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The map label for this gene is prsA [H]

Identifier: 190570677

GI number: 190570677

Start: 236904

End: 237824

Strand: Reverse

Name: prsA [H]

Synonym: WPa_0227

Alternate gene names: 190570677

Gene position: 237824-236904 (Counterclockwise)

Preceding gene: 190570679

Following gene: 190570676

Centisome position: 16.04

GC content: 35.18

Gene sequence:

>921_bases
ATGAAGGTAATAATAGGTAGCGCTAGTAAACAATTAGGAGATTCAATAGCAAGTGGGCTAAATGCTCAGCTATTCCCCAA
TCAGGTGTCAAGATTTGCCGATGGTGAGGTAAATGTAGAAGTAGCAAATGATCTACGTAATCAAGAAGTATATATAGTAC
AATCTCTTTCTTCCCCTGTAAATGATAACCTTATGGAGCTTCTGCTTACAATTGATGCAGCAAAGAGATCTGGAGCCAAG
AGAATAACAGCTATTATTCCTTATTACGGATACAGTAGGCAGGATAGGGTTATTAAAAACAATAATATGCAATCTGCTTT
AAGTGCTAAATTAACTGCAAATCTTATTCAAATAGCTGGTGCAAACAGTGTTGCTGCTATTGATTTGCATTCAAGTCAAA
TTGAAGGTTTTTTTGATATACCAGTTACTAATCTAAGCTGTTTTGAAGTATTTGTTAACTCTATATACAAGGAAAACTTG
GCAATAGTTGCGCCTGATGTTGGAGCAATTGGCAGAGCTCGTGCTTTTGCAAAGATTTTAGAGGAAAAACACAAGTTGGA
TAATGATATTGTTGTAGTAGATAAATATAGAGAAAAAGCAGGTACATCTGAGGTAATGAATGTAATCGGAGAAGTTGCAA
ACAAAAATTGCGTCATTGTTGATGATATAGTTGACTCTGGCGGAACATTATGTAATGCAGCTCTTGCTTTAAAAAATCGA
GGAGCAAAGTCTGTAGTTTCGTGCATTACACATGGAATACTTTCAGGAAATGCAGTTGAGAAAATCTCTTCCTCTTCTCT
GGATAAATTAGTAATTACGGACACCGTATTTCACAAATTTGAAAAAAATGATAAGATAGAGGTTGTTTCAATTGCAAATA
TTTTAATTTGCTTTATGCAAGGAGGTAAAAGTGCCAGCTAG

Upstream 100 bases:

>100_bases
TTCGAGTAACTTTTCCGTTTCTATGAGCTACTTGGCAGCAAGAATAAAGTTTTAATCATTCCATGGTTTCTGTATAATTT
TCAAAACTTAATTGGTATAT

Downstream 100 bases:

>100_bases
ATCAACAGAAGATGTTATCACTTCGTTAATAGAATTTGCAAATAAGAGAAAAATAACAATTACTAAGGAAGAAATGCTTA
AAACTGCACAGCTTGTGAGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKVIIGSASKQLGDSIASGLNAQLFPNQVSRFADGEVNVEVANDLRNQEVYIVQSLSSPVNDNLMELLLTIDAAKRSGAK
RITAIIPYYGYSRQDRVIKNNNMQSALSAKLTANLIQIAGANSVAAIDLHSSQIEGFFDIPVTNLSCFEVFVNSIYKENL
AIVAPDVGAIGRARAFAKILEEKHKLDNDIVVVDKYREKAGTSEVMNVIGEVANKNCVIVDDIVDSGGTLCNAALALKNR
GAKSVVSCITHGILSGNAVEKISSSSLDKLVITDTVFHKFEKNDKIEVVSIANILICFMQGGKSAS

Sequences:

>Translated_306_residues
MKVIIGSASKQLGDSIASGLNAQLFPNQVSRFADGEVNVEVANDLRNQEVYIVQSLSSPVNDNLMELLLTIDAAKRSGAK
RITAIIPYYGYSRQDRVIKNNNMQSALSAKLTANLIQIAGANSVAAIDLHSSQIEGFFDIPVTNLSCFEVFVNSIYKENL
AIVAPDVGAIGRARAFAKILEEKHKLDNDIVVVDKYREKAGTSEVMNVIGEVANKNCVIVDDIVDSGGTLCNAALALKNR
GAKSVVSCITHGILSGNAVEKISSSSLDKLVITDTVFHKFEKNDKIEVVSIANILICFMQGGKSAS
>Mature_306_residues
MKVIIGSASKQLGDSIASGLNAQLFPNQVSRFADGEVNVEVANDLRNQEVYIVQSLSSPVNDNLMELLLTIDAAKRSGAK
RITAIIPYYGYSRQDRVIKNNNMQSALSAKLTANLIQIAGANSVAAIDLHSSQIEGFFDIPVTNLSCFEVFVNSIYKENL
AIVAPDVGAIGRARAFAKILEEKHKLDNDIVVVDKYREKAGTSEVMNVIGEVANKNCVIVDDIVDSGGTLCNAALALKNR
GAKSVVSCITHGILSGNAVEKISSSSLDKLVITDTVFHKFEKNDKIEVVSIANILICFMQGGKSAS

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=302, Percent_Identity=37.7483443708609, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI84875539, Length=298, Percent_Identity=37.5838926174497, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4506129, Length=298, Percent_Identity=37.5838926174497, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI28557709, Length=302, Percent_Identity=37.4172185430464, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4506133, Length=321, Percent_Identity=32.0872274143302, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI194018537, Length=320, Percent_Identity=32.5, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=31.7241379310345, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=31.7241379310345, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=31.7241379310345, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=31.7241379310345, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787458, Length=279, Percent_Identity=43.7275985663082, Blast_Score=255, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI25149168, Length=303, Percent_Identity=36.3036303630363, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17554702, Length=303, Percent_Identity=36.3036303630363, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI71989924, Length=303, Percent_Identity=36.3036303630363, Blast_Score=193, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17554704, Length=301, Percent_Identity=36.2126245847176, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17570245, Length=330, Percent_Identity=30.6060606060606, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321776, Length=317, Percent_Identity=37.8548895899054, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6319403, Length=283, Percent_Identity=38.86925795053, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6320946, Length=282, Percent_Identity=37.9432624113475, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322667, Length=203, Percent_Identity=33.0049261083744, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324511, Length=92, Percent_Identity=41.304347826087, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=300, Percent_Identity=37.3333333333333, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45551540, Length=320, Percent_Identity=35.625, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24651458, Length=338, Percent_Identity=27.810650887574, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24651456, Length=338, Percent_Identity=27.810650887574, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI281362873, Length=338, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24651454, Length=338, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI45552010, Length=359, Percent_Identity=26.4623955431755, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24651462, Length=359, Percent_Identity=26.4623955431755, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24651464, Length=359, Percent_Identity=26.4623955431755, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 32863; Mature: 32863

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIIGSASKQLGDSIASGLNAQLFPNQVSRFADGEVNVEVANDLRNQEVYIVQSLSSPV
CEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
NDNLMELLLTIDAAKRSGAKRITAIIPYYGYSRQDRVIKNNNMQSALSAKLTANLIQIAG
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCC
ANSVAAIDLHSSQIEGFFDIPVTNLSCFEVFVNSIYKENLAIVAPDVGAIGRARAFAKIL
CCCEEEEEECHHHCCEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
EEKHKLDNDIVVVDKYREKAGTSEVMNVIGEVANKNCVIVDDIVDSGGTLCNAALALKNR
HHHHCCCCCEEEEECHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCC
GAKSVVSCITHGILSGNAVEKISSSSLDKLVITDTVFHKFEKNDKIEVVSIANILICFMQ
CHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEHHHHHHHHCCCCCEEEEHHHHHHHHHHC
GGKSAS
CCCCCC
>Mature Secondary Structure
MKVIIGSASKQLGDSIASGLNAQLFPNQVSRFADGEVNVEVANDLRNQEVYIVQSLSSPV
CEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
NDNLMELLLTIDAAKRSGAKRITAIIPYYGYSRQDRVIKNNNMQSALSAKLTANLIQIAG
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCC
ANSVAAIDLHSSQIEGFFDIPVTNLSCFEVFVNSIYKENLAIVAPDVGAIGRARAFAKIL
CCCEEEEEECHHHCCEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
EEKHKLDNDIVVVDKYREKAGTSEVMNVIGEVANKNCVIVDDIVDSGGTLCNAALALKNR
HHHHCCCCCEEEEECHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCC
GAKSVVSCITHGILSGNAVEKISSSSLDKLVITDTVFHKFEKNDKIEVVSIANILICFMQ
CHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEHHHHHHHHCCCCCEEEEHHHHHHHHHHC
GGKSAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]