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The map label for this gene is trmE

Identifier: 190571401

GI number: 190571401

Start: 1075557

End: 1076885

Strand: Direct

Name: trmE

Synonym: WPa_1009

Alternate gene names: 190571401

Gene position: 1075557-1076885 (Clockwise)

Preceding gene: 190571396

Following gene: 190571402

Centisome position: 72.55

GC content: 35.52

Gene sequence:

>1329_bases
ATGACAAACACAAATGAAACTATTTTTGCTTTGTCGACCGTATTTGGTAAGTCAGGAGTTGCAGTAATCAGAATTTCAGG
CAACTACACCCTTAAAGCTTTAAATCATTTTCATATTAAGAAAAAAATCAAACCAAGATTTGCTACTTTAGTTGATCTAT
ATGATGATTCCAATCAATTGATAGATAATGGAATAATCATCTATTTCCCTGCTCCAAACAGTTTCACTGGCGAGGATGTT
ATAGAGTTACAAGTGCATGGAAGCAAGGCCGTCATAAAAATCATCTTAGAGAAATTATCAAAAATTTTCGTTATGGCCAG
GCCTGGAGAATTCTCACTTAGGGCTTTTCTAAATGGTAAATTTGACTTAACGCAAATAGAAGGAATTGCAGACTTAATTG
ATGCTGAAACGAAAATGCAAGCTAAACAAGCGATTAAGCAGATATCAGGAGAATTGGAGAGACTATACAGCAATTGGAGG
CAAAGATTAATAACGATACAATCCAAAATCGAAGCATATATAGACTTTCCAGAGGACATTTGGGCAGAAAAAAGTGAATT
GGAAAAAATTAATAATGAAGTGCAATCTCTCGTGCAGTTGATACAAGAGCATTTAAATGATAATAGACGGGGCGAAAGGT
TGCGTGAGGGTTTACATATTGTAATAACTGGTGAACCAAATGTCGGTAAATCAACTCTGTTTAATTTCTTAGCCAAGCGT
GATATTGCTATTGTTTCTGAATATGCAGGCACAACAAGAGATATACTTGAAGCTCATATTGACATTGGGGGATACCCAAT
CATTCTCTCTGATACTGCTGGAATTCGTGAGAGTTCAGATCCAATAGAATCAGAAGGTATAAGTCGAGCGAAAAAAAGGT
CTTTTGAAGCTGATTTAAGAATAGAACTATTTCCTTTTGAACAGCGTCATAATATCAATTGCAACGTTGTAAATAGCGAT
ACTATTTATGTATTGAGCAAAGCTGACGATGCAATTAATGACAGAAATATACTGATTGGCGGTGTAGATTTTCTACCTAT
TTCTATTTTAAAGGGAATAGGTACAAACAAGTTGATCTCTCTCATAAAAAAGAAGGCAGAGGAAAAATTTGGGCATGATA
GAGACACTCCTGTGATTACTCGGCAAAGACATAGGAGTCACATGCAGAAAGCACTGGAACATTTACAACGTTTTAAAATC
GATAATCCAATTGAGTTGATATCTGAAGACTTGAGGCTTGCTGCATTTGAACTTGGTGCAGTGATTGGAATTATTAATGT
TGAGGAAATATTGGATAGTGTGTTTAGTAGCTTTTGTGTAGGCAAGTAA

Upstream 100 bases:

>100_bases
TATTTACTAATACTGCATTAATATGAATAAGTAATATAAGCAAAAATTTTTTAGATTAGGTATTTCGATTTCTTAAATTG
CTAAGTAGTTTATATAAATA

Downstream 100 bases:

>100_bases
GATTTTTATAGATTGCTTGAAATTAAATATTGTGGTAAAAAAGAGGTGGACGGATGGCCGAGCGGTTTAAGGCACCAGTC
TTGAAAACTGACGTACGGAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTNTNETIFALSTVFGKSGVAVIRISGNYTLKALNHFHIKKKIKPRFATLVDLYDDSNQLIDNGIIIYFPAPNSFTGEDV
IELQVHGSKAVIKIILEKLSKIFVMARPGEFSLRAFLNGKFDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWR
QRLITIQSKIEAYIDFPEDIWAEKSELEKINNEVQSLVQLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIESEGISRAKKRSFEADLRIELFPFEQRHNINCNVVNSD
TIYVLSKADDAINDRNILIGGVDFLPISILKGIGTNKLISLIKKKAEEKFGHDRDTPVITRQRHRSHMQKALEHLQRFKI
DNPIELISEDLRLAAFELGAVIGIINVEEILDSVFSSFCVGK

Sequences:

>Translated_442_residues
MTNTNETIFALSTVFGKSGVAVIRISGNYTLKALNHFHIKKKIKPRFATLVDLYDDSNQLIDNGIIIYFPAPNSFTGEDV
IELQVHGSKAVIKIILEKLSKIFVMARPGEFSLRAFLNGKFDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWR
QRLITIQSKIEAYIDFPEDIWAEKSELEKINNEVQSLVQLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIESEGISRAKKRSFEADLRIELFPFEQRHNINCNVVNSD
TIYVLSKADDAINDRNILIGGVDFLPISILKGIGTNKLISLIKKKAEEKFGHDRDTPVITRQRHRSHMQKALEHLQRFKI
DNPIELISEDLRLAAFELGAVIGIINVEEILDSVFSSFCVGK
>Mature_441_residues
TNTNETIFALSTVFGKSGVAVIRISGNYTLKALNHFHIKKKIKPRFATLVDLYDDSNQLIDNGIIIYFPAPNSFTGEDVI
ELQVHGSKAVIKIILEKLSKIFVMARPGEFSLRAFLNGKFDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWRQ
RLITIQSKIEAYIDFPEDIWAEKSELEKINNEVQSLVQLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKRD
IAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIESEGISRAKKRSFEADLRIELFPFEQRHNINCNVVNSDT
IYVLSKADDAINDRNILIGGVDFLPISILKGIGTNKLISLIKKKAEEKFGHDRDTPVITRQRHRSHMQKALEHLQRFKID
NPIELISEDLRLAAFELGAVIGIINVEEILDSVFSSFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=462, Percent_Identity=38.961038961039, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI193082993, Length=449, Percent_Identity=39.1982182628062, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=36.4372469635627, Blast_Score=313, Evalue=1e-85,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=36.304347826087, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17507259, Length=443, Percent_Identity=32.2799097065463, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=35.9918200408998, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=39.2857142857143, Blast_Score=311, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_WOLPP (B3CMJ7)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975759.1
- EnsemblBacteria:   EBWOLT00000000960
- GeneID:   6385048
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_1009
- GeneTree:   EBGT00050000031262
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49839; Mature: 49708

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 82-82 BINDING 120-120 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTNETIFALSTVFGKSGVAVIRISGNYTLKALNHFHIKKKIKPRFATLVDLYDDSNQL
CCCCCCEEEEEEHHHCCCCEEEEEECCCEEEEEHHHHHHHHHCCHHHHHHHHEECCCCCE
IDNGIIIYFPAPNSFTGEDVIELQVHGSKAVIKIILEKLSKIFVMARPGEFSLRAFLNGK
ECCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECCC
FDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWRQRLITIQSKIEAYIDFPEDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WAEKSELEKINNEVQSLVQLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIESEGISRAKKRSFEADLR
CHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
IELFPFEQRHNINCNVVNSDTIYVLSKADDAINDRNILIGGVDFLPISILKGIGTNKLIS
EEEEECCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHCCCHHHHHH
LIKKKAEEKFGHDRDTPVITRQRHRSHMQKALEHLQRFKIDNPIELISEDLRLAAFELGA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIGIINVEEILDSVFSSFCVGK
HHEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNTNETIFALSTVFGKSGVAVIRISGNYTLKALNHFHIKKKIKPRFATLVDLYDDSNQL
CCCCCEEEEEEHHHCCCCEEEEEECCCEEEEEHHHHHHHHHCCHHHHHHHHEECCCCCE
IDNGIIIYFPAPNSFTGEDVIELQVHGSKAVIKIILEKLSKIFVMARPGEFSLRAFLNGK
ECCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECCC
FDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWRQRLITIQSKIEAYIDFPEDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WAEKSELEKINNEVQSLVQLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIESEGISRAKKRSFEADLR
CHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
IELFPFEQRHNINCNVVNSDTIYVLSKADDAINDRNILIGGVDFLPISILKGIGTNKLIS
EEEEECCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHCCCHHHHHH
LIKKKAEEKFGHDRDTPVITRQRHRSHMQKALEHLQRFKIDNPIELISEDLRLAAFELGA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIGIINVEEILDSVFSSFCVGK
HHEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA