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The map label for this gene is pdhD [H]

Identifier: 189184453

GI number: 189184453

Start: 1576420

End: 1577847

Strand: Reverse

Name: pdhD [H]

Synonym: OTT_1546

Alternate gene names: 189184453

Gene position: 1577847-1576420 (Counterclockwise)

Preceding gene: 189184454

Following gene: 189184452

Centisome position: 78.54

GC content: 33.75

Gene sequence:

>1428_bases
ATGCCAGAAAGTTTTGATTTAGTAGTGATTGGCGGAGGACCAGGAGGATACACAGGAGCAATTAGAGGAGCTCAATTAGG
CATGAAGGTTGCATGTGTTGATAATAGGAATACTTTAGGAGGAACTTGTTTGAATGTAGGATGTATTCCTTCTAAATTTC
TGCTTCATGTGTCTAAAAAATATGAAGATATTAAAGCAGGTTTTGATGATTTAGGCATCAATGTAGGTGCAACAAAATTA
AATCTAGCTAATATGTTGAATAGTAAAAACAAAAGAGTACAAGAGTTAGGAAATGGCATTAGTGGATTGTTTAAAAAGAA
TAAAGTTAGTCATTTTGTTGGTACTGGAAAAATTATTAACAGTCATGAAATTTCTGTTCAAACTAGCTCTGGATCTAATA
CTAGCATTACTAATATTCATGCTAAAAACATATTGATTGCAACCGGATCAGAAATTACTACTTTGCCAGGTATTGAAATT
GATGAAGAATATATAGTTTCTTCTACTGGAGCTCTTAGTATTAAATCTGTTCCTAAAAAAATGTTAGTAATTGGTGGAGG
ATATATTGGCTTAGAGCTAGGTTCAGTATGGCGCAGGCTAGGAGCAGATGTGACAGTAGTTGAGCGTAGTGATAGAATAG
CTTCAGCAATGGACAATGAACTTGGAAAACTAACACAACAAGCACTATCAAAACAGGGCATTAAATTTAAATTTAACACT
AAAATTATATCAGCTGTAGTGAAAAATCAAACAGTTGATGTTATACTACAATCAGTTAATGGTGATAATATACAAGAAGA
AAAGATTAATGTTGATGTAGTATTAATCTCAGTCGGTAGAAAACCTTATGTTAAGAATTTAGGTTTAGAAAATATTGGTA
TAAAGCTTGACAAACAGCATGGTACTATTGAAGTAGATGAGAAATTTACAACTAATATAAAGAATATTTATGCAATTGGT
GATGTCATTAAAGGGCCAATGTTAGCGCATAAAGCTGAAGAAGAAGCTATAGCAGCAGTAGAAAATATGACTGGTCAGGC
TGGGCATGTTAATTATAATGTTATGCCAAGTGTTATTTATACTTTCCCAGAAGTTGCATCTGTAGGATATACTGAGGAGC
AGCTTAAAGAGCTTGGTATTCAATATGTTGTTGGAAAATTTCCATTTTTAGCTAATAGTAGAGCTAAAGTTATATATAAT
GATGTTACTGGAATGGTTAAAATTTTAGCTCATGCTAAGACTGATTTAATACTAGGAGTTCATATTATTGGCCCTGAAGC
TGGTACTTTAATTGCTGAAGCTGCTGTAGCGATGGAATTTCATGCTTCTGCAGAAGATATAGCTCGTACTTGCCATGCTC
ATCCTACTCTTAGCGAAGCATTGAAAGAAGCTGCGTTAGCAGTAGCTAAGAGAAGCGTTAATTATTAG

Upstream 100 bases:

>100_bases
GATCTTTAATGCTATATTTATACTAGACTTTTTATATTACTATCGGTAATTTAAGGTACTGTTGATATTGATTTGTTTTT
ATTAAATAAAAGAGGAAAAA

Downstream 100 bases:

