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The map label for this gene is nuoN [H]

Identifier: 190571389

GI number: 190571389

Start: 1065010

End: 1066395

Strand: Direct

Name: nuoN [H]

Synonym: WPa_0997

Alternate gene names: 190571389

Gene position: 1065010-1066395 (Clockwise)

Preceding gene: 190571388

Following gene: 190571390

Centisome position: 71.84

GC content: 33.77

Gene sequence:

>1386_bases
ATGAATTATATACAGATATTGCCGGAAATGTTCTCTATTATCTCCTCGTTAGTGTTGCTACTGCTTGGGATTATATTTAA
CCGCAGAACTATCAACTTATTAGCACTTGGCTGCACAGTGGTAACTTTAATTATTTTAATTCTTTCGGCAGAAAACAATG
AAATTTTTCTCTTTAATTCGTTGTTAAAACTCAATTTATACATCAGGTCAGCCCAAGGGTTAATTCTCAGCACAGGAATT
TTAATACTTTTGATGCTGAATTTATCAAAATATGACTATAAATATGAATTTTCAATACTGATTCTTTTTGCACTGTTTGG
CATGATAACTTTGGTTTCAGCAAATAGTCTGATCTCTTTTTATTTAGCTTTTGAGTTGATGAGTATATCTTTGTATGTTC
TTGCGAGCTTTAATAAAGATTCGATTTATTCATGTGAAGCGGGAGTGAAATATTTTACACTCAGTGCACTATCTTCCTGC
ATTATGCTATATGGAATGTCGCTGCTTTATGGATATACAGGACAAGTCAATTTCTCTGAGCTCGGTTCATTCTTGCAAAA
CCATCAGATAACTTATGGAATAGTCTTTGGGTTAGTCTTTGTTCTAATTGGTTTGTGTTTCAAGCTTGCTATTGTTCCTT
TTCATATGTGGGCTCCAGATGTTTATCAAGGTGCACCTACCATAGTAACTGCGTTTTTTTCTACGGCCCCAAAAGCTGCA
CTTGTAACATTTTTAATTCGATTGATGAATGAAGAGTTAGTAAATGTAAAAAGTTATGTTCAGCCTATTTTCTTATACGT
TTCAGCATTGTCTGTGCTTATCTCAGCTTTTGGGGCTTTGCGTCAGCAAAACTTAAAAAGGCTGCTTGCTTACAGTTCAA
TTGGTCACATTGGTTTTATATTTGCTTCACTTTCTATTTTTACACAAGCAGGAACAGATAGTGCCTTAATGTATCTGGTG
ATATATATCATCACAAGCATAGGGCTATTCTCATATCTCGTACAAATTGACGATGATGATTGTGATATTGCAAATTTATC
TGGTATAGGAAAAAAGCAACCAATTTTAGCATTCCATCTTTCTATACTATTACTCTCGATGTCGGGAATACCTCCACTTG
CAGGTTTTATCGCTAAATTTTTTATATTCAAAAGTTTAATAAATTCTGGCTTTATCAGCCTGTCTTTGATCCTTGTAATA
TCGAGTGTGATATCATGCTACTATTATTTAAATATCATGAAAGTTATGTATTTTGATAAAGCTAGTGGTAATAAGGTTGC
TTATTCTAAGAGTCTATTCATTATCACTTCAGTGGCTTCACTGATCAATCTCGTTCTTTTCTTGTATGTAGAAGATCTTG
ACTCACTCATTAAACTAAAGGGCTGA

Upstream 100 bases:

>100_bases
TGCTTGCAGTGTTTGTTATTTTGCTTGGATTTTACCCAACCCTTGCACTGAATTATTTGAAGCCATGTATGGCAAATTTG
TTAGTCAAATATAATGCGCT

Downstream 100 bases:

>100_bases
CAAGGTGATCCTTGAAGGTTTTCGTATTTATCATTACAAAGAAGTTCCAAGTACTAATAAAGAAGCATTGGGTTTAATTG
AGAAAGGGATATCAAATGAA

Product: NADH dehydrogenase I, N subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNYIQILPEMFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILILSAENNEIFLFNSLLKLNLYIRSAQGLILSTGI
LILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISFYLAFELMSISLYVLASFNKDSIYSCEAGVKYFTLSALSSC
IMLYGMSLLYGYTGQVNFSELGSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFIFASLSIFTQAGTDSALMYLV
IYIITSIGLFSYLVQIDDDDCDIANLSGIGKKQPILAFHLSILLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVI
SSVISCYYYLNIMKVMYFDKASGNKVAYSKSLFIITSVASLINLVLFLYVEDLDSLIKLKG

Sequences:

>Translated_461_residues
MNYIQILPEMFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILILSAENNEIFLFNSLLKLNLYIRSAQGLILSTGI
LILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISFYLAFELMSISLYVLASFNKDSIYSCEAGVKYFTLSALSSC
IMLYGMSLLYGYTGQVNFSELGSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFIFASLSIFTQAGTDSALMYLV
IYIITSIGLFSYLVQIDDDDCDIANLSGIGKKQPILAFHLSILLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVI
SSVISCYYYLNIMKVMYFDKASGNKVAYSKSLFIITSVASLINLVLFLYVEDLDSLIKLKG
>Mature_461_residues
MNYIQILPEMFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILILSAENNEIFLFNSLLKLNLYIRSAQGLILSTGI
LILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISFYLAFELMSISLYVLASFNKDSIYSCEAGVKYFTLSALSSC
IMLYGMSLLYGYTGQVNFSELGSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFIFASLSIFTQAGTDSALMYLV
IYIITSIGLFSYLVQIDDDDCDIANLSGIGKKQPILAFHLSILLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVI
SSVISCYYYLNIMKVMYFDKASGNKVAYSKSLFIITSVASLINLVLFLYVEDLDSLIKLKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=276, Percent_Identity=26.8115942028986, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI145693160, Length=384, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788613, Length=370, Percent_Identity=25.1351351351351, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788614, Length=325, Percent_Identity=24.3076923076923, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788831, Length=291, Percent_Identity=27.1477663230241, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788827, Length=296, Percent_Identity=23.6486486486486, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI2367154, Length=302, Percent_Identity=26.158940397351, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788829, Length=287, Percent_Identity=24.7386759581882, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51137; Mature: 51137

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIQILPEMFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILILSAENNEIFLFNS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEH
LLKLNLYIRSAQGLILSTGILILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISF
HHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAFELMSISLYVLASFNKDSIYSCEAGVKYFTLSALSSCIMLYGMSLLYGYTGQVNFSE
HHHHHHHHHHHHHEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LGSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCHHH
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FASLSIFTQAGTDSALMYLVIYIITSIGLFSYLVQIDDDDCDIANLSGIGKKQPILAFHL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHH
SILLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVISSVISCYYYLNIMKVMYFDK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
ASGNKVAYSKSLFIITSVASLINLVLFLYVEDLDSLIKLKG
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYIQILPEMFSIISSLVLLLLGIIFNRRTINLLALGCTVVTLIILILSAENNEIFLFNS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEH
LLKLNLYIRSAQGLILSTGILILLMLNLSKYDYKYEFSILILFALFGMITLVSANSLISF
HHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAFELMSISLYVLASFNKDSIYSCEAGVKYFTLSALSSCIMLYGMSLLYGYTGQVNFSE
HHHHHHHHHHHHHEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LGSFLQNHQITYGIVFGLVFVLIGLCFKLAIVPFHMWAPDVYQGAPTIVTAFFSTAPKAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHCCCHHH
LVTFLIRLMNEELVNVKSYVQPIFLYVSALSVLISAFGALRQQNLKRLLAYSSIGHIGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FASLSIFTQAGTDSALMYLVIYIITSIGLFSYLVQIDDDDCDIANLSGIGKKQPILAFHL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHH
SILLLSMSGIPPLAGFIAKFFIFKSLINSGFISLSLILVISSVISCYYYLNIMKVMYFDK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
ASGNKVAYSKSLFIITSVASLINLVLFLYVEDLDSLIKLKG
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA