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The map label for this gene is exoA [H]

Identifier: 189499147

GI number: 189499147

Start: 159342

End: 160112

Strand: Direct

Name: exoA [H]

Synonym: Cphamn1_0161

Alternate gene names: 189499147

Gene position: 159342-160112 (Clockwise)

Preceding gene: 189499146

Following gene: 189499148

Centisome position: 5.82

GC content: 50.45

Gene sequence:

>771_bases
ATGAGGATAGCAAGCTGGAACATCAACGGCATCCGTGCGCGCAGGGACAGTTTGCTCGGATGGATCGACCGGAAAAAACC
GGATATTTTGGTGCTCGAAGAACTCAAGGCACAGGAGAAGGATATTCCCGAAGAGATAGTGGCGATGCAGGGATATAAAA
AATACTGGAACGGCTCGGCATTCAGGAAGGGTTACAGCGGGGTCGGCCTTCTTCTTAAAGAAGGGCTTTGTGGCGAGTGC
CGCCTTGAAATACCGGAGTTTGACTGCGAAAACCGGACAGTCGTCCTTCATGGCAGTAACCTCAGTGTTATCGGAACCTA
TGTCCCGAGAGGCGATGGAGATGCACACTACGCGGTAAAACTCGGTTATCTTTCAGACCTGGGCACCTATATTCTTGAAC
TTCTGCGGGAGGGAAGGCAGGTGATACATACCGGAGATATCAATGTGGCCCACACAGATCTCGATGTACATCACTCCCAG
AACAAGCCCGGTATAGTCGGGCTTCGTCCTGAAGAAAGGGCGGCTATTGACAGGCATCTTGAGATTGGCCTGCACGATAT
TATGAGAGAGCGGTTTCCTGACAAAAAAGACATGTTCACCTGGTGGCCATACTGGAAGGGTGCCCGTGAACGAAATCTTG
GATGGAGAATTGATTGCTTTTATCTGTCGAAAGGGCTTATCGGGGATGTGGTTGACGTTCGCATTGATAGTGCTGAAAAA
AGCTCGGACCATGCTCCGGTAATTCTGGATATCCGCTCTTGCGCTCTCTGA

Upstream 100 bases:

>100_bases
CGAAAGGACATTTCAGGTGTATGATGGATATATCGGAAACGTCGGTATCGGAAAAGATTTCGGATTATTTCAAAAAGGAA
AACCTGAAACGAGACAGTTT

Downstream 100 bases:

>100_bases
GAACTCGCGGTGAGTACTGTTTTTCCGGACGCGACGAGGGTGTTGCGGCTGGATTTATAGCTGTTGGAAATCTTGTTGAA
ATCCCTTACCTTTAAGGTTC

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRIASWNINGIRARRDSLLGWIDRKKPDILVLEELKAQEKDIPEEIVAMQGYKKYWNGSAFRKGYSGVGLLLKEGLCGEC
RLEIPEFDCENRTVVLHGSNLSVIGTYVPRGDGDAHYAVKLGYLSDLGTYILELLREGRQVIHTGDINVAHTDLDVHHSQ
NKPGIVGLRPEERAAIDRHLEIGLHDIMRERFPDKKDMFTWWPYWKGARERNLGWRIDCFYLSKGLIGDVVDVRIDSAEK
SSDHAPVILDIRSCAL

Sequences:

>Translated_256_residues
MRIASWNINGIRARRDSLLGWIDRKKPDILVLEELKAQEKDIPEEIVAMQGYKKYWNGSAFRKGYSGVGLLLKEGLCGEC
RLEIPEFDCENRTVVLHGSNLSVIGTYVPRGDGDAHYAVKLGYLSDLGTYILELLREGRQVIHTGDINVAHTDLDVHHSQ
NKPGIVGLRPEERAAIDRHLEIGLHDIMRERFPDKKDMFTWWPYWKGARERNLGWRIDCFYLSKGLIGDVVDVRIDSAEK
SSDHAPVILDIRSCAL
>Mature_256_residues
MRIASWNINGIRARRDSLLGWIDRKKPDILVLEELKAQEKDIPEEIVAMQGYKKYWNGSAFRKGYSGVGLLLKEGLCGEC
RLEIPEFDCENRTVVLHGSNLSVIGTYVPRGDGDAHYAVKLGYLSDLGTYILELLREGRQVIHTGDINVAHTDLDVHHSQ
NKPGIVGLRPEERAAIDRHLEIGLHDIMRERFPDKKDMFTWWPYWKGARERNLGWRIDCFYLSKGLIGDVVDVRIDSAEK
SSDHAPVILDIRSCAL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=261, Percent_Identity=31.8007662835249, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI18375503, Length=261, Percent_Identity=31.8007662835249, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI18375501, Length=261, Percent_Identity=31.8007662835249, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1788046, Length=271, Percent_Identity=32.4723247232472, Blast_Score=128, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=31.5175097276265, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI221330655, Length=259, Percent_Identity=31.2741312741313, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=31.2741312741313, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29165; Mature: 29165

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIASWNINGIRARRDSLLGWIDRKKPDILVLEELKAQEKDIPEEIVAMQGYKKYWNGSA
CEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCH
FRKGYSGVGLLLKEGLCGECRLEIPEFDCENRTVVLHGSNLSVIGTYVPRGDGDAHYAVK
HHCCCCCHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEE
LGYLSDLGTYILELLREGRQVIHTGDINVAHTDLDVHHSQNKPGIVGLRPEERAAIDRHL
ECCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCEEEECCHHHHHHHHHH
EIGLHDIMRERFPDKKDMFTWWPYWKGARERNLGWRIDCFYLSKGLIGDVVDVRIDSAEK
HHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCC
SSDHAPVILDIRSCAL
CCCCCCEEEEECCCCC
>Mature Secondary Structure
MRIASWNINGIRARRDSLLGWIDRKKPDILVLEELKAQEKDIPEEIVAMQGYKKYWNGSA
CEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCH
FRKGYSGVGLLLKEGLCGECRLEIPEFDCENRTVVLHGSNLSVIGTYVPRGDGDAHYAVK
HHCCCCCHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEE
LGYLSDLGTYILELLREGRQVIHTGDINVAHTDLDVHHSQNKPGIVGLRPEERAAIDRHL
ECCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCEEEECCHHHHHHHHHH
EIGLHDIMRERFPDKKDMFTWWPYWKGARERNLGWRIDCFYLSKGLIGDVVDVRIDSAEK
HHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCC
SSDHAPVILDIRSCAL
CCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]