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The map label for this gene is pdxJ

Identifier: 190571021

GI number: 190571021

Start: 664878

End: 665585

Strand: Reverse

Name: pdxJ

Synonym: WPa_0613

Alternate gene names: 190571021

Gene position: 665585-664878 (Counterclockwise)

Preceding gene: 190571023

Following gene: 190571017

Centisome position: 44.9

GC content: 35.17

Gene sequence:

>708_bases
GTGAAATTGGGCGTTAATATTGATCACGTTGCAACCCTTCGCAACGCACGTGGAGCTTCTTATCCAGATCCATTAAAAGC
AGCAAAAATAGCTATTAATGCTGGAGCAGATTTTCTCACCGTACACTTACGAGAAGACAGAAGGCATATCAGAGATGAGG
ATGTATTCAATCTAAAACAAAACATTAGCACTGAGTTAAATCTTGAAATCGCAGCTACGGAAGAAATGCTCAAAATAGCA
AAAGAAGTAAAACCCTACTCGATTTGTATAGTACCAGAAAAAAGAGAAGAATTAACAACCGAAGGTGGCCTGAATATCGT
GAATATGCACAGTAAACTTTCTGGTATAATAGAGGAAATGCATAGCTTTGACATAAAAGTCTCACTGTTTATCGATCCAA
ATATTAATCAACTAAAATATCTTGAGAAGCTAGAAAGAAAGCCTGACATAATAGAAATTCACACGGGGGATTACTGTGAT
AATCCATCAGAGGAAAAATTAAAACTTATTACTAACTCTGCAGAATACATTAATAATCTAGGAATAGAATGCCACGCAGG
ACATGGCATAAATTATAAACACGCTAAAGAAATAAAAAAGATACCTCACATCTCAGCTCTTAATATAGGCCATTCTTTAA
TCAGCGAGGCTATATTTCATGGCTTACATAGTGTAACTAAAAAGATGAAAATGACAATATCTAAGTAG

Upstream 100 bases:

>100_bases
AGTATAACTGCTAGCTCCGTCTTTCAGCTATGGCATTAAACAATGACATGCATAAGATCTAATAATATGTATAATTTATA
AATAATTTTTGAGGTAAATT

Downstream 100 bases:

>100_bases
ATAATACTTTTTGCTTGCTATTTAAAATACTTAAAAGAGTTTGGCTTATTTTAAATTTTACCCTTGTCTTTTCAGGAACG
GTCATAATCTCACCATTTTG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKLGVNIDHVATLRNARGASYPDPLKAAKIAINAGADFLTVHLREDRRHIRDEDVFNLKQNISTELNLEIAATEEMLKIA
KEVKPYSICIVPEKREELTTEGGLNIVNMHSKLSGIIEEMHSFDIKVSLFIDPNINQLKYLEKLERKPDIIEIHTGDYCD
NPSEEKLKLITNSAEYINNLGIECHAGHGINYKHAKEIKKIPHISALNIGHSLISEAIFHGLHSVTKKMKMTISK

Sequences:

>Translated_235_residues
MKLGVNIDHVATLRNARGASYPDPLKAAKIAINAGADFLTVHLREDRRHIRDEDVFNLKQNISTELNLEIAATEEMLKIA
KEVKPYSICIVPEKREELTTEGGLNIVNMHSKLSGIIEEMHSFDIKVSLFIDPNINQLKYLEKLERKPDIIEIHTGDYCD
NPSEEKLKLITNSAEYINNLGIECHAGHGINYKHAKEIKKIPHISALNIGHSLISEAIFHGLHSVTKKMKMTISK
>Mature_235_residues
MKLGVNIDHVATLRNARGASYPDPLKAAKIAINAGADFLTVHLREDRRHIRDEDVFNLKQNISTELNLEIAATEEMLKIA
KEVKPYSICIVPEKREELTTEGGLNIVNMHSKLSGIIEEMHSFDIKVSLFIDPNINQLKYLEKLERKPDIIEIHTGDYCD
NPSEEKLKLITNSAEYINNLGIECHAGHGINYKHAKEIKKIPHISALNIGHSLISEAIFHGLHSVTKKMKMTISK

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=232, Percent_Identity=49.5689655172414, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_WOLPP (B3CLF3)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975379.1
- EnsemblBacteria:   EBWOLT00000000111
- GeneID:   6385356
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0613
- GeneTree:   EBGT00050000031522
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26491; Mature: 26491

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: ACT_SITE 42-42 ACT_SITE 69-69 ACT_SITE 188-188 BINDING 6-6 BINDING 17-17 BINDING 44-44 BINDING 49-49 BINDING 99-99 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGVNIDHVATLRNARGASYPDPLKAAKIAINAGADFLTVHLREDRRHIRDEDVFNLKQ
CCCCCCHHHHHHHHHCCCCCCCCCHHHHEEEEECCCCEEEEEEEHHHHHCCCCHHHHHHH
NISTELNLEIAATEEMLKIAKEVKPYSICIVPEKREELTTEGGLNIVNMHSKLSGIIEEM
CCCCEEEEEEECHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCEEEEHHHHHHHHHHHH
HSFDIKVSLFIDPNINQLKYLEKLERKPDIIEIHTGDYCDNPSEEKLKLITNSAEYINNL
HCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHCC
GIECHAGHGINYKHAKEIKKIPHISALNIGHSLISEAIFHGLHSVTKKMKMTISK
CCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLGVNIDHVATLRNARGASYPDPLKAAKIAINAGADFLTVHLREDRRHIRDEDVFNLKQ
CCCCCCHHHHHHHHHCCCCCCCCCHHHHEEEEECCCCEEEEEEEHHHHHCCCCHHHHHHH
NISTELNLEIAATEEMLKIAKEVKPYSICIVPEKREELTTEGGLNIVNMHSKLSGIIEEM
CCCCEEEEEEECHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCEEEEHHHHHHHHHHHH
HSFDIKVSLFIDPNINQLKYLEKLERKPDIIEIHTGDYCDNPSEEKLKLITNSAEYINNL
HCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHCC
GIECHAGHGINYKHAKEIKKIPHISALNIGHSLISEAIFHGLHSVTKKMKMTISK
CCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA