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The map label for this gene is tgt [H]

Identifier: 190570999

GI number: 190570999

Start: 636038

End: 637249

Strand: Direct

Name: tgt [H]

Synonym: WPa_0589

Alternate gene names: 190570999

Gene position: 636038-637249 (Clockwise)

Preceding gene: 190570998

Following gene: 190571002

Centisome position: 42.9

GC content: 37.38

Gene sequence:

>1212_bases
ATGGAAAAGTTCTCGTTTAGGATAACTAAGCAATCAGGTTCTGCAAGAGTTGGAACGATCAAAACTCCAAATGGAAGTAT
AGAAACACCAGCATTTATATTTTGTGCAACCAAAGCTGCTATTAAAGCTGCAGATATCGAGAGAATCAGAGAAGCAGGTA
CTCAGATAATACTTTCCAACACCTATCACCTAATGCTCCAACCGGGAGAGGATACTGTGGCAAAGCTTGGTGGCTTGCAC
AAGATGATGGGATGGAGTGGGCCAATGCTCACTGATTCTGGTGGATACCAGATATTTAGTTTAGGGCACGGGTCAGTTTC
GGAAGAAATAAAGGGAATAAGACAGAAGCAAAAAACTCTGATCAAAATTAACGAGGATGGGGCAATTTTTCGTTCTTACA
TAAATGGTAAAACTTATTGTCTAACTCCTGAAAAGTCGATACAAATTCAACAAAAATTAGGTGCAGATTTAATCCTAGTT
TTAGATGAATGTACTCCATTCCATGTCAGTAAAGAATACACAAGAAAATCAATGCTCATGAGCCATAGATGGGCTGAACG
TTCTTTAAATAAGTTTGAAAAAAATAATGATGGCAAACAGGCGCTTTATGGAATTAGTCAAGGCGGAGTGTATCAAGATT
TGCGTAGAGAAAGTTGTGATTTTATCAACGATTTGCCATTTTTTGGTCAAGCAATAGGTGGATCACTTGGTCAAAGTAAA
GAGCAGATGTATGATGTAGTTTCCTTTACTATGGAACATTTAAAAAAAGATAGGCCTACTCATTTGCTTGGTATTGGTGG
AATTGTAGACATTTTTCGTGGAGTAGAGCTTGGAGTTGATACATTTGATTGCGTGCATCCAACTCGCCTCGCAAGACATG
GTGGCGCGCTTGTTAAAGTAAAAAACAGAGATTCTATTTCTTCAAAGCGTAAAGAGCATATTAATTTGCGAAATCAACGA
TTCGAACTAGATGATAACCCAATTGAAAATGACTGTTTGTGCTTTACTTGCAGAAAACATTCAAGAGCCTATATACACCA
TCTACTGAAAGCTAAAGAGTTGCTGGCTTATACACTCGTTACCATTCACAATGTTTTCTTCATGAATAAGTTGATGGAAT
CAATAAGACAAGCAATATTGAATGATAGGCTAGATCAGGAAAAAAATAATTGGATTAGTGAAATACCGCTGCATTGTGAA
CTATATACCTAG

Upstream 100 bases:

>100_bases
ATGCGTTAATGCCCCTGTGGTGCAGATCAATAATGACTTTTATGAAAACCTCACTCCTGAAAAAGTGGAAAATATCATAG
CAGAACTTTCAAGTAAATAG

Downstream 100 bases:

>100_bases
TTCATATTTTGTACATGTGCGTTGCATTTTTTAATCTTCTTTAAAAACTTGACAGTACCGTATATATACGGGTATACTAA
AAATATAATTTTGTGAGGCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEKFSFRITKQSGSARVGTIKTPNGSIETPAFIFCATKAAIKAADIERIREAGTQIILSNTYHLMLQPGEDTVAKLGGLH
KMMGWSGPMLTDSGGYQIFSLGHGSVSEEIKGIRQKQKTLIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILV
LDECTPFHVSKEYTRKSMLMSHRWAERSLNKFEKNNDGKQALYGISQGGVYQDLRRESCDFINDLPFFGQAIGGSLGQSK
EQMYDVVSFTMEHLKKDRPTHLLGIGGIVDIFRGVELGVDTFDCVHPTRLARHGGALVKVKNRDSISSKRKEHINLRNQR
FELDDNPIENDCLCFTCRKHSRAYIHHLLKAKELLAYTLVTIHNVFFMNKLMESIRQAILNDRLDQEKNNWISEIPLHCE
LYT

Sequences:

>Translated_403_residues
MEKFSFRITKQSGSARVGTIKTPNGSIETPAFIFCATKAAIKAADIERIREAGTQIILSNTYHLMLQPGEDTVAKLGGLH
KMMGWSGPMLTDSGGYQIFSLGHGSVSEEIKGIRQKQKTLIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILV
LDECTPFHVSKEYTRKSMLMSHRWAERSLNKFEKNNDGKQALYGISQGGVYQDLRRESCDFINDLPFFGQAIGGSLGQSK
EQMYDVVSFTMEHLKKDRPTHLLGIGGIVDIFRGVELGVDTFDCVHPTRLARHGGALVKVKNRDSISSKRKEHINLRNQR
FELDDNPIENDCLCFTCRKHSRAYIHHLLKAKELLAYTLVTIHNVFFMNKLMESIRQAILNDRLDQEKNNWISEIPLHCE
LYT
>Mature_403_residues
MEKFSFRITKQSGSARVGTIKTPNGSIETPAFIFCATKAAIKAADIERIREAGTQIILSNTYHLMLQPGEDTVAKLGGLH
KMMGWSGPMLTDSGGYQIFSLGHGSVSEEIKGIRQKQKTLIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILV
LDECTPFHVSKEYTRKSMLMSHRWAERSLNKFEKNNDGKQALYGISQGGVYQDLRRESCDFINDLPFFGQAIGGSLGQSK
EQMYDVVSFTMEHLKKDRPTHLLGIGGIVDIFRGVELGVDTFDCVHPTRLARHGGALVKVKNRDSISSKRKEHINLRNQR
FELDDNPIENDCLCFTCRKHSRAYIHHLLKAKELLAYTLVTIHNVFFMNKLMESIRQAILNDRLDQEKNNWISEIPLHCE
LYT

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=371, Percent_Identity=37.7358490566038, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI13375872, Length=222, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786607, Length=381, Percent_Identity=39.6325459317585, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17544680, Length=382, Percent_Identity=34.8167539267016, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25146502, Length=235, Percent_Identity=26.8085106382979, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920510, Length=373, Percent_Identity=36.1930294906166, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI21355311, Length=148, Percent_Identity=28.3783783783784, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45645; Mature: 45645

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFSFRITKQSGSARVGTIKTPNGSIETPAFIFCATKAAIKAADIERIREAGTQIILSN
CCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEC
TYHLMLQPGEDTVAKLGGLHKMMGWSGPMLTDSGGYQIFSLGHGSVSEEIKGIRQKQKTL
CEEEEEECCCHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHCCEE
IKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILVLDECTPFHVSKEYTRKSMLM
EEECCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHH
SHRWAERSLNKFEKNNDGKQALYGISQGGVYQDLRRESCDFINDLPFFGQAIGGSLGQSK
HHHHHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHH
EQMYDVVSFTMEHLKKDRPTHLLGIGGIVDIFRGVELGVDTFDCVHPTRLARHGGALVKV
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCHHCCCCCHHHCCHHHHHHCCCCEEEE
KNRDSISSKRKEHINLRNQRFELDDNPIENDCLCFTCRKHSRAYIHHLLKAKELLAYTLV
CCCCCHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
TIHNVFFMNKLMESIRQAILNDRLDQEKNNWISEIPLHCELYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEECC
>Mature Secondary Structure
MEKFSFRITKQSGSARVGTIKTPNGSIETPAFIFCATKAAIKAADIERIREAGTQIILSN
CCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEC
TYHLMLQPGEDTVAKLGGLHKMMGWSGPMLTDSGGYQIFSLGHGSVSEEIKGIRQKQKTL
CEEEEEECCCHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHCCEE
IKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILVLDECTPFHVSKEYTRKSMLM
EEECCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHH
SHRWAERSLNKFEKNNDGKQALYGISQGGVYQDLRRESCDFINDLPFFGQAIGGSLGQSK
HHHHHHHHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHH
EQMYDVVSFTMEHLKKDRPTHLLGIGGIVDIFRGVELGVDTFDCVHPTRLARHGGALVKV
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCHHCCCCCHHHCCHHHHHHCCCCEEEE
KNRDSISSKRKEHINLRNQRFELDDNPIENDCLCFTCRKHSRAYIHHLLKAKELLAYTLV
CCCCCHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
TIHNVFFMNKLMESIRQAILNDRLDQEKNNWISEIPLHCELYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA