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The map label for this gene is rasP [H]

Identifier: 189499005

GI number: 189499005

Start: 4548

End: 5300

Strand: Direct

Name: rasP [H]

Synonym: Cphamn1_0005

Alternate gene names: 189499005

Gene position: 4548-5300 (Clockwise)

Preceding gene: 189499004

Following gene: 189499009

Centisome position: 0.17

GC content: 46.61

Gene sequence:

>753_bases
ATGGTTACGGCAATATTTGCCTTTGTTTGTATATTTTCACTCGTTGTGCTTGTGCATGAGTTAGGTCACTTTCTTGCTGC
GCGAAGAGTTGGTGTTCCGGTATATGAGTTTTCCATAGGGTTTCCGTTCAGCCCAAGGATTGTAACGTTATTCAGGCACC
GTGAAACTGAATTCACGTTACGTCTCCTTCCTCTTGGCGGTTTTGTAAGCTTTTCGAATGAAGATGATGAAGAAGCGGAG
AAACTCTTTGGCGCTTCACGTGTTTCACGCGCGTTAGTCATGTCGGGTGGTTCTCTTTTCAATCTTGTGTTTGCTTTTTT
GCTGTTTGTTCCTGCGTTTATGGCAGGCGAAGGAGTGCCGCTGTTGAGTGCAGCAAGCGTCAGTATGCAGACGATAGGCA
CCGTTGCTTCTGAAACCATTCATCTTCTTTTCACTCTGTTCACAGGCAGTGCCGGTATGGAAAATCTGTCAGGACCGGTA
GGTATTGCCGTACTTGCCGGACAGGCTGCAAGCGGGGGATGGGTAAATCTTCTGTTTTTTACCGGTTTTCTCAGTTTGAG
CCTCGGTATCATGAATATGTTGCCTTTTCCAGGGCTGGATGGCGGACAACTTGCCATGCTTATGATTGAAACCGTCAGGA
ACAAACCCATCGGAGCAAGAGCTCATCAGTTCATCAATCTCGCGGGAATCATGCTGTTTATTGTACTCTCGTTGTTTGTA
ACATGGCAGGACATCTCGAGAATCATTGGTTGA

Upstream 100 bases:

>100_bases
TATTATTGGTGTGTTGCAGGATTTGAATAATAACGATCAGTCAACGAAGCGCATTGAGAGTGGTAGTAGTTTAGTAATAA
TCTAAGATTAAATAATCATT

Downstream 100 bases:

>100_bases
ACATAGATCTACTCTCAATACATAAGGGGCTGTCTCAGAAGCCTGTCACCTCTGGTTCAAACCACAGAAAAAAGTTGGTT
TTTGTTGAACTGTCGAGGAA

Product: peptidase M50

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MVTAIFAFVCIFSLVVLVHELGHFLAARRVGVPVYEFSIGFPFSPRIVTLFRHRETEFTLRLLPLGGFVSFSNEDDEEAE
KLFGASRVSRALVMSGGSLFNLVFAFLLFVPAFMAGEGVPLLSAASVSMQTIGTVASETIHLLFTLFTGSAGMENLSGPV
GIAVLAGQAASGGWVNLLFFTGFLSLSLGIMNMLPFPGLDGGQLAMLMIETVRNKPIGARAHQFINLAGIMLFIVLSLFV
TWQDISRIIG

Sequences:

>Translated_250_residues
MVTAIFAFVCIFSLVVLVHELGHFLAARRVGVPVYEFSIGFPFSPRIVTLFRHRETEFTLRLLPLGGFVSFSNEDDEEAE
KLFGASRVSRALVMSGGSLFNLVFAFLLFVPAFMAGEGVPLLSAASVSMQTIGTVASETIHLLFTLFTGSAGMENLSGPV
GIAVLAGQAASGGWVNLLFFTGFLSLSLGIMNMLPFPGLDGGQLAMLMIETVRNKPIGARAHQFINLAGIMLFIVLSLFV
TWQDISRIIG
>Mature_250_residues
MVTAIFAFVCIFSLVVLVHELGHFLAARRVGVPVYEFSIGFPFSPRIVTLFRHRETEFTLRLLPLGGFVSFSNEDDEEAE
KLFGASRVSRALVMSGGSLFNLVFAFLLFVPAFMAGEGVPLLSAASVSMQTIGTVASETIHLLFTLFTGSAGMENLSGPV
GIAVLAGQAASGGWVNLLFFTGFLSLSLGIMNMLPFPGLDGGQLAMLMIETVRNKPIGARAHQFINLAGIMLFIVLSLFV
TWQDISRIIG

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor [

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=110, Percent_Identity=39.0909090909091, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 26929; Mature: 26929

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTAIFAFVCIFSLVVLVHELGHFLAARRVGVPVYEFSIGFPFSPRIVTLFRHRETEFTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCEEE
RLLPLGGFVSFSNEDDEEAEKLFGASRVSRALVMSGGSLFNLVFAFLLFVPAFMAGEGVP
EEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
LLSAASVSMQTIGTVASETIHLLFTLFTGSAGMENLSGPVGIAVLAGQAASGGWVNLLFF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
TGFLSLSLGIMNMLPFPGLDGGQLAMLMIETVRNKPIGARAHQFINLAGIMLFIVLSLFV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TWQDISRIIG
HHHHHHHHCC
>Mature Secondary Structure
MVTAIFAFVCIFSLVVLVHELGHFLAARRVGVPVYEFSIGFPFSPRIVTLFRHRETEFTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCEEE
RLLPLGGFVSFSNEDDEEAEKLFGASRVSRALVMSGGSLFNLVFAFLLFVPAFMAGEGVP
EEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
LLSAASVSMQTIGTVASETIHLLFTLFTGSAGMENLSGPVGIAVLAGQAASGGWVNLLFF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
TGFLSLSLGIMNMLPFPGLDGGQLAMLMIETVRNKPIGARAHQFINLAGIMLFIVLSLFV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TWQDISRIIG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA