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The map label for this gene is nuoM [H]

Identifier: 190571388

GI number: 190571388

Start: 1063571

End: 1065013

Strand: Direct

Name: nuoM [H]

Synonym: WPa_0996

Alternate gene names: 190571388

Gene position: 1063571-1065013 (Clockwise)

Preceding gene: 190571387

Following gene: 190571389

Centisome position: 71.74

GC content: 34.86

Gene sequence:

>1443_bases
GTGTTGTTACTTAGTATATTCTTGCTTCCACTGATAGGAGCGTTGATTTTATCCTCGATCAGGATTAATCATCAATCTAT
ACACCTAAAATTCCTTGCCCTATTTTTTGCTATACTTCCATTTTTGCTTAGCATTGTAGCTTGTATAGAATTTGATTATA
ACAATGCGGACTTTCAGTTTGTCAGCTATCCAATCAAGAATGTAGGAATAGGGATAGATGGTATATCGTTGCTTTTCCTT
CTGCTTACAACTTTCTTGTTTGTAATTTGTATACTCTATAACTGCAAAATGAGTTATACGACCCTAAAAGCCTATATGGC
ATTATTTCTACTGCTTGAGAGTTTTGTAGTCGGTTTTTTCGTTTCACTGAATGCTATAAGCTTTTATGTGTTTTTTGAAG
CTGTTTTAATACCAATGTTCTTTATTATTGGCATTTGGGGAGGGAAGCAAAGGATATATGCGACGTTTAAGTTATTTCTT
TATACATTAACTGGCTCATTATTATTTCTGCTTGGATTGGTGTATATCTATAGTACTTTTGGGACGTTTAATATACAAAA
ATTAGCTACATTAGTGCCAAGCCTTGATCTTGAAGTGCAGTCATTGCTGTGGATTACATTTTTTATTTCTTTTGCAATAA
AAGTACCTATGTTTCCATTTCACACTTGGCTTCCTGATGCGCATGTGCAATCACCAACTTCTGGATCTGTGATTTTAGCT
GGCTTGCTTATTAAAATGGGGGGATATGGATTTTTAAGGTTTTCTATTCCAATGCTTCCTCAGGCAAGTTTGTATTTTTC
AAATTTCGTTGTTGTGCTGAGCATTATTGCGGTGATATATGCTTCTCTAGTTGCGTTTGCTCAAGATGATATAAAGAAGT
TAATAGCTTATTCTTCAATAGCACATATGGGGATCGTTACTGCTGGCCTCTTTTCATTTTGTGAGGAAGGAGTACTGGGT
AGCATATTTCAAATGATTAGTCATGGCCTTATTTCTGCTGCTTTATTTTTATGTGTTGGAATGCTATATACTCGAACTGG
AACTTTGGAGATTGCAAAATATTTTGGCATAGTAAACACAATGCCAAAATTTGGTTTTATGTTCATTTTATTTTCAATGG
CTTCAATAGGGCTACCTGGAACATCTGGGTTCGCAGGTGAGTTTTTGGCTATGGTTGGGATGTTTAAGAGCATAGGGTTT
TTTACAGGATTTATCGCACTTGGCACTATTTTAAGCGCAGTTTATATGCTGAATTTATGTAAGCAAATAATATGGGGGGT
TAGCTATTCTAAATTATTAAATAACCGTTTGGATAGCATAGAATTTTCTGTCTTAATTCTGCTTGCAGTGTTTGTTATTT
TGCTTGGATTTTACCCAACCCTTGCACTGAATTATTTGAAGCCATGTATGGCAAATTTGTTAGTCAAATATAATGCGCTA
TGA

Upstream 100 bases:

>100_bases
TTCTATAAAGTTGCAGACTGGTTATATATTTGATTATGCATTTATTATGTTTGTTACTCTAATAATCGGTGCTTTATATA
TTATTGGAATTAAATAGAAG

Downstream 100 bases:

>100_bases
ATTATATACAGATATTGCCGGAAATGTTCTCTATTATCTCCTCGTTAGTGTTGCTACTGCTTGGGATTATATTTAACCGC
AGAACTATCAACTTATTAGC

Product: NADH dehydrogenase I, M subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLLLSIFLLPLIGALILSSIRINHQSIHLKFLALFFAILPFLLSIVACIEFDYNNADFQFVSYPIKNVGIGIDGISLLFL
LLTTFLFVICILYNCKMSYTTLKAYMALFLLLESFVVGFFVSLNAISFYVFFEAVLIPMFFIIGIWGGKQRIYATFKLFL
YTLTGSLLFLLGLVYIYSTFGTFNIQKLATLVPSLDLEVQSLLWITFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSIAHMGIVTAGLFSFCEEGVLG
SIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNTMPKFGFMFILFSMASIGLPGTSGFAGEFLAMVGMFKSIGF
FTGFIALGTILSAVYMLNLCKQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL

Sequences:

>Translated_480_residues
MLLLSIFLLPLIGALILSSIRINHQSIHLKFLALFFAILPFLLSIVACIEFDYNNADFQFVSYPIKNVGIGIDGISLLFL
LLTTFLFVICILYNCKMSYTTLKAYMALFLLLESFVVGFFVSLNAISFYVFFEAVLIPMFFIIGIWGGKQRIYATFKLFL
YTLTGSLLFLLGLVYIYSTFGTFNIQKLATLVPSLDLEVQSLLWITFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSIAHMGIVTAGLFSFCEEGVLG
SIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNTMPKFGFMFILFSMASIGLPGTSGFAGEFLAMVGMFKSIGF
FTGFIALGTILSAVYMLNLCKQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL
>Mature_480_residues
MLLLSIFLLPLIGALILSSIRINHQSIHLKFLALFFAILPFLLSIVACIEFDYNNADFQFVSYPIKNVGIGIDGISLLFL
LLTTFLFVICILYNCKMSYTTLKAYMALFLLLESFVVGFFVSLNAISFYVFFEAVLIPMFFIIGIWGGKQRIYATFKLFL
YTLTGSLLFLLGLVYIYSTFGTFNIQKLATLVPSLDLEVQSLLWITFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSIAHMGIVTAGLFSFCEEGVLG
SIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNTMPKFGFMFILFSMASIGLPGTSGFAGEFLAMVGMFKSIGF
FTGFIALGTILSAVYMLNLCKQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=335, Percent_Identity=33.134328358209, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI251831117, Length=266, Percent_Identity=25.9398496240602, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788613, Length=425, Percent_Identity=36.7058823529412, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1788827, Length=347, Percent_Identity=25.9365994236311, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788831, Length=304, Percent_Identity=27.3026315789474, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788829, Length=408, Percent_Identity=25.9803921568627, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI2367154, Length=243, Percent_Identity=26.3374485596708, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788614, Length=307, Percent_Identity=25.0814332247557, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693160, Length=374, Percent_Identity=24.331550802139, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53317; Mature: 53317

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLSIFLLPLIGALILSSIRINHQSIHLKFLALFFAILPFLLSIVACIEFDYNNADFQF
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
VSYPIKNVGIGIDGISLLFLLLTTFLFVICILYNCKMSYTTLKAYMALFLLLESFVVGFF
EECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VSLNAISFYVFFEAVLIPMFFIIGIWGGKQRIYATFKLFLYTLTGSLLFLLGLVYIYSTF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTFNIQKLATLVPSLDLEVQSLLWITFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHH
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSI
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHMGIVTAGLFSFCEEGVLGSIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MPKFGFMFILFSMASIGLPGTSGFAGEFLAMVGMFKSIGFFTGFIALGTILSAVYMLNLC
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLLSIFLLPLIGALILSSIRINHQSIHLKFLALFFAILPFLLSIVACIEFDYNNADFQF
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
VSYPIKNVGIGIDGISLLFLLLTTFLFVICILYNCKMSYTTLKAYMALFLLLESFVVGFF
EECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VSLNAISFYVFFEAVLIPMFFIIGIWGGKQRIYATFKLFLYTLTGSLLFLLGLVYIYSTF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTFNIQKLATLVPSLDLEVQSLLWITFFISFAIKVPMFPFHTWLPDAHVQSPTSGSVILA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHH
GLLIKMGGYGFLRFSIPMLPQASLYFSNFVVVLSIIAVIYASLVAFAQDDIKKLIAYSSI
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHMGIVTAGLFSFCEEGVLGSIFQMISHGLISAALFLCVGMLYTRTGTLEIAKYFGIVNT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MPKFGFMFILFSMASIGLPGTSGFAGEFLAMVGMFKSIGFFTGFIALGTILSAVYMLNLC
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQIIWGVSYSKLLNNRLDSIEFSVLILLAVFVILLGFYPTLALNYLKPCMANLLVKYNAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA