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The map label for this gene is def

Identifier: 190571318

GI number: 190571318

Start: 992328

End: 992867

Strand: Reverse

Name: def

Synonym: WPa_0923

Alternate gene names: 190571318

Gene position: 992867-992328 (Counterclockwise)

Preceding gene: 190571321

Following gene: 190571317

Centisome position: 66.97

GC content: 36.11

Gene sequence:

>540_bases
ATGTCTAAATTGCCAATTGTAATTGCCCCTGATGAAAGGTTAACTACACGTGCCAGTGAAGTAATAGATATAACCGATAA
AATTAAAGAATTAGTAAACGACATGTTCGAAACTATGTACTATGCAGAAGGTCTTGGTCTTGCTGCAGTACAAGTTGGAG
TGCTGAAGAGAATTTTTATCATGGACGTTCAGCCAGAGAAGGCTGAAGATGGGCCAGCAGGATATGAATCAACTGGCAAA
TTTTGCATGATTAACCCTGAAATTACAGAATTATCGGGTGAACAAGTAATTCTCAAAGAAGGGTGCCTTTCAATTCCAGA
GCAAAGCCATGAAATTAAGCGTCCAAAATATTTAACTGTGAAATATAAAAATTTAAATAACGAAGAACAGACGCTAAAAG
CTAGTGGTTGGCTTGCAAGGTGTATTCAGCATGAGTTAGATCATTTAAATGGTATATTATACGTTAGACATTTGTCTAAA
TTGAAGTACGATATGGCTATGAAAAAAGCTCAAAAGGTTAAGAGGCACTATGAACAATGA

Upstream 100 bases:

>100_bases
CTTCCTTTAATAATTTATATTTCTATTAAAAATACAGCAACATTTTAATATACCCAAAATACTGTACAAAGGAAAAAATA
AAACATAAAATTTTAATTTT

Downstream 100 bases:

>100_bases
AGATCTAGAATTGTTGAAGTTCTATCATGAAGTGGGTGTTGACTGCACGCTAACTGAAGGTGAAGAGGAAAAAAAAGTGG
AAAATGAGAGAGCTGCACAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSKLPIVIAPDERLTTRASEVIDITDKIKELVNDMFETMYYAEGLGLAAVQVGVLKRIFIMDVQPEKAEDGPAGYESTGK
FCMINPEITELSGEQVILKEGCLSIPEQSHEIKRPKYLTVKYKNLNNEEQTLKASGWLARCIQHELDHLNGILYVRHLSK
LKYDMAMKKAQKVKRHYEQ

Sequences:

>Translated_179_residues
MSKLPIVIAPDERLTTRASEVIDITDKIKELVNDMFETMYYAEGLGLAAVQVGVLKRIFIMDVQPEKAEDGPAGYESTGK
FCMINPEITELSGEQVILKEGCLSIPEQSHEIKRPKYLTVKYKNLNNEEQTLKASGWLARCIQHELDHLNGILYVRHLSK
LKYDMAMKKAQKVKRHYEQ
>Mature_178_residues
SKLPIVIAPDERLTTRASEVIDITDKIKELVNDMFETMYYAEGLGLAAVQVGVLKRIFIMDVQPEKAEDGPAGYESTGKF
CMINPEITELSGEQVILKEGCLSIPEQSHEIKRPKYLTVKYKNLNNEEQTLKASGWLARCIQHELDHLNGILYVRHLSKL
KYDMAMKKAQKVKRHYEQ

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=175, Percent_Identity=41.7142857142857, Blast_Score=129, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645728, Length=154, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=29.1139240506329, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_WOLPP (B3CMB1)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975676.1
- EnsemblBacteria:   EBWOLT00000000034
- GeneID:   6384748
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0923
- GeneTree:   EBGT00050000031224
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- ProtClustDB:   PRK00150
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20469; Mature: 20338

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLPIVIAPDERLTTRASEVIDITDKIKELVNDMFETMYYAEGLGLAAVQVGVLKRIFI
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MDVQPEKAEDGPAGYESTGKFCMINPEITELSGEQVILKEGCLSIPEQSHEIKRPKYLTV
EECCCCCCCCCCCCCCCCCCEEEECCCHHHCCCCEEEEHHCCCCCCCCCHHCCCCEEEEE
KYKNLNNEEQTLKASGWLARCIQHELDHLNGILYVRHLSKLKYDMAMKKAQKVKRHYEQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLPIVIAPDERLTTRASEVIDITDKIKELVNDMFETMYYAEGLGLAAVQVGVLKRIFI
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MDVQPEKAEDGPAGYESTGKFCMINPEITELSGEQVILKEGCLSIPEQSHEIKRPKYLTV
EECCCCCCCCCCCCCCCCCCEEEECCCHHHCCCCEEEEHHCCCCCCCCCHHCCCCEEEEE
KYKNLNNEEQTLKASGWLARCIQHELDHLNGILYVRHLSKLKYDMAMKKAQKVKRHYEQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA