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The map label for this gene is livK [C]

Identifier: 189352197

GI number: 189352197

Start: 234688

End: 235881

Strand: Direct

Name: livK [C]

Synonym: BMULJ_03415

Alternate gene names: 189352197

Gene position: 234688-235881 (Clockwise)

Preceding gene: 189352192

Following gene: 189352198

Centisome position: 9.49

GC content: 69.18

Gene sequence:

>1194_bases
ATGAACGTTTCGGGGAACGACGCGCGCGGCGCATGGCGGCGCGTGCTGGGCACGGTGGCGGCGCTCGCGGCCGCCTGGGG
CTTGTCGACGGGCGCCGCGCTCGCGGCAGGCGTCTCGGGCGAGCCGGTCGTCGTCGGCGTGAGCGGGCCGCTCACGGGGC
AGGACGCGCAATACGGCGAGCAGTGGAAGCGCGGCTTCGACCTCGCACTCGACGAGATCAACGGCAGCGGCGGCATACAC
GGCCGGCCGCTCGCGATCGACTTCCAGGACAGCCGCAGCGATCCGCGCCAGGCGGTGGCGATCGCGCAGAAGTTCGTCGC
CGATCCGCGCGTCGTGATCGAGCTCGGCGATTTTTCGAGCGCGACGTCGATGGCCGCGTCGCCGATTTATCAGCGCGGTC
AACTGATCCAGTTCGGCTTCACGAATTCGCACCCGGACTTCACGAAGGGCGGCGACTATCTGTGGAGTACCGCGCTGAGC
CAGGCCGAGGAACAGCCGCTGCTCGCGCGCTACGCCGTGAAGGAACTCGGCTTCAAACGGATCGCGGTGCTGTATCTGAA
CACCGACTGGGGCCGCACGAGCAAGGACATCTTCGCGAAGGCGGCGGCAGCGCTCGGTGCGCAGGTCGTCGCGGCCGAAG
GCTATCAACCGGCCGAAAAGGATTTCCGCTCGACGCTCGTGCGGATCGGCGAGTCGAGGCCCGACTCGATCGTGCTGATC
TCGTATTACGCGGACGGCGCGCAGATCGTGCGGCAGGCGCGCACCTCGGGCATTGCGCTGCCGATCGCGGCGGTCGGCTC
CGTGTACTCGCCGAAATTCCTCGAACTGGGCGGCACGGCCGTCGACGGCGTCTACACCGAATCGAATTTCTTCCCGGCCG
AACCGCGCCCCGAAGTGCAGGCGTTCGTGCAGCGCTATCGCGCGAAATTCCACGCCGACCCCGACTCGTTCGTCGCGCGC
GCCTACGACGCGCTGATCCTGTCGGCCGAGGTGCTGCGCCGCTATGGCACGACGCGCCAGGCCGCGCACGACGGCTTCGC
GAAGATCAGCGACGTGCCGAGCGTGATCTTCGGCAAGGTGCGCTTCGATCCGCAGACGCGCCGCGTCGCGGGCGCGCGCA
CCGTGTATCTCGTCGTGAAGCAGGGCCGGTGGGCGCTGTGGGACGGCGCCCGGCCGCAGCTCGCGGCACGCTGA

Upstream 100 bases:

>100_bases
GTGCGCCGCGATATCGATATGCGCAAAACTTCGTTGGATCGTCCGGACCCCGATGGTTTCATGCCCTCACTCTTTTCGAT
TGACTGACGTTGGGGGATCC

Downstream 100 bases:

>100_bases
ACCGTACCGGACGCATCGCAATGGCATCCTGGCTCGACTACACGCTCAACGGCCTCATCGTCGGCAATATCTACGCGCTG
CTCGCCGTCGGGCTCGCGTT

Product: branched-chain amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNVSGNDARGAWRRVLGTVAALAAAWGLSTGAALAAGVSGEPVVVGVSGPLTGQDAQYGEQWKRGFDLALDEINGSGGIH
GRPLAIDFQDSRSDPRQAVAIAQKFVADPRVVIELGDFSSATSMAASPIYQRGQLIQFGFTNSHPDFTKGGDYLWSTALS
QAEEQPLLARYAVKELGFKRIAVLYLNTDWGRTSKDIFAKAAAALGAQVVAAEGYQPAEKDFRSTLVRIGESRPDSIVLI
SYYADGAQIVRQARTSGIALPIAAVGSVYSPKFLELGGTAVDGVYTESNFFPAEPRPEVQAFVQRYRAKFHADPDSFVAR
AYDALILSAEVLRRYGTTRQAAHDGFAKISDVPSVIFGKVRFDPQTRRVAGARTVYLVVKQGRWALWDGARPQLAAR

Sequences:

>Translated_397_residues
MNVSGNDARGAWRRVLGTVAALAAAWGLSTGAALAAGVSGEPVVVGVSGPLTGQDAQYGEQWKRGFDLALDEINGSGGIH
GRPLAIDFQDSRSDPRQAVAIAQKFVADPRVVIELGDFSSATSMAASPIYQRGQLIQFGFTNSHPDFTKGGDYLWSTALS
QAEEQPLLARYAVKELGFKRIAVLYLNTDWGRTSKDIFAKAAAALGAQVVAAEGYQPAEKDFRSTLVRIGESRPDSIVLI
SYYADGAQIVRQARTSGIALPIAAVGSVYSPKFLELGGTAVDGVYTESNFFPAEPRPEVQAFVQRYRAKFHADPDSFVAR
AYDALILSAEVLRRYGTTRQAAHDGFAKISDVPSVIFGKVRFDPQTRRVAGARTVYLVVKQGRWALWDGARPQLAAR
>Mature_397_residues
MNVSGNDARGAWRRVLGTVAALAAAWGLSTGAALAAGVSGEPVVVGVSGPLTGQDAQYGEQWKRGFDLALDEINGSGGIH
GRPLAIDFQDSRSDPRQAVAIAQKFVADPRVVIELGDFSSATSMAASPIYQRGQLIQFGFTNSHPDFTKGGDYLWSTALS
QAEEQPLLARYAVKELGFKRIAVLYLNTDWGRTSKDIFAKAAAALGAQVVAAEGYQPAEKDFRSTLVRIGESRPDSIVLI
SYYADGAQIVRQARTSGIALPIAAVGSVYSPKFLELGGTAVDGVYTESNFFPAEPRPEVQAFVQRYRAKFHADPDSFVAR
AYDALILSAEVLRRYGTTRQAAHDGFAKISDVPSVIFGKVRFDPQTRRVAGARTVYLVVKQGRWALWDGARPQLAAR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=292, Percent_Identity=28.4246575342466, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789867, Length=308, Percent_Identity=26.2987012987013, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42762; Mature: 42762

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSGNDARGAWRRVLGTVAALAAAWGLSTGAALAAGVSGEPVVVGVSGPLTGQDAQYGE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCCCHHHH
QWKRGFDLALDEINGSGGIHGRPLAIDFQDSRSDPRQAVAIAQKFVADPRVVIELGDFSS
HHHHCHHHEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
ATSMAASPIYQRGQLIQFGFTNSHPDFTKGGDYLWSTALSQAEEQPLLARYAVKELGFKR
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
IAVLYLNTDWGRTSKDIFAKAAAALGAQVVAAEGYQPAEKDFRSTLVRIGESRPDSIVLI
EEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
SYYADGAQIVRQARTSGIALPIAAVGSVYSPKFLELGGTAVDGVYTESNFFPAEPRPEVQ
EEECCHHHHHHHHHHCCCEEHHHHHCCCCCCCCEECCCCEECCEEECCCCCCCCCCHHHH
AFVQRYRAKFHADPDSFVARAYDALILSAEVLRRYGTTRQAAHDGFAKISDVPSVIFGKV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCCHHHEEEE
RFDPQTRRVAGARTVYLVVKQGRWALWDGARPQLAAR
EECCHHHHHCCCEEEEEEEECCCEEEECCCCCCCCCC
>Mature Secondary Structure
MNVSGNDARGAWRRVLGTVAALAAAWGLSTGAALAAGVSGEPVVVGVSGPLTGQDAQYGE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCCCCHHHH
QWKRGFDLALDEINGSGGIHGRPLAIDFQDSRSDPRQAVAIAQKFVADPRVVIELGDFSS
HHHHCHHHEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
ATSMAASPIYQRGQLIQFGFTNSHPDFTKGGDYLWSTALSQAEEQPLLARYAVKELGFKR
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
IAVLYLNTDWGRTSKDIFAKAAAALGAQVVAAEGYQPAEKDFRSTLVRIGESRPDSIVLI
EEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
SYYADGAQIVRQARTSGIALPIAAVGSVYSPKFLELGGTAVDGVYTESNFFPAEPRPEVQ
EEECCHHHHHHHHHHCCCEEHHHHHCCCCCCCCEECCCCEECCEEECCCCCCCCCCHHHH
AFVQRYRAKFHADPDSFVARAYDALILSAEVLRRYGTTRQAAHDGFAKISDVPSVIFGKV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCCHHHEEEE
RFDPQTRRVAGARTVYLVVKQGRWALWDGARPQLAAR
EECCHHHHHCCCEEEEEEEECCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA