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The map label for this gene is ribF [H]

Identifier: 190570919

GI number: 190570919

Start: 537036

End: 537968

Strand: Direct

Name: ribF [H]

Synonym: WPa_0499

Alternate gene names: 190570919

Gene position: 537036-537968 (Clockwise)

Preceding gene: 190570916

Following gene: 190570920

Centisome position: 36.23

GC content: 32.05

Gene sequence:

>933_bases
ATGAAAATTATTTACAACTGTGAGCAAGGGATAAAAGATAATGTGGCACTAACTTTCGGAAATTTCGATGGCGTTCATTT
AGGACATAAGTTTGCAATTTCCAATCTAAAGAAGGTAGCACAAGAAAGAGGATTACCCTCTGCAGTTTTAACTTTTGAGC
CTCACCCATCAACTGTTTTATTTAGTAGGAACAATTTTAGGTTAATAGACCAAGAACAAAAAAAGGAACTAATCAGCAGC
TATGGTATAGATTACTTGTATATTATTAATTTTAATAGAGGTTTTTCTGAGGTTAGCTGCGATGACTTCATTGGTAAGAT
TTTGGTAAAGAAGTATGGTGCTAAACATATAACTGTTGGAGAAAGTTGTACTTTTGGTCACAAACGATTGGGTAACATAT
TAACTCTAGAAAAATATTCTCAGGTGTATGAATATTCTTTAACTAAATTAGAGCCATTGATAATCGATAGAAAAATTTGC
TCTTCTTCCTCAATCAGGGAGTGTTTACAAAAAGGCGAAATAGAAATTGCTAATAAATTGCTTGGTAGGTCTTATCAAGT
TTCTGGTATTGTAACAAAAGGTGCATGTAGAGGTAGAGAAATAGGGTTTCCAACCATAAACATTCCGATAGAAGATTACA
TGATAAAACCTAAGCTTGGTACATATTACGCCAAAGTTGCATTTTCTGAAAATAGTTCAAGTTGGCTATATGGAGTAGTC
AACATTGGTATGAGACCTACATTTAAGGACTTAAAAAAACCTATAGTAGAAATGCATATATTTGACTTCAACGAAAATGT
ATACAATTTTAAGGTTGACATACAACTTTTAAAATTTATTAGGGCAGAAAAAAAATTCCATAGTATTAACGAGTTAACAA
AACAGATAGACCGTGATATAATGAAGGCTTACCAGTTAAAGATAAACTTATGA

Upstream 100 bases:

>100_bases
TATATTACAACTTTTCCTTTAGTGTTTTTCACGATTTATCTCCATAGTTCATAAAATACAACTATTATATATAAAAAATA
TATATAAAATATCAAATTGT

Downstream 100 bases:

>100_bases
AAAGGATATGTTTAGTTATTATATTGATTATAGTATCATTCGATCAAACAAGTAAATTGTATATAAACTCATTGATTGAT
GAGGGGGAGTCAATCGAGAT

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKIIYNCEQGIKDNVALTFGNFDGVHLGHKFAISNLKKVAQERGLPSAVLTFEPHPSTVLFSRNNFRLIDQEQKKELISS
YGIDYLYIINFNRGFSEVSCDDFIGKILVKKYGAKHITVGESCTFGHKRLGNILTLEKYSQVYEYSLTKLEPLIIDRKIC
SSSSIRECLQKGEIEIANKLLGRSYQVSGIVTKGACRGREIGFPTINIPIEDYMIKPKLGTYYAKVAFSENSSSWLYGVV
NIGMRPTFKDLKKPIVEMHIFDFNENVYNFKVDIQLLKFIRAEKKFHSINELTKQIDRDIMKAYQLKINL

Sequences:

>Translated_310_residues
MKIIYNCEQGIKDNVALTFGNFDGVHLGHKFAISNLKKVAQERGLPSAVLTFEPHPSTVLFSRNNFRLIDQEQKKELISS
YGIDYLYIINFNRGFSEVSCDDFIGKILVKKYGAKHITVGESCTFGHKRLGNILTLEKYSQVYEYSLTKLEPLIIDRKIC
SSSSIRECLQKGEIEIANKLLGRSYQVSGIVTKGACRGREIGFPTINIPIEDYMIKPKLGTYYAKVAFSENSSSWLYGVV
NIGMRPTFKDLKKPIVEMHIFDFNENVYNFKVDIQLLKFIRAEKKFHSINELTKQIDRDIMKAYQLKINL
>Mature_310_residues
MKIIYNCEQGIKDNVALTFGNFDGVHLGHKFAISNLKKVAQERGLPSAVLTFEPHPSTVLFSRNNFRLIDQEQKKELISS
YGIDYLYIINFNRGFSEVSCDDFIGKILVKKYGAKHITVGESCTFGHKRLGNILTLEKYSQVYEYSLTKLEPLIIDRKIC
SSSSIRECLQKGEIEIANKLLGRSYQVSGIVTKGACRGREIGFPTINIPIEDYMIKPKLGTYYAKVAFSENSSSWLYGVV
NIGMRPTFKDLKKPIVEMHIFDFNENVYNFKVDIQLLKFIRAEKKFHSINELTKQIDRDIMKAYQLKINL

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=304, Percent_Identity=33.5526315789474, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 35525; Mature: 35525

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIYNCEQGIKDNVALTFGNFDGVHLGHKFAISNLKKVAQERGLPSAVLTFEPHPSTVL
CEEEEECCCCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
FSRNNFRLIDQEQKKELISSYGIDYLYIINFNRGFSEVSCDDFIGKILVKKYGAKHITVG
EECCCEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEC
ESCTFGHKRLGNILTLEKYSQVYEYSLTKLEPLIIDRKICSSSSIRECLQKGEIEIANKL
CCCCCCHHHHCCEEEHHHHHHHHHHHHHHCCHHEEEHHHCCCHHHHHHHHCCCHHHHHHH
LGRSYQVSGIVTKGACRGREIGFPTINIPIEDYMIKPKLGTYYAKVAFSENSSSWLYGVV
HCCCEEEEEEEECCCCCCCCCCCCEEECCHHHEEECCCCCCEEEEEEECCCCCCEEEEEE
NIGMRPTFKDLKKPIVEMHIFDFNENVYNFKVDIQLLKFIRAEKKFHSINELTKQIDRDI
ECCCCCCHHHHHHHHHEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKAYQLKINL
HHHHEEEECC
>Mature Secondary Structure
MKIIYNCEQGIKDNVALTFGNFDGVHLGHKFAISNLKKVAQERGLPSAVLTFEPHPSTVL
CEEEEECCCCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
FSRNNFRLIDQEQKKELISSYGIDYLYIINFNRGFSEVSCDDFIGKILVKKYGAKHITVG
EECCCEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEC
ESCTFGHKRLGNILTLEKYSQVYEYSLTKLEPLIIDRKICSSSSIRECLQKGEIEIANKL
CCCCCCHHHHCCEEEHHHHHHHHHHHHHHCCHHEEEHHHCCCHHHHHHHHCCCHHHHHHH
LGRSYQVSGIVTKGACRGREIGFPTINIPIEDYMIKPKLGTYYAKVAFSENSSSWLYGVV
HCCCEEEEEEEECCCCCCCCCCCCEEECCHHHEEECCCCCCEEEEEEECCCCCCEEEEEE
NIGMRPTFKDLKKPIVEMHIFDFNENVYNFKVDIQLLKFIRAEKKFHSINELTKQIDRDI
ECCCCCCHHHHHHHHHEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKAYQLKINL
HHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]