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The map label for this gene is grpE

Identifier: 190570942

GI number: 190570942

Start: 562371

End: 562931

Strand: Reverse

Name: grpE

Synonym: WPa_0523

Alternate gene names: 190570942

Gene position: 562931-562371 (Counterclockwise)

Preceding gene: 190570943

Following gene: 190570941

Centisome position: 37.97

GC content: 34.4

Gene sequence:

>561_bases
ATGTCAGATAGTAATAAAGAGAAAAAAAAGAAATTTGCAGATATGGTCAGTAAACGAAAAGGGGATGATCAAGAAGATCA
GCAAACAGGTGATTTAAGTGAAGAGCTTAATATATTAAAAGAACGTGCAGTTCAGCTTGAAGATCATTTACGTCGCGCTG
TTGCGGATAATGAAAACGTTAAACGTATAATGCAAAAGCAAATTAGCGATGCAAGTGATTATGCAGTCACAAAATTTGCA
CGTGATATGATCGACTCTTGTGACAATTTAAAAAAAGCAATGGAAAACTTGAAAGATGGTGATCCTATTCATGAAGGAAT
AAAAGTGGCGCACCAAAAGATTGTGAGTGATCTAAAAAAACATGGAATAGAAGAAATAGATCCAATAGGTAACTCTTTTG
ACAGCAATTTACATCAAGCTGTTGTGGAAAGAGAAGATAATGAAAAAGAGCCTGGCACTATTGTAGAAGTACTACAAACT
GGTTATACCATTAAAAATAGGTTGCTTCGTCCTGCAATGGTTATTCTTTCTAAAAAATCTGCTGATTGCGAAAGTAACTA
A

Upstream 100 bases:

>100_bases
ATTGACTTTTTAAATGCAAGCATAGAAAGACAAGGATAGCTAACGCTTGATATTTTTGATTGATTGTTATATATTTATTA
AGTTTGTAAGGGTGAATGAT

Downstream 100 bases:

>100_bases
TAAAACACTCAAGGTATGGAAGAAGTTGTCTTCTCAGGTATTCAGCCAAGTGGAGTATTACACTTGGGCAATTATCTTGG
TGCAATTAAGCAGTGGATAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSDSNKEKKKKFADMVSKRKGDDQEDQQTGDLSEELNILKERAVQLEDHLRRAVADNENVKRIMQKQISDASDYAVTKFA
RDMIDSCDNLKKAMENLKDGDPIHEGIKVAHQKIVSDLKKHGIEEIDPIGNSFDSNLHQAVVEREDNEKEPGTIVEVLQT
GYTIKNRLLRPAMVILSKKSADCESN

Sequences:

>Translated_186_residues
MSDSNKEKKKKFADMVSKRKGDDQEDQQTGDLSEELNILKERAVQLEDHLRRAVADNENVKRIMQKQISDASDYAVTKFA
RDMIDSCDNLKKAMENLKDGDPIHEGIKVAHQKIVSDLKKHGIEEIDPIGNSFDSNLHQAVVEREDNEKEPGTIVEVLQT
GYTIKNRLLRPAMVILSKKSADCESN
>Mature_185_residues
SDSNKEKKKKFADMVSKRKGDDQEDQQTGDLSEELNILKERAVQLEDHLRRAVADNENVKRIMQKQISDASDYAVTKFAR
DMIDSCDNLKKAMENLKDGDPIHEGIKVAHQKIVSDLKKHGIEEIDPIGNSFDSNLHQAVVEREDNEKEPGTIVEVLQTG
YTIKNRLLRPAMVILSKKSADCESN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=173, Percent_Identity=32.3699421965318, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI40255109, Length=173, Percent_Identity=28.3236994219653, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788967, Length=163, Percent_Identity=28.8343558282209, Blast_Score=79, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=32.0754716981132, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=163, Percent_Identity=35.5828220858896, Blast_Score=118, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24653432, Length=155, Percent_Identity=35.4838709677419, Blast_Score=113, Evalue=6e-26,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_WOLPP (B3CPX8)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975300.1
- EnsemblBacteria:   EBWOLT00000000216
- GeneID:   6385190
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0523
- GeneTree:   EBGT00050000031135
- HOGENOM:   HBG732630
- OMA:   MEKYKRA
- ProtClustDB:   CLSK739959
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21063; Mature: 20932

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSNKEKKKKFADMVSKRKGDDQEDQQTGDLSEELNILKERAVQLEDHLRRAVADNENV
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KRIMQKQISDASDYAVTKFARDMIDSCDNLKKAMENLKDGDPIHEGIKVAHQKIVSDLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
HGIEEIDPIGNSFDSNLHQAVVEREDNEKEPGTIVEVLQTGYTIKNRLLRPAMVILSKKS
CCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
ADCESN
CCCCCC
>Mature Secondary Structure 
SDSNKEKKKKFADMVSKRKGDDQEDQQTGDLSEELNILKERAVQLEDHLRRAVADNENV
CCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KRIMQKQISDASDYAVTKFARDMIDSCDNLKKAMENLKDGDPIHEGIKVAHQKIVSDLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
HGIEEIDPIGNSFDSNLHQAVVEREDNEKEPGTIVEVLQTGYTIKNRLLRPAMVILSKKS
CCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
ADCESN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA