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The map label for this gene is tuf [H]

Identifier: 190571544

GI number: 190571544

Start: 1252115

End: 1253287

Strand: Direct

Name: tuf [H]

Synonym: WPa_1164

Alternate gene names: 190571544

Gene position: 1252115-1253287 (Clockwise)

Preceding gene: 190571543

Following gene: 190571545

Centisome position: 84.46

GC content: 40.75

Gene sequence:

>1173_bases
ATGACAGCGATAGTAGAAGCATTTGGAAAGCCGCATGTAAATGTGGGAACGATAGGACATGTGGATCATGGGAAGACAAC
GTTAACAGCGGCGATAACAAAGCATTATGGTAATTTTGTAGCATATGATCAAATAGATAAAGCGCCAGAAGAAAGAAAGA
GAGGGATAACAATAGCAACAGCGCATGTTGAATATCAAACAGAAAAGAGACACTATGCACACGTTGATTGCCCTGGACAT
GCTGATTATGTAAAGAATATGATAGTAGGTGCAGCACAGATGGATGCAGCGATATTGGTAGTGTCAGGGGTTGATGGGCC
GATGCCACAAACGAGAGAGCATATATTGCTGGCAAAACAGGTGGGTGTTGGATATATCGTGGTATATATAAATAAAGCTG
ATGTTGCTGATGCTGATATGATAGATTTGGTAGAGATGGAAGTGAGAGAGTTGCTGAGTAAGTATGGATTTCCAGGTGAT
GAAGTGCCTATGATAGTTGGGTCTGCGTTAAAAGCATTAGAGGATGATAGTAGTGAGTATGGAAAGAAATCAATAGATAA
ATTGATGGAGAAGTTAGATGAGTATGTGGCAGTACCTCCAAGGCCTATAGATTTGCCGTTTTTGTTGCCAATAGAAGATG
TATTTTCAATATCGGGCCGAGGGACGGTAGTAACAGGAAGAATAGAGAAGGGGGAGATAAAGACAGGGGATGAGATAGAG
ATAATAGGTCTGAAAGCGACGCAAAAGACGATATGTACTGGTGTTGAGATGTTTAAGAAGTTGCTAGATAAGGGAAGTGC
AGGACTCAATGTAGGAATACTACTAAGAGGAACAAAGAGAGAAGAAGTGGAGAGAGGGCAAGTATTGGCAAAACCAGGGA
CAATAACCCCGCATAAGAAATTTAATGCGGAGGTGTATATATTGAAGAAAGAAGAAGGAGGAAGGCATACACCATTTTTT
GGAAATTATCAGCCACAGTTTTATTTAAGGACAACGGATGTAACTGGGAGCATAAAATTGCTAGATGGAAAGGAGATGGT
AATGCCAGGGGACAATGTAAGTATAGAAGTGGAGTTGCAAGTACCAATAGCAATGGATAAAGGATTACGTTTTGCGATAA
GAGAAGGTGGTAGAACTGTTGGTTCTGGCGTTGTTTCGGAAATTTTAGAATGA

Upstream 100 bases:

>100_bases
GGTTCGATTCCCACTATCCGCTCTTTTTTTTGTTGTATTTATATAAAATCAACATATTATTTGTTGATGTATTTATATTT
TAGGTAGAAGAGTTAAAATT

Downstream 100 bases:

>100_bases
GTATAATAACTAAGATGAAGCAAGATATATATATTAGAATCAAAGCTTTCGATTGTTCTTTGTTGGAAAAGTGTATTCGC
GAGTTTATTGATCAATTAAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTAIVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGH
ADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGD
EVPMIVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPIDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGDEIE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHKKFNAEVYILKKEEGGRHTPFF
GNYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSIEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE

Sequences:

>Translated_390_residues
MTAIVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGH
ADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGD
EVPMIVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPIDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGDEIE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHKKFNAEVYILKKEEGGRHTPFF
GNYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSIEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE
>Mature_389_residues
TAIVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGHA
DYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGDE
VPMIVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPIDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGDEIEI
IGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHKKFNAEVYILKKEEGGRHTPFFG
NYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSIEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=386, Percent_Identity=56.4766839378238, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=31.4087759815243, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=31.4087759815243, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4503471, Length=440, Percent_Identity=29.0909090909091, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI4503475, Length=441, Percent_Identity=28.5714285714286, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=30.1775147928994, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=30.1775147928994, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=30.1775147928994, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=29.585798816568, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=29.1139240506329, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.0898876404494, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI157426893, Length=160, Percent_Identity=32.5, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1790412, Length=386, Percent_Identity=69.9481865284974, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1789737, Length=386, Percent_Identity=69.9481865284974, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI2367247, Length=366, Percent_Identity=29.7814207650273, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI48994988, Length=236, Percent_Identity=31.3559322033898, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789108, Length=246, Percent_Identity=26.8292682926829, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=394, Percent_Identity=48.9847715736041, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=43.2642487046632, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=440, Percent_Identity=29.0909090909091, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=440, Percent_Identity=29.0909090909091, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.0459770114943, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.2758620689655, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=427, Percent_Identity=27.8688524590164, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=435, Percent_Identity=25.7471264367816, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=267, Percent_Identity=29.5880149812734, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=37.2413793103448, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=229, Percent_Identity=30.1310043668122, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=28.673835125448, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=402, Percent_Identity=59.7014925373134, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=29.9086757990868, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=29.9086757990868, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=32.4483775811209, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=466, Percent_Identity=23.8197424892704, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=28.5198555956679, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322359, Length=175, Percent_Identity=29.1428571428571, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363316, Length=385, Percent_Identity=55.3246753246753, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=385, Percent_Identity=55.3246753246753, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=27.5784753363229, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=27.5784753363229, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=27.5784753363229, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=27.5784753363229, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=27.5784753363229, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=27.5784753363229, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=30.6666666666667, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=31.5942028985507, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=26.3888888888889, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24647046, Length=277, Percent_Identity=28.158844765343, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=277, Percent_Identity=28.158844765343, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706572, Length=243, Percent_Identity=27.5720164609054, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42659; Mature: 42528

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIAT
CCCCHHHCCCCCCCCCEEECCCCCCEEEHHHHHHHCCCEEEEHHHHCCHHHHHCCEEEEE
AHVEYQTEKRHYAHVDCPGHADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEE
VGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGDEVPMIVGSALKALEDDSSEY
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHH
GKKSIDKLMEKLDEYVAVPPRPIDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGDEIE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEEEECCCCCCCCEEE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHKK
EEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCEEECCCCCCCCCC
FNAEVYILKKEEGGRHTPFFGNYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSIEVELQ
CCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCEEEECCCCEEEEEEEE
VPIAMDKGLRFAIREGGRTVGSGVVSEILE
EEEEECCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TAIVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIAT
CCCHHHCCCCCCCCCEEECCCCCCEEEHHHHHHHCCCEEEEHHHHCCHHHHHCCEEEEE
AHVEYQTEKRHYAHVDCPGHADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEE
VGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGDEVPMIVGSALKALEDDSSEY
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHH
GKKSIDKLMEKLDEYVAVPPRPIDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGDEIE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEEEECCCCCCCCEEE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHKK
EEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCEEECCCCCCCCCC
FNAEVYILKKEEGGRHTPFFGNYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSIEVELQ
CCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCEEEECCCCEEEEEEEE
VPIAMDKGLRFAIREGGRTVGSGVVSEILE
EEEEECCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA