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The map label for this gene is nth [H]

Identifier: 190570960

GI number: 190570960

Start: 583788

End: 584426

Strand: Reverse

Name: nth [H]

Synonym: WPa_0545

Alternate gene names: 190570960

Gene position: 584426-583788 (Counterclockwise)

Preceding gene: 190570961

Following gene: 190570957

Centisome position: 39.42

GC content: 35.68

Gene sequence:

>639_bases
ATGGACTCAAAAAAAGTAGAATTAATATTTGAAAAATTTCAACAGTCAAATCCTGCGCCAAAAATAGAGCTAAATTATAC
CAATCATTTTACGTTATTAGTTGCAATAGTTCTGTCAGCGCGGACGACCGATATAAGTGTTAATAAAATTACAAAGGAGC
TATTTAGTATCGCTGATACGCCAGAAAAGATGTTGAATCTTGGGCAAAGTGAGCTGAGAAAATGCATAAGCAGTATTGGT
TTATATAATTCCAAAGCAAAAAATATAATCGGGCTCAGCAAAATATTGATTGAGAGGTACAATAGCAAAGTGCCTACTAA
TTTCGATGATTTGGTATCTTTACCAGGGGTTGGGAGAAAGAGCGCTAATGTGTTCTTAAATTCAGGGCTTGGTATTCCAA
CATTGGCAGTTGATACTCACGTTTTTAGAGTGAGCAATAGAGTTGGGCTTGTGAAAGAAAAAGATGTACTTAAAACAGAG
CAGTCTCTTTTGAATGTAGTGCCAAAAAAATACCTACTTTATGCTCATCATTGGCTAGTTTTACACGGTAGATACATTTG
CAAGGCACAAAAACCTTTGTGTGAAGCGTGCACAATTCATGATTTATGTGAGTTTGAATGCAAGAGGTATAAAGTCTAG

Upstream 100 bases:

>100_bases
AAAAACTGATATGTCTGATGTTAATACTGAAGGACTATCTAATCCTCAAGCAATTGAATTTGCACAGCAAACAGTATAGT
ATAAGCTTTTAGCTCTATGC

Downstream 100 bases:

>100_bases
CACAACACTTCGATATTTCTTACTTTTATCATCCAAGTAGCTGATCTCTATGATGGTGTCAACCGAGTAGCTTACACTGG
GATGGCTTTGTTGCATCGCT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDSKKVELIFEKFQQSNPAPKIELNYTNHFTLLVAIVLSARTTDISVNKITKELFSIADTPEKMLNLGQSELRKCISSIG
LYNSKAKNIIGLSKILIERYNSKVPTNFDDLVSLPGVGRKSANVFLNSGLGIPTLAVDTHVFRVSNRVGLVKEKDVLKTE
QSLLNVVPKKYLLYAHHWLVLHGRYICKAQKPLCEACTIHDLCEFECKRYKV

Sequences:

>Translated_212_residues
MDSKKVELIFEKFQQSNPAPKIELNYTNHFTLLVAIVLSARTTDISVNKITKELFSIADTPEKMLNLGQSELRKCISSIG
LYNSKAKNIIGLSKILIERYNSKVPTNFDDLVSLPGVGRKSANVFLNSGLGIPTLAVDTHVFRVSNRVGLVKEKDVLKTE
QSLLNVVPKKYLLYAHHWLVLHGRYICKAQKPLCEACTIHDLCEFECKRYKV
>Mature_212_residues
MDSKKVELIFEKFQQSNPAPKIELNYTNHFTLLVAIVLSARTTDISVNKITKELFSIADTPEKMLNLGQSELRKCISSIG
LYNSKAKNIIGLSKILIERYNSKVPTNFDDLVSLPGVGRKSANVFLNSGLGIPTLAVDTHVFRVSNRVGLVKEKDVLKTE
QSLLNVVPKKYLLYAHHWLVLHGRYICKAQKPLCEACTIHDLCEFECKRYKV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=205, Percent_Identity=29.7560975609756, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=50, Blast_Score=223, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=33.7016574585635, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=175, Percent_Identity=31.4285714285714, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=33.8888888888889, Blast_Score=97, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23935; Mature: 23935

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSKKVELIFEKFQQSNPAPKIELNYTNHFTLLVAIVLSARTTDISVNKITKELFSIADT
CCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
PEKMLNLGQSELRKCISSIGLYNSKAKNIIGLSKILIERYNSKVPTNFDDLVSLPGVGRK
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
SANVFLNSGLGIPTLAVDTHVFRVSNRVGLVKEKDVLKTEQSLLNVVPKKYLLYAHHWLV
CCEEEEECCCCCCEEHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHGRYICKAQKPLCEACTIHDLCEFECKRYKV
HHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSKKVELIFEKFQQSNPAPKIELNYTNHFTLLVAIVLSARTTDISVNKITKELFSIADT
CCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
PEKMLNLGQSELRKCISSIGLYNSKAKNIIGLSKILIERYNSKVPTNFDDLVSLPGVGRK
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
SANVFLNSGLGIPTLAVDTHVFRVSNRVGLVKEKDVLKTEQSLLNVVPKKYLLYAHHWLV
CCEEEEECCCCCCEEHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHGRYICKAQKPLCEACTIHDLCEFECKRYKV
HHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]