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The map label for this gene is aceF [H]

Identifier: 190571193

GI number: 190571193

Start: 870074

End: 871336

Strand: Reverse

Name: aceF [H]

Synonym: WPa_0791

Alternate gene names: 190571193

Gene position: 871336-870074 (Counterclockwise)

Preceding gene: 190571195

Following gene: 190571192

Centisome position: 58.78

GC content: 35.47

Gene sequence:

>1263_bases
ATGCCTATAGAAATATTGATGCCTGCTCTTTCGCCAACAATGAGTAAAACTGGAGGAAAAATTGTAAAGTGGTGTAAAAA
AGAACAAGATAAAGTTGAAATAGGTGATGTAATCGCTGAAATTGAGACTGATAAAGCCATAATGGAGTTTGAATCTGTCG
ATGAAGGAGTTTTGGCAAAAATTTTAGTGTCGGAAGGAACAAGTGGCGTGCCTGTAAATCAACTGATAGCTCTAATGTTA
GAGGAAGGGGAAGATAAAAGCGCACTTGATTTAGCTTCTGCCATCAATACCAAGGTTGAGAAGGAGGTTGAAGCCGATTT
TTCAGTATCATCCAACCCTTCGATTTCATCTAGCTCTTTAATGTCATCCCAGTGCGTGACACTGGGATCTAAAAAAGAGG
ATAGAGCAACAGAAAATAGAATAAAAGTAAGCCCCTTAGCTAAAAAAATAGCTCAAAATGAAGGTGTTGATATAAAGCGA
TTAAAAGGTACAGGTCCATATGGTCGTATCATCAAAGCTGATGTATTAGAATTTTTAGATCAAACTAAAAGCTATGAGAG
ATTTGAGGAAAATACAACAGTTGAAGTAAGTAATATGCGCCAAGTGATAGCGCAGCGCCTAGTTGAATCTAAGCAAAATA
TTCCACACTTTTATTTAACTGTAGACTGCCACGTTGATAAGCTAATATCGCTCAAAAATGAGGTTAATTCAGCAAATGAG
AACAATAAAGTAACAATTAATGACTTAATTATAAAAGCTGTGGCTTTCAGCATGAAAAAATTTCCTGATATAAACTCATC
GTGGATAGATACTAAAATAGTAAGATACTCAAATATAGATATCTCAATTGCTGTAGCGCTTGAGGATGGACTGATTACTC
CTATAGTAAAAAATGCTGATGAAAAAAGTGTTTTATCTATATCAAAAGAAGTGAAAGATTTAGTAAATAGAGCAAGATCT
GGAAAATTAAGGCCTGAAGAATTTCAAGGAGGAGGGTTTACCATTTCTAATTTAGGCATGTTTGGTATAAAAACTTTCAG
TGCTATAATCAATCCACCACAGTCTTGCATTATGGCTGTCGGCGCATCTAAAAAACAACCAGTTGTCATAAGTGAAAAAA
TAGAGATAGCAGAGGTAATGACAGTTACTCTCTCTGTTGACCACAGAGCAGTTGATGGAGCACTCGGAGCAAAATTTTTA
AATGCCTTTAAGTACTATATAGAAAACCCTACGGTGATGCTTCTTGAGCCTTTATTATTATAA

Upstream 100 bases:

>100_bases
CATCACGTACTAGGATGATATAAGTATCATAATTTACGATTAGATTGACATAGGGTAAAGAGTAAATTAAAAGTTAAATA
GATCTTTTAATACAGAATAA

Downstream 100 bases:

>100_bases
TAAAATTAATACCTCTTAGTTTATACTTTTATTAGTTAATAGGTTTAGAGGTAAAATATGACTTATAAAAAGATAGAAAA
ACGTAGTATATCTTTTGATG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPIEILMPALSPTMSKTGGKIVKWCKKEQDKVEIGDVIAEIETDKAIMEFESVDEGVLAKILVSEGTSGVPVNQLIALML
EEGEDKSALDLASAINTKVEKEVEADFSVSSNPSISSSSLMSSQCVTLGSKKEDRATENRIKVSPLAKKIAQNEGVDIKR
LKGTGPYGRIIKADVLEFLDQTKSYERFEENTTVEVSNMRQVIAQRLVESKQNIPHFYLTVDCHVDKLISLKNEVNSANE
NNKVTINDLIIKAVAFSMKKFPDINSSWIDTKIVRYSNIDISIAVALEDGLITPIVKNADEKSVLSISKEVKDLVNRARS
GKLRPEEFQGGGFTISNLGMFGIKTFSAIINPPQSCIMAVGASKKQPVVISEKIEIAEVMTVTLSVDHRAVDGALGAKFL
NAFKYYIENPTVMLLEPLLL

Sequences:

>Translated_420_residues
MPIEILMPALSPTMSKTGGKIVKWCKKEQDKVEIGDVIAEIETDKAIMEFESVDEGVLAKILVSEGTSGVPVNQLIALML
EEGEDKSALDLASAINTKVEKEVEADFSVSSNPSISSSSLMSSQCVTLGSKKEDRATENRIKVSPLAKKIAQNEGVDIKR
LKGTGPYGRIIKADVLEFLDQTKSYERFEENTTVEVSNMRQVIAQRLVESKQNIPHFYLTVDCHVDKLISLKNEVNSANE
NNKVTINDLIIKAVAFSMKKFPDINSSWIDTKIVRYSNIDISIAVALEDGLITPIVKNADEKSVLSISKEVKDLVNRARS
GKLRPEEFQGGGFTISNLGMFGIKTFSAIINPPQSCIMAVGASKKQPVVISEKIEIAEVMTVTLSVDHRAVDGALGAKFL
NAFKYYIENPTVMLLEPLLL
>Mature_419_residues
PIEILMPALSPTMSKTGGKIVKWCKKEQDKVEIGDVIAEIETDKAIMEFESVDEGVLAKILVSEGTSGVPVNQLIALMLE
EGEDKSALDLASAINTKVEKEVEADFSVSSNPSISSSSLMSSQCVTLGSKKEDRATENRIKVSPLAKKIAQNEGVDIKRL
KGTGPYGRIIKADVLEFLDQTKSYERFEENTTVEVSNMRQVIAQRLVESKQNIPHFYLTVDCHVDKLISLKNEVNSANEN
NKVTINDLIIKAVAFSMKKFPDINSSWIDTKIVRYSNIDISIAVALEDGLITPIVKNADEKSVLSISKEVKDLVNRARSG
KLRPEEFQGGGFTISNLGMFGIKTFSAIINPPQSCIMAVGASKKQPVVISEKIEIAEVMTVTLSVDHRAVDGALGAKFLN
AFKYYIENPTVMLLEPLLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=436, Percent_Identity=43.348623853211, Blast_Score=337, Evalue=9e-93,
Organism=Homo sapiens, GI203098816, Length=462, Percent_Identity=39.1774891774892, Blast_Score=298, Evalue=9e-81,
Organism=Homo sapiens, GI203098753, Length=462, Percent_Identity=39.1774891774892, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=48.7341772151899, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=35.0877192982456, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=26.1467889908257, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786946, Length=433, Percent_Identity=28.6374133949192, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786305, Length=301, Percent_Identity=27.2425249169435, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=42.0323325635104, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=35.5555555555556, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17537937, Length=444, Percent_Identity=26.3513513513513, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=397, Percent_Identity=26.7002518891688, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=38.0129589632829, Blast_Score=292, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6320352, Length=228, Percent_Identity=35.9649122807018, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321632, Length=216, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=45.8620689655172, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=45.7044673539519, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI18859875, Length=311, Percent_Identity=32.1543408360129, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=238, Percent_Identity=30.2521008403361, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46084; Mature: 45953

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMSKTGGKIVKWCKKEQDKVEIGDVIAEIETDKAIMEFESVDEGVLAK
CCCEEEEHHCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHH
ILVSEGTSGVPVNQLIALMLEEGEDKSALDLASAINTKVEKEVEADFSVSSNPSISSSSL
HHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
MSSQCVTLGSKKEDRATENRIKVSPLAKKIAQNEGVDIKRLKGTGPYGRIIKADVLEFLD
HHHHHHHCCCCCCCHHHHCCEEECHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
QTKSYERFEENTTVEVSNMRQVIAQRLVESKQNIPHFYLTVDCHVDKLISLKNEVNSANE
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCC
NNKVTINDLIIKAVAFSMKKFPDINSSWIDTKIVRYSNIDISIAVALEDGLITPIVKNAD
CCCEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCHHHCCCC
EKSVLSISKEVKDLVNRARSGKLRPEEFQGGGFTISNLGMFGIKTFSAIINPPQSCIMAV
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHH
GASKKQPVVISEKIEIAEVMTVTLSVDHRAVDGALGAKFLNAFKYYIENPTVMLLEPLLL
CCCCCCCEEEECCHHEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure 
PIEILMPALSPTMSKTGGKIVKWCKKEQDKVEIGDVIAEIETDKAIMEFESVDEGVLAK
CCEEEEHHCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHH
ILVSEGTSGVPVNQLIALMLEEGEDKSALDLASAINTKVEKEVEADFSVSSNPSISSSSL
HHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
MSSQCVTLGSKKEDRATENRIKVSPLAKKIAQNEGVDIKRLKGTGPYGRIIKADVLEFLD
HHHHHHHCCCCCCCHHHHCCEEECHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
QTKSYERFEENTTVEVSNMRQVIAQRLVESKQNIPHFYLTVDCHVDKLISLKNEVNSANE
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCC
NNKVTINDLIIKAVAFSMKKFPDINSSWIDTKIVRYSNIDISIAVALEDGLITPIVKNAD
CCCEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCHHHCCCC
EKSVLSISKEVKDLVNRARSGKLRPEEFQGGGFTISNLGMFGIKTFSAIINPPQSCIMAV
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHH
GASKKQPVVISEKIEIAEVMTVTLSVDHRAVDGALGAKFLNAFKYYIENPTVMLLEPLLL
CCCCCCCEEEECCHHEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA