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The map label for this gene is rfaF [H]

Identifier: 189499146

GI number: 189499146

Start: 158308

End: 159345

Strand: Direct

Name: rfaF [H]

Synonym: Cphamn1_0160

Alternate gene names: 189499146

Gene position: 158308-159345 (Clockwise)

Preceding gene: 189499145

Following gene: 189499147

Centisome position: 5.79

GC content: 49.13

Gene sequence:

>1038_bases
ATGGCTTTGCGTCCGGAAAAAAATCAGGAGATCGGGAAAATCCTGGTAATCAGGTTGAGCTCCATTGGAGATATTGTGTT
GACGACTCCTGTCCTTCGCCGTTTACGCGCCGCATACCCTGATGCGGTTATCGACTACTGTATCAAGCCGCCTTTTGCTT
CCCTTTTGAACGCTTCCCCTTATCTGAACACCGTCTACACCACTGCTTCTCCTCCGGATGGGCATTACGATATGGTTGTC
GATCTGCAGAACAACCTGCGTTCTCGCAAAATTTTGAAGAACATTTCCTGTGATGCGGTGTGTCGTTATCACAAAATGAA
CTGGAAAAAACTGCTGCTTGTCCGAACAGGCCTCAATCTCTTCAGAGCACCCGATTCTGTTGTCGAGAGATATATGGCTT
CCATACCATGGAACGGTAAAGGGTGCGACGGTATGGGCTGTGAGCTTTGGCTTTCCGAAGATGACCGCGGCTTTGCAGCA
TCTTTCAATGCCGGAGACGAGTACCGGCTCGCGGTTTGTTTTGGGGCTAACCATCTGACCAAGCGTTATCCCCCACTCAG
ATTTGCCTCGATTATTGAGTTGTTGGGCCGGAGACACCGGCTGCAATTTTTTCTGCTCGGAGGAACTGAAGATGTTCGCC
ATGCAGAGGAAATCATTGCTGCTCTTTCTTCCGGTACGGCATCAAGCGTTGTGAATCTCGCCGGAAAGACATCTCTAACC
CGTTCGGCGGCGGTTCTGGAGAGCTGTGATGCAGTGTTGAGCAACGACACTGGGCTAATGCATGTCGCGTCCTCTTTCGG
AAAACAGCTGTTCGTGCTTTTCGGCTCTTCGGTGGCTGAGTTCGGTTTTCTTCCTTACAACGCCCCCTATTCACTTTTCG
AAGTCCGTAATTTATCCTGCAGACCCTGTTCGCATATCGGCAGAGATCGTTGTCCGAAAGGACATTTCAGGTGTATGATG
GATATATCGGAAACGTCGGTATCGGAAAAGATTTCGGATTATTTCAAAAAGGAAAACCTGAAACGAGACAGTTTATGA

Upstream 100 bases:

>100_bases
GAGGCTTCAGAAAATGATCGCCGACAATATCGCCATGCTGCAAACTTCAATGCCTGAGGATACTGCAAAACGCAAGGCAT
GGTGGGAATTCTGGTCCTGA

Downstream 100 bases:

>100_bases
GGATAGCAAGCTGGAACATCAACGGCATCCGTGCGCGCAGGGACAGTTTGCTCGGATGGATCGACCGGAAAAAACCGGAT
ATTTTGGTGCTCGAAGAACT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MALRPEKNQEIGKILVIRLSSIGDIVLTTPVLRRLRAAYPDAVIDYCIKPPFASLLNASPYLNTVYTTASPPDGHYDMVV
DLQNNLRSRKILKNISCDAVCRYHKMNWKKLLLVRTGLNLFRAPDSVVERYMASIPWNGKGCDGMGCELWLSEDDRGFAA
SFNAGDEYRLAVCFGANHLTKRYPPLRFASIIELLGRRHRLQFFLLGGTEDVRHAEEIIAALSSGTASSVVNLAGKTSLT
RSAAVLESCDAVLSNDTGLMHVASSFGKQLFVLFGSSVAEFGFLPYNAPYSLFEVRNLSCRPCSHIGRDRCPKGHFRCMM
DISETSVSEKISDYFKKENLKRDSL

