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The map label for this gene is ispDF [H]

Identifier: 190571363

GI number: 190571363

Start: 1037697

End: 1038902

Strand: Reverse

Name: ispDF [H]

Synonym: WPa_0969

Alternate gene names: 190571363

Gene position: 1038902-1037697 (Counterclockwise)

Preceding gene: 190571376

Following gene: 190571359

Centisome position: 70.08

GC content: 34.25

Gene sequence:

>1206_bases
ATGGTAAGCATGTACACTAACAGACTTGAGGAATACAAGATAGCAGCATTAATAGTTGCAGCAGGAGTAGGCAGTAGATG
CAATGCCAAAATCCCTAAACAGTATATAAAATTGGCAGGTAAGTCTGTTTTATTTCACACAATTAGGAAATTCTTAGCTA
ATCAGTACATAAGTTACATAAGAGTGGTAGTGAATGAAAGTCAAAAAGATTTCTATAAAGAAGTTACGTCATCAACTATA
GATACTAAATTGCTGAGTCCAGTATGTGGAGGAAAAAGCAGGCAGAGTTCAGTTAAACTTGGACTGGAGAGTTTGCAAAA
GATCAATCCAGATTTTGTTATTATACATGACGCTTGTAGGCCTTTTGTATCAAATATTCTAATAAACAACTTGATTGAAT
CTATGATTAATGGTCAATATACAGGAGTAGTTCCAGCAATAGAAGTCGAGGATACTATATCGTTGGTGAGTAATAATTTC
ATTGAATCTACGATTTCAAGAGAAAAACTTAGAGCCATGCAAACTCCTCAAATTTTTAATTTCAGAGAATTATTATCGTG
CCATCAATCAGTCAAAGAATTTACTGATGATTCATCACTAATGGTAGAGCATAAAAAACATGTTGCGATTATTGAAGGTG
AGAAAAAGAATTTTAAGTTAACTACAAAAGAGGATATCAATATGGCAAAACTTCTCTTTGGAGAGCCAAAATTCCGTGTC
GGAACCGGTTATGATATACATAAATTTGTTAAAATTCAAAATGATGCTAAGAGCTTTATAAAAATTTGCGGTGTGGAAAT
TGAGCATAATATGGCAATAGAGGCACATTCCGATGGTGATGTTGCAATACATGCAATCGTTGATGCAATACTCGGAGCAC
TAGGATGTGGCGATATAGGAGAACATTTTCCTCCTAATTCCTCTAAATGGAAGGATTGCAATTCATCTCATTTTCTTGAT
TTTGCTGCTAAAAAAGCACAGGAAAAAGGATATAGCGTGTCTAATTTAGATATTACTATAGTTTGTGAAGAGCCTAAAAT
ATCGCCTTACAAAGCAGGAATGAAGGAATTCATATCAAAAATATTAGAAATTAATGGTGAATTCCTAAGTATCAAAGCAA
CCACAGCGGAAAAATTAGGTTCTATTGGCAGAAACGAGGGAATAGCAACGTATGCTTCTGTGCTATTGCGTGCAAATTTT
TATTGA

Upstream 100 bases:

>100_bases
ATTTTCCTCTAGCATATATTACACCTAGTAAAAGAATATTCTTTTTTGAGAATTGGATCAACTTTTTTAAAATGCTAGAT
AACTTGTATTAACACCGAAA

Downstream 100 bases:

>100_bases
AGGTAGGAACGAAATAACTTACTTGACAAACTCCACCATTTCACTTCCATTGCAAGGAAAGTGGGAAGGTTTGTCAAGAA
ATTTCTATGGGCTACTTTCG

Product: ispD/ispF bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MVSMYTNRLEEYKIAALIVAAGVGSRCNAKIPKQYIKLAGKSVLFHTIRKFLANQYISYIRVVVNESQKDFYKEVTSSTI
DTKLLSPVCGGKSRQSSVKLGLESLQKINPDFVIIHDACRPFVSNILINNLIESMINGQYTGVVPAIEVEDTISLVSNNF
IESTISREKLRAMQTPQIFNFRELLSCHQSVKEFTDDSSLMVEHKKHVAIIEGEKKNFKLTTKEDINMAKLLFGEPKFRV
GTGYDIHKFVKIQNDAKSFIKICGVEIEHNMAIEAHSDGDVAIHAIVDAILGALGCGDIGEHFPPNSSKWKDCNSSHFLD
FAAKKAQEKGYSVSNLDITIVCEEPKISPYKAGMKEFISKILEINGEFLSIKATTAEKLGSIGRNEGIATYASVLLRANF
Y

Sequences:

>Translated_401_residues
MVSMYTNRLEEYKIAALIVAAGVGSRCNAKIPKQYIKLAGKSVLFHTIRKFLANQYISYIRVVVNESQKDFYKEVTSSTI
DTKLLSPVCGGKSRQSSVKLGLESLQKINPDFVIIHDACRPFVSNILINNLIESMINGQYTGVVPAIEVEDTISLVSNNF
IESTISREKLRAMQTPQIFNFRELLSCHQSVKEFTDDSSLMVEHKKHVAIIEGEKKNFKLTTKEDINMAKLLFGEPKFRV
GTGYDIHKFVKIQNDAKSFIKICGVEIEHNMAIEAHSDGDVAIHAIVDAILGALGCGDIGEHFPPNSSKWKDCNSSHFLD
FAAKKAQEKGYSVSNLDITIVCEEPKISPYKAGMKEFISKILEINGEFLSIKATTAEKLGSIGRNEGIATYASVLLRANF
Y
>Mature_401_residues
MVSMYTNRLEEYKIAALIVAAGVGSRCNAKIPKQYIKLAGKSVLFHTIRKFLANQYISYIRVVVNESQKDFYKEVTSSTI
DTKLLSPVCGGKSRQSSVKLGLESLQKINPDFVIIHDACRPFVSNILINNLIESMINGQYTGVVPAIEVEDTISLVSNNF
IESTISREKLRAMQTPQIFNFRELLSCHQSVKEFTDDSSLMVEHKKHVAIIEGEKKNFKLTTKEDINMAKLLFGEPKFRV
GTGYDIHKFVKIQNDAKSFIKICGVEIEHNMAIEAHSDGDVAIHAIVDAILGALGCGDIGEHFPPNSSKWKDCNSSHFLD
FAAKKAQEKGYSVSNLDITIVCEEPKISPYKAGMKEFISKILEINGEFLSIKATTAEKLGSIGRNEGIATYASVLLRANF
Y

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=236, Percent_Identity=25.4237288135593, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=40.251572327044, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789104, Length=234, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 44632; Mature: 44632

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSMYTNRLEEYKIAALIVAAGVGSRCNAKIPKQYIKLAGKSVLFHTIRKFLANQYISYI
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RVVVNESQKDFYKEVTSSTIDTKLLSPVCGGKSRQSSVKLGLESLQKINPDFVIIHDACR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHH
PFVSNILINNLIESMINGQYTGVVPAIEVEDTISLVSNNFIESTISREKLRAMQTPQIFN
HHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FRELLSCHQSVKEFTDDSSLMVEHKKHVAIIEGEKKNFKLTTKEDINMAKLLFGEPKFRV
HHHHHHHHHHHHHHCCCCHHEEECCCCEEEEECCCCCEEEECCCCCCHHHHHHCCCCEEE
GTGYDIHKFVKIQNDAKSFIKICGVEIEHNMAIEAHSDGDVAIHAIVDAILGALGCGDIG
CCCCCHHHHEEEHHHHHHHHHHHCCEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
EHFPPNSSKWKDCNSSHFLDFAAKKAQEKGYSVSNLDITIVCEEPKISPYKAGMKEFISK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
ILEINGEFLSIKATTAEKLGSIGRNEGIATYASVLLRANFY
HHHCCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSMYTNRLEEYKIAALIVAAGVGSRCNAKIPKQYIKLAGKSVLFHTIRKFLANQYISYI
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RVVVNESQKDFYKEVTSSTIDTKLLSPVCGGKSRQSSVKLGLESLQKINPDFVIIHDACR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHH
PFVSNILINNLIESMINGQYTGVVPAIEVEDTISLVSNNFIESTISREKLRAMQTPQIFN
HHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FRELLSCHQSVKEFTDDSSLMVEHKKHVAIIEGEKKNFKLTTKEDINMAKLLFGEPKFRV
HHHHHHHHHHHHHHCCCCHHEEECCCCEEEEECCCCCEEEECCCCCCHHHHHHCCCCEEE
GTGYDIHKFVKIQNDAKSFIKICGVEIEHNMAIEAHSDGDVAIHAIVDAILGALGCGDIG
CCCCCHHHHEEEHHHHHHHHHHHCCEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
EHFPPNSSKWKDCNSSHFLDFAAKKAQEKGYSVSNLDITIVCEEPKISPYKAGMKEFISK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
ILEINGEFLSIKATTAEKLGSIGRNEGIATYASVLLRANFY
HHHCCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA