| Definition | Burkholderia multivorans ATCC 17616 chromosome 2, complete genome. |
|---|---|
| Accession | NC_010805 |
| Length | 2,473,162 |
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The map label for this gene is dnaK [H]
Identifier: 189352192
GI number: 189352192
Start: 227717
End: 230473
Strand: Direct
Name: dnaK [H]
Synonym: BMULJ_03410
Alternate gene names: 189352192
Gene position: 227717-230473 (Clockwise)
Preceding gene: 189352191
Following gene: 189352197
Centisome position: 9.21
GC content: 71.93
Gene sequence:
>2757_bases ATGAAGCGCTATACGGTCGGCATCGACCTCGGCACCAGCAATACGGTCGTCGCCTACGTCGAGCCCGGCTCGGACGCGAT TCGCGTGTTCGACGTCGAGCAACTCGTCGGCGCCGGCGCGGTCGCCGCGCAGCCGCTGCTGCCGTCGGTGCGCTATCACC CCGCGCCCGGCGAACTGGCGGCGGACGCGTTGCGGCTGCCGTGGCAGGGCGCCGGCGCGGCGGCGCAGGCCGGCCGCGAC GATGCGCCGTCGCCCGTGATCGGGCGGTATGCGCGCACGCTTGGCGCGCAGGTGCCGGGCCGCCTCGTCACGAGCGCGAA GAGCTGGCTGTCGCACGCGTCGGTCGATCGCCTCGCGCCGATTCTGCCGTGGGGCGCTGCCGAGGGCGTCGACAAGGTGT CGCCCGTGGATGCGAGCGCGAGCTATCTCGCGCACGTGCGTGCCGCATGGGATGCGCGCTTTCCCGACGCGCCGCTCGCG CAACAGCATGTCATCCTGACCGTCCCCGCTTCGTTCGACGACGGCGCGCGCGCGCTGACCGTCGAGGCCGCGCGACGCGC GGGGCTGCCTGCGCTGCGGCTGCTCGAGGAGCCGCAGGCCGCGTTCTACGACTGGCTCTACGGCCAGCGTGCGACGCTGC GCGACACGTTCGCCGCGCCGCGCCGCGTGCTGATCTGCGACGTCGGCGGCGGCACGACCGATCTGACGCTCGTCGACGTC GCGCCCGGCGACGATGGCGAGCCGACGTTTACGCGCGTCGGCGTCGGCAATCATCTGATGCTCGGCGGCGACAACATGGA CCTCGCGCTCGCGCGCCTCGTCGAGACGCGCTTGACCGAGCCCGGCACGCGGCTGTCGGCCGCGAGCCTGTCGCAGCTCG TCGAGCGTTGCCGCGCGGCGAAGGAGCGCCTGCTCGGCGACGAGGCGCCCGAATCGGTGACCGTCACGCTGCTCGGTGCC GGCAGCCGGCTCGTCGGCGGCGCGCGATCGGCCGAGTTGACGCGTCGCGAGGTCGAGCAGATCGTCGTCGATGGCTTTTT TCCGCAAGTCGCGGCCGACGAGCTGCCGCAGCGTGCGCGTGCGGCGATCGTCGAATTCGGCTTGCCGTACGCGAGCGATC CGGCCGTCACGCGGCACGTCGCCGCGTTCCTGAAGCGTCACGCGGAAGGTCCGCTGCCCGACACGCTGCTGCTCAACGGC GGCGTGTTTCGTGCGCACGCGCTGGCGGGCCGCCTCGCGCAGACGCTCGGTGCGTGGCGCGGCGCGCCGCTCGACGTGCT GCACAACGCGCATCCGGACGTCGCGGTCGCGCGCGGTGCGGTCGCATACGGTCTCGCCCGCGCCGGGCATGCGCCACGCA TCGGCGGCGGCTCGGCGCGCAGCTATTTCCTCGTGCTCGACGACGGCGCGGCCGACGGGCGGCCGCGCGGCGTGTGCCTG CTGCCGCGCGGGGCGGAAGAGGGCCGCGAGATCCGGCTCGAGGATCGCACGTTCGCGCTGCAGCTCGGGCAGCCGGTGCG CTTTCACCTGATGTCGACAGTTACCGATACCGCGTACCGGCCGGGCGATCTCGTCGATCTCGACGGCGGCGACTTCGTGC GGCTGCCGCCGATCGCGACCGTCGTCGACGGGCAGGCCGACGGCGGCGCGCGCGAGACGCCGGTGAAGCTGACCGCATCG CTGACCGAAGTCGGCACGCTCGAAATGCACTGCATCGCGACCGACGATGCCGCGCGCCGCTGGCGCCTCGAATTCCAGCT GCGCGGCGACGCGCCGGCACAGCAGGACGGCGCCGCACCTGCGCGGCATCCGCGTATCGATCAGGCGCTCGAACTGATCG ACCGCTCGTTCGGCAGCCGCGCGGCGAACGTGACGCCGAAGGATACGAGACGGTTGCGTGCGCAACTTGAACAGGTACTT GGTCCGCGCGAAGAATGGGACGTCGCGCTCGCGCGCGAGCTGTTCGACGCATTGCTCGCGCGGGCGCGCCGACGCCGGCG CTCGGCCGATCACGAACGGGCATGGCTCAATCTCGCCGGCTATTGCGTGCGGCCGGGCTTCGGTCATCCGCTCGATGCCT GGCGCATCGAACAGCTGTGGCCGCTGTTCGACGACGGCATCCAGTACGTGAACGACGCGCAGGTATGGTCCGAATGGTGG ACGCTGTGGCGGCGTGCGGCCGGCGGCCTCGACGACGACGCGCAGATGCAGGTGCGCGACGCGATCGCGTTTCTCGAGCC GTCGCCCGACGACAAGCGACGCAAGCTGCCGTTCGATCCCGACAAGGTGGGGCCCGCCGACATGATGCGGTTGTCGGCGT CGCTCGAACGGCTGCCGATCGAACGCAAGATCGAGCTCGGCGAACGGCTGATCGCGCCTGTGCAGAAGCCGGCCGAGCGC GCGCTGCGCGCCTGGGCGCTCGGGCGCGTCGGCGCGCGGCGTCCATTCTACGGCAGCGCGCACAACGTCGTGCCGGCCGA CGTCGCGTGCCGGTGGCTCGACGCGCTGTTCGCGCTCGACTGGAAACAGGTCGAGCCTGCCGCGTTTGCCGCCGCGCAGA TCGCGCAGCTGACGGGCGACCGCGAACGCGATCTGCCGGACGACACGCGCGACGCGGTGATCCGGCGGCTGTCGGCCGCG AACGTGTCGCAGACGTGGATCGATCGTGTACGCGAGGTCGTCGCGTTCGATGAGGCGGATACGGTGCGGGTGTTCGGTGA GACGTTGCCGGCGGGGTTGAAGTTGTTGGGGGGGTGA
Upstream 100 bases:
>100_bases GCGTGACCGAGGCCGGTACGCTCGAGCTCGAAGCGATCCCGAGCGGCACGGACGAGCGCTGGAAGGTCGAGTTCGACGTG CGCGGCACCGCTTGAGCGTA
Downstream 100 bases:
>100_bases GGGGCGGTGGGGCAGTATCGCGGCGAGATGCGCGTCCGCGGAACTGCCGGCGACGACGATCCGGGAGCGGCCATTGAACG GAGCGGCCATTCGCGGGCGA
Product: putative molecular chaperone
Products: NA
Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]
Number of amino acids: Translated: 918; Mature: 918
Protein sequence:
>918_residues MKRYTVGIDLGTSNTVVAYVEPGSDAIRVFDVEQLVGAGAVAAQPLLPSVRYHPAPGELAADALRLPWQGAGAAAQAGRD DAPSPVIGRYARTLGAQVPGRLVTSAKSWLSHASVDRLAPILPWGAAEGVDKVSPVDASASYLAHVRAAWDARFPDAPLA QQHVILTVPASFDDGARALTVEAARRAGLPALRLLEEPQAAFYDWLYGQRATLRDTFAAPRRVLICDVGGGTTDLTLVDV APGDDGEPTFTRVGVGNHLMLGGDNMDLALARLVETRLTEPGTRLSAASLSQLVERCRAAKERLLGDEAPESVTVTLLGA GSRLVGGARSAELTRREVEQIVVDGFFPQVAADELPQRARAAIVEFGLPYASDPAVTRHVAAFLKRHAEGPLPDTLLLNG GVFRAHALAGRLAQTLGAWRGAPLDVLHNAHPDVAVARGAVAYGLARAGHAPRIGGGSARSYFLVLDDGAADGRPRGVCL LPRGAEEGREIRLEDRTFALQLGQPVRFHLMSTVTDTAYRPGDLVDLDGGDFVRLPPIATVVDGQADGGARETPVKLTAS LTEVGTLEMHCIATDDAARRWRLEFQLRGDAPAQQDGAAPARHPRIDQALELIDRSFGSRAANVTPKDTRRLRAQLEQVL GPREEWDVALARELFDALLARARRRRRSADHERAWLNLAGYCVRPGFGHPLDAWRIEQLWPLFDDGIQYVNDAQVWSEWW TLWRRAAGGLDDDAQMQVRDAIAFLEPSPDDKRRKLPFDPDKVGPADMMRLSASLERLPIERKIELGERLIAPVQKPAER ALRAWALGRVGARRPFYGSAHNVVPADVACRWLDALFALDWKQVEPAAFAAAQIAQLTGDRERDLPDDTRDAVIRRLSAA NVSQTWIDRVREVVAFDEADTVRVFGETLPAGLKLLGG
Sequences:
>Translated_918_residues MKRYTVGIDLGTSNTVVAYVEPGSDAIRVFDVEQLVGAGAVAAQPLLPSVRYHPAPGELAADALRLPWQGAGAAAQAGRD DAPSPVIGRYARTLGAQVPGRLVTSAKSWLSHASVDRLAPILPWGAAEGVDKVSPVDASASYLAHVRAAWDARFPDAPLA QQHVILTVPASFDDGARALTVEAARRAGLPALRLLEEPQAAFYDWLYGQRATLRDTFAAPRRVLICDVGGGTTDLTLVDV APGDDGEPTFTRVGVGNHLMLGGDNMDLALARLVETRLTEPGTRLSAASLSQLVERCRAAKERLLGDEAPESVTVTLLGA GSRLVGGARSAELTRREVEQIVVDGFFPQVAADELPQRARAAIVEFGLPYASDPAVTRHVAAFLKRHAEGPLPDTLLLNG GVFRAHALAGRLAQTLGAWRGAPLDVLHNAHPDVAVARGAVAYGLARAGHAPRIGGGSARSYFLVLDDGAADGRPRGVCL LPRGAEEGREIRLEDRTFALQLGQPVRFHLMSTVTDTAYRPGDLVDLDGGDFVRLPPIATVVDGQADGGARETPVKLTAS LTEVGTLEMHCIATDDAARRWRLEFQLRGDAPAQQDGAAPARHPRIDQALELIDRSFGSRAANVTPKDTRRLRAQLEQVL GPREEWDVALARELFDALLARARRRRRSADHERAWLNLAGYCVRPGFGHPLDAWRIEQLWPLFDDGIQYVNDAQVWSEWW TLWRRAAGGLDDDAQMQVRDAIAFLEPSPDDKRRKLPFDPDKVGPADMMRLSASLERLPIERKIELGERLIAPVQKPAER ALRAWALGRVGARRPFYGSAHNVVPADVACRWLDALFALDWKQVEPAAFAAAQIAQLTGDRERDLPDDTRDAVIRRLSAA NVSQTWIDRVREVVAFDEADTVRVFGETLPAGLKLLGG >Mature_918_residues MKRYTVGIDLGTSNTVVAYVEPGSDAIRVFDVEQLVGAGAVAAQPLLPSVRYHPAPGELAADALRLPWQGAGAAAQAGRD DAPSPVIGRYARTLGAQVPGRLVTSAKSWLSHASVDRLAPILPWGAAEGVDKVSPVDASASYLAHVRAAWDARFPDAPLA QQHVILTVPASFDDGARALTVEAARRAGLPALRLLEEPQAAFYDWLYGQRATLRDTFAAPRRVLICDVGGGTTDLTLVDV APGDDGEPTFTRVGVGNHLMLGGDNMDLALARLVETRLTEPGTRLSAASLSQLVERCRAAKERLLGDEAPESVTVTLLGA GSRLVGGARSAELTRREVEQIVVDGFFPQVAADELPQRARAAIVEFGLPYASDPAVTRHVAAFLKRHAEGPLPDTLLLNG GVFRAHALAGRLAQTLGAWRGAPLDVLHNAHPDVAVARGAVAYGLARAGHAPRIGGGSARSYFLVLDDGAADGRPRGVCL LPRGAEEGREIRLEDRTFALQLGQPVRFHLMSTVTDTAYRPGDLVDLDGGDFVRLPPIATVVDGQADGGARETPVKLTAS LTEVGTLEMHCIATDDAARRWRLEFQLRGDAPAQQDGAAPARHPRIDQALELIDRSFGSRAANVTPKDTRRLRAQLEQVL GPREEWDVALARELFDALLARARRRRRSADHERAWLNLAGYCVRPGFGHPLDAWRIEQLWPLFDDGIQYVNDAQVWSEWW TLWRRAAGGLDDDAQMQVRDAIAFLEPSPDDKRRKLPFDPDKVGPADMMRLSASLERLPIERKIELGERLIAPVQKPAER ALRAWALGRVGARRPFYGSAHNVVPADVACRWLDALFALDWKQVEPAAFAAAQIAQLTGDRERDLPDDTRDAVIRRLSAA NVSQTWIDRVREVVAFDEADTVRVFGETLPAGLKLLGG
Specific function: Acts as a chaperone [H]
COG id: COG0443
COG function: function code O; Molecular chaperone
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the heat shock protein 70 family [H]
Homologues:
Organism=Escherichia coli, GI1788875, Length=284, Percent_Identity=29.5774647887324, Blast_Score=78, Evalue=3e-15, Organism=Escherichia coli, GI1786196, Length=181, Percent_Identity=30.3867403314917, Blast_Score=65, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17507981, Length=348, Percent_Identity=25.5747126436782, Blast_Score=69, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6319972, Length=338, Percent_Identity=26.3313609467456, Blast_Score=70, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6324120, Length=338, Percent_Identity=26.3313609467456, Blast_Score=70, Evalue=1e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012725 - InterPro: IPR018181 - InterPro: IPR001023 - InterPro: IPR013126 [H]
Pfam domain/function: PF00012 HSP70 [H]
EC number: NA
Molecular weight: Translated: 99472; Mature: 99472
Theoretical pI: Translated: 6.30; Mature: 6.30
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 0.9 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKRYTVGIDLGTSNTVVAYVEPGSDAIRVFDVEQLVGAGAVAAQPLLPSVRYHPAPGELA CCEEEEEEEECCCCEEEEEEECCCCEEEEEEHHHHHCCCHHHHCCCCCCCCCCCCCCHHH ADALRLPWQGAGAAAQAGRDDAPSPVIGRYARTLGAQVPGRLVTSAKSWLSHASVDRLAP HHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC ILPWGAAEGVDKVSPVDASASYLAHVRAAWDARFPDAPLAQQHVILTVPASFDDGARALT CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEE VEAARRAGLPALRLLEEPQAAFYDWLYGQRATLRDTFAAPRRVLICDVGGGTTDLTLVDV HHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCCEEEEEEE APGDDGEPTFTRVGVGNHLMLGGDNMDLALARLVETRLTEPGTRLSAASLSQLVERCRAA CCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH KERLLGDEAPESVTVTLLGAGSRLVGGARSAELTRREVEQIVVDGFFPQVAADELPQRAR HHHHCCCCCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH AAIVEFGLPYASDPAVTRHVAAFLKRHAEGPLPDTLLLNGGVFRAHALAGRLAQTLGAWR HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCC GAPLDVLHNAHPDVAVARGAVAYGLARAGHAPRIGGGSARSYFLVLDDGAADGRPRGVCL CCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEE LPRGAEEGREIRLEDRTFALQLGQPVRFHLMSTVTDTAYRPGDLVDLDGGDFVRLPPIAT ECCCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCEEE VVDGQADGGARETPVKLTASLTEVGTLEMHCIATDDAARRWRLEFQLRGDAPAQQDGAAP EECCCCCCCCCCCCEEEEEHHHHCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCC ARHPRIDQALELIDRSFGSRAANVTPKDTRRLRAQLEQVLGPREEWDVALARELFDALLA CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH RARRRRRSADHERAWLNLAGYCVRPGFGHPLDAWRIEQLWPLFDDGIQYVNDAQVWSEWW HHHHHHCCCCHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH TLWRRAAGGLDDDAQMQVRDAIAFLEPSPDDKRRKLPFDPDKVGPADMMRLSASLERLPI HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC ERKIELGERLIAPVQKPAERALRAWALGRVGARRPFYGSAHNVVPADVACRWLDALFALD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC WKQVEPAAFAAAQIAQLTGDRERDLPDDTRDAVIRRLSAANVSQTWIDRVREVVAFDEAD HHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC TVRVFGETLPAGLKLLGG HHHHHHHHHCHHHHCCCC >Mature Secondary Structure MKRYTVGIDLGTSNTVVAYVEPGSDAIRVFDVEQLVGAGAVAAQPLLPSVRYHPAPGELA CCEEEEEEEECCCCEEEEEEECCCCEEEEEEHHHHHCCCHHHHCCCCCCCCCCCCCCHHH ADALRLPWQGAGAAAQAGRDDAPSPVIGRYARTLGAQVPGRLVTSAKSWLSHASVDRLAP HHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC ILPWGAAEGVDKVSPVDASASYLAHVRAAWDARFPDAPLAQQHVILTVPASFDDGARALT CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEE VEAARRAGLPALRLLEEPQAAFYDWLYGQRATLRDTFAAPRRVLICDVGGGTTDLTLVDV HHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCCEEEEEEE APGDDGEPTFTRVGVGNHLMLGGDNMDLALARLVETRLTEPGTRLSAASLSQLVERCRAA CCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH KERLLGDEAPESVTVTLLGAGSRLVGGARSAELTRREVEQIVVDGFFPQVAADELPQRAR HHHHCCCCCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH AAIVEFGLPYASDPAVTRHVAAFLKRHAEGPLPDTLLLNGGVFRAHALAGRLAQTLGAWR HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCC GAPLDVLHNAHPDVAVARGAVAYGLARAGHAPRIGGGSARSYFLVLDDGAADGRPRGVCL CCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEE LPRGAEEGREIRLEDRTFALQLGQPVRFHLMSTVTDTAYRPGDLVDLDGGDFVRLPPIAT ECCCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCEEE VVDGQADGGARETPVKLTASLTEVGTLEMHCIATDDAARRWRLEFQLRGDAPAQQDGAAP EECCCCCCCCCCCCEEEEEHHHHCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCC ARHPRIDQALELIDRSFGSRAANVTPKDTRRLRAQLEQVLGPREEWDVALARELFDALLA CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH RARRRRRSADHERAWLNLAGYCVRPGFGHPLDAWRIEQLWPLFDDGIQYVNDAQVWSEWW HHHHHHCCCCHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH TLWRRAAGGLDDDAQMQVRDAIAFLEPSPDDKRRKLPFDPDKVGPADMMRLSASLERLPI HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC ERKIELGERLIAPVQKPAERALRAWALGRVGARRPFYGSAHNVVPADVACRWLDALFALD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC WKQVEPAAFAAAQIAQLTGDRERDLPDDTRDAVIRRLSAANVSQTWIDRVREVVAFDEAD HHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC TVRVFGETLPAGLKLLGG HHHHHHHHHCHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12835416 [H]