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The map label for this gene is braD [H]

Identifier: 189352198

GI number: 189352198

Start: 235902

End: 237713

Strand: Direct

Name: braD [H]

Synonym: BMULJ_03416

Alternate gene names: 189352198

Gene position: 235902-237713 (Clockwise)

Preceding gene: 189352197

Following gene: 189352199

Centisome position: 9.54

GC content: 70.14

Gene sequence:

>1812_bases
ATGGCATCCTGGCTCGACTACACGCTCAACGGCCTCATCGTCGGCAATATCTACGCGCTGCTCGCCGTCGGGCTCGCGTT
GATCTTCGGCGTGTCGCATCTGATCAACTTCGCGCACGGCTCGGTCTATATGGTCGGCGCGTTCATCGGCTGGCTGTGCC
TGACGCGCTTCGGGCTGCCGCTGCCGGTCGCGCTCGCGGCGGTGCTGCTAGGCTGCGGCGCGCTCGGCGTCGCGATCGAA
CGGATCGGGCTGCGGCCGTTGCGTCATGCGGCGCGCATCGCGCCGCTGCTCGCGACGATCGGCATCAGCTTCATCCTCGA
CCAGCTCGCGCAGCTCGCGTTCGGCGCGGACCCGCGCGCGGTGCCGACGCCGCTGCCCGACTGGCATCTGCGACTCGGCG
GCGCCACGCTCGGGTCGCTCGACCTGCTGATCGCCGGCATCGGCATCGCGGCGGCCGCACTGCTGTACGGCTTCCTGCGC
TTTACGCGGCTCGGCTGGGCCGTGCGCGCGACGGCGCAGGATCGCGACGCGGCGCTGCAGATGGGCGTCGACGTCGATCG
AGTAAACCAGACGGTGTTCGCGATCGCATGCGCGCTCGGCGGCGTGAGCGGGCTGCTGGTCGGCATGTATTACAACAGCA
TCGATCCGGCGATGGGCTTCCAGGCGACGCTAAAGGGCGTCGTCGCGCTGCTGATCGGCGGGCTCGGCAACGTGCCGGGC
GCGATCGCGGGGAGCCTGCTGCTCGGGCTCGTCGAAAGCTACGGCGTCGCGCTGTTCGGCACGAGCTATCGCGACCTGTT
CGCGTTCGGGCTGCTGATCGTGTTTCTCGTCTGGCGGCCGAACGGCTTGTTCAGCGCGAACCGCGCGCTGCCGCCCGAGC
CGATGACGGGCACCTTCCTCGCGGCCGCGAAGGCGCTGCGCGTGCCGCGCCCGGCGATCGTCGCGCTGGTCGCGCTCGCG
GTCGTGCTGCCCTGGCTCGGCGCATCGCCTTACGTGCTGCAGACGCTGACCAACGCGTGGCTGTACGGCCTGCTCGCGCT
GAGCCTCACGCTCGTCGCCGGCACGGTCGGGCAGATCTCGCTCGGCCATGCGGCGCTGCTCGTGATCGGCGCGTATGCGT
CGGCGCTGCTGTCGTCGGATCTCGGCTGGTCGCCGGCCGTGACGATTCCGTGCGCGGGCGCGATCACGGCCGTGCTCGGG
ACGCTGCTCGTCTATCCGGCGTTCCGGCTGCGCGGTCACTACGTGTCGATCGCGACGCTCGGCATCGGCGAAGTGGTGAG
CCTCGTGATCCTGAACTGGGACGGTCTCACGCGCGGCCCGCTCGGCATCACGGGCATCGCACCGCTGCCGTGGGCGTCGA
CCGCACAGGCCGCGTACTGGTTCACGTTGACGGTGCTCGTCGCGTTCGCACTCGTGCAGGTGCGGCTGCTGCGTTCGCAC
CTGGGCCGCACGCTGCGCGCGGTGCGCGAGGACGACGTCGCCGCGCGCGCGCACGGGATCGCACCGAACCGCTACAAGGC
GATCGCGTTCGCGGTTGGCGGTGTCGCGGCGGGCGTGAGCGGCGGTATTGCGGCACACCTGTACAGCTACATCAATCACC
AGACCTTCGATTCGCAGGTGTCGATCCTCGCGCTGACGATGGTGATCCTCGGCGGGCTCGGCAACGTGCTCGGCGGCATC
GCGGGCGCGCTCGCGCTGATCGGCCTGCCCGAACTGTTCCGCTGGGCGGCCGACTACCGGATGCTGATCTACGGTCTCGT
GCTGCTGCTGCTCGTCCGGTTCCGGCCGCAGGGCTTGCTCGGCACCGTGTGA

Upstream 100 bases:

>100_bases
CGCGCACCGTGTATCTCGTCGTGAAGCAGGGCCGGTGGGCGCTGTGGGACGGCGCCCGGCCGCAGCTCGCGGCACGCTGA
ACCGTACCGGACGCATCGCA

Downstream 100 bases:

>100_bases
GGAGGAATGCGATGACCACCACCCAACCGCCCTTGCTCGACGTGCAAGGGTTGACGCGCCGCTTCGATGGCGTCACGGCG
CTCGACGGTGCGAGCCTGAC

Product: branched-chain amino acid ABC-type transport system permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MASWLDYTLNGLIVGNIYALLAVGLALIFGVSHLINFAHGSVYMVGAFIGWLCLTRFGLPLPVALAAVLLGCGALGVAIE
RIGLRPLRHAARIAPLLATIGISFILDQLAQLAFGADPRAVPTPLPDWHLRLGGATLGSLDLLIAGIGIAAAALLYGFLR
FTRLGWAVRATAQDRDAALQMGVDVDRVNQTVFAIACALGGVSGLLVGMYYNSIDPAMGFQATLKGVVALLIGGLGNVPG
AIAGSLLLGLVESYGVALFGTSYRDLFAFGLLIVFLVWRPNGLFSANRALPPEPMTGTFLAAAKALRVPRPAIVALVALA
VVLPWLGASPYVLQTLTNAWLYGLLALSLTLVAGTVGQISLGHAALLVIGAYASALLSSDLGWSPAVTIPCAGAITAVLG
TLLVYPAFRLRGHYVSIATLGIGEVVSLVILNWDGLTRGPLGITGIAPLPWASTAQAAYWFTLTVLVAFALVQVRLLRSH
LGRTLRAVREDDVAARAHGIAPNRYKAIAFAVGGVAAGVSGGIAAHLYSYINHQTFDSQVSILALTMVILGGLGNVLGGI
AGALALIGLPELFRWAADYRMLIYGLVLLLLVRFRPQGLLGTV

Sequences:

>Translated_603_residues
MASWLDYTLNGLIVGNIYALLAVGLALIFGVSHLINFAHGSVYMVGAFIGWLCLTRFGLPLPVALAAVLLGCGALGVAIE
RIGLRPLRHAARIAPLLATIGISFILDQLAQLAFGADPRAVPTPLPDWHLRLGGATLGSLDLLIAGIGIAAAALLYGFLR
FTRLGWAVRATAQDRDAALQMGVDVDRVNQTVFAIACALGGVSGLLVGMYYNSIDPAMGFQATLKGVVALLIGGLGNVPG
AIAGSLLLGLVESYGVALFGTSYRDLFAFGLLIVFLVWRPNGLFSANRALPPEPMTGTFLAAAKALRVPRPAIVALVALA
VVLPWLGASPYVLQTLTNAWLYGLLALSLTLVAGTVGQISLGHAALLVIGAYASALLSSDLGWSPAVTIPCAGAITAVLG
TLLVYPAFRLRGHYVSIATLGIGEVVSLVILNWDGLTRGPLGITGIAPLPWASTAQAAYWFTLTVLVAFALVQVRLLRSH
LGRTLRAVREDDVAARAHGIAPNRYKAIAFAVGGVAAGVSGGIAAHLYSYINHQTFDSQVSILALTMVILGGLGNVLGGI
AGALALIGLPELFRWAADYRMLIYGLVLLLLVRFRPQGLLGTV
>Mature_602_residues
ASWLDYTLNGLIVGNIYALLAVGLALIFGVSHLINFAHGSVYMVGAFIGWLCLTRFGLPLPVALAAVLLGCGALGVAIER
IGLRPLRHAARIAPLLATIGISFILDQLAQLAFGADPRAVPTPLPDWHLRLGGATLGSLDLLIAGIGIAAAALLYGFLRF
TRLGWAVRATAQDRDAALQMGVDVDRVNQTVFAIACALGGVSGLLVGMYYNSIDPAMGFQATLKGVVALLIGGLGNVPGA
IAGSLLLGLVESYGVALFGTSYRDLFAFGLLIVFLVWRPNGLFSANRALPPEPMTGTFLAAAKALRVPRPAIVALVALAV
VLPWLGASPYVLQTLTNAWLYGLLALSLTLVAGTVGQISLGHAALLVIGAYASALLSSDLGWSPAVTIPCAGAITAVLGT
LLVYPAFRLRGHYVSIATLGIGEVVSLVILNWDGLTRGPLGITGIAPLPWASTAQAAYWFTLTVLVAFALVQVRLLRSHL
GRTLRAVREDDVAARAHGIAPNRYKAIAFAVGGVAAGVSGGIAAHLYSYINHQTFDSQVSILALTMVILGGLGNVLGGIA
GALALIGLPELFRWAADYRMLIYGLVLLLLVRFRPQGLLGTV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=292, Percent_Identity=33.2191780821918, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789865, Length=287, Percent_Identity=34.8432055749129, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 62876; Mature: 62745

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASWLDYTLNGLIVGNIYALLAVGLALIFGVSHLINFAHGSVYMVGAFIGWLCLTRFGLP
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
LPVALAAVLLGCGALGVAIERIGLRPLRHAARIAPLLATIGISFILDQLAQLAFGADPRA
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPTPLPDWHLRLGGATLGSLDLLIAGIGIAAAALLYGFLRFTRLGWAVRATAQDRDAALQ
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHH
MGVDVDRVNQTVFAIACALGGVSGLLVGMYYNSIDPAMGFQATLKGVVALLIGGLGNVPG
HCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCH
AIAGSLLLGLVESYGVALFGTSYRDLFAFGLLIVFLVWRPNGLFSANRALPPEPMTGTFL
HHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
AAAKALRVPRPAIVALVALAVVLPWLGASPYVLQTLTNAWLYGLLALSLTLVAGTVGQIS
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LGHAALLVIGAYASALLSSDLGWSPAVTIPCAGAITAVLGTLLVYPAFRLRGHYVSIATL
HHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHH
GIGEVVSLVILNWDGLTRGPLGITGIAPLPWASTAQAAYWFTLTVLVAFALVQVRLLRSH
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGRTLRAVREDDVAARAHGIAPNRYKAIAFAVGGVAAGVSGGIAAHLYSYINHQTFDSQV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
SILALTMVILGGLGNVLGGIAGALALIGLPELFRWAADYRMLIYGLVLLLLVRFRPQGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTV
CCC
>Mature Secondary Structure 
ASWLDYTLNGLIVGNIYALLAVGLALIFGVSHLINFAHGSVYMVGAFIGWLCLTRFGLP
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
LPVALAAVLLGCGALGVAIERIGLRPLRHAARIAPLLATIGISFILDQLAQLAFGADPRA
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPTPLPDWHLRLGGATLGSLDLLIAGIGIAAAALLYGFLRFTRLGWAVRATAQDRDAALQ
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHH
MGVDVDRVNQTVFAIACALGGVSGLLVGMYYNSIDPAMGFQATLKGVVALLIGGLGNVPG
HCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCH
AIAGSLLLGLVESYGVALFGTSYRDLFAFGLLIVFLVWRPNGLFSANRALPPEPMTGTFL
HHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
AAAKALRVPRPAIVALVALAVVLPWLGASPYVLQTLTNAWLYGLLALSLTLVAGTVGQIS
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LGHAALLVIGAYASALLSSDLGWSPAVTIPCAGAITAVLGTLLVYPAFRLRGHYVSIATL
HHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHH
GIGEVVSLVILNWDGLTRGPLGITGIAPLPWASTAQAAYWFTLTVLVAFALVQVRLLRSH
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGRTLRAVREDDVAARAHGIAPNRYKAIAFAVGGVAAGVSGGIAAHLYSYINHQTFDSQV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
SILALTMVILGGLGNVLGGIAGALALIGLPELFRWAADYRMLIYGLVLLLLVRFRPQGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]