Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 189184452

GI number: 189184452

Start: 1574725

End: 1576086

Strand: Reverse

Name: tolB [H]

Synonym: OTT_1545

Alternate gene names: 189184452

Gene position: 1576086-1574725 (Counterclockwise)

Preceding gene: 189184453

Following gene: 189184451

Centisome position: 78.45

GC content: 29.74

Gene sequence:

>1362_bases
ATGATCAATAATTTATCCATCTCTATGACTAAAGTCATAAAAATTATATTAGCAATTATTATTATCTTATTTAACACTTT
ATCTATTTTTGCTAATACTACTACGTTATCTCCAATTAAAATTACTAAAGGTAACTGTACACAAATTCCAATAGCAATTA
ACTTTTTTGCTGCGAAGTCAAATGAAGAGCATGGCTTATCTCAAAATATTGTATCGATTATTAGTAATGATCTTAATATA
TCTAAAATTTTTGCTCCTATATCCAGTGATTTATTTATAGAAACTGAACAAGGCGTTGCTCATATACCATTATTTACTGC
TTGGAGTCAAATTAATGCTAATATTCTAATTAATGGAGAAATAACAAAAATAGATTCTACAGATTTCAAAGTTACTTTTG
TTGTATGGGATGTTTTCTCAGCTAAAGAAATTACTAGAAAATCTTTCACATTCCCTTCGCAACTATGGAGAAGTACAGCT
CATAAAATAGCAGATCAAATATATAAACATGTTACTGGAAGCAAGGGAAATTTTAATACAAAAATAGTATATGTTTCTGA
AAGCAATTCTTCTAAAGGAAAAATTAGGAGAATAGCTATAATGGATCAAGATGGAGCTAATCATAACTATATAACTAATG
GTAAAAATCACGTTATTACTCCTGTTTTTTCACCTAATAATAATCAAATACTTTATGTATCTTATCACAATAAAATACCA
ACAGTACGTATACATGACCTTAATTCTGGAAACAATAAAATATTAGCTAGTTTTAATGGTATCACGTTTTCGCCTAGATT
TTCTCCTGATGGTAATAAAATCCTAGTATCTAATTCATCAACCAAAAATGTTACACATATATATGAAATTAATTTACTTA
CTGGAAAAATAAAGCAATTAACTAAGGGGCAATCAATAAATACTTCACCTAGCTATTCTCCTGATGGCAGCAAAATTGCT
TTCGTGTCAGATCGTAGTGGATCAACACAAATATATATTATGAATGATCAAGGTGGTAATATCAAACGCTTAACTTCTCA
ACCTGGAGCATATACAACGCCAGCTTGGTCACCAACAAATAATTACATAGCATTTACTAAAATTGAAGCAGGAGAATTTA
GCATCGGAGTAATAAAATTAGATGGTAGTAATAAAAGAATAATTGCTACAAAACACTTAGTAGAAGGACCATCATGGGCT
CCAGATGGTAAAACTATCATTTTTTCACGTGCTTACAAAGCTACTAAATCAACTTCTACTAAAGTAAAATTATATTCTGT
AGACTGCACAGGATACAATGAGCGTGAAATTCAAACCCCTGAAAATGCTTCAGATCCTAACTGGTCAAATGAATATGAGT
AA

Upstream 100 bases:

>100_bases
AGGCTAAAAGTCTTGTATTTACAAGGTTACTTTTGAGTATACTATGGCTATGTTAAATTATTAAGTTTATTGTAAAATTG
TATAAATTTAGTATAGTAGA

Downstream 100 bases:

>100_bases
TATATCTGTATTGTAAAATTAAAATTAGTAGTGCATAGAAGCAAGTATATGTAAATTATTTTACTGATTGATATCACATA
ATTATTTAGTTTTATTGCAG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MINNLSISMTKVIKIILAIIIILFNTLSIFANTTTLSPIKITKGNCTQIPIAINFFAAKSNEEHGLSQNIVSIISNDLNI
SKIFAPISSDLFIETEQGVAHIPLFTAWSQINANILINGEITKIDSTDFKVTFVVWDVFSAKEITRKSFTFPSQLWRSTA
HKIADQIYKHVTGSKGNFNTKIVYVSESNSSKGKIRRIAIMDQDGANHNYITNGKNHVITPVFSPNNNQILYVSYHNKIP
TVRIHDLNSGNNKILASFNGITFSPRFSPDGNKILVSNSSTKNVTHIYEINLLTGKIKQLTKGQSINTSPSYSPDGSKIA
FVSDRSGSTQIYIMNDQGGNIKRLTSQPGAYTTPAWSPTNNYIAFTKIEAGEFSIGVIKLDGSNKRIIATKHLVEGPSWA
PDGKTIIFSRAYKATKSTSTKVKLYSVDCTGYNEREIQTPENASDPNWSNEYE

Sequences:

>Translated_453_residues
MINNLSISMTKVIKIILAIIIILFNTLSIFANTTTLSPIKITKGNCTQIPIAINFFAAKSNEEHGLSQNIVSIISNDLNI
SKIFAPISSDLFIETEQGVAHIPLFTAWSQINANILINGEITKIDSTDFKVTFVVWDVFSAKEITRKSFTFPSQLWRSTA
HKIADQIYKHVTGSKGNFNTKIVYVSESNSSKGKIRRIAIMDQDGANHNYITNGKNHVITPVFSPNNNQILYVSYHNKIP
TVRIHDLNSGNNKILASFNGITFSPRFSPDGNKILVSNSSTKNVTHIYEINLLTGKIKQLTKGQSINTSPSYSPDGSKIA
FVSDRSGSTQIYIMNDQGGNIKRLTSQPGAYTTPAWSPTNNYIAFTKIEAGEFSIGVIKLDGSNKRIIATKHLVEGPSWA
PDGKTIIFSRAYKATKSTSTKVKLYSVDCTGYNEREIQTPENASDPNWSNEYE
>Mature_453_residues
MINNLSISMTKVIKIILAIIIILFNTLSIFANTTTLSPIKITKGNCTQIPIAINFFAAKSNEEHGLSQNIVSIISNDLNI
SKIFAPISSDLFIETEQGVAHIPLFTAWSQINANILINGEITKIDSTDFKVTFVVWDVFSAKEITRKSFTFPSQLWRSTA
HKIADQIYKHVTGSKGNFNTKIVYVSESNSSKGKIRRIAIMDQDGANHNYITNGKNHVITPVFSPNNNQILYVSYHNKIP
TVRIHDLNSGNNKILASFNGITFSPRFSPDGNKILVSNSSTKNVTHIYEINLLTGKIKQLTKGQSINTSPSYSPDGSKIA
FVSDRSGSTQIYIMNDQGGNIKRLTSQPGAYTTPAWSPTNNYIAFTKIEAGEFSIGVIKLDGSNKRIIATKHLVEGPSWA
PDGKTIIFSRAYKATKSTSTKVKLYSVDCTGYNEREIQTPENASDPNWSNEYE

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=443, Percent_Identity=25.0564334085779, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 50132; Mature: 50132

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNLSISMTKVIKIILAIIIILFNTLSIFANTTTLSPIKITKGNCTQIPIAINFFAAKS
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCEEEEEEEEEEEECC
NEEHGLSQNIVSIISNDLNISKIFAPISSDLFIETEQGVAHIPLFTAWSQINANILINGE
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEEECEEEEEHHHCCCEEEECCE
ITKIDSTDFKVTFVVWDVFSAKEITRKSFTFPSQLWRSTAHKIADQIYKHVTGSKGNFNT
EEEECCCCEEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
KIVYVSESNSSKGKIRRIAIMDQDGANHNYITNGKNHVITPVFSPNNNQILYVSYHNKIP
EEEEEECCCCCCCEEEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCEEEEEEECCCCC
TVRIHDLNSGNNKILASFNGITFSPRFSPDGNKILVSNSSTKNVTHIYEINLLTGKIKQL
EEEEEECCCCCCEEEEEECCEEECCCCCCCCCEEEEECCCCCCEEEEEEEEEEEHHHHHH
TKGQSINTSPSYSPDGSKIAFVSDRSGSTQIYIMNDQGGNIKRLTSQPGAYTTPAWSPTN
HCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
NYIAFTKIEAGEFSIGVIKLDGSNKRIIATKHLVEGPSWAPDGKTIIFSRAYKATKSTST
CEEEEEEEECCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
KVKLYSVDCTGYNEREIQTPENASDPNWSNEYE
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINNLSISMTKVIKIILAIIIILFNTLSIFANTTTLSPIKITKGNCTQIPIAINFFAAKS
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCEEEEEEEEEEEECC
NEEHGLSQNIVSIISNDLNISKIFAPISSDLFIETEQGVAHIPLFTAWSQINANILINGE
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEEECEEEEEHHHCCCEEEECCE
ITKIDSTDFKVTFVVWDVFSAKEITRKSFTFPSQLWRSTAHKIADQIYKHVTGSKGNFNT
EEEECCCCEEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
KIVYVSESNSSKGKIRRIAIMDQDGANHNYITNGKNHVITPVFSPNNNQILYVSYHNKIP
EEEEEECCCCCCCEEEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCEEEEEEECCCCC
TVRIHDLNSGNNKILASFNGITFSPRFSPDGNKILVSNSSTKNVTHIYEINLLTGKIKQL
EEEEEECCCCCCEEEEEECCEEECCCCCCCCCEEEEECCCCCCEEEEEEEEEEEHHHHHH
TKGQSINTSPSYSPDGSKIAFVSDRSGSTQIYIMNDQGGNIKRLTSQPGAYTTPAWSPTN
HCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
NYIAFTKIEAGEFSIGVIKLDGSNKRIIATKHLVEGPSWAPDGKTIIFSRAYKATKSTST
CEEEEEEEECCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
KVKLYSVDCTGYNEREIQTPENASDPNWSNEYE
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA