| Definition | Wolbachia endosymbiont of Culex quinquefasciatus Pel, complete genome. |
|---|---|
| Accession | NC_010981 |
| Length | 1,482,455 |
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The map label for this gene is gyrB [H]
Identifier: 190571487
GI number: 190571487
Start: 1179809
End: 1182181
Strand: Reverse
Name: gyrB [H]
Synonym: WPa_1103
Alternate gene names: 190571487
Gene position: 1182181-1179809 (Counterclockwise)
Preceding gene: 190571488
Following gene: 190571486
Centisome position: 79.74
GC content: 35.78
Gene sequence:
>2373_bases ATGGAAAGTAACTACAACGCCGACGCAATAAAAATCCTAAGAGGTCTTGATGCTGTAAGAAAGCGTCCAGGTATGTATAT TGGTGACACTGATGATGGATCAGGCTTGCACCATATGGTGTATGAGGTTGTTGATAATGCGATAGATGAGTCTTTAGCTG GTTATTGTAGTAAGATTGAAGTTAGCATAAATGAAGATGGTTCAGTTTCTGTAACCGACAACGGTCGTGGCATTCCAACA GACATTCATCCAGAGGAAGGAATATCGGCAGCAGAAGTAATAATGACCCAATTGCATGCAGGAGGTAAATTTGACAGCAA TACCTATAAGGTTTCCGGAGGATTACATGGTGTTGGAATTTCAGTTGTGAATGCATTATCAAGTTGGCTGGAATTAACCA TCTGGCGCAATAAAAAAGAACATTTTATGCGCTTTGAAGATGGTGAGTCTATTGAATCTTTAAAGATAATTAATGAAAAC ACAAATAAAAGAGGAACTAGAGTAACGTTTATGCCATCAACGGGGACTTTTAGTAGTATTGATTTTAGTTACTCCACTCT TGAGAGTCGCATCAGAGAATTGGCATTTTTAAACTCAAATATTGAGATAATTTTGCGTGATCTCCGTAATAAACCACATG TAGAGTCTCATTTTAACGATAGTAAAGAGTCTGAAGATAATTTTGGTACAGCTAATTTTGTACGGTACTTAGACAAGAAT AAGACACATGTTACTAAAATTGCCAGCATTAGAGATAATGTAAAAGATCTAGGTACCAGTGTAGAAATATCAATGGAGTG GAATGATTCCTACTATGAGAATATGCTATGCTTCACAAATAATATAAGACAACGGGATGGTGGTACGCATTTGGCAGGGT TTAGATCTGCACTAACCAGATGTATCAATAACTATGCAAACAATGAAGGTTTTTTGAAGAAGGCAAAAGTGAATTTGACT GGAGAAGATGTTAGAGAAGGCTTAACTTGTGTTTTATCTCTCAAGATGCCTGATCCTAAATTTTCTTCACAGACAAAAGA TAAATTGGTCAGTTCTGAAGCGCGGGCAGTTGTAGAGAGCATAGTTTTTGACAAGTTGAGTACAATACTTGAAACTGACC CCAAGTTAGCAGCAAGTATAGTAGAAAGAGCGATTAGGTCAGCAAAAGGAAGAGAAGCCGCAAGAAAAGCACGAGAGTTA GTTAAAAATAAAAACAATATTAATATTGCAACTCTACCGGGAAAGCTTGCCGATTGTCAGGAAAAAGTTCCTGAGTTATC GGAATTGTTTATAGTAGAGGGTAATTCCGCAGGTGGTACTGCAAAGCAGGGACGTAATCGTAAAACACAGGCGGTACTTG CTTTAAGAGGGAAAATTCTAAACGTAGAACGTGCAGGGCTGGATCGTATTTTTTCATCTGCAGAAATTGGCTCTCTGATC ACGGCAATTGGGGCTGGAATAGGGAGTGAGCACTTCGATATTGAAAAAACTAGGTATCATAAAATTATCATTATGACAGA CGCAGACGTTGATGGCTCGCATATAAGAACTTTGATTCTAACTTTCTTTTTTAGACATATGCGTGAAGTAATTGAGAAAG GATATTTATACATAGCGCAGCCACCGCTCTATAAAGTTACAAAAAATGCTAAAGATACTTATATCAAAGATGATGAAACT TTTGAAGAGTATATAGTAAATTCAGCGATTAAAAGATTAACATTAAATGGTGTAGGCAAAGATTTGCGTTTCGTTTTGAA TAAGTGCCTCAGTATTTCAAACATTAGCAAAAATTATGATAGGGAAATACCAAAAGATCTCTTAGAATCATTGCTAATTT TAAGCAAAAAGAATGCTCTATTGTCTGCTGATGAGATATTAAAATATTTAAAATTGATGTATAGTGAATATAATTGGGAA GTAGAAGTAAAAGATGAGGAAATTCACATTGCTAAATTGTTTCAAGGGTTAGCAGACAAATATGTATTCTCGCTTAGTAT GCTTGAAAGCAAAGAAATACGGAATATATTGAGTTCTCTTGATAACATAATTGACTTATTTAATGGTGATTCATTTTTAC AGTCGCAAGAAACTGAAATAAAAATAAAGTCTCCAAGTACACTCGCAAAAATAGTGATGGATTATGGTAAAAAAGGTTTA ACTCTGCAGAGATTTAAAGGCCTTGGTGAAATGAATGCTGATCAGCTTTGGGAAACTACACTCAATCCAGAAACTAGAAC TCTGCTAAAAGTTGAAATAAAAGATTGTGAAGAAGCAGATAGCATATTCTCGGTGCTAATGGGTGATATAGTTGAACCAC GTCGTGACTTTATAAATAACAATGCACTTAACGTGCATGATGTTGATATTTGA
Upstream 100 bases:
>100_bases TTTTATCTACATGTCAAGGATCCTTCTCTCAACTTAAAGATGTTGCTAATCAGCACATACAAATGTAATATTAATTAATA TTTATAAATAAAGCAATTCC
Downstream 100 bases:
>100_bases TTTTAGAAACGCTAAACCCTTATGGCTTAGCATTGTGTGCCAAACCGGCTATTTAAAACGTACTACTAACCAATCCATTT CAGAAATCTGAATTGATCTT
Product: DNA gyrase, B subunit
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 790; Mature: 790
Protein sequence:
>790_residues MESNYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDESLAGYCSKIEVSINEDGSVSVTDNGRGIPT DIHPEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFEDGESIESLKIINEN TNKRGTRVTFMPSTGTFSSIDFSYSTLESRIRELAFLNSNIEIILRDLRNKPHVESHFNDSKESEDNFGTANFVRYLDKN KTHVTKIASIRDNVKDLGTSVEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTRCINNYANNEGFLKKAKVNLT GEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVESIVFDKLSTILETDPKLAASIVERAIRSAKGREAARKAREL VKNKNNINIATLPGKLADCQEKVPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERAGLDRIFSSAEIGSLI TAIGAGIGSEHFDIEKTRYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQPPLYKVTKNAKDTYIKDDET FEEYIVNSAIKRLTLNGVGKDLRFVLNKCLSISNISKNYDREIPKDLLESLLILSKKNALLSADEILKYLKLMYSEYNWE VEVKDEEIHIAKLFQGLADKYVFSLSMLESKEIRNILSSLDNIIDLFNGDSFLQSQETEIKIKSPSTLAKIVMDYGKKGL TLQRFKGLGEMNADQLWETTLNPETRTLLKVEIKDCEEADSIFSVLMGDIVEPRRDFINNNALNVHDVDI
Sequences:
>Translated_790_residues MESNYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDESLAGYCSKIEVSINEDGSVSVTDNGRGIPT DIHPEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFEDGESIESLKIINEN TNKRGTRVTFMPSTGTFSSIDFSYSTLESRIRELAFLNSNIEIILRDLRNKPHVESHFNDSKESEDNFGTANFVRYLDKN KTHVTKIASIRDNVKDLGTSVEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTRCINNYANNEGFLKKAKVNLT GEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVESIVFDKLSTILETDPKLAASIVERAIRSAKGREAARKAREL VKNKNNINIATLPGKLADCQEKVPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERAGLDRIFSSAEIGSLI TAIGAGIGSEHFDIEKTRYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQPPLYKVTKNAKDTYIKDDET FEEYIVNSAIKRLTLNGVGKDLRFVLNKCLSISNISKNYDREIPKDLLESLLILSKKNALLSADEILKYLKLMYSEYNWE VEVKDEEIHIAKLFQGLADKYVFSLSMLESKEIRNILSSLDNIIDLFNGDSFLQSQETEIKIKSPSTLAKIVMDYGKKGL TLQRFKGLGEMNADQLWETTLNPETRTLLKVEIKDCEEADSIFSVLMGDIVEPRRDFINNNALNVHDVDI >Mature_790_residues MESNYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDESLAGYCSKIEVSINEDGSVSVTDNGRGIPT DIHPEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFEDGESIESLKIINEN TNKRGTRVTFMPSTGTFSSIDFSYSTLESRIRELAFLNSNIEIILRDLRNKPHVESHFNDSKESEDNFGTANFVRYLDKN KTHVTKIASIRDNVKDLGTSVEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTRCINNYANNEGFLKKAKVNLT GEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVESIVFDKLSTILETDPKLAASIVERAIRSAKGREAARKAREL VKNKNNINIATLPGKLADCQEKVPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERAGLDRIFSSAEIGSLI TAIGAGIGSEHFDIEKTRYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQPPLYKVTKNAKDTYIKDDET FEEYIVNSAIKRLTLNGVGKDLRFVLNKCLSISNISKNYDREIPKDLLESLLILSKKNALLSADEILKYLKLMYSEYNWE VEVKDEEIHIAKLFQGLADKYVFSLSMLESKEIRNILSSLDNIIDLFNGDSFLQSQETEIKIKSPSTLAKIVMDYGKKGL TLQRFKGLGEMNADQLWETTLNPETRTLLKVEIKDCEEADSIFSVLMGDIVEPRRDFINNNALNVHDVDI
Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]
COG id: COG0187
COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI19913406, Length=577, Percent_Identity=24.0901213171577, Blast_Score=113, Evalue=6e-25, Organism=Homo sapiens, GI19913408, Length=578, Percent_Identity=24.5674740484429, Blast_Score=109, Evalue=1e-23, Organism=Escherichia coli, GI48994957, Length=814, Percent_Identity=50, Blast_Score=765, Evalue=0.0, Organism=Escherichia coli, GI1789408, Length=549, Percent_Identity=38.4335154826958, Blast_Score=369, Evalue=1e-103, Organism=Caenorhabditis elegans, GI17535065, Length=553, Percent_Identity=22.9656419529837, Blast_Score=108, Evalue=1e-23, Organism=Caenorhabditis elegans, GI212645845, Length=521, Percent_Identity=24.1842610364683, Blast_Score=105, Evalue=1e-22, Organism=Saccharomyces cerevisiae, GI6324241, Length=596, Percent_Identity=25, Blast_Score=116, Evalue=2e-26, Organism=Drosophila melanogaster, GI17136538, Length=567, Percent_Identity=24.8677248677249, Blast_Score=120, Evalue=3e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003594 - InterPro: IPR011557 - InterPro: IPR020568 - InterPro: IPR014721 - InterPro: IPR001241 - InterPro: IPR013759 - InterPro: IPR002288 - InterPro: IPR013506 - InterPro: IPR013760 - InterPro: IPR018522 - InterPro: IPR006171 [H]
Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]
EC number: =5.99.1.3 [H]
Molecular weight: Translated: 88678; Mature: 88678
Theoretical pI: Translated: 6.04; Mature: 6.04
Prosite motif: PS00177 TOPOISOMERASE_II
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MESNYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDESLAGYCSKIE CCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEE VSINEDGSVSVTDNGRGIPTDIHPEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGI EEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH SVVNALSSWLELTIWRNKKEHFMRFEDGESIESLKIINENTNKRGTRVTFMPSTGTFSSI HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCC DFSYSTLESRIRELAFLNSNIEIILRDLRNKPHVESHFNDSKESEDNFGTANFVRYLDKN CCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCC KTHVTKIASIRDNVKDLGTSVEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTR CHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHH CINNYANNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVES HHHHHCCCCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH IVFDKLSTILETDPKLAASIVERAIRSAKGREAARKARELVKNKNNINIATLPGKLADCQ HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHH EKVPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERAGLDRIFSSAEIGSLI HHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHH TAIGAGIGSEHFDIEKTRYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQ HHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC PPLYKVTKNAKDTYIKDDETFEEYIVNSAIKRLTLNGVGKDLRFVLNKCLSISNISKNYD CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH REIPKDLLESLLILSKKNALLSADEILKYLKLMYSEYNWEVEVKDEEIHIAKLFQGLADK HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHH YVFSLSMLESKEIRNILSSLDNIIDLFNGDSFLQSQETEIKIKSPSTLAKIVMDYGKKGL HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCHHHHHHHHHHCCCCC TLQRFKGLGEMNADQLWETTLNPETRTLLKVEIKDCEEADSIFSVLMGDIVEPRRDFINN CHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC NALNVHDVDI CCCCEEECCC >Mature Secondary Structure MESNYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDESLAGYCSKIE CCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEE VSINEDGSVSVTDNGRGIPTDIHPEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGI EEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH SVVNALSSWLELTIWRNKKEHFMRFEDGESIESLKIINENTNKRGTRVTFMPSTGTFSSI HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCC DFSYSTLESRIRELAFLNSNIEIILRDLRNKPHVESHFNDSKESEDNFGTANFVRYLDKN CCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCC KTHVTKIASIRDNVKDLGTSVEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTR CHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHH CINNYANNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVES HHHHHCCCCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH IVFDKLSTILETDPKLAASIVERAIRSAKGREAARKARELVKNKNNINIATLPGKLADCQ HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHH EKVPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERAGLDRIFSSAEIGSLI HHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHH TAIGAGIGSEHFDIEKTRYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQ HHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC PPLYKVTKNAKDTYIKDDETFEEYIVNSAIKRLTLNGVGKDLRFVLNKCLSISNISKNYD CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH REIPKDLLESLLILSKKNALLSADEILKYLKLMYSEYNWEVEVKDEEIHIAKLFQGLADK HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHH YVFSLSMLESKEIRNILSSLDNIIDLFNGDSFLQSQETEIKIKSPSTLAKIVMDYGKKGL HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCHHHHHHHHHHCCCCC TLQRFKGLGEMNADQLWETTLNPETRTLLKVEIKDCEEADSIFSVLMGDIVEPRRDFINN CHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC NALNVHDVDI CCCCEEECCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA