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The map label for this gene is proP [H]

Identifier: 190571321

GI number: 190571321

Start: 995174

End: 996385

Strand: Reverse

Name: proP [H]

Synonym: WPa_0926

Alternate gene names: 190571321

Gene position: 996385-995174 (Counterclockwise)

Preceding gene: 190571323

Following gene: 190571318

Centisome position: 67.21

GC content: 34.41

Gene sequence:

>1212_bases
GTGCAACAAATAACAAAAGTAATACTATCAAGTTTAATTTGTAACACTTTGTTGTGGTATGATCACATGCTTTTCAGCCA
TCTAATAAGCATAATCGGAGATGTGTTTTTTCCATCAGACGACAAATTAGTCAGCATACTTAAAGCTTTTGGAGTGTTTG
CAGTGGGTTTCTTAATAAGACCAATTGGTGCTGCTATATTTGGCCACATTGGCGATAAATATGGAAGAAGGATTGCTCTA
TTTCTCTCTATCATATTGATGACTCTATCAACCGTACTTATTGCATTTGTTCCAGGCTATCAGAACATTGGGGTACTCGC
ATCAGTACTGGTGGTATGTTTAAGATTATTGCAGGGTATATCTCTTGGAGGAGAGGCAGGAAATGCCCCATTTTTAATAG
AGCATGCTCCTAAAAATAAAAGAGGATTCTTTGGCAGTATTGAAGTATTGAGCGCAATTCTTGGCTCAATATTAAGTCTT
ATAGTGGTGCTTATATGCAAAAAAGTATCCGATTTTGAATCTTGGGGATGGAGATTGCCTTTTATTTTCAGTTTTGTAAT
AGGATTAATCAGTATATATATGAGATATATACTCGATGAAAGCCCAGAATATAAAAAGCAGGAAAAGCCACATGAATTAC
CGTTAAAAGAACTGTTAAATGGCTATAAAAAACCTTTTCTAATATCAATTGGAGTTGACATAGTTGAGAATGCATCTCTT
TACATATATTTAGTGTTTTTTAATCTACTTGCATCAACAATAAACACTCACTTTAATCACGCAGTAGAAATATTGAATCA
GGTTGCACTTGGAGGATTGACTATACTATTTGCAATACTTTCTGATAAGGTAGGGAGAGAAAGAGTCATGAAATTTGCAT
TTGTGACTTTTATAGTAGTAAGTTATCCAGTTTTTTCAATGATACTAAGTGGTAATAATTTGCTCACCATAATGGCGCAC
GTTCTTTTTATAATCCCAATAGCTGCTTCTCTTGGTCCTGTTAGCGCGTTTATGTGTGAATTATTCCCAATAAAGGTACG
ATACAGTGGATTTGGTTTATCAAGAAATATAGCTTCGGGATTTTTTGGTGGTCTTGCACCATTTATATGCACAACTATTA
TCAAAGTTACAGGACAAGGAACTTTAGCAAGTTTTTACATGATTTTCTGTGCATTAATTGGGTTAGTTGCTATATCACAA
GTTAAGAGTTAG

Upstream 100 bases:

>100_bases
AAATTTAATAAGATAATATTATTAATATAACATTTACATAATTCACGTTTGAAAAAGTAAGTAATATTACTATAATATCA
AACAAATTACGGAAGATTTC

Downstream 100 bases:

>100_bases
ATTTTTTCAAAAACCAAGTGTTTAAAAAATCTAATCATAAGGTGGAAAATGCCAATTTTTAGGTGATAGTGTAAATATTT
CAACTCCATCTTTTGTGACT

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MQQITKVILSSLICNTLLWYDHMLFSHLISIIGDVFFPSDDKLVSILKAFGVFAVGFLIRPIGAAIFGHIGDKYGRRIAL
FLSIILMTLSTVLIAFVPGYQNIGVLASVLVVCLRLLQGISLGGEAGNAPFLIEHAPKNKRGFFGSIEVLSAILGSILSL
IVVLICKKVSDFESWGWRLPFIFSFVIGLISIYMRYILDESPEYKKQEKPHELPLKELLNGYKKPFLISIGVDIVENASL
YIYLVFFNLLASTINTHFNHAVEILNQVALGGLTILFAILSDKVGRERVMKFAFVTFIVVSYPVFSMILSGNNLLTIMAH
VLFIIPIAASLGPVSAFMCELFPIKVRYSGFGLSRNIASGFFGGLAPFICTTIIKVTGQGTLASFYMIFCALIGLVAISQ
VKS

Sequences:

>Translated_403_residues
MQQITKVILSSLICNTLLWYDHMLFSHLISIIGDVFFPSDDKLVSILKAFGVFAVGFLIRPIGAAIFGHIGDKYGRRIAL
FLSIILMTLSTVLIAFVPGYQNIGVLASVLVVCLRLLQGISLGGEAGNAPFLIEHAPKNKRGFFGSIEVLSAILGSILSL
IVVLICKKVSDFESWGWRLPFIFSFVIGLISIYMRYILDESPEYKKQEKPHELPLKELLNGYKKPFLISIGVDIVENASL
YIYLVFFNLLASTINTHFNHAVEILNQVALGGLTILFAILSDKVGRERVMKFAFVTFIVVSYPVFSMILSGNNLLTIMAH
VLFIIPIAASLGPVSAFMCELFPIKVRYSGFGLSRNIASGFFGGLAPFICTTIIKVTGQGTLASFYMIFCALIGLVAISQ
VKS
>Mature_403_residues
MQQITKVILSSLICNTLLWYDHMLFSHLISIIGDVFFPSDDKLVSILKAFGVFAVGFLIRPIGAAIFGHIGDKYGRRIAL
FLSIILMTLSTVLIAFVPGYQNIGVLASVLVVCLRLLQGISLGGEAGNAPFLIEHAPKNKRGFFGSIEVLSAILGSILSL
IVVLICKKVSDFESWGWRLPFIFSFVIGLISIYMRYILDESPEYKKQEKPHELPLKELLNGYKKPFLISIGVDIVENASL
YIYLVFFNLLASTINTHFNHAVEILNQVALGGLTILFAILSDKVGRERVMKFAFVTFIVVSYPVFSMILSGNNLLTIMAH
VLFIIPIAASLGPVSAFMCELFPIKVRYSGFGLSRNIASGFFGGLAPFICTTIIKVTGQGTLASFYMIFCALIGLVAISQ
VKS

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=414, Percent_Identity=30.1932367149758, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1788292, Length=390, Percent_Identity=33.3333333333333, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1788942, Length=387, Percent_Identity=29.9741602067183, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789941, Length=412, Percent_Identity=32.2815533980583, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=25.1908396946565, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082231, Length=183, Percent_Identity=25.6830601092896, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44315; Mature: 44315

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQITKVILSSLICNTLLWYDHMLFSHLISIIGDVFFPSDDKLVSILKAFGVFAVGFLIR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
PIGAAIFGHIGDKYGRRIALFLSIILMTLSTVLIAFVPGYQNIGVLASVLVVCLRLLQGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
SLGGEAGNAPFLIEHAPKNKRGFFGSIEVLSAILGSILSLIVVLICKKVSDFESWGWRLP
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FIFSFVIGLISIYMRYILDESPEYKKQEKPHELPLKELLNGYKKPFLISIGVDIVENASL
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEECCHHHHCCHH
YIYLVFFNLLASTINTHFNHAVEILNQVALGGLTILFAILSDKVGRERVMKFAFVTFIVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYPVFSMILSGNNLLTIMAHVLFIIPIAASLGPVSAFMCELFPIKVRYSGFGLSRNIASG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCHHHHHHH
FFGGLAPFICTTIIKVTGQGTLASFYMIFCALIGLVAISQVKS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQITKVILSSLICNTLLWYDHMLFSHLISIIGDVFFPSDDKLVSILKAFGVFAVGFLIR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
PIGAAIFGHIGDKYGRRIALFLSIILMTLSTVLIAFVPGYQNIGVLASVLVVCLRLLQGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
SLGGEAGNAPFLIEHAPKNKRGFFGSIEVLSAILGSILSLIVVLICKKVSDFESWGWRLP
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FIFSFVIGLISIYMRYILDESPEYKKQEKPHELPLKELLNGYKKPFLISIGVDIVENASL
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEECCHHHHCCHH
YIYLVFFNLLASTINTHFNHAVEILNQVALGGLTILFAILSDKVGRERVMKFAFVTFIVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYPVFSMILSGNNLLTIMAHVLFIIPIAASLGPVSAFMCELFPIKVRYSGFGLSRNIASG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCHHHHHHH
FFGGLAPFICTTIIKVTGQGTLASFYMIFCALIGLVAISQVKS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]