Click here to switch to the map view.

The map label for this gene is argT [H]

Identifier: 189352290

GI number: 189352290

Start: 338962

End: 339735

Strand: Direct

Name: argT [H]

Synonym: BMULJ_03509

Alternate gene names: 189352290

Gene position: 338962-339735 (Clockwise)

Preceding gene: 189352288

Following gene: 189352291

Centisome position: 13.71

GC content: 64.73

Gene sequence:

>774_bases
ATGAAAAAACTCGTGCTCAGTGTCGCTCTCGCGTGCATCGCCGTCGGCGCGCATGCGAAGGATTGGTCGACGATCCGCTT
CGGCGTCGACGCCAGCTATCCGCCGTTCGAATCGAAGGACGCGAGCGGCAAGGTCGTCGGCTTCGACGTCGATCTCGGCA
ACGAAATCTGCGCGCGGCTGAAAGCGAAATGCGTGTGGGTCGAAAACGACTTCGACGGGATGATCCCCGCGCTGAAGGCG
AAGAAGTTCGACGGCGTGCTGTCGTCGATGTCGATGACGCCCGCGCGCGCCGAGCAGATCGCGTTCTCCGACAAGCTGTT
CAACACGCCGACGCGCCTCGTCGCGAAGAAGGGTGCCGGGCTGCAGCCGAGCGCCGAGTCGCTGAAGGGCAAGTCGGTCG
GCGTCGAGCAGGGGACGATCCAGGAGACGTACGCGAAGGCGTACTGGGCGCCGAAGGGCGTGCAGGTCGTCCCGTATCAG
AACCAGGACGGCGTCTATCAGGACTTGATGTCGGGCCGCCTCGACGCGGCGCTGCAGGACGCCGTGCAGGCCGATCTCGG
TTTCCTGAAGACACCGCGCGGCGCGAACTTCGCGTTCGCGGGCGGCAACCTCGACGATCCGAAGACGCTCGGCAACGGCG
CGGGCATCGGGCTGCGCAAGGAAGACGCCGACCTGAAAGCGAAGATCGACGCGGCGATCGCCGCGATGCTGAAGGACGGC
ACGTACAGGAAGCTCGAGAAGAAGTATTTCGCGTTCGACGTCTACGGCGGCTGA

Upstream 100 bases:

>100_bases
CAAAGTTGTATGTACAACTTCGGCGCCGCGACGATCGCGCGCCGCGGGTTGGGGCAGATGCCGCGCGCATCTGCGGGGCT
CGGCCACTTACAGGAACGTC

Downstream 100 bases:

>100_bases
CGCCGCGGCGGCGCGCGCGGCACACGGCCTCGCGCGCCGCCTGTCTTTCCGGATGGAACCGACATGATTTTCCAGGGATT
TGGCCCGCTGCTGTGGGCCG

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKLVLSVALACIAVGAHAKDWSTIRFGVDASYPPFESKDASGKVVGFDVDLGNEICARLKAKCVWVENDFDGMIPALKA
KKFDGVLSSMSMTPARAEQIAFSDKLFNTPTRLVAKKGAGLQPSAESLKGKSVGVEQGTIQETYAKAYWAPKGVQVVPYQ
NQDGVYQDLMSGRLDAALQDAVQADLGFLKTPRGANFAFAGGNLDDPKTLGNGAGIGLRKEDADLKAKIDAAIAAMLKDG
TYRKLEKKYFAFDVYGG

Sequences:

>Translated_257_residues
MKKLVLSVALACIAVGAHAKDWSTIRFGVDASYPPFESKDASGKVVGFDVDLGNEICARLKAKCVWVENDFDGMIPALKA
KKFDGVLSSMSMTPARAEQIAFSDKLFNTPTRLVAKKGAGLQPSAESLKGKSVGVEQGTIQETYAKAYWAPKGVQVVPYQ
NQDGVYQDLMSGRLDAALQDAVQADLGFLKTPRGANFAFAGGNLDDPKTLGNGAGIGLRKEDADLKAKIDAAIAAMLKDG
TYRKLEKKYFAFDVYGG
>Mature_257_residues
MKKLVLSVALACIAVGAHAKDWSTIRFGVDASYPPFESKDASGKVVGFDVDLGNEICARLKAKCVWVENDFDGMIPALKA
KKFDGVLSSMSMTPARAEQIAFSDKLFNTPTRLVAKKGAGLQPSAESLKGKSVGVEQGTIQETYAKAYWAPKGVQVVPYQ
NQDGVYQDLMSGRLDAALQDAVQADLGFLKTPRGANFAFAGGNLDDPKTLGNGAGIGLRKEDADLKAKIDAAIAAMLKDG
TYRKLEKKYFAFDVYGG

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=54.4401544401544, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=53.8461538461538, Blast_Score=277, Evalue=5e-76,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=36, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=33.2, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=34.1269841269841, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788228, Length=236, Percent_Identity=32.2033898305085, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786876, Length=235, Percent_Identity=28.0851063829787, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27499; Mature: 27499

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLSVALACIAVGAHAKDWSTIRFGVDASYPPFESKDASGKVVGFDVDLGNEICARL
CHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
KAKCVWVENDFDGMIPALKAKKFDGVLSSMSMTPARAEQIAFSDKLFNTPTRLVAKKGAG
HEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCC
LQPSAESLKGKSVGVEQGTIQETYAKAYWAPKGVQVVPYQNQDGVYQDLMSGRLDAALQD
CCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCHHHHHHHH
AVQADLGFLKTPRGANFAFAGGNLDDPKTLGNGAGIGLRKEDADLKAKIDAAIAAMLKDG
HHHHHHHHHCCCCCCCEEEECCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
TYRKLEKKYFAFDVYGG
HHHHHHHHEEEEEECCC
>Mature Secondary Structure
MKKLVLSVALACIAVGAHAKDWSTIRFGVDASYPPFESKDASGKVVGFDVDLGNEICARL
CHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
KAKCVWVENDFDGMIPALKAKKFDGVLSSMSMTPARAEQIAFSDKLFNTPTRLVAKKGAG
HEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCC
LQPSAESLKGKSVGVEQGTIQETYAKAYWAPKGVQVVPYQNQDGVYQDLMSGRLDAALQD
CCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCHHHHHHHH
AVQADLGFLKTPRGANFAFAGGNLDDPKTLGNGAGIGLRKEDADLKAKIDAAIAAMLKDG
HHHHHHHHHCCCCCCCEEEECCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
TYRKLEKKYFAFDVYGG
HHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]