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The map label for this gene is ispB [H]

Identifier: 190570943

GI number: 190570943

Start: 562993

End: 563979

Strand: Reverse

Name: ispB [H]

Synonym: WPa_0524

Alternate gene names: 190570943

Gene position: 563979-562993 (Counterclockwise)

Preceding gene: 190570944

Following gene: 190570942

Centisome position: 38.04

GC content: 33.74

Gene sequence:

>987_bases
ATGCTAAACACTGATTTAACAAAAAGTAGTAAATTAGATGATATTGTATCTTCTGATTTATTGGCTATGAATGACTTTAT
CTTCAAAAATGTCAATGATGAAGGTACTAAGCTTGCTACCGATATTACATCTCATCTTATTAATTCAGGAGGAAAAAAGA
TAAGGCCTAAGCTCATTTTTATTATGTGTAAAATGCTGAATTACTCTGGAGAGAGTAGGGTCAACATTGCTGCATCGGTA
GAATTTATACACAACGCTACTTTACTTCATGATGATGTGCTTGATGAAAGTAAGGCACGCCATGGAGTTGCAACAGCAAA
TAAAATTTGGGGGAATAAATCAAGCATTTTAGTTGGTGATCTATTATTGACCTTAGCATTTAGATGGCTTATAGAATGTG
GGAATTTAAGTGTTCTCTCTATTTTATCTAAGGCATCTAATTTGCTTGTCAAGGGTGAGATAAAGCAGATGACAACGCGT
TTTGACCCTAACACAATTAGGAAAAATTATTTTAATATCATTGGAGAAAAGACAGCATCTTTATTTTCTGCATGCTCCGA
GGCTGCATCAGTAGTATCTGGAGCCACAAGTGACGAAACAGAGAGGCTAAGGAATTTCGGGTTTAATTTTGGCATGGCAT
TCCAAATAATTGATGACGTGCTAGATTATACTGCTAATCAATGCACTTTAGGAAAGCAACTTGGAAAAGATTTCTTTGAA
GGTAAAGTTACTTTACCTTCTATTATAGCATATGAAAAAGGCAGTCCATCAGAACAAAAATTCTGGGAGAAATCTTTTTC
TTCAGCTAGACACAATTTTGACCAAGCATTACAATATATTAATCATCATAATGCCATTCATGTTTCTATGGAAGAAGCTA
AACACTATATTAATATGGCGCAAAGTAATATTGATACTTTTTCTGATTCTCTTTATAAAACTGCTTTGATTGACTTTTTA
AATGCAAGCATAGAAAGACAAGGATAG

Upstream 100 bases:

>100_bases
TTCATTAAAAAAGGCATAGTAGATTTAGAAACAGAATCGAAAAAAATAGAAAAGAAAAAAGTTGAGAAAGTACAAGGGCA
AGAAGCATAAGTCCTCTTAA

Downstream 100 bases:

>100_bases
CTAACGCTTGATATTTTTGATTGATTGTTATATATTTATTAAGTTTGTAAGGGTGAATGATATGTCAGATAGTAATAAAG
AGAAAAAAAAGAAATTTGCA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLNTDLTKSSKLDDIVSSDLLAMNDFIFKNVNDEGTKLATDITSHLINSGGKKIRPKLIFIMCKMLNYSGESRVNIAASV
EFIHNATLLHDDVLDESKARHGVATANKIWGNKSSILVGDLLLTLAFRWLIECGNLSVLSILSKASNLLVKGEIKQMTTR
FDPNTIRKNYFNIIGEKTASLFSACSEAASVVSGATSDETERLRNFGFNFGMAFQIIDDVLDYTANQCTLGKQLGKDFFE
GKVTLPSIIAYEKGSPSEQKFWEKSFSSARHNFDQALQYINHHNAIHVSMEEAKHYINMAQSNIDTFSDSLYKTALIDFL
NASIERQG

Sequences:

>Translated_328_residues
MLNTDLTKSSKLDDIVSSDLLAMNDFIFKNVNDEGTKLATDITSHLINSGGKKIRPKLIFIMCKMLNYSGESRVNIAASV
EFIHNATLLHDDVLDESKARHGVATANKIWGNKSSILVGDLLLTLAFRWLIECGNLSVLSILSKASNLLVKGEIKQMTTR
FDPNTIRKNYFNIIGEKTASLFSACSEAASVVSGATSDETERLRNFGFNFGMAFQIIDDVLDYTANQCTLGKQLGKDFFE
GKVTLPSIIAYEKGSPSEQKFWEKSFSSARHNFDQALQYINHHNAIHVSMEEAKHYINMAQSNIDTFSDSLYKTALIDFL
NASIERQG
>Mature_328_residues
MLNTDLTKSSKLDDIVSSDLLAMNDFIFKNVNDEGTKLATDITSHLINSGGKKIRPKLIFIMCKMLNYSGESRVNIAASV
EFIHNATLLHDDVLDESKARHGVATANKIWGNKSSILVGDLLLTLAFRWLIECGNLSVLSILSKASNLLVKGEIKQMTTR
FDPNTIRKNYFNIIGEKTASLFSACSEAASVVSGATSDETERLRNFGFNFGMAFQIIDDVLDYTANQCTLGKQLGKDFFE
GKVTLPSIIAYEKGSPSEQKFWEKSFSSARHNFDQALQYINHHNAIHVSMEEAKHYINMAQSNIDTFSDSLYKTALIDFL
NASIERQG

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=286, Percent_Identity=32.1678321678322, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1789578, Length=318, Percent_Identity=34.9056603773585, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1786623, Length=243, Percent_Identity=27.9835390946502, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=242, Percent_Identity=34.297520661157, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=294, Percent_Identity=29.2517006802721, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325188, Length=252, Percent_Identity=25, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24651612, Length=283, Percent_Identity=29.3286219081272, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36518; Mature: 36518

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTDLTKSSKLDDIVSSDLLAMNDFIFKNVNDEGTKLATDITSHLINSGGKKIRPKLIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
IMCKMLNYSGESRVNIAASVEFIHNATLLHDDVLDESKARHGVATANKIWGNKSSILVGD
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHH
LLLTLAFRWLIECGNLSVLSILSKASNLLVKGEIKQMTTRFDPNTIRKNYFNIIGEKTAS
HHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LFSACSEAASVVSGATSDETERLRNFGFNFGMAFQIIDDVLDYTANQCTLGKQLGKDFFE
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCHHHHC
GKVTLPSIIAYEKGSPSEQKFWEKSFSSARHNFDQALQYINHHNAIHVSMEEAKHYINMA
CCCCCCHHEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEHHHHHHHHHHH
QSNIDTFSDSLYKTALIDFLNASIERQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNTDLTKSSKLDDIVSSDLLAMNDFIFKNVNDEGTKLATDITSHLINSGGKKIRPKLIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
IMCKMLNYSGESRVNIAASVEFIHNATLLHDDVLDESKARHGVATANKIWGNKSSILVGD
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHH
LLLTLAFRWLIECGNLSVLSILSKASNLLVKGEIKQMTTRFDPNTIRKNYFNIIGEKTAS
HHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LFSACSEAASVVSGATSDETERLRNFGFNFGMAFQIIDDVLDYTANQCTLGKQLGKDFFE
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCHHHHC
GKVTLPSIIAYEKGSPSEQKFWEKSFSSARHNFDQALQYINHHNAIHVSMEEAKHYINMA
CCCCCCHHEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEHHHHHHHHHHH
QSNIDTFSDSLYKTALIDFLNASIERQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]