Sequences:

>Translated_345_residues
MALRPEKNQEIGKILVIRLSSIGDIVLTTPVLRRLRAAYPDAVIDYCIKPPFASLLNASPYLNTVYTTASPPDGHYDMVV
DLQNNLRSRKILKNISCDAVCRYHKMNWKKLLLVRTGLNLFRAPDSVVERYMASIPWNGKGCDGMGCELWLSEDDRGFAA
SFNAGDEYRLAVCFGANHLTKRYPPLRFASIIELLGRRHRLQFFLLGGTEDVRHAEEIIAALSSGTASSVVNLAGKTSLT
RSAAVLESCDAVLSNDTGLMHVASSFGKQLFVLFGSSVAEFGFLPYNAPYSLFEVRNLSCRPCSHIGRDRCPKGHFRCMM
DISETSVSEKISDYFKKENLKRDSL
>Mature_344_residues
ALRPEKNQEIGKILVIRLSSIGDIVLTTPVLRRLRAAYPDAVIDYCIKPPFASLLNASPYLNTVYTTASPPDGHYDMVVD
LQNNLRSRKILKNISCDAVCRYHKMNWKKLLLVRTGLNLFRAPDSVVERYMASIPWNGKGCDGMGCELWLSEDDRGFAAS
FNAGDEYRLAVCFGANHLTKRYPPLRFASIIELLGRRHRLQFFLLGGTEDVRHAEEIIAALSSGTASSVVNLAGKTSLTR
SAAVLESCDAVLSNDTGLMHVASSFGKQLFVLFGSSVAEFGFLPYNAPYSLFEVRNLSCRPCSHIGRDRCPKGHFRCMMD
ISETSVSEKISDYFKKENLKRDSL

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=358, Percent_Identity=27.0949720670391, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 38462; Mature: 38331

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRPEKNQEIGKILVIRLSSIGDIVLTTPVLRRLRAAYPDAVIDYCIKPPFASLLNASP
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCC
YLNTVYTTASPPDGHYDMVVDLQNNLRSRKILKNISCDAVCRYHKMNWKKLLLVRTGLNL
CCCEEEECCCCCCCCEEEEEEEHHCHHHHHHHHHCCHHHHHHHHHCCHHEEEEEHHCCHH
FRAPDSVVERYMASIPWNGKGCDGMGCELWLSEDDRGFAASFNAGDEYRLAVCFGANHLT
HCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCEEEEEEECCCHHH
KRYPPLRFASIIELLGRRHRLQFFLLGGTEDVRHAEEIIAALSSGTASSVVNLAGKTSLT
HCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
RSAAVLESCDAVLSNDTGLMHVASSFGKQLFVLFGSSVAEFGFLPYNAPYSLFEVRNLSC
HHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEHHCCHHHHHCCCCCCCCCHHHHHCCCCC
RPCSHIGRDRCPKGHFRCMMDISETSVSEKISDYFKKENLKRDSL
CCHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALRPEKNQEIGKILVIRLSSIGDIVLTTPVLRRLRAAYPDAVIDYCIKPPFASLLNASP
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCC
YLNTVYTTASPPDGHYDMVVDLQNNLRSRKILKNISCDAVCRYHKMNWKKLLLVRTGLNL
CCCEEEECCCCCCCCEEEEEEEHHCHHHHHHHHHCCHHHHHHHHHCCHHEEEEEHHCCHH
FRAPDSVVERYMASIPWNGKGCDGMGCELWLSEDDRGFAASFNAGDEYRLAVCFGANHLT
HCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCEEEEEEECCCHHH
KRYPPLRFASIIELLGRRHRLQFFLLGGTEDVRHAEEIIAALSSGTASSVVNLAGKTSLT
HCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
RSAAVLESCDAVLSNDTGLMHVASSFGKQLFVLFGSSVAEFGFLPYNAPYSLFEVRNLSC
HHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEHHCCHHHHHCCCCCCCCCHHHHHCCCCC
RPCSHIGRDRCPKGHFRCMMDISETSVSEKISDYFKKENLKRDSL
CCHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]