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The map label for this gene is ispG

Identifier: 190570649

GI number: 190570649

Start: 203187

End: 204467

Strand: Reverse

Name: ispG

Synonym: WPa_0196

Alternate gene names: 190570649

Gene position: 204467-203187 (Counterclockwise)

Preceding gene: 190570650

Following gene: 190570648

Centisome position: 13.79

GC content: 37.55

Gene sequence:

>1281_bases
ATGCTAGATAGAGACCTGACATTGAGTGATGATGCATATGAGTCATCGCCGGTTTCCAGGCATAAAACTCATACTGTAAA
AGTTGGACAAGTGAAGATAGGTGGAAATAATCCTATAGTTGTTCAATCCATGGCACTTGGTGCACATATAGATTCTGACA
ACATAAAAAGTAGTGCTCAGAAATATGCAAAAGAAGTAATAGAACTAGCGCATGCAGGTTCAGAATTGGTGCGAATTGCT
TTGAACTCAGAGGAAGTAGCAAAAGCAATACCTTATATAGTAGAGGAAATAAATAAAGAAGGCTTTGATGGTAAGATATT
AGTAGGCTGTGGACAATATGAGCTCTACAGGCTGATTCAGGATTATCCAGACAACATTAAAATTCTGGGTAAAATTAGGA
TAAATCCAGGTAATATAGGCTTTGGTGATAAACGTGATGAAAAATTCGAAAAAATTATAGAGTATGCAATAACGCATGAT
CTTCCTGTCAGAATTGGAGTAAATTGGGGTAGTCTTGATAAGTACCTTTCACAAAAATTAATGGATGAAAACTCCTTATC
TAGTAATCCAAAAACTTCTGATGTTATATTACGTAAGACGCTTGTAATGTCTGCTCTTGGTAGTGCAAAAAAAGCTGAAA
AGATTGGCCTTAATGCAGAGAAAATAATCATTTCATGTAAAGTCAGTAGAGTGCAGGATTTAATTTTAGTTTATACGGCA
CTTGCAAAATCTTCCAATTATGCGCTGCATTTGGGTTTAACCGAAGCTGGTATGGGTAACAAAGGCGTAGTAAATACCAC
AGCAGGGCTTACTTATTTATTGCAAAATGGTATTGGAGACACTATCCGGGCTTCTTTAACTCAGCGCCCTGGTGAATCAC
GCACTAATGAGGTGGTAGTATGTCAGGAAATACTGCAGTCTATAGGTTTGTGCTACTTTAACCCTCAGGTGAGTTCATGT
CCTGGTTGTGGACGCACAAGTAGCGATCGTTTTCGTATATTAACTGAGGAAGTAAATGGCTACATAAAAACTCATATGCC
GGTATGGAAGAAAAAGAATCCTGGTGTAGAGTATATGAAAGTTGCTGTTATGGGATGCATAGTAAATGGTCCTGGAGAGA
GCAAACATGCAAATCTGGGGATCAGCCTGCCTGGGTATGGAGAAAAACCTGTTTCAGCAGTCTACAAAGACGGCAAATAT
TTCAAAACTTTACAAGGTGATAATATTTTTGAAGAATTTAAGGAAATTATTAGTGATTATGTGGAGAAGCATTACACATA
A

Upstream 100 bases:

>100_bases
TCTATTACTGATCTAAATAGAACACCACAGCATAGTTTAAATGGTTCAAGTTCATTTGGCAGTGTAGTTGACACAGTAAA
ATAGTGTCACGATTAATAAT

Downstream 100 bases:

>100_bases
GCCTTTCACAAGTTTGTACTTAGACTACATTCTGTTTAGTGAACGTCAAATGAGACAATAAAGAACGTAAATTTAAAAAA
TAGATAATAGGGTTTCTTCT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLDRDLTLSDDAYESSPVSRHKTHTVKVGQVKIGGNNPIVVQSMALGAHIDSDNIKSSAQKYAKEVIELAHAGSELVRIA
LNSEEVAKAIPYIVEEINKEGFDGKILVGCGQYELYRLIQDYPDNIKILGKIRINPGNIGFGDKRDEKFEKIIEYAITHD
LPVRIGVNWGSLDKYLSQKLMDENSLSSNPKTSDVILRKTLVMSALGSAKKAEKIGLNAEKIIISCKVSRVQDLILVYTA
LAKSSNYALHLGLTEAGMGNKGVVNTTAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVVCQEILQSIGLCYFNPQVSSC
PGCGRTSSDRFRILTEEVNGYIKTHMPVWKKKNPGVEYMKVAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKY
FKTLQGDNIFEEFKEIISDYVEKHYT

Sequences:

>Translated_426_residues
MLDRDLTLSDDAYESSPVSRHKTHTVKVGQVKIGGNNPIVVQSMALGAHIDSDNIKSSAQKYAKEVIELAHAGSELVRIA
LNSEEVAKAIPYIVEEINKEGFDGKILVGCGQYELYRLIQDYPDNIKILGKIRINPGNIGFGDKRDEKFEKIIEYAITHD
LPVRIGVNWGSLDKYLSQKLMDENSLSSNPKTSDVILRKTLVMSALGSAKKAEKIGLNAEKIIISCKVSRVQDLILVYTA
LAKSSNYALHLGLTEAGMGNKGVVNTTAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVVCQEILQSIGLCYFNPQVSSC
PGCGRTSSDRFRILTEEVNGYIKTHMPVWKKKNPGVEYMKVAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKY
FKTLQGDNIFEEFKEIISDYVEKHYT
>Mature_426_residues
MLDRDLTLSDDAYESSPVSRHKTHTVKVGQVKIGGNNPIVVQSMALGAHIDSDNIKSSAQKYAKEVIELAHAGSELVRIA
LNSEEVAKAIPYIVEEINKEGFDGKILVGCGQYELYRLIQDYPDNIKILGKIRINPGNIGFGDKRDEKFEKIIEYAITHD
LPVRIGVNWGSLDKYLSQKLMDENSLSSNPKTSDVILRKTLVMSALGSAKKAEKIGLNAEKIIISCKVSRVQDLILVYTA
LAKSSNYALHLGLTEAGMGNKGVVNTTAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVVCQEILQSIGLCYFNPQVSSC
PGCGRTSSDRFRILTEEVNGYIKTHMPVWKKKNPGVEYMKVAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKY
FKTLQGDNIFEEFKEIISDYVEKHYT

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=407, Percent_Identity=28.5012285012285, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_WOLPP (B3CNN4)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975007.1
- EnsemblBacteria:   EBWOLT00000001094
- GeneID:   6384352
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0196
- GeneTree:   EBGT00050000031450
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 46868; Mature: 46868

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRDLTLSDDAYESSPVSRHKTHTVKVGQVKIGGNNPIVVQSMALGAHIDSDNIKSSAQ
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHH
KYAKEVIELAHAGSELVRIALNSEEVAKAIPYIVEEINKEGFDGKILVGCGQYELYRLIQ
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHH
DYPDNIKILGKIRINPGNIGFGDKRDEKFEKIIEYAITHDLPVRIGVNWGSLDKYLSQKL
CCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
MDENSLSSNPKTSDVILRKTLVMSALGSAKKAEKIGLNAEKIIISCKVSRVQDLILVYTA
HCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCHHEEEEEEEHHHHHHHHHHHHH
LAKSSNYALHLGLTEAGMGNKGVVNTTAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVV
HHCCCCCEEEEEEHCCCCCCCCEEECHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHH
CQEILQSIGLCYFNPQVSSCPGCGRTSSDRFRILTEEVNGYIKTHMPVWKKKNPGVEYMK
HHHHHHHCCEEEECCCCCCCCCCCCCCCCCEEEEEHHHCCEEEECCCCCCCCCCCHHHHH
VAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKYFKTLQGDNIFEEFKEIISDY
HHHHHEEECCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
VEKHYT
HHHCCC
>Mature Secondary Structure
MLDRDLTLSDDAYESSPVSRHKTHTVKVGQVKIGGNNPIVVQSMALGAHIDSDNIKSSAQ
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHH
KYAKEVIELAHAGSELVRIALNSEEVAKAIPYIVEEINKEGFDGKILVGCGQYELYRLIQ
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHH
DYPDNIKILGKIRINPGNIGFGDKRDEKFEKIIEYAITHDLPVRIGVNWGSLDKYLSQKL
CCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
MDENSLSSNPKTSDVILRKTLVMSALGSAKKAEKIGLNAEKIIISCKVSRVQDLILVYTA
HCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCHHEEEEEEEHHHHHHHHHHHHH
LAKSSNYALHLGLTEAGMGNKGVVNTTAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVV
HHCCCCCEEEEEEHCCCCCCCCEEECHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHH
CQEILQSIGLCYFNPQVSSCPGCGRTSSDRFRILTEEVNGYIKTHMPVWKKKNPGVEYMK
HHHHHHHCCEEEECCCCCCCCCCCCCCCCCEEEEEHHHCCEEEECCCCCCCCCCCHHHHH
VAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKYFKTLQGDNIFEEFKEIISDY
HHHHHEEECCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
VEKHYT
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA