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The map label for this gene is gmd [H]

Identifier: 189352668

GI number: 189352668

Start: 758302

End: 759345

Strand: Reverse

Name: gmd [H]

Synonym: BMULJ_03899

Alternate gene names: 189352668

Gene position: 759345-758302 (Counterclockwise)

Preceding gene: 189352669

Following gene: 189352667

Centisome position: 30.7

GC content: 64.27

Gene sequence:

>1044_bases
ATGAGCCACACTCGCAAGGCCATCATCACGGGGATCACGGGGCAGGACGGCGCCTACCTGACCAAGCTGCTGCTCGACAA
GGGCTATCAGGTCACCGGCACCTACCGCCGCACCAGCTCGGTGAACTTCTGGCGGATCGCCGAGCTCGGCGTCGACACGC
ATCCGAACCTGACGCTCGTCGAACACGATCTGACCGACGCCGGTTCGAGCCTGCGGCTGCTCGAGCGTACGCAGCCCGAC
GAGCTGTACAACCTGGCCGCGCAAAGCTTCGTCGGCGTGTCGTTCGACCAGCCGTCGACGACGGCCGAAGTCACGGGCCT
CGGCACGCTGAACCTGCTCGAGGCGATTCGCGTCGTGAGCCCGCGCACGCGCTTCTATCAGGCATCGACGTCGGAAATGT
TCGGCAAGGTGCAGTCGATTCCGCAAACGGAAACGACGGCGTTCTATCCGCGCAGCCCGTACGGCGTCGCGAAGCTGTTC
GCGCACTGGACGACCGTCAACTATCGCGAGTCGTACGGGCTGTTCGGCTGCAGCGGCATCCTGTTCAATCACGAGTCGCC
GCTGCGCGGCCGCGAGTTCGTCACGCGCAAGATCACCGATACGGTCGCGAAGATCCGCCTCGGCAAGGCGAAGCGGCTCG
AGCTCGGCAATCTCGATGCGAAGCGCGACTGGGGCTTCGCGCTCGAATACGTCGAAGGCATGTGGCGGATGCTGCAGGCC
GACGAGCCGGACACCTACGTGCTCGCGACGAACCGCACCGAAACCGTGCGCTACTTCGTGCGGATGGCGTTCGCCGCTGC
GGGCTACCAGATCGAATGGACCGGCAAGGGCGAACAGGAGCGCGGACTCGACGCGGCAACCGGCGACGTGCTCGTCGAAG
TGAATCCGAAGTTCTATCGGCCGGCCGAAGTCGATCTGCTGATCGGCTGCGCCGACAAGGCGAAAAGCAAGCTCGGCTGG
GTCCCGAAGACGACGCTCGAACAACTGTGCCAGATGATGGTCGAAGCGGATTTGACGCGGAACACCCACCATGACACGTT
CTGA

Upstream 100 bases:

>100_bases
AGCGGCGCCTGACGCGCGGCCCCGCACGCCGCACGCTGCCTGCCGCGGCACGCACGACCGCCTGATTTTCATCTTCCATC
GTTTCTACCAGGACATCGCC

Downstream 100 bases:

>100_bases
TGCCGGACGCGCGGCGCCGCGCCGCGCGTTCGTCACCGGCCTGACGGGCTTCACGGGCCGCTACATCGCGCAATGCCTCG
AAGACGCCGGCTATGAAGTC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSHTRKAIITGITGQDGAYLTKLLLDKGYQVTGTYRRTSSVNFWRIAELGVDTHPNLTLVEHDLTDAGSSLRLLERTQPD
ELYNLAAQSFVGVSFDQPSTTAEVTGLGTLNLLEAIRVVSPRTRFYQASTSEMFGKVQSIPQTETTAFYPRSPYGVAKLF
AHWTTVNYRESYGLFGCSGILFNHESPLRGREFVTRKITDTVAKIRLGKAKRLELGNLDAKRDWGFALEYVEGMWRMLQA
DEPDTYVLATNRTETVRYFVRMAFAAAGYQIEWTGKGEQERGLDAATGDVLVEVNPKFYRPAEVDLLIGCADKAKSKLGW
VPKTTLEQLCQMMVEADLTRNTHHDTF

Sequences:

>Translated_347_residues
MSHTRKAIITGITGQDGAYLTKLLLDKGYQVTGTYRRTSSVNFWRIAELGVDTHPNLTLVEHDLTDAGSSLRLLERTQPD
ELYNLAAQSFVGVSFDQPSTTAEVTGLGTLNLLEAIRVVSPRTRFYQASTSEMFGKVQSIPQTETTAFYPRSPYGVAKLF
AHWTTVNYRESYGLFGCSGILFNHESPLRGREFVTRKITDTVAKIRLGKAKRLELGNLDAKRDWGFALEYVEGMWRMLQA
DEPDTYVLATNRTETVRYFVRMAFAAAGYQIEWTGKGEQERGLDAATGDVLVEVNPKFYRPAEVDLLIGCADKAKSKLGW
VPKTTLEQLCQMMVEADLTRNTHHDTF
>Mature_346_residues
SHTRKAIITGITGQDGAYLTKLLLDKGYQVTGTYRRTSSVNFWRIAELGVDTHPNLTLVEHDLTDAGSSLRLLERTQPDE
LYNLAAQSFVGVSFDQPSTTAEVTGLGTLNLLEAIRVVSPRTRFYQASTSEMFGKVQSIPQTETTAFYPRSPYGVAKLFA
HWTTVNYRESYGLFGCSGILFNHESPLRGREFVTRKITDTVAKIRLGKAKRLELGNLDAKRDWGFALEYVEGMWRMLQAD
EPDTYVLATNRTETVRYFVRMAFAAAGYQIEWTGKGEQERGLDAATGDVLVEVNPKFYRPAEVDLLIGCADKAKSKLGWV
PKTTLEQLCQMMVEADLTRNTHHDTF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=338, Percent_Identity=53.5502958579882, Blast_Score=360, Evalue=2e-99,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=50.561797752809, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=26.3456090651558, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=309, Percent_Identity=27.831715210356, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=53.3724340175953, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=53.3724340175953, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI133901786, Length=339, Percent_Identity=53.6873156342183, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17539422, Length=339, Percent_Identity=53.6873156342183, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI133901788, Length=339, Percent_Identity=53.6873156342183, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17507723, Length=339, Percent_Identity=54.2772861356932, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=51.6034985422741, Blast_Score=356, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38977; Mature: 38845

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTRKAIITGITGQDGAYLTKLLLDKGYQVTGTYRRTSSVNFWRIAELGVDTHPNLTLV
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCCCCCEEEE
EHDLTDAGSSLRLLERTQPDELYNLAAQSFVGVSFDQPSTTAEVTGLGTLNLLEAIRVVS
ECCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCC
PRTRFYQASTSEMFGKVQSIPQTETTAFYPRSPYGVAKLFAHWTTVNYRESYGLFGCSGI
CCHHEEECCHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHEEEEHHCCCCEEEEEE
LFNHESPLRGREFVTRKITDTVAKIRLGKAKRLELGNLDAKRDWGFALEYVEGMWRMLQA
EECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHC
DEPDTYVLATNRTETVRYFVRMAFAAAGYQIEWTGKGEQERGLDAATGDVLVEVNPKFYR
CCCCEEEEEECCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCCCCCCCEEEEECCCEEC
PAEVDLLIGCADKAKSKLGWVPKTTLEQLCQMMVEADLTRNTHHDTF
CCCEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SHTRKAIITGITGQDGAYLTKLLLDKGYQVTGTYRRTSSVNFWRIAELGVDTHPNLTLV
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCCCCCEEEE
EHDLTDAGSSLRLLERTQPDELYNLAAQSFVGVSFDQPSTTAEVTGLGTLNLLEAIRVVS
ECCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCC
PRTRFYQASTSEMFGKVQSIPQTETTAFYPRSPYGVAKLFAHWTTVNYRESYGLFGCSGI
CCHHEEECCHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHEEEEHHCCCCEEEEEE
LFNHESPLRGREFVTRKITDTVAKIRLGKAKRLELGNLDAKRDWGFALEYVEGMWRMLQA
EECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHC
DEPDTYVLATNRTETVRYFVRMAFAAAGYQIEWTGKGEQERGLDAATGDVLVEVNPKFYR
CCCCEEEEEECCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCCCCCCCEEEEECCCEEC
PAEVDLLIGCADKAKSKLGWVPKTTLEQLCQMMVEADLTRNTHHDTF
CCCEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]