Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 189184761

GI number: 189184761

Start: 1884742

End: 1886178

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_1854

Alternate gene names: 189184761

Gene position: 1886178-1884742 (Counterclockwise)

Preceding gene: 189184762

Following gene: 189184760

Centisome position: 93.89

GC content: 32.08

Gene sequence:

>1437_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGGGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTATGAACCATTACATGGTAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGCGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTCGCATTTGAG
GAAATAGGTGGAGTGGATTATCTAGCTAAACTTACAACGTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGCGGCGTTATTTAATTGAAATTGGAGAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATTTAGCTATAACTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGGTTCAAGAGGAACTTTAAGTAAAGGA
TTTATAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCAGCTGCTATTAAAAATAAAAACTCTATTAATGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGGC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTCTTACTCAAAAAAATACTAAA
GATAATGTAGTGCCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCTACTCGAATTCTTTCTATAGA
ATCAGAAATTAATAGCTCTACATTATTTAATGGTAAAATAGGTGAACAAGATGTTGATAAGTTGAAGACTGTACAAAATA
AAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCGATATCTGCAATTAGATCACGAGCCCGTAGACTT
AAACGTACTCATAATTTAGCAATATTATTTATTGATTATTTACAGCTAATAAAAATTGATTCCAGAGGAAGTCAGTATAA
TCGAGTGCAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCAAAAGAACTCAATATTCCAGTCATTGCACTAT
CTCAATTGTCTAGAGCTGTAGAACAAAGGTTAGATAAAAAGCCTATTCTCTCAGATCTAAGAGAATCAGGCTCAATTGAA
CAGGACGCCGATATTGTAATGCTTATATATAGAGAAGAGTATTATTTATCTAGATCAGAACCAGATCCAGGTACTCGAGA
ACACATAGAATGGAAAGTTAGACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACCGTAATGGGCCAG
TTGGTACAGTAAACTTACACTATGATAATAGGTATTCTAAATTTGGCAATATTGTTAAAAACTCTCAGCAAGGTTAA

Upstream 100 bases:

>100_bases
AAACAACTGTATGCAAGGTCTTGAATATGCTTTTAAAGCTCAAGGATTTTCTTTTGAGTTAGTTAAGTTCGAATAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TAAACGCTAGATTATGCATAAGATGAAAAAATGCTTGTCCAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAGAG
ATATGCAACTCAAGATTGGT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FIKLQTSIEESWTSISAAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSTLFNGKIGEQDVDKLKTVQNKIQKWNFFIDDAPAISISAIRSRARRL
KRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNIPVIALSQLSRAVEQRLDKKPILSDLRESGSIE
QDADIVMLIYREEYYLSRSEPDPGTREHIEWKVRQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG

Sequences:

>Translated_478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FIKLQTSIEESWTSISAAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSTLFNGKIGEQDVDKLKTVQNKIQKWNFFIDDAPAISISAIRSRARRL
KRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNIPVIALSQLSRAVEQRLDKKPILSDLRESGSIE
QDADIVMLIYREEYYLSRSEPDPGTREHIEWKVRQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG
>Mature_478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKG
FIKLQTSIEESWTSISAAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSTLFNGKIGEQDVDKLKTVQNKIQKWNFFIDDAPAISISAIRSRARRL
KRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNIPVIALSQLSRAVEQRLDKKPILSDLRESGSIE
QDADIVMLIYREEYYLSRSEPDPGTREHIEWKVRQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=35.8811040339703, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53414; Mature: 53414

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFIKLQTSIEESWTSISAAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHC
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCC
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSTLFNGKIGEQDVDKLKTVQNKIQKWNF
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCE
FIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLK
EECCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
ALAKELNIPVIALSQLSRAVEQRLDKKPILSDLRESGSIEQDADIVMLIYREEYYLSRSE
HHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECHHEECCCC
PDPGTREHIEWKVRQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG
CCCCCHHHEEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAITQIETAESQLYDLGSRGTLSKGFIKLQTSIEESWTSISAAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHC
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCC
DNVVPSVGFFSLEMSSQQISTRILSIESEINSSTLFNGKIGEQDVDKLKTVQNKIQKWNF
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCE
FIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLK
EECCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
ALAKELNIPVIALSQLSRAVEQRLDKKPILSDLRESGSIEQDADIVMLIYREEYYLSRSE
HHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECHHEECCCC
PDPGTREHIEWKVRQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQQG
CCCCCHHHEEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA