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The map label for this gene is cspD [C]

Identifier: 190571462

GI number: 190571462

Start: 1146915

End: 1147166

Strand: Direct

Name: cspD [C]

Synonym: WPa_1077

Alternate gene names: 190571462

Gene position: 1146915-1147166 (Clockwise)

Preceding gene: 190571461

Following gene: 190571463

Centisome position: 77.37

GC content: 35.71

Gene sequence:

>252_bases
ATGGAATTTGGTAATATAAAATGGTTTAATGTTGAAAAAGGCTATGGTTTCATCAAGCCAGAAGGTAAAGGTGAGGACGT
TTTTGTACATGTTAGTACATTAGAACGTTCAGGAATAAGACCTGATGAACTTCGAGGAGAGGATAAAAAGAGAGGAATGA
AAGGGGAAAGAGTAAGTTATGAGCTTAAAGAAGAGCGTGGTAGGAACGGAGAAGATAAAAAGTCTGCAATAAATTTAAGA
TTGGAAGATTAG

Upstream 100 bases:

>100_bases
AAGTTTAGTTGCAGTAACTAAGCTTAAAGCATTCTAAATCGTAATTTCAAAGTTTTTCATCACATTAATAATGTTAGTTA
GTAGTTTAGGAGTTATCCAT

Downstream 100 bases:

>100_bases
TCTATCGTGAAGCTTTTTAATAGGGTAATGTATTGTGAGTAATTTTCATGAAATGGGGCTTCCAACTTTGCTTAGGCAAG
CTCTAAGTAAAAATAATCTT

Product: cold-shock domain family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 83; Mature: 83

Protein sequence:

>83_residues
MEFGNIKWFNVEKGYGFIKPEGKGEDVFVHVSTLERSGIRPDELRGEDKKRGMKGERVSYELKEERGRNGEDKKSAINLR
LED

Sequences:

>Translated_83_residues
MEFGNIKWFNVEKGYGFIKPEGKGEDVFVHVSTLERSGIRPDELRGEDKKRGMKGERVSYELKEERGRNGEDKKSAINLR
LED
>Mature_83_residues
MEFGNIKWFNVEKGYGFIKPEGKGEDVFVHVSTLERSGIRPDELRGEDKKRGMKGERVSYELKEERGRNGEDKKSAINLR
LED

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 9617; Mature: 9617

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFGNIKWFNVEKGYGFIKPEGKGEDVFVHVSTLERSGIRPDELRGEDKKRGMKGERVSY
CCCCCEEEEEECCCCEEECCCCCCCEEEEEEEECCCCCCCCHHCCCCHHHCCCCCCCEEE
ELKEERGRNGEDKKSAINLRLED
EEHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MEFGNIKWFNVEKGYGFIKPEGKGEDVFVHVSTLERSGIRPDELRGEDKKRGMKGERVSY
CCCCCEEEEEECCCCEEECCCCCCCEEEEEEEECCCCCCCCHHCCCCHHHCCCCCCCEEE
ELKEERGRNGEDKKSAINLRLED
EEHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]