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The map label for this gene is gltX

Identifier: 190571023

GI number: 190571023

Start: 667260

End: 668612

Strand: Reverse

Name: gltX

Synonym: WPa_0615

Alternate gene names: 190571023

Gene position: 668612-667260 (Counterclockwise)

Preceding gene: 190571026

Following gene: 190571021

Centisome position: 45.1

GC content: 37.1

Gene sequence:

>1353_bases
ATGCCAGGTATAGTCACTAGATTCGCTCCATCACCAACAGGATTTTTACATATTGGAGGAGCTCGCACAGCTTTGTTTAA
TTGGCTCTATGCGCAGCGTCATGGTGGCCAGTTTTTACTAAGGATTGAGGATACCGACAGAAAACGTTCAACACAGGAAG
CAATTGATGCAATAATAGATGGGTTAAAATGGCTTGGAATAAGCTATGATGGAGAAATAGTATATCAATCAAAAAGAATA
GATAGACATATTGAAGTTGCAAATCTTTTAGTTGAAAAGGGTAGGGCGTATCATTGTTACTGCCCCGAGAATGAAGTTGC
GGAGAAGAAAATAAGAGCAAGGGAAGAAGGAAAAATATATAAGCATAAGTGTACTCATTCAACATCAAATGCAGAGCCTG
TTGTTCGCTTCAAAGTGCCAGACTCAGAAGATATTGTTGTTGACGATAAAATATACGGCCAAGTTACAATAAGTAGCGAC
CAGCTTGATGACATAGTAATCTTGCGCTCTGACAATACTCCAACATATATTTTTGCTGTAGTGGTTGATGACCATGATGC
TGGAATAACCGATATTATACGTGGCTCTGATCATCTCACTAATACCTTCAAGCAATTACTAATATACCAAGCTCTTGATT
TTGATGTCCCACGCTTTGCACACGTACCGCTTATTCATGGAGAAGACGGGAATAAGTTATCTAAAAGGCATGGTGCAACA
AGTGTTTGCGATTATGAAAAGATGGGAATATTGCCACAGGCAATGCGTAATTACTTGCTAAGACTTGGCTGGAGTCATGG
CAACGATGAGATTATTAGCGATGAGCAAGCAATAGAGTGGTTTAATTTAGAAAGCATCGGTCGCTCACCTGCACGGCTCG
ATTTCAAAAAACTGGAGCATTTGAATAACCACTATATTAGTAATATGAGCAATGAAGATATTCTGACTCTAATGCTTAGA
GAAAATACTCTAACTGACAAAAAAAAGGGCTATTTACTGCAGGGACTGACAGAGCTGAAAAAAAGAGCAAACTATTTGAC
CGAATTATTGGACTTAGCAAAATTTTATATTCAAGTTGATTTAAGTGAAGAAGCTCAGCAAATTGTCAAATCTAACCTCA
ATGTAATTAAGTTACTTGCATCATTTCTTTCGAGTGTAGGTAGTGAAGATTGGAATAAGAATTTTTTATCTTCTCAGATT
AAGGAATTTTCAAAATTACATAATATAAAAATGAGCGATATATACCACTCATTACGCGCTCCAATAACTGGAATAATGGA
TGCGCCTGGAATCATCGATATCATGGTAATTCTTGGTAAAGATGAGTGTATAAAAAGATTGCAAGCAATTTAA

Upstream 100 bases:

>100_bases
AAAAATAAATGTAAATTGATCTTTCAAATTTACTATAGTTCCACGTTGTTGCAAGTACAAATTTTAGTTATCATCGGTTA
CGTTATCACAATAGACAAAT

Downstream 100 bases:

>100_bases
ATAAAAAATTGCTTGACAGACTCTAGTAGCTTCATTATAGTGGAGAATGAAGATTTTTTAGGATCCAAAATCTATCTTCA
CCTGCAGGTTTTTTCACCAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPGIVTRFAPSPTGFLHIGGARTALFNWLYAQRHGGQFLLRIEDTDRKRSTQEAIDAIIDGLKWLGISYDGEIVYQSKRI
DRHIEVANLLVEKGRAYHCYCPENEVAEKKIRAREEGKIYKHKCTHSTSNAEPVVRFKVPDSEDIVVDDKIYGQVTISSD
QLDDIVILRSDNTPTYIFAVVVDDHDAGITDIIRGSDHLTNTFKQLLIYQALDFDVPRFAHVPLIHGEDGNKLSKRHGAT
SVCDYEKMGILPQAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKKLEHLNNHYISNMSNEDILTLMLR
ENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQVDLSEEAQQIVKSNLNVIKLLASFLSSVGSEDWNKNFLSSQI
KEFSKLHNIKMSDIYHSLRAPITGIMDAPGIIDIMVILGKDECIKRLQAI

Sequences:

>Translated_450_residues
MPGIVTRFAPSPTGFLHIGGARTALFNWLYAQRHGGQFLLRIEDTDRKRSTQEAIDAIIDGLKWLGISYDGEIVYQSKRI
DRHIEVANLLVEKGRAYHCYCPENEVAEKKIRAREEGKIYKHKCTHSTSNAEPVVRFKVPDSEDIVVDDKIYGQVTISSD
QLDDIVILRSDNTPTYIFAVVVDDHDAGITDIIRGSDHLTNTFKQLLIYQALDFDVPRFAHVPLIHGEDGNKLSKRHGAT
SVCDYEKMGILPQAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKKLEHLNNHYISNMSNEDILTLMLR
ENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQVDLSEEAQQIVKSNLNVIKLLASFLSSVGSEDWNKNFLSSQI
KEFSKLHNIKMSDIYHSLRAPITGIMDAPGIIDIMVILGKDECIKRLQAI
>Mature_449_residues
PGIVTRFAPSPTGFLHIGGARTALFNWLYAQRHGGQFLLRIEDTDRKRSTQEAIDAIIDGLKWLGISYDGEIVYQSKRID
RHIEVANLLVEKGRAYHCYCPENEVAEKKIRAREEGKIYKHKCTHSTSNAEPVVRFKVPDSEDIVVDDKIYGQVTISSDQ
LDDIVILRSDNTPTYIFAVVVDDHDAGITDIIRGSDHLTNTFKQLLIYQALDFDVPRFAHVPLIHGEDGNKLSKRHGATS
VCDYEKMGILPQAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKKLEHLNNHYISNMSNEDILTLMLRE
NTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQVDLSEEAQQIVKSNLNVIKLLASFLSSVGSEDWNKNFLSSQIK
EFSKLHNIKMSDIYHSLRAPITGIMDAPGIIDIMVILGKDECIKRLQAI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=29.8568507157464, Blast_Score=216, Evalue=5e-56,
Organism=Escherichia coli, GI1788743, Length=458, Percent_Identity=40.6113537117904, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=34.1085271317829, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786895, Length=92, Percent_Identity=38.0434782608696, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=479, Percent_Identity=31.3152400835073, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=327, Percent_Identity=37.3088685015291, Blast_Score=215, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6324742, Length=132, Percent_Identity=32.5757575757576, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=235, Percent_Identity=28.0851063829787, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24665321, Length=483, Percent_Identity=33.3333333333333, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17864488, Length=95, Percent_Identity=37.8947368421053, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_WOLPP (B3CLF5)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975381.1
- EnsemblBacteria:   EBWOLT00000001233
- GeneID:   6385357
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0615
- GeneTree:   EBGT00050000030976
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51202; Mature: 51071

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIVTRFAPSPTGFLHIGGARTALFNWLYAQRHGGQFLLRIEDTDRKRSTQEAIDAIID
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHC
GLKWLGISYDGEIVYQSKRIDRHIEVANLLVEKGRAYHCYCPENEVAEKKIRAREEGKIY
CHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEE
KHKCTHSTSNAEPVVRFKVPDSEDIVVDDKIYGQVTISSDQLDDIVILRSDNTPTYIFAV
EEECCCCCCCCCCEEEEECCCCCCEEECCCEEEEEEECCCCCCCEEEEECCCCCEEEEEE
VVDDHDAGITDIIRGSDHLTNTFKQLLIYQALDFDVPRFAHVPLIHGEDGNKLSKRHGAT
EEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHCCCC
SVCDYEKMGILPQAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKKLEH
CCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECHHHHCCCCCCCCHHHHHH
LNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQVD
HHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LSEEAQQIVKSNLNVIKLLASFLSSVGSEDWNKNFLSSQIKEFSKLHNIKMSDIYHSLRA
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHC
PITGIMDAPGIIDIMVILGKDECIKRLQAI
CHHHCCCCCCCEEEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
PGIVTRFAPSPTGFLHIGGARTALFNWLYAQRHGGQFLLRIEDTDRKRSTQEAIDAIID
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHC
GLKWLGISYDGEIVYQSKRIDRHIEVANLLVEKGRAYHCYCPENEVAEKKIRAREEGKIY
CHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEE
KHKCTHSTSNAEPVVRFKVPDSEDIVVDDKIYGQVTISSDQLDDIVILRSDNTPTYIFAV
EEECCCCCCCCCCEEEEECCCCCCEEECCCEEEEEEECCCCCCCEEEEECCCCCEEEEEE
VVDDHDAGITDIIRGSDHLTNTFKQLLIYQALDFDVPRFAHVPLIHGEDGNKLSKRHGAT
EEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHCCCC
SVCDYEKMGILPQAMRNYLLRLGWSHGNDEIISDEQAIEWFNLESIGRSPARLDFKKLEH
CCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECHHHHCCCCCCCCHHHHHH
LNNHYISNMSNEDILTLMLRENTLTDKKKGYLLQGLTELKKRANYLTELLDLAKFYIQVD
HHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LSEEAQQIVKSNLNVIKLLASFLSSVGSEDWNKNFLSSQIKEFSKLHNIKMSDIYHSLRA
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHC
PITGIMDAPGIIDIMVILGKDECIKRLQAI
CHHHCCCCCCCEEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA