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The map label for this gene is lepA [H]

Identifier: 190570916

GI number: 190570916

Start: 533593

End: 535389

Strand: Direct

Name: lepA [H]

Synonym: WPa_0496

Alternate gene names: 190570916

Gene position: 533593-535389 (Clockwise)

Preceding gene: 190570915

Following gene: 190570919

Centisome position: 35.99

GC content: 37.34

Gene sequence:

>1797_bases
ATGAACAATATAAGAAATTTTGCAATAATAGCACATATAGATCATGGGAAGTCAACTCTTGCTGATCGTTTGATAGAAGA
ATGTAACGGTCTTGAGACAAGGGAAATGACCAATCAGGTACTTGACTCAATGGATATAGAGCGTGAACGCGGAATCACAA
TCAAAGCACAAACGGTAAGACTCAATTACACTGCAAATAATGGTAATAAATATTGCCTCAACCTCATGGACACACCAGGT
CATGTTGACTTTTCATATGAAGTCAGCCGAAGCTTAGCCGCATGTGAAGGCTCACTTTTGGTAGTAGATAGCAGCCAAGG
AGTTGAAGCTCAGACTCTGGCAAATGTCTACAAGGCAATTGACAATAATCATGAGATAATAGTTGTGCTTAATAAAGTCG
ATCTTCCTGCTGCAGATCCAGAAAGGATAAAGCTTCAGATTGAAGAGGTAATCGGAATTGATGCAACTGAGTCAATTTTG
ATATCGGCAAAAACTGGTCTTGGGATAAAGGATGTACTTGAAGCTATAGTGACAAAACTTCCCGCTCCTCAAGGTGATAC
AAATGCTCCACTGCAAGCAATTTTAGTTGATAGTTGGTATGATCCTTACCTAGGAGTAGTAATTTTAGTGCGAGTTAAAA
ATGGAGTACTAAAAAAAGGCATGAGAATCGTTATGATGTCTAATAATGCTACATATCAGGTCGATAATATCGGTATTTTC
ACTCCTAAAAAAGTTATGACTGGTGAACTTTCAGCAGGTGAAGTTGGTTTTATAACTGCTTCAATGAAAGAAGTAGCAGA
CTGCAAAGTAGGAGACACTATTACTGAAGAGAAAAGGCCGTGCAGCAAGGCATTACCTGGCTTTAAAGAAGTACATCCTG
TAGTATTTTGCAGTATTTTTCCCAATAACACAGATGATTTTAAATATTTAAGGGAAGCACTAGAAAAATTACATTTAAAT
GATGCTAGTTTTACATTTGATGCTGAAACGTCAAATGCCCTAGGTTATGGATTTCGTTGCGGTTTCTTAGGAATGCTACA
TCTTGAAGTCATTCAAGAAAGACTCGAAAGAGAATTTGATTTAGATCTAACAGCAACTGCACCGAGTGTTATATATAAAG
TTGAAACACAAAATGGTAAAGTTTTAAATATCCATAACCCAAGCGATATGCCAGACCCTACAAAAATTGAAATAGTGGAA
GAACCGTGGATCACTGCAACTATAATGGTACCTGATCAATATTTAGGAGAGATTCTATCTCTGTGTGAAGAGAGGAGAGG
AGAACAGCAGGATTTATCTTATGTTGGTAATACGACAACAGCACTACTGAAGTATAAATTACCGCTGTCTGAGGTTGTTT
TTGATTTTTACGATAGACTAAAATCAATTTCCAAGGGATATGCAAGTTTAGATTGGGAAATATCCGATTATCAGGAAAGT
CAGATAGATAAATTAAGTTTTTTAGTTAATGGAGAACCTGTGGATGCGTTAGCCTGCATTGTTCATAAAAGCAGGGCAGA
AAAAAGAGGCCGTGAAATATGTGCACGTTTGAAAGACTTGATACCACGCCAGCAATATAAAATCGCGATTCAAGCGGCAG
TAGGCGGAAAAATTATTGCTAGAGAAACGATTAACCCGTATAGAAAAGATGTAACAGCCAAGCTCTATGGTGGAGACGTT
ACACGAAGGATGAAGCTGCTTGAAAAGCAGAAGAAAGGTAAGAAAAGATTGCATTCTGTAGGAAACATAAACATCCCACA
AAATGCTTTTATTGAGGCTTTGAAAATAAATGATTAA

Upstream 100 bases:

>100_bases
ATTGGCAAGGACAGCTTAATGTTTTTAGCAAAGAGAGATATAAGTAATTGCTCTTGTAATACTTAAAATGTAAAATAGAT
CTACTTAAATAAGTAGATCT

Downstream 100 bases:

>100_bases
TATATATTCAAAAACATAAATTTAAAGAACTAAGTGATATTTTCTCAATAAATTTACTGACATTGATATGGGTAAATTTG
AAATAGCTATGTGAATTTGT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLETREMTNQVLDSMDIERERGITIKAQTVRLNYTANNGNKYCLNLMDTPG
HVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDNNHEIIVVLNKVDLPAADPERIKLQIEEVIGIDATESIL
ISAKTGLGIKDVLEAIVTKLPAPQGDTNAPLQAILVDSWYDPYLGVVILVRVKNGVLKKGMRIVMMSNNATYQVDNIGIF
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSKALPGFKEVHPVVFCSIFPNNTDDFKYLREALEKLHLN
DASFTFDAETSNALGYGFRCGFLGMLHLEVIQERLEREFDLDLTATAPSVIYKVETQNGKVLNIHNPSDMPDPTKIEIVE
EPWITATIMVPDQYLGEILSLCEERRGEQQDLSYVGNTTTALLKYKLPLSEVVFDFYDRLKSISKGYASLDWEISDYQES
QIDKLSFLVNGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIARETINPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLHSVGNINIPQNAFIEALKIND

Sequences:

>Translated_598_residues
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLETREMTNQVLDSMDIERERGITIKAQTVRLNYTANNGNKYCLNLMDTPG
HVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDNNHEIIVVLNKVDLPAADPERIKLQIEEVIGIDATESIL
ISAKTGLGIKDVLEAIVTKLPAPQGDTNAPLQAILVDSWYDPYLGVVILVRVKNGVLKKGMRIVMMSNNATYQVDNIGIF
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSKALPGFKEVHPVVFCSIFPNNTDDFKYLREALEKLHLN
DASFTFDAETSNALGYGFRCGFLGMLHLEVIQERLEREFDLDLTATAPSVIYKVETQNGKVLNIHNPSDMPDPTKIEIVE
EPWITATIMVPDQYLGEILSLCEERRGEQQDLSYVGNTTTALLKYKLPLSEVVFDFYDRLKSISKGYASLDWEISDYQES
QIDKLSFLVNGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIARETINPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLHSVGNINIPQNAFIEALKIND
>Mature_598_residues
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLETREMTNQVLDSMDIERERGITIKAQTVRLNYTANNGNKYCLNLMDTPG
HVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDNNHEIIVVLNKVDLPAADPERIKLQIEEVIGIDATESIL
ISAKTGLGIKDVLEAIVTKLPAPQGDTNAPLQAILVDSWYDPYLGVVILVRVKNGVLKKGMRIVMMSNNATYQVDNIGIF
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSKALPGFKEVHPVVFCSIFPNNTDDFKYLREALEKLHLN
DASFTFDAETSNALGYGFRCGFLGMLHLEVIQERLEREFDLDLTATAPSVIYKVETQNGKVLNIHNPSDMPDPTKIEIVE
EPWITATIMVPDQYLGEILSLCEERRGEQQDLSYVGNTTTALLKYKLPLSEVVFDFYDRLKSISKGYASLDWEISDYQES
QIDKLSFLVNGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIARETINPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLHSVGNINIPQNAFIEALKIND

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=603, Percent_Identity=47.7611940298507, Blast_Score=597, Evalue=1e-171,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=44.0298507462687, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=38.1944444444444, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=43.2432432432432, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=43.2432432432432, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=43.2432432432432, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI25306283, Length=178, Percent_Identity=38.2022471910112, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI18390331, Length=182, Percent_Identity=32.967032967033, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI19923640, Length=178, Percent_Identity=38.2022471910112, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI25306287, Length=178, Percent_Identity=38.2022471910112, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI217272892, Length=161, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=161, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI53729339, Length=227, Percent_Identity=29.0748898678414, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI53729337, Length=227, Percent_Identity=29.0748898678414, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI34147630, Length=259, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=37.1134020618557, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=56.5436241610738, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=523, Percent_Identity=28.6806883365201, Blast_Score=179, Evalue=7e-46,
Organism=Escherichia coli, GI1790835, Length=158, Percent_Identity=32.9113924050633, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789738, Length=165, Percent_Identity=33.9393939393939, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789559, Length=270, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=40.2936378466558, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17556745, Length=461, Percent_Identity=24.5119305856833, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=35.6643356643357, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=37.3134328358209, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=37.3134328358209, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=219, Percent_Identity=30.5936073059361, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=163, Percent_Identity=32.5153374233129, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=262, Percent_Identity=25.1908396946565, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=232, Percent_Identity=28.448275862069, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=46.1794019933555, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=38.3561643835616, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=38.3561643835616, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=34.4262295081967, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=138, Percent_Identity=34.7826086956522, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=317, Percent_Identity=25.8675078864353, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=44.4444444444444, Blast_Score=533, Evalue=1e-151,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=42.4460431654676, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24582462, Length=158, Percent_Identity=34.8101265822785, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357743, Length=180, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI221458488, Length=144, Percent_Identity=39.5833333333333, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=36.4864864864865, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=36.4864864864865, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=36.4864864864865, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=240, Percent_Identity=30, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281363316, Length=282, Percent_Identity=24.822695035461, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17864358, Length=282, Percent_Identity=24.822695035461, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19921738, Length=256, Percent_Identity=25.78125, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66690; Mature: 66690

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLETREMTNQVLDSMDIERERGITIKAQTVR
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYTANNGNKYCLNLMDTPGHVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
DNNHEIIVVLNKVDLPAADPERIKLQIEEVIGIDATESILISAKTGLGIKDVLEAIVTKL
CCCCEEEEEEEECCCCCCCCHHEEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHC
PAPQGDTNAPLQAILVDSWYDPYLGVVILVRVKNGVLKKGMRIVMMSNNATYQVDNIGIF
CCCCCCCCCCEEEEEECCCCCCHHEEEEEEEECCCHHHCCCEEEEEECCCEEEECCEEEE
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSKALPGFKEVHPVVFCSIF
CCCHHEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCEEEEEEEC
PNNTDDFKYLREALEKLHLNDASFTFDAETSNALGYGFRCGFLGMLHLEVIQERLEREFD
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LDLTATAPSVIYKVETQNGKVLNIHNPSDMPDPTKIEIVEEPWITATIMVPDQYLGEILS
CEEEECCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHH
LCEERRGEQQDLSYVGNTTTALLKYKLPLSEVVFDFYDRLKSISKGYASLDWEISDYQES
HHHHHCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCEECCCCHH
QIDKLSFLVNGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIA
HHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEE
RETINPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKRLHSVGNINIPQNAFIEALKIND
ECCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCC
>Mature Secondary Structure
MNNIRNFAIIAHIDHGKSTLADRLIEECNGLETREMTNQVLDSMDIERERGITIKAQTVR
CCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYTANNGNKYCLNLMDTPGHVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
DNNHEIIVVLNKVDLPAADPERIKLQIEEVIGIDATESILISAKTGLGIKDVLEAIVTKL
CCCCEEEEEEEECCCCCCCCHHEEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHC
PAPQGDTNAPLQAILVDSWYDPYLGVVILVRVKNGVLKKGMRIVMMSNNATYQVDNIGIF
CCCCCCCCCCEEEEEECCCCCCHHEEEEEEEECCCHHHCCCEEEEEECCCEEEECCEEEE
TPKKVMTGELSAGEVGFITASMKEVADCKVGDTITEEKRPCSKALPGFKEVHPVVFCSIF
CCCHHEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHCCEEEEEEEC
PNNTDDFKYLREALEKLHLNDASFTFDAETSNALGYGFRCGFLGMLHLEVIQERLEREFD
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LDLTATAPSVIYKVETQNGKVLNIHNPSDMPDPTKIEIVEEPWITATIMVPDQYLGEILS
CEEEECCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHH
LCEERRGEQQDLSYVGNTTTALLKYKLPLSEVVFDFYDRLKSISKGYASLDWEISDYQES
HHHHHCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCEECCCCHH
QIDKLSFLVNGEPVDALACIVHKSRAEKRGREICARLKDLIPRQQYKIAIQAAVGGKIIA
HHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEE
RETINPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKRLHSVGNINIPQNAFIEALKIND
ECCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA