Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 190570818

GI number: 190570818

Start: 409770

End: 411572

Strand: Reverse

Name: aspS [H]

Synonym: WPa_0387

Alternate gene names: 190570818

Gene position: 411572-409770 (Counterclockwise)

Preceding gene: 190570820

Following gene: 190570816

Centisome position: 27.76

GC content: 36.88

Gene sequence:

>1803_bases
ATGAACTGTTATAAAACTCACGAGTGTAATGAATTAAGGAAGAATGATGTAGAAAAGGAAGTTACTCTCTCTGGGTGGCT
GTACCGTAAACGTGACCATGGTAACCTAATCTTTGTTGATTTAAGAGATTTTCATGGGATTACTCAGTTGGTATTCAATA
ACGATAAAGATTTTTTTGATGAAATTTCGAATTTAAAATCAGAAAGTGTGATTACTGTCACAGGAATAGTCAAAGCTAGA
ACTGAAGATACGGTAAATAGCTCTATTTCCACAGGAGAAATTGAAGTTATAGTTAGTAATTTACAAGTTGAATCTGAAGT
TGAGTTCCACTGTGATGAAGAAATAGCAAAAGAAGAAAGGAGTATATTAGCAAGTATCGCCGGTGAGCAAGAATACCCAG
AAAACATGAGATTTAAATATCGTTTTCTTGATTTAAGACGTGAAAAGGTTCGTAATAACATTATTCTACGTTCACAGATC
ATTTCAGAGCTCAGGAGGCTCATGATAGAGCAAGGATTCTTAGATATTCAAACTCCCATACTTACTGCCTCTTCTCCTGA
AGGAGCACGTGATTATTTAGTGCCAAGCAGACTAAATCCTGGTAAATTCTATGCATTACCACAAGCTCCACAGATTTTTA
AGCAGTTGCTTATGGTTTCAGGGTTTGATAAATATTTTCAAATTGCACCTTGTTTTCGTGATGAGGACGCAAGGGCTGAC
CGTTCTCCTGGGGAGTTTTATCAGCTAGATCTTGAAATGTCTTTTGTAACTCAAGAAGATATATTTCAGGTTATTGAATC
TACCTTATATAAAGTGTTTGCCAAATTTTCTCGCAAATCAGTTGATAAAGATTTTCCACGTATTACATACAAAGAAGCAA
TGCTCAAATATGGATCTGATAAGCCAGATCTGCGTAATCCATTATTAATCAGCGATGTTACAGAGATTTTCCGTGATTCA
GAGTTCAATATTTTCAAAAGCAATATCGAGCGAGGTATGGTAGTCAGAGCCATCCCTGCTCCTAAAACAGCAGAGGAACC
ACGCAGTTTTTTTGATAAAAAAATAGAACATGCGCAAAAAGAATTCGGTGCTAAAGGTCTTGGATATATAACGTTTGATA
AAGATGGCATCGCAAAAGGGCCAATTGCTAAATTCCTCGATGACAATAGGCTAAATTCCATAAAAGAAATAACGAATGTA
AAGCCCGGAGATAGTGTATTTTTTGCTTCTGATAAGGAAAATGAAGCAGCAACAATTGCAGGGAAAGTGCGCACTCTTTT
AGGTTCAGAATTGGGATTAATAGATGACGATATCTTCAAATTCTGTTGGATTATTGATTTTCCATATTTTGTATATGACG
ATAAGAGCAAAAAAATAGATTTCTTTCACAACCCATTCTCTATGCCACATGGTGGCTTGAAAGATTTAGAAGAAAAAGAT
CCGCTGGATATCCTTGCTTACCAATATGATCTTGTTTGCAATGGAATAGAGCTGTCAAGTGGAGCAATTCGTAATAACAA
ACTGAATATTATGTACAGAGCTTTTGCCATTGCAGGCTACAGTAAAGAGGAAGTTGATACAAAATTTGGCGCGCTTGTGC
GTGCATTCAGGTTTGGAGCGCCACCTCATGGTGGAATAGCGCCAGGGGTTGATAGAATGGTTATGCTACTTGCAGATGAG
CCGAATATTCGTGAAGTAATCTGTTTTCCTATGAATCAGCAAGGAGAAGATGTTCTAATGGGCGCTCCTTCTAAAGTAGA
TGATAAGCATTTACGTGAGTTGTCTTTAAAGGTTGTTGAATAA

Upstream 100 bases:

>100_bases
ATATCTAAATCAGAATTGCTTGTAAAGGATCTTTAGATATTATCTTGCCCAAAGGTTAATTTATTTTATAATCTTTTTGT
ATTCTAGAAATTTCAATTAA

Downstream 100 bases:

>100_bases
AGTTGTATATAACTTGAGTTTAGGTGGGGAAAAAACAAGAATATTGAATTCTGTTAAAGTAGTGCTAGGCCCTAGCACTA
CTTTATAGTTTTAGGATATC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MNCYKTHECNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFHGITQLVFNNDKDFFDEISNLKSESVITVTGIVKAR
TEDTVNSSISTGEIEVIVSNLQVESEVEFHCDEEIAKEERSILASIAGEQEYPENMRFKYRFLDLRREKVRNNIILRSQI
ISELRRLMIEQGFLDIQTPILTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
RSPGEFYQLDLEMSFVTQEDIFQVIESTLYKVFAKFSRKSVDKDFPRITYKEAMLKYGSDKPDLRNPLLISDVTEIFRDS
EFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQKEFGAKGLGYITFDKDGIAKGPIAKFLDDNRLNSIKEITNV
KPGDSVFFASDKENEAATIAGKVRTLLGSELGLIDDDIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEEKD
PLDILAYQYDLVCNGIELSSGAIRNNKLNIMYRAFAIAGYSKEEVDTKFGALVRAFRFGAPPHGGIAPGVDRMVMLLADE
PNIREVICFPMNQQGEDVLMGAPSKVDDKHLRELSLKVVE

Sequences:

>Translated_600_residues
MNCYKTHECNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFHGITQLVFNNDKDFFDEISNLKSESVITVTGIVKAR
TEDTVNSSISTGEIEVIVSNLQVESEVEFHCDEEIAKEERSILASIAGEQEYPENMRFKYRFLDLRREKVRNNIILRSQI
ISELRRLMIEQGFLDIQTPILTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
RSPGEFYQLDLEMSFVTQEDIFQVIESTLYKVFAKFSRKSVDKDFPRITYKEAMLKYGSDKPDLRNPLLISDVTEIFRDS
EFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQKEFGAKGLGYITFDKDGIAKGPIAKFLDDNRLNSIKEITNV
KPGDSVFFASDKENEAATIAGKVRTLLGSELGLIDDDIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEEKD
PLDILAYQYDLVCNGIELSSGAIRNNKLNIMYRAFAIAGYSKEEVDTKFGALVRAFRFGAPPHGGIAPGVDRMVMLLADE
PNIREVICFPMNQQGEDVLMGAPSKVDDKHLRELSLKVVE
>Mature_600_residues
MNCYKTHECNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFHGITQLVFNNDKDFFDEISNLKSESVITVTGIVKAR
TEDTVNSSISTGEIEVIVSNLQVESEVEFHCDEEIAKEERSILASIAGEQEYPENMRFKYRFLDLRREKVRNNIILRSQI
ISELRRLMIEQGFLDIQTPILTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
RSPGEFYQLDLEMSFVTQEDIFQVIESTLYKVFAKFSRKSVDKDFPRITYKEAMLKYGSDKPDLRNPLLISDVTEIFRDS
EFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQKEFGAKGLGYITFDKDGIAKGPIAKFLDDNRLNSIKEITNV
KPGDSVFFASDKENEAATIAGKVRTLLGSELGLIDDDIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEEKD
PLDILAYQYDLVCNGIELSSGAIRNNKLNIMYRAFAIAGYSKEEVDTKFGALVRAFRFGAPPHGGIAPGVDRMVMLLADE
PNIREVICFPMNQQGEDVLMGAPSKVDDKHLRELSLKVVE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=609, Percent_Identity=33.6617405582923, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI45439306, Length=368, Percent_Identity=25.8152173913043, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788173, Length=606, Percent_Identity=41.5841584158416, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1789256, Length=306, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1790571, Length=309, Percent_Identity=26.2135922330097, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787161, Length=304, Percent_Identity=25.6578947368421, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=636, Percent_Identity=31.4465408805031, Blast_Score=273, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17551876, Length=294, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=344, Percent_Identity=23.546511627907, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=344, Percent_Identity=23.546511627907, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=344, Percent_Identity=23.546511627907, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=666, Percent_Identity=32.2822822822823, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323011, Length=309, Percent_Identity=30.0970873786408, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=261, Percent_Identity=24.5210727969349, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=242, Percent_Identity=24.7933884297521, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24584738, Length=622, Percent_Identity=34.2443729903537, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=24.2424242424242, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640849, Length=266, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24640851, Length=266, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68571; Mature: 68571

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCYKTHECNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFHGITQLVFNNDKDFFD
CCCCCCCCHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECHHHCCHHHHHCCCCHHHHH
EISNLKSESVITVTGIVKARTEDTVNSSISTGEIEVIVSNLQVESEVEFHCDEEIAKEER
HHHCCCCCCEEEEEEEEEECCCHHHCCCCCCCEEEEEEECCEECCCCCCCCCHHHHHHHH
SILASIAGEQEYPENMRFKYRFLDLRREKVRNNIILRSQIISELRRLMIEQGFLDIQTPI
HHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCEECCCE
LTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
EECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCC
RSPGEFYQLDLEMSFVTQEDIFQVIESTLYKVFAKFSRKSVDKDFPRITYKEAMLKYGSD
CCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
KPDLRNPLLISDVTEIFRDSEFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQK
CCCCCCCEEHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EFGAKGLGYITFDKDGIAKGPIAKFLDDNRLNSIKEITNVKPGDSVFFASDKENEAATIA
HHCCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHH
GKVRTLLGSELGLIDDDIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEEKD
HHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
PLDILAYQYDLVCNGIELSSGAIRNNKLNIMYRAFAIAGYSKEEVDTKFGALVRAFRFGA
CCEEEEEEEEHEECCEEECCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
PPHGGIAPGVDRMVMLLADEPNIREVICFPMNQQGEDVLMGAPSKVDDKHLRELSLKVVE
CCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNCYKTHECNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFHGITQLVFNNDKDFFD
CCCCCCCCHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECHHHCCHHHHHCCCCHHHHH
EISNLKSESVITVTGIVKARTEDTVNSSISTGEIEVIVSNLQVESEVEFHCDEEIAKEER
HHHCCCCCCEEEEEEEEEECCCHHHCCCCCCCEEEEEEECCEECCCCCCCCCHHHHHHHH
SILASIAGEQEYPENMRFKYRFLDLRREKVRNNIILRSQIISELRRLMIEQGFLDIQTPI
HHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCEECCCE
LTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
EECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCC
RSPGEFYQLDLEMSFVTQEDIFQVIESTLYKVFAKFSRKSVDKDFPRITYKEAMLKYGSD
CCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
KPDLRNPLLISDVTEIFRDSEFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQK
CCCCCCCEEHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EFGAKGLGYITFDKDGIAKGPIAKFLDDNRLNSIKEITNVKPGDSVFFASDKENEAATIA
HHCCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHH
GKVRTLLGSELGLIDDDIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEEKD
HHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
PLDILAYQYDLVCNGIELSSGAIRNNKLNIMYRAFAIAGYSKEEVDTKFGALVRAFRFGA
CCEEEEEEEEHEECCEEECCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
PPHGGIAPGVDRMVMLLADEPNIREVICFPMNQQGEDVLMGAPSKVDDKHLRELSLKVVE
CCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA