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The map label for this gene is gatA [H]

Identifier: 190571486

GI number: 190571486

Start: 1178156

End: 1179622

Strand: Reverse

Name: gatA [H]

Synonym: WPa_1102

Alternate gene names: 190571486

Gene position: 1179622-1178156 (Counterclockwise)

Preceding gene: 190571487

Following gene: 190571485

Centisome position: 79.57

GC content: 37.42

Gene sequence:

>1467_bases
ATGAATGAATTGAGAAGGCTCACTATTACAGAGATGCATGATGGACTTAGGAGGAGGAATTTTTCTGCTGTTGAGCTTGT
AGAAACGCATATTAATGCAGTTGAAAATGAGCAACTAAATGCATTTATAACAAAAACTCCAGAAATCGCAATAAAAGCCG
CTAAAATAGCAGATGAACATTTCTTAAAACAAAAAGATGATATTCCACCACTTATGGGCATACCAGTTGGTGTTAAAGAT
TTATTCTGTACAAAAGGAATAAAAACAACAGCATGTTCAAAAATGCTGGAAAATTTCATCCCGACTTATGAATCTACGGT
ATCTAATTTGCTTTTAAAAAGTGGAGCAGCCGTGCTTGGTAAGCTTAATATGGATGAATTTGCTATGGGTTCTGCGAACA
CAAATAGCTATTTTGGTCCTGTTGAAAATGTGTGGATTAGAAAAAGTGATGGAGCAAAAGTTGTTCCTGGTGGATCTTCC
GGTGGGTCTGCAGCATCAGTTGCTGGGTTTTTATGTGCTGGAGCATTAGGAAGCGATACTGGTGGGTCTGTACGTCAACC
AGCAGCTTATTGCGGAGTAGTGGGAATAAAGCCAACTTATGGAAGGTGTTCGCGTTTTGGTATGATTGCGTTTGCAAGTT
CTCTCGATCAAGCAGGAGTAATTACTCGTTCCGTTTCTGATTCAGCATTAATGCTTGAAATAATTTGTGGCTATGATGAA
AAAGATTCAACATCGAGCAAAAGGACAGTGCCTAAATTTTCCAGTTTTATAAATGGTGATATTAAGGGTAAGCGCATTGG
TATACCAAAAGAGTATAAGATGGATGGAATTTCAGATGAAATAGTTCATAATTGGGAAAAAGTTTCTTCCAATTTAAAAG
AAAATGGTGCTGAAGTTGTTGACATTACTCTGCCTCATACTAAATATGCGATACCGGTTTACTATCTAATTTGTTCTGCT
GAAACTTCATCTAATCTTGCTCGCTATGATGGTGTACGTTATGGATTCAGGGTTGATGCTGATACCCTTGAAGAAATGTA
TTCGCTAACAAGAGCAGAAGGTTTTGGTAAAGAAGTGAAAAGGAGAATTTTAATTGGTTCTTATGCTCTTTCTTCAGGTC
ATTACAATGAGTATTATGAAAAAGCACAGTGCATTAGGGCATTAATCAGAAATGATTTTATAAAAGCGTTTGAAAAAATA
GATTACATACTTGTGCCATCTGCTCCAACAGAGGCTTTTGGCTTAAATGAAAAGCCAGACCCGCTAATTATGTGCATCAA
TGATGTGTTTACCGTGCCGGCAAGCTTAGCAGGGTTACCTGCTATTTCTGTTCCTGTTGGACTTTCCAATGCAGGCTTAC
CACTTGCTTTACAAGTAATTGGAAATTATTACGATGAAGCTGGAATATTAAATATGGCAAGTGTGATAGAGCAAAATTGT
GGCAGAATAGTTAATTCCCTTACGTAA

Upstream 100 bases:

>100_bases
TCTGAATTGATCTTGATTGTATTCCCGTTTTCGATCATAAATATATAGATTTTCTGTCCTATTGTTGTTATAATTAATGC
CTCGCTAAAATTGAATATTA

Downstream 100 bases:

>100_bases
TATGTTTTCTTATAGTAACTATAGCATTATGATTATTTTGGCTATAGGAAACAAGAGCTTGATTTTTACAGCTAAATAAC
AGACCATCAAAGGCACACAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MNELRRLTITEMHDGLRRRNFSAVELVETHINAVENEQLNAFITKTPEIAIKAAKIADEHFLKQKDDIPPLMGIPVGVKD
LFCTKGIKTTACSKMLENFIPTYESTVSNLLLKSGAAVLGKLNMDEFAMGSANTNSYFGPVENVWIRKSDGAKVVPGGSS
GGSAASVAGFLCAGALGSDTGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEIICGYDE
KDSTSSKRTVPKFSSFINGDIKGKRIGIPKEYKMDGISDEIVHNWEKVSSNLKENGAEVVDITLPHTKYAIPVYYLICSA
ETSSNLARYDGVRYGFRVDADTLEEMYSLTRAEGFGKEVKRRILIGSYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKI
DYILVPSAPTEAFGLNEKPDPLIMCINDVFTVPASLAGLPAISVPVGLSNAGLPLALQVIGNYYDEAGILNMASVIEQNC
GRIVNSLT

Sequences:

>Translated_488_residues
MNELRRLTITEMHDGLRRRNFSAVELVETHINAVENEQLNAFITKTPEIAIKAAKIADEHFLKQKDDIPPLMGIPVGVKD
LFCTKGIKTTACSKMLENFIPTYESTVSNLLLKSGAAVLGKLNMDEFAMGSANTNSYFGPVENVWIRKSDGAKVVPGGSS
GGSAASVAGFLCAGALGSDTGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEIICGYDE
KDSTSSKRTVPKFSSFINGDIKGKRIGIPKEYKMDGISDEIVHNWEKVSSNLKENGAEVVDITLPHTKYAIPVYYLICSA
ETSSNLARYDGVRYGFRVDADTLEEMYSLTRAEGFGKEVKRRILIGSYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKI
DYILVPSAPTEAFGLNEKPDPLIMCINDVFTVPASLAGLPAISVPVGLSNAGLPLALQVIGNYYDEAGILNMASVIEQNC
GRIVNSLT
>Mature_488_residues
MNELRRLTITEMHDGLRRRNFSAVELVETHINAVENEQLNAFITKTPEIAIKAAKIADEHFLKQKDDIPPLMGIPVGVKD
LFCTKGIKTTACSKMLENFIPTYESTVSNLLLKSGAAVLGKLNMDEFAMGSANTNSYFGPVENVWIRKSDGAKVVPGGSS
GGSAASVAGFLCAGALGSDTGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEIICGYDE
KDSTSSKRTVPKFSSFINGDIKGKRIGIPKEYKMDGISDEIVHNWEKVSSNLKENGAEVVDITLPHTKYAIPVYYLICSA
ETSSNLARYDGVRYGFRVDADTLEEMYSLTRAEGFGKEVKRRILIGSYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKI
DYILVPSAPTEAFGLNEKPDPLIMCINDVFTVPASLAGLPAISVPVGLSNAGLPLALQVIGNYYDEAGILNMASVIEQNC
GRIVNSLT

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=41.7004048582996, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI195972892, Length=501, Percent_Identity=25.5489021956088, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=36.4628820960699, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=511, Percent_Identity=25.2446183953033, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=299, Percent_Identity=26.7558528428094, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=296, Percent_Identity=27.7027027027027, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=324, Percent_Identity=26.8518518518519, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=324, Percent_Identity=26.8518518518519, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=37.2960372960373, Blast_Score=242, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=423, Percent_Identity=27.4231678486998, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648113, Length=462, Percent_Identity=43.2900432900433, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=24.6963562753036, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=24.6963562753036, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=24.6963562753036, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=24.6963562753036, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644968, Length=256, Percent_Identity=31.640625, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161078093, Length=524, Percent_Identity=24.236641221374, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=23.3128834355828, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=30.7291666666667, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=30.7291666666667, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=30.7291666666667, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648441, Length=439, Percent_Identity=23.0068337129841, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648439, Length=439, Percent_Identity=23.0068337129841, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 52894; Mature: 52894

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRRLTITEMHDGLRRRNFSAVELVETHINAVENEQLNAFITKTPEIAIKAAKIADEH
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHH
FLKQKDDIPPLMGIPVGVKDLFCTKGIKTTACSKMLENFIPTYESTVSNLLLKSGAAVLG
HHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEE
KLNMDEFAMGSANTNSYFGPVENVWIRKSDGAKVVPGGSSGGSAASVAGFLCAGALGSDT
CCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCC
GGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEIICGYDE
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEHEEECCCC
KDSTSSKRTVPKFSSFINGDIKGKRIGIPKEYKMDGISDEIVHNWEKVSSNLKENGAEVV
CCCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DITLPHTKYAIPVYYLICSAETSSNLARYDGVRYGFRVDADTLEEMYSLTRAEGFGKEVK
EEECCCCCCEEEEEEEEEECCCCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHCCCHHHH
RRILIGSYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKIDYILVPSAPTEAFGLNEKPD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHCCCCCCCC
PLIMCINDVFTVPASLAGLPAISVPVGLSNAGLPLALQVIGNYYDEAGILNMASVIEQNC
CEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
GRIVNSLT
HHHHHCCC
>Mature Secondary Structure
MNELRRLTITEMHDGLRRRNFSAVELVETHINAVENEQLNAFITKTPEIAIKAAKIADEH
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHH
FLKQKDDIPPLMGIPVGVKDLFCTKGIKTTACSKMLENFIPTYESTVSNLLLKSGAAVLG
HHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEE
KLNMDEFAMGSANTNSYFGPVENVWIRKSDGAKVVPGGSSGGSAASVAGFLCAGALGSDT
CCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCC
GGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEIICGYDE
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEHEEECCCC
KDSTSSKRTVPKFSSFINGDIKGKRIGIPKEYKMDGISDEIVHNWEKVSSNLKENGAEVV
CCCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DITLPHTKYAIPVYYLICSAETSSNLARYDGVRYGFRVDADTLEEMYSLTRAEGFGKEVK
EEECCCCCCEEEEEEEEEECCCCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHCCCHHHH
RRILIGSYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKIDYILVPSAPTEAFGLNEKPD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHCCCCCCCC
PLIMCINDVFTVPASLAGLPAISVPVGLSNAGLPLALQVIGNYYDEAGILNMASVIEQNC
CEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
GRIVNSLT
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA