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The map label for this gene is pmbA [H]

Identifier: 190571461

GI number: 190571461

Start: 1145406

End: 1146740

Strand: Direct

Name: pmbA [H]

Synonym: WPa_1076

Alternate gene names: 190571461

Gene position: 1145406-1146740 (Clockwise)

Preceding gene: 190571460

Following gene: 190571462

Centisome position: 77.26

GC content: 33.03

Gene sequence:

>1335_bases
ATGAATATATTAAATATTGCAGCAGATATCACTAAACTAATAAAAAAGCAGAATCAAGACGCAGAAGTTACAATATATGA
AACTAATAAGACCTCGGTTTCTCAGCGTCTATCAAAAATTGAACAAATATCACAATCTAAAAACTGTACTGTCGGAATTA
GAGCTATAGCAGGTAAGAACAAAGCTGCGTATATTTCCACAAACGATTTGAATAATCTTAGTAGTGCAGTGAGCCAAGTG
GTGGAAATGGCAAAGAATGCCCAGGAAGATCCTTATATTAGTTTTGCTGTAGATGGCAGTAATTATATCTCTTCTGCAGA
TTTAAATATCTCAGATAATAATGTTGTAACAATTGATAACTTAAAAGAAATTACTGAAGCTACAGAAAATTCAGCTCTTG
CACATAAAAATATTATTAATTCCGAAGGAGCTTCATCTTCACATACTTCAGTAAATACAGTATTATCAACCGTTTCTGGT
TTTGTTGGTTCATTTAGTAAGTCAACTTTTGTTAATCAGGTTTCTGTTGTTGCTGGAGAAAAGAATGAAATGAAAATAGG
CTACGATTACGATGTAGCATGCAACTTCAGTGATTTAAAAACACCGGAGTCAATAGGGAAAGAAGCAGCAAAAAGAGCAA
TAGATCAATTGGGTTCATGTACAATCAGGACAGGCAAATTTCCGGTTGTTTTTGAAAAAAGAGCAGCAAAAGAGCTAGTA
AAGAGCTTTGCTTCTGCCATAAATGGTAGCAATATTGTAAATAATAGCTCTTTTTTGAGAAATAGTTTAAATAATCAAAT
TTTTAATACTGAGATTAGCATTATTGATGACCCATTGTTACCAAGGGGTATAACATCAAGACCGTTTGACGGGGAAGGAA
TAATGAGCAGAAGAAATGAATTTGTAAAAAATGGAGTACTGCAAAACTGGATTTTAGATCTATATTCGGCTAGGAAATTA
AACTTGGAAACGACCGCAAATGCAACTCGTGCAAGTAATGCTGCAATTATTCCTTCAGCTAGTAATTTCTATTTCAAAAA
TGGTAATAGATCGTTTAAGGAATTAATTGAGGAAGTAAAAGAAGGAATATATGTAACTGATTTATTTGGTTTTGGTGTTA
ATTTAATCAATGGCGATTATAGTCAAGGTGCATGCGGATTTTTTATAGAAAATGGTAAAATAACGTATCCAATACACGAG
ATTACTGTTGCCGACAATTTGAAAAATATGTTTAGCAATTTAGTTGTTGCAAATGACCTAACTTTCTGTGGGCAGTTTAA
TTCACCAACGATTAAAGTTAGCGAAATGACAGTAGCAGGTTCACTTAGTGATTAA

Upstream 100 bases:

>100_bases
AGCATTACTTTATAATCGTTCTCGTCAAGCAGCAATTACAACTGATTTGATTGAAGTTATAGGTGGTGCAGAATCTTTAT
AGAAACTCAAGGAATGAAAA

Downstream 100 bases:

>100_bases
AAATTTTAGTTGCATTAAGTACAATAATTGTGTACTATTACTATGTAATGTTAAGAATAGACTATGGAGTATAGAAGTTT
AGTTGCAGTAACTAAGCTTA

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKNKAAYISTNDLNNLSSAVSQV
VEMAKNAQEDPYISFAVDGSNYISSADLNISDNNVVTIDNLKEITEATENSALAHKNIINSEGASSSHTSVNTVLSTVSG
FVGSFSKSTFVNQVSVVAGEKNEMKIGYDYDVACNFSDLKTPESIGKEAAKRAIDQLGSCTIRTGKFPVVFEKRAAKELV
KSFASAINGSNIVNNSSFLRNSLNNQIFNTEISIIDDPLLPRGITSRPFDGEGIMSRRNEFVKNGVLQNWILDLYSARKL
NLETTANATRASNAAIIPSASNFYFKNGNRSFKELIEEVKEGIYVTDLFGFGVNLINGDYSQGACGFFIENGKITYPIHE
ITVADNLKNMFSNLVVANDLTFCGQFNSPTIKVSEMTVAGSLSD

Sequences:

>Translated_444_residues
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKNKAAYISTNDLNNLSSAVSQV
VEMAKNAQEDPYISFAVDGSNYISSADLNISDNNVVTIDNLKEITEATENSALAHKNIINSEGASSSHTSVNTVLSTVSG
FVGSFSKSTFVNQVSVVAGEKNEMKIGYDYDVACNFSDLKTPESIGKEAAKRAIDQLGSCTIRTGKFPVVFEKRAAKELV
KSFASAINGSNIVNNSSFLRNSLNNQIFNTEISIIDDPLLPRGITSRPFDGEGIMSRRNEFVKNGVLQNWILDLYSARKL
NLETTANATRASNAAIIPSASNFYFKNGNRSFKELIEEVKEGIYVTDLFGFGVNLINGDYSQGACGFFIENGKITYPIHE
ITVADNLKNMFSNLVVANDLTFCGQFNSPTIKVSEMTVAGSLSD
>Mature_444_residues
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKNKAAYISTNDLNNLSSAVSQV
VEMAKNAQEDPYISFAVDGSNYISSADLNISDNNVVTIDNLKEITEATENSALAHKNIINSEGASSSHTSVNTVLSTVSG
FVGSFSKSTFVNQVSVVAGEKNEMKIGYDYDVACNFSDLKTPESIGKEAAKRAIDQLGSCTIRTGKFPVVFEKRAAKELV
KSFASAINGSNIVNNSSFLRNSLNNQIFNTEISIIDDPLLPRGITSRPFDGEGIMSRRNEFVKNGVLQNWILDLYSARKL
NLETTANATRASNAAIIPSASNFYFKNGNRSFKELIEEVKEGIYVTDLFGFGVNLINGDYSQGACGFFIENGKITYPIHE
ITVADNLKNMFSNLVVANDLTFCGQFNSPTIKVSEMTVAGSLSD

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=434, Percent_Identity=32.7188940092166, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789640, Length=257, Percent_Identity=25.6809338521401, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48282; Mature: 48282

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKN
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
KAAYISTNDLNNLSSAVSQVVEMAKNAQEDPYISFAVDGSNYISSADLNISDNNVVTIDN
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCCCCEEEECC
LKEITEATENSALAHKNIINSEGASSSHTSVNTVLSTVSGFVGSFSKSTFVNQVSVVAGE
HHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECC
KNEMKIGYDYDVACNFSDLKTPESIGKEAAKRAIDQLGSCTIRTGKFPVVFEKRAAKELV
CCCEEECCCCEEEECCHHCCCHHHHHHHHHHHHHHHHCCEEEECCCCCEEECHHHHHHHH
KSFASAINGSNIVNNSSFLRNSLNNQIFNTEISIIDDPLLPRGITSRPFDGEGIMSRRNE
HHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
FVKNGVLQNWILDLYSARKLNLETTANATRASNAAIIPSASNFYFKNGNRSFKELIEEVK
HHHHHHHHHHHHHHHHCCEECEEECCCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHH
EGIYVTDLFGFGVNLINGDYSQGACGFFIENGKITYPIHEITVADNLKNMFSNLVVANDL
CCEEEEEHHHCCEEEECCCCCCCCEEEEEECCEEEEEEEEEEHHHHHHHHHHHEEEECCC
TFCGQFNSPTIKVSEMTVAGSLSD
EEECCCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure
MNILNIAADITKLIKKQNQDAEVTIYETNKTSVSQRLSKIEQISQSKNCTVGIRAIAGKN
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
KAAYISTNDLNNLSSAVSQVVEMAKNAQEDPYISFAVDGSNYISSADLNISDNNVVTIDN
CEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCCCCEEEECC
LKEITEATENSALAHKNIINSEGASSSHTSVNTVLSTVSGFVGSFSKSTFVNQVSVVAGE
HHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECC
KNEMKIGYDYDVACNFSDLKTPESIGKEAAKRAIDQLGSCTIRTGKFPVVFEKRAAKELV
CCCEEECCCCEEEECCHHCCCHHHHHHHHHHHHHHHHCCEEEECCCCCEEECHHHHHHHH
KSFASAINGSNIVNNSSFLRNSLNNQIFNTEISIIDDPLLPRGITSRPFDGEGIMSRRNE
HHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
FVKNGVLQNWILDLYSARKLNLETTANATRASNAAIIPSASNFYFKNGNRSFKELIEEVK
HHHHHHHHHHHHHHHHCCEECEEECCCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHH
EGIYVTDLFGFGVNLINGDYSQGACGFFIENGKITYPIHEITVADNLKNMFSNLVVANDL
CCEEEEEHHHCCEEEECCCCCCCCEEEEEECCEEEEEEEEEEHHHHHHHHHHHEEEECCC
TFCGQFNSPTIKVSEMTVAGSLSD
EEECCCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]