>100_bases
ACTTTCTGCAAAATATTAAAAATAATTAGAGTTATGATTTAAGAAGTTAAAGCTGTAAAGTACATATCATTAAGCCTTGT
ATGTTGCAAACATGTGAAGT

Product: dihydrolipoamide dehydrogenase precursor

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPESFDLVVIGGGPGGYTGAIRGAQLGMKVACVDNRNTLGGTCLNVGCIPSKFLLHVSKKYEDIKAGFDDLGINVGATKL
NLANMLNSKNKRVQELGNGISGLFKKNKVSHFVGTGKIINSHEISVQTSSGSNTSITNIHAKNILIATGSEITTLPGIEI
DEEYIVSSTGALSIKSVPKKMLVIGGGYIGLELGSVWRRLGADVTVVERSDRIASAMDNELGKLTQQALSKQGIKFKFNT
KIISAVVKNQTVDVILQSVNGDNIQEEKINVDVVLISVGRKPYVKNLGLENIGIKLDKQHGTIEVDEKFTTNIKNIYAIG
DVIKGPMLAHKAEEEAIAAVENMTGQAGHVNYNVMPSVIYTFPEVASVGYTEEQLKELGIQYVVGKFPFLANSRAKVIYN
DVTGMVKILAHAKTDLILGVHIIGPEAGTLIAEAAVAMEFHASAEDIARTCHAHPTLSEALKEAALAVAKRSVNY

Sequences:

>Translated_475_residues
MPESFDLVVIGGGPGGYTGAIRGAQLGMKVACVDNRNTLGGTCLNVGCIPSKFLLHVSKKYEDIKAGFDDLGINVGATKL
NLANMLNSKNKRVQELGNGISGLFKKNKVSHFVGTGKIINSHEISVQTSSGSNTSITNIHAKNILIATGSEITTLPGIEI
DEEYIVSSTGALSIKSVPKKMLVIGGGYIGLELGSVWRRLGADVTVVERSDRIASAMDNELGKLTQQALSKQGIKFKFNT
KIISAVVKNQTVDVILQSVNGDNIQEEKINVDVVLISVGRKPYVKNLGLENIGIKLDKQHGTIEVDEKFTTNIKNIYAIG
DVIKGPMLAHKAEEEAIAAVENMTGQAGHVNYNVMPSVIYTFPEVASVGYTEEQLKELGIQYVVGKFPFLANSRAKVIYN
DVTGMVKILAHAKTDLILGVHIIGPEAGTLIAEAAVAMEFHASAEDIARTCHAHPTLSEALKEAALAVAKRSVNY
>Mature_474_residues
PESFDLVVIGGGPGGYTGAIRGAQLGMKVACVDNRNTLGGTCLNVGCIPSKFLLHVSKKYEDIKAGFDDLGINVGATKLN
LANMLNSKNKRVQELGNGISGLFKKNKVSHFVGTGKIINSHEISVQTSSGSNTSITNIHAKNILIATGSEITTLPGIEID
EEYIVSSTGALSIKSVPKKMLVIGGGYIGLELGSVWRRLGADVTVVERSDRIASAMDNELGKLTQQALSKQGIKFKFNTK
IISAVVKNQTVDVILQSVNGDNIQEEKINVDVVLISVGRKPYVKNLGLENIGIKLDKQHGTIEVDEKFTTNIKNIYAIGD
VIKGPMLAHKAEEEAIAAVENMTGQAGHVNYNVMPSVIYTFPEVASVGYTEEQLKELGIQYVVGKFPFLANSRAKVIYND
VTGMVKILAHAKTDLILGVHIIGPEAGTLIAEAAVAMEFHASAEDIARTCHAHPTLSEALKEAALAVAKRSVNY

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=54.8117154811716, Blast_Score=492, Evalue=1e-139,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI50301238, Length=472, Percent_Identity=27.1186440677966, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI22035672, Length=469, Percent_Identity=28.1449893390192, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI291045266, Length=447, Percent_Identity=27.2930648769575, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI291045268, Length=440, Percent_Identity=26.5909090909091, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=39.4335511982571, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=29.6218487394958, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=28.2511210762332, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI87081717, Length=472, Percent_Identity=27.7542372881356, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=54.2116630669546, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=27.7327935222672, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=25.5623721881391, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=456, Percent_Identity=26.7543859649123, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=456, Percent_Identity=26.7543859649123, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=261, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=52.3012552301255, Blast_Score=468, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=35.2818371607516, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6325166, Length=480, Percent_Identity=28.75, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=54.3340380549683, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=30.0613496932515, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=30.0613496932515, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=30.0613496932515, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17737741, Length=497, Percent_Identity=27.7665995975855, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50807; Mature: 50676

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESFDLVVIGGGPGGYTGAIRGAQLGMKVACVDNRNTLGGTCLNVGCIPSKFLLHVSKK
CCCCEEEEEEECCCCCCCCCCCHHCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHH
YEDIKAGFDDLGINVGATKLNLANMLNSKNKRVQELGNGISGLFKKNKVSHFVGTGKIIN
HHHHHCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCHHHEECCCEEEE
SHEISVQTSSGSNTSITNIHAKNILIATGSEITTLPGIEIDEEYIVSSTGALSIKSVPKK
CEEEEEEECCCCCCEEEEEEECEEEEECCCCEEECCCCCCCHHHEEECCCCEEECCCCCE
MLVIGGGYIGLELGSVWRRLGADVTVVERSDRIASAMDNELGKLTQQALSKQGIKFKFNT
EEEEECCEEEEHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEECH
KIISAVVKNQTVDVILQSVNGDNIQEEKINVDVVLISVGRKPYVKNLGLENIGIKLDKQH
HHHHHHHCCCEEEHHEECCCCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCCCEEEECCC
GTIEVDEKFTTNIKNIYAIGDVIKGPMLAHKAEEEAIAAVENMTGQAGHVNYNVMPSVIY
CEEEECCHHHCCHHHHEEHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHH
TFPEVASVGYTEEQLKELGIQYVVGKFPFLANSRAKVIYNDVTGMVKILAHAKTDLILGV
HCHHHHHCCCCHHHHHHCCHHEEECCCCEECCCCCEEEEHHHHHHHHHHHHCCCCEEEEE
HIIGPEAGTLIAEAAVAMEFHASAEDIARTCHAHPTLSEALKEAALAVAKRSVNY
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PESFDLVVIGGGPGGYTGAIRGAQLGMKVACVDNRNTLGGTCLNVGCIPSKFLLHVSKK
CCCEEEEEEECCCCCCCCCCCHHCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHH
YEDIKAGFDDLGINVGATKLNLANMLNSKNKRVQELGNGISGLFKKNKVSHFVGTGKIIN
HHHHHCCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCHHHEECCCEEEE
SHEISVQTSSGSNTSITNIHAKNILIATGSEITTLPGIEIDEEYIVSSTGALSIKSVPKK
CEEEEEEECCCCCCEEEEEEECEEEEECCCCEEECCCCCCCHHHEEECCCCEEECCCCCE
MLVIGGGYIGLELGSVWRRLGADVTVVERSDRIASAMDNELGKLTQQALSKQGIKFKFNT
EEEEECCEEEEHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEECH
KIISAVVKNQTVDVILQSVNGDNIQEEKINVDVVLISVGRKPYVKNLGLENIGIKLDKQH
HHHHHHHCCCEEEHHEECCCCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCCCEEEECCC
GTIEVDEKFTTNIKNIYAIGDVIKGPMLAHKAEEEAIAAVENMTGQAGHVNYNVMPSVIY
CEEEECCHHHCCHHHHEEHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHH
TFPEVASVGYTEEQLKELGIQYVVGKFPFLANSRAKVIYNDVTGMVKILAHAKTDLILGV
HCHHHHHCCCCHHHHHHCCHHEEECCCCEECCCCCEEEEHHHHHHHHHHHHCCCCEEEEE
HIIGPEAGTLIAEAAVAMEFHASAEDIARTCHAHPTLSEALKEAALAVAKRSVNY
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]