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The map label for this gene is putA [C]

Identifier: 189499130

GI number: 189499130

Start: 135289

End: 135645

Strand: Direct

Name: putA [C]

Synonym: Cphamn1_0140

Alternate gene names: 189499130

Gene position: 135289-135645 (Clockwise)

Preceding gene: 189499128

Following gene: 189499131

Centisome position: 4.94

GC content: 46.22

Gene sequence:

>357_bases
ATGCAGTTTGTATTTGGACTCATGGATTTTGATTTGGTTGCTAAAGGTTGCGATAAGTGTTACTTTATGTTAAAAGGAGT
TAAGATCATGTCAAAGACAAAAGGCGTCAAGTTAGACGACACTACCCAGCAACGGCTTGCAGCACTGGCTCGTATTCGTG
ACAGGTCGCCTCACTGGCTCATGTGCAAGGCTATCGAAACATTTCTGGATCGTGAAGAAAGATACGAGCAGGAAAAACGT
GAGGATATGGAGCGTTGGGAACGTTATCAATTGACCGGCGATGCAGTGCCCCATGAAAAGGCTGCCGAGTGGATCGAAAA
TCTGGCGCAAGGAAAGGTGACGGCTTGTCCCAAATAA

Upstream 100 bases:

>100_bases
ACTATGTTGACCCATGGTCATGGATGCCTGACGGGAAGTAAAAATCACCGTGGTAAGTATCCATTCTTTCTGCTATCCAG
CGGTCCCAAATCCTGATCGG

Downstream 100 bases:

>100_bases
AATGGCTTCTGGAAGCGTTGGCCGATGTCGAGCGGCTTCACGCTTTTCTGCACGAAAAGAGCCCCGATGCGGCTGCACGA
GCAGCAAAGGTCATTCTGGA

Product: hypothetical protein

Products: 1-Pyrroline-5-carboxylate; FADH2; H+ [C]

Alternate protein names: None

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MQFVFGLMDFDLVAKGCDKCYFMLKGVKIMSKTKGVKLDDTTQQRLAALARIRDRSPHWLMCKAIETFLDREERYEQEKR
EDMERWERYQLTGDAVPHEKAAEWIENLAQGKVTACPK

Sequences:

>Translated_118_residues
MQFVFGLMDFDLVAKGCDKCYFMLKGVKIMSKTKGVKLDDTTQQRLAALARIRDRSPHWLMCKAIETFLDREERYEQEKR
EDMERWERYQLTGDAVPHEKAAEWIENLAQGKVTACPK
>Mature_118_residues
MQFVFGLMDFDLVAKGCDKCYFMLKGVKIMSKTKGVKLDDTTQQRLAALARIRDRSPHWLMCKAIETFLDREERYEQEKR
EDMERWERYQLTGDAVPHEKAAEWIENLAQGKVTACPK

Specific function: Oxidizes Proline To Glutamate For Use As A Carbon And Nitrogen Source And Also Function As A Transcriptional Repressor Of The Put Operon. [C]

COG id: COG3905

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.99.8; 1.5.1.12 [C]

Molecular weight: Translated: 13863; Mature: 13863

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFVFGLMDFDLVAKGCDKCYFMLKGVKIMSKTKGVKLDDTTQQRLAALARIRDRSPHWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCH
MCKAIETFLDREERYEQEKREDMERWERYQLTGDAVPHEKAAEWIENLAQGKVTACPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQFVFGLMDFDLVAKGCDKCYFMLKGVKIMSKTKGVKLDDTTQQRLAALARIRDRSPHWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCH
MCKAIETFLDREERYEQEKREDMERWERYQLTGDAVPHEKAAEWIENLAQGKVTACPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 45 {@PROL01.txt*L-proline!}} [C]

Substrates: FAD; L-Proline [C]

Specific reaction: FAD + L-Proline --> 1-Pyrroline-5-carboxylate + FADH2 + H+ [C]

General reaction: Redox reaction [C]

Inhibitor: L-Azetidine-2-carboxylate; Lactate; Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA