAATTTTATATAAACTTAAAGGGAAATTTAGTATTTGCTTACTTGTAATTTAAAATTCTTATATAATATTTT
AAATGAGTTGAGAGAATTAA

Downstream 100 bases:

>100_bases
CTGAAATCAGTTAGATTAGCTTTTATTACTTGATCTTTTGGATTTCGATGTTTAATATTAGGAATAATAAAACTTAAGTT
AAGGTAGTGTTCTCTTAACA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQDAALVLQDGKYFLGKSVGKRGKCIGEVCFTTGMTGYQHTITDPSFADQIIAFTFPHIGNVGINHKDNEGEKVFASGVV
MRELSPMSHSSSYISLNDWLEKNNVVGISGIDTRALTRYLRKHGSQNGMICPSSEVHILDELKKYKSVNGIEITKKVSLS
NNFQSVLNAKYRVVIVDFGVKNSIISRLVELDCAVELVKPDTGFAHKILSMNPDGIVLSNGPGDPQEIGESVTSEIEIIV
KSKIPVFGICLGHQLLAVTLGAKTVKMDIGHRGSNHPVYDLESKKVEITSQNHGFVVDSASLPSNVEVTHISLFDNSVEG
IMMKDYPVFSTQYHPEEAPGTHDSHYLFGRFIDNILLYKSKI

Sequences:

>Translated_362_residues
MQDAALVLQDGKYFLGKSVGKRGKCIGEVCFTTGMTGYQHTITDPSFADQIIAFTFPHIGNVGINHKDNEGEKVFASGVV
MRELSPMSHSSSYISLNDWLEKNNVVGISGIDTRALTRYLRKHGSQNGMICPSSEVHILDELKKYKSVNGIEITKKVSLS
NNFQSVLNAKYRVVIVDFGVKNSIISRLVELDCAVELVKPDTGFAHKILSMNPDGIVLSNGPGDPQEIGESVTSEIEIIV
KSKIPVFGICLGHQLLAVTLGAKTVKMDIGHRGSNHPVYDLESKKVEITSQNHGFVVDSASLPSNVEVTHISLFDNSVEG
IMMKDYPVFSTQYHPEEAPGTHDSHYLFGRFIDNILLYKSKI
>Mature_362_residues
MQDAALVLQDGKYFLGKSVGKRGKCIGEVCFTTGMTGYQHTITDPSFADQIIAFTFPHIGNVGINHKDNEGEKVFASGVV
MRELSPMSHSSSYISLNDWLEKNNVVGISGIDTRALTRYLRKHGSQNGMICPSSEVHILDELKKYKSVNGIEITKKVSLS
NNFQSVLNAKYRVVIVDFGVKNSIISRLVELDCAVELVKPDTGFAHKILSMNPDGIVLSNGPGDPQEIGESVTSEIEIIV
KSKIPVFGICLGHQLLAVTLGAKTVKMDIGHRGSNHPVYDLESKKVEITSQNHGFVVDSASLPSNVEVTHISLFDNSVEG
IMMKDYPVFSTQYHPEEAPGTHDSHYLFGRFIDNILLYKSKI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=369, Percent_Identity=37.3983739837398, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI21361331, Length=370, Percent_Identity=34.8648648648649, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI169790915, Length=370, Percent_Identity=34.8648648648649, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=45.4068241469816, Blast_Score=328, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI193204318, Length=374, Percent_Identity=33.9572192513369, Blast_Score=210, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6324878, Length=376, Percent_Identity=37.5, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=32.3979591836735, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI45555749, Length=376, Percent_Identity=34.0425531914894, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24642586, Length=376, Percent_Identity=34.0425531914894, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39785; Mature: 39785

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDAALVLQDGKYFLGKSVGKRGKCIGEVCFTTGMTGYQHTITDPSFADQIIAFTFPHIG
CCCCEEEEECCCEEEHHCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHEEEEECCCCC
NVGINHKDNEGEKVFASGVVMRELSPMSHSSSYISLNDWLEKNNVVGISGIDTRALTRYL
CCCCCCCCCCCCEEEECCCCEECCCCCCCCCCEEEHHHHHCCCCEEEECCCCHHHHHHHH
RKHGSQNGMICPSSEVHILDELKKYKSVNGIEITKKVSLSNNFQSVLNAKYRVVIVDFGV
HHHCCCCCEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCEEEEEEECCC
KNSIISRLVELDCAVELVKPDTGFAHKILSMNPDGIVLSNGPGDPQEIGESVTSEIEIIV
HHHHHHHHHHHHHEEEEECCCCCCHHHHEEECCCCEEEECCCCCHHHHHHHHHHHEEEEE
KSKIPVFGICLGHQLLAVTLGAKTVKMDIGHRGSNHPVYDLESKKVEITSQNHGFVVDSA
ECCCCEEEEEHHHHEEEEEECCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCEEEECC
SLPSNVEVTHISLFDNSVEGIMMKDYPVFSTQYHPEEAPGTHDSHYLFGRFIDNILLYKS
CCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
KI
CC
>Mature Secondary Structure
MQDAALVLQDGKYFLGKSVGKRGKCIGEVCFTTGMTGYQHTITDPSFADQIIAFTFPHIG
CCCCEEEEECCCEEEHHCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHEEEEECCCCC
NVGINHKDNEGEKVFASGVVMRELSPMSHSSSYISLNDWLEKNNVVGISGIDTRALTRYL
CCCCCCCCCCCCEEEECCCCEECCCCCCCCCCEEEHHHHHCCCCEEEECCCCHHHHHHHH
RKHGSQNGMICPSSEVHILDELKKYKSVNGIEITKKVSLSNNFQSVLNAKYRVVIVDFGV
HHHCCCCCEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCEEEEEEECCC
KNSIISRLVELDCAVELVKPDTGFAHKILSMNPDGIVLSNGPGDPQEIGESVTSEIEIIV
HHHHHHHHHHHHHEEEEECCCCCCHHHHEEECCCCEEEECCCCCHHHHHHHHHHHEEEEE
KSKIPVFGICLGHQLLAVTLGAKTVKMDIGHRGSNHPVYDLESKKVEITSQNHGFVVDSA
ECCCCEEEEEHHHHEEEEEECCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCEEEECC
SLPSNVEVTHISLFDNSVEGIMMKDYPVFSTQYHPEEAPGTHDSHYLFGRFIDNILLYKS
CCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
KI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]