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The map label for this gene is yfnG [H]

Identifier: 189352667

GI number: 189352667

Start: 757341

End: 758315

Strand: Reverse

Name: yfnG [H]

Synonym: BMULJ_03898

Alternate gene names: 189352667

Gene position: 758315-757341 (Counterclockwise)

Preceding gene: 189352668

Following gene: 189352645

Centisome position: 30.66

GC content: 70.05

Gene sequence:

>975_bases
ATGACACGTTCTGATGCCGGACGCGCGGCGCCGCGCCGCGCGTTCGTCACCGGCCTGACGGGCTTCACGGGCCGCTACAT
CGCGCAATGCCTCGAAGACGCCGGCTATGAAGTCTGGGGCACGGTCGCACCGGGCGCGCCGCATCCGGACAATCCGGCGC
TCGCGCGTTGCACGCTGCTGAGCGTCGACCTGCTCGACGCCGACGCCGTGCGCGCGGCCGCCGCCGACGCGCGGCCCGAT
GCCGTCGTGCATCTCGCTGCGCGCGCGCATGTCGCGAACGACGAGCCCTCGCAGACGTATGCCGTCAACATCATGGGCAC
GCGCAACCTGCTCGCCGCGCTGGCCGGCCTCGATCGCCGGCCGTCGGCGGTGCTGCTCGCGAGCAGCGCCAACATCTACG
GCAACGCGACGGCCGGCGTGCTCGACGAAAGCGCGCCGCCCGCGCCCGCGAACGACTACGCGGTCAGCAAGCTCGCGATG
GAATACGCGGCGAAGCTATGGACCGACCGGCTGCCGATCGTGATCGCCCGCCCGTTCAACTACACGGGCGTCGGCCAGAG
CGAAGCGTATCTGCTGCCGAAGCTCGTCGCGCACTATGCGCGCAACGCGCCGCGCATCTCGCTCGGCAATTTCGACGTGA
GCCGCGACTTCTCCGACGTGCGCGACGTGACGGCCGCGTATCTGCGGCTGCTCGAAGCCGCGCCGGCCGGCGACACGTTC
AACGTCTGCTCCGAGCGCGCGTATTCGCTGAAGGAAGTGCTGGCGATGCTGTCGCGCATCGCCGGCTACGTGATCGACGT
AACGGTCGATCCGCGTTTCGTGCGGCACAACGAAGTGAAGCACCTGAGCGGATCGCGCGACAAGCTGCGGCGCGCGATCG
GCGAACTGCCGCTCACGCCGCTCGACGAGACGCTGCGATGGATGTACGACGCGATGCGCGACGCGCAGGCCGGCGCGCCG
GCTCGCCTCGACTGA

Upstream 100 bases:

>100_bases
GCCGACAAGGCGAAAAGCAAGCTCGGCTGGGTCCCGAAGACGACGCTCGAACAACTGTGCCAGATGATGGTCGAAGCGGA
TTTGACGCGGAACACCCACC

Downstream 100 bases:

>100_bases
CGCGCTGCCGCTGCGCTGCGCGGCCGCAACCGAGCCGGCAGTAAAACGAACGCGCCGTTCGCACCGGCATAGGCCGGCGC
GAACGGCGCGTGTCGTCGAT

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTRSDAGRAAPRRAFVTGLTGFTGRYIAQCLEDAGYEVWGTVAPGAPHPDNPALARCTLLSVDLLDADAVRAAAADARPD
AVVHLAARAHVANDEPSQTYAVNIMGTRNLLAALAGLDRRPSAVLLASSANIYGNATAGVLDESAPPAPANDYAVSKLAM
EYAAKLWTDRLPIVIARPFNYTGVGQSEAYLLPKLVAHYARNAPRISLGNFDVSRDFSDVRDVTAAYLRLLEAAPAGDTF
NVCSERAYSLKEVLAMLSRIAGYVIDVTVDPRFVRHNEVKHLSGSRDKLRRAIGELPLTPLDETLRWMYDAMRDAQAGAP
ARLD

Sequences:

>Translated_324_residues
MTRSDAGRAAPRRAFVTGLTGFTGRYIAQCLEDAGYEVWGTVAPGAPHPDNPALARCTLLSVDLLDADAVRAAAADARPD
AVVHLAARAHVANDEPSQTYAVNIMGTRNLLAALAGLDRRPSAVLLASSANIYGNATAGVLDESAPPAPANDYAVSKLAM
EYAAKLWTDRLPIVIARPFNYTGVGQSEAYLLPKLVAHYARNAPRISLGNFDVSRDFSDVRDVTAAYLRLLEAAPAGDTF
NVCSERAYSLKEVLAMLSRIAGYVIDVTVDPRFVRHNEVKHLSGSRDKLRRAIGELPLTPLDETLRWMYDAMRDAQAGAP
ARLD
>Mature_323_residues
TRSDAGRAAPRRAFVTGLTGFTGRYIAQCLEDAGYEVWGTVAPGAPHPDNPALARCTLLSVDLLDADAVRAAAADARPDA
VVHLAARAHVANDEPSQTYAVNIMGTRNLLAALAGLDRRPSAVLLASSANIYGNATAGVLDESAPPAPANDYAVSKLAME
YAAKLWTDRLPIVIARPFNYTGVGQSEAYLLPKLVAHYARNAPRISLGNFDVSRDFSDVRDVTAAYLRLLEAAPAGDTFN
VCSERAYSLKEVLAMLSRIAGYVIDVTVDPRFVRHNEVKHLSGSRDKLRRAIGELPLTPLDETLRWMYDAMRDAQAGAPA
RLD

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=276, Percent_Identity=25.3623188405797, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=26.4367816091954, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI48994969, Length=250, Percent_Identity=27.6, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=336, Percent_Identity=24.702380952381, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17539422, Length=329, Percent_Identity=24.6200607902736, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=329, Percent_Identity=24.6200607902736, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=329, Percent_Identity=24.6200607902736, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=329, Percent_Identity=24.6200607902736, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=329, Percent_Identity=24.6200607902736, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24158427, Length=347, Percent_Identity=22.478386167147, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.47 [C]

Molecular weight: Translated: 34888; Mature: 34757

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSDAGRAAPRRAFVTGLTGFTGRYIAQCLEDAGYEVWGTVAPGAPHPDNPALARCTLL
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHEEEEE
SVDLLDADAVRAAAADARPDAVVHLAARAHVANDEPSQTYAVNIMGTRNLLAALAGLDRR
EEECCCHHHHHHHHCCCCCCCEEEHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHCCCCC
PSAVLLASSANIYGNATAGVLDESAPPAPANDYAVSKLAMEYAAKLWTDRLPIVIARPFN
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YTGVGQSEAYLLPKLVAHYARNAPRISLGNFDVSRDFSDVRDVTAAYLRLLEAAPAGDTF
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
NVCSERAYSLKEVLAMLSRIAGYVIDVTVDPRFVRHNEVKHLSGSRDKLRRAIGELPLTP
HHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHCCCHHHHCCCCHHHHHHHHHCCCCCC
LDETLRWMYDAMRDAQAGAPARLD
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TRSDAGRAAPRRAFVTGLTGFTGRYIAQCLEDAGYEVWGTVAPGAPHPDNPALARCTLL
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHEEEEE
SVDLLDADAVRAAAADARPDAVVHLAARAHVANDEPSQTYAVNIMGTRNLLAALAGLDRR
EEECCCHHHHHHHHCCCCCCCEEEHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHCCCCC
PSAVLLASSANIYGNATAGVLDESAPPAPANDYAVSKLAMEYAAKLWTDRLPIVIARPFN
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
YTGVGQSEAYLLPKLVAHYARNAPRISLGNFDVSRDFSDVRDVTAAYLRLLEAAPAGDTF
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
NVCSERAYSLKEVLAMLSRIAGYVIDVTVDPRFVRHNEVKHLSGSRDKLRRAIGELPLTP
HHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHCCCHHHHCCCCHHHHHHHHHCCCCCC
LDETLRWMYDAMRDAQAGAPARLD
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADP+; NADPH+; Pyridine nucleotides [C]

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.55 {GDPmannose}} 0.26 {GDPmannose}} 0.222 {GDPmannose}} [C]

Substrates: GDP-D-mannose [C]

Specific reaction: GDP-D-mannose --> GDP-4-dehydro-6-deoxy-D-mannose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: GDP-D- glucose; GDP-L-fucose; p-Chloromercuriphenyl sulfonate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]