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The map label for this gene is pheS [H]

Identifier: 189183503

GI number: 189183503

Start: 612352

End: 613386

Strand: Direct

Name: pheS [H]

Synonym: OTT_0596

Alternate gene names: 189183503

Gene position: 612352-613386 (Clockwise)

Preceding gene: 189183500

Following gene: 189183504

Centisome position: 30.48

GC content: 30.43

Gene sequence:

>1035_bases
ATGCTTAATCAAATTTTAGCTAAATTTAAAGAAGAAATTAGCAATATTAATGAAAAGAAGGCATTAAATGATCTTAAATT
ATCATATTTAGGAAAGTCAGGCATTATTACATTACAATTTAAAAAGATAGTAGAACTGAATGTTGAAAACAGAAAGGAGT
ATAGTAGTAGGGTCAATTTAGTTAAAAATGAGATTGAAACATTAATTGCTGCTAAGCTAGCTAAAATTTACAAGCAAGAA
ATGACTGCATTACTTGATAATGAGAAAGTTGATGTAACTTTGCCCTCAAGAAAAAAATTTTATGGTAGTATTCATCCAAT
TTCTCAGGTTACTGAAGAACTATTAAGTATATTTTCTATGTTTAATCTTACAGCTGTCGAAGGCCCAAATATAGAAAATG
ATTGGTATAATTTCAGCGCATTAAATATTGGTAGTAACCATGCAGCAAGACAGATGCATGATACCTTTTATTTAAATAAC
AGTGATACTAGCTTATTAAGGACTCACACTTCTACTATTCAAATACGAGCTATGGAACAGCAAAAACCTCCAATCTATCT
TGTTGCTCCAGGGCGTGTTTATCGTTCAGATTTTGACTCAACTCATACACCAATGTTTCATCAAATAGAGGGGTTAATAG
TAGATAAAAATATTCATATGGGACATTTAAAACATATAATTATTAATTTCATAAAAATGTTTTTTGAGCGGCAAGATATT
GAAATAATGTTTAGACCAAGCTTTTTCCCATTTACTGAGCCTTCAGCTGAGGTAGATATTAAATGCTGGATCAACAATAA
ATGGTTGGAAGTATTAGGATGTGGTATAGTACATCCTAATGTTTTACGTAATGTTAATATTGATCCAGACGAATATCAAG
GATTAGCTTTTGGATTAGGAGTTGAACGATTAGCAATGTTAAAATACGGTATTACTGACTTAAGAAAATTCTTTGAAAAT
GATCAGAGGTGGTTACAGCATTATAGCTTTTCAGCATTTAATATACCTACAATATTACATGGTCTAAGCAGATGA

Upstream 100 bases:

>100_bases
TACACTTAGTATCGCACTCAGAAAAGTTTCCGAATTACGTTGACAATCAAAATTAGTTATACTAATTTTTGTATCAAATT
TAATGATTTTATAATACTCA

Downstream 100 bases:

>100_bases
AGTTTACACCAAAATGGTTAAAACAGTTTTTAGTTACTGATGCAAACTTAATCGATATTACTCAAGCATTAACAGATCTT
GGTCTAGAAGTTGAATCAAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLNQILAKFKEEISNINEKKALNDLKLSYLGKSGIITLQFKKIVELNVENRKEYSSRVNLVKNEIETLIAAKLAKIYKQE
MTALLDNEKVDVTLPSRKKFYGSIHPISQVTEELLSIFSMFNLTAVEGPNIENDWYNFSALNIGSNHAARQMHDTFYLNN
SDTSLLRTHTSTIQIRAMEQQKPPIYLVAPGRVYRSDFDSTHTPMFHQIEGLIVDKNIHMGHLKHIIINFIKMFFERQDI
EIMFRPSFFPFTEPSAEVDIKCWINNKWLEVLGCGIVHPNVLRNVNIDPDEYQGLAFGLGVERLAMLKYGITDLRKFFEN
DQRWLQHYSFSAFNIPTILHGLSR

Sequences:

>Translated_344_residues
MLNQILAKFKEEISNINEKKALNDLKLSYLGKSGIITLQFKKIVELNVENRKEYSSRVNLVKNEIETLIAAKLAKIYKQE
MTALLDNEKVDVTLPSRKKFYGSIHPISQVTEELLSIFSMFNLTAVEGPNIENDWYNFSALNIGSNHAARQMHDTFYLNN
SDTSLLRTHTSTIQIRAMEQQKPPIYLVAPGRVYRSDFDSTHTPMFHQIEGLIVDKNIHMGHLKHIIINFIKMFFERQDI
EIMFRPSFFPFTEPSAEVDIKCWINNKWLEVLGCGIVHPNVLRNVNIDPDEYQGLAFGLGVERLAMLKYGITDLRKFFEN
DQRWLQHYSFSAFNIPTILHGLSR
>Mature_344_residues
MLNQILAKFKEEISNINEKKALNDLKLSYLGKSGIITLQFKKIVELNVENRKEYSSRVNLVKNEIETLIAAKLAKIYKQE
MTALLDNEKVDVTLPSRKKFYGSIHPISQVTEELLSIFSMFNLTAVEGPNIENDWYNFSALNIGSNHAARQMHDTFYLNN
SDTSLLRTHTSTIQIRAMEQQKPPIYLVAPGRVYRSDFDSTHTPMFHQIEGLIVDKNIHMGHLKHIIINFIKMFFERQDI
EIMFRPSFFPFTEPSAEVDIKCWINNKWLEVLGCGIVHPNVLRNVNIDPDEYQGLAFGLGVERLAMLKYGITDLRKFFEN
DQRWLQHYSFSAFNIPTILHGLSR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=261, Percent_Identity=31.0344827586207, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI5729820, Length=246, Percent_Identity=31.3008130081301, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=50.1529051987768, Blast_Score=350, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI17508957, Length=261, Percent_Identity=29.5019157088123, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563657, Length=261, Percent_Identity=29.5019157088123, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32566635, Length=252, Percent_Identity=31.7460317460317, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=349, Percent_Identity=28.3667621776504, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=272, Percent_Identity=24.2647058823529, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137424, Length=279, Percent_Identity=28.673835125448, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18858079, Length=269, Percent_Identity=29.368029739777, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39859; Mature: 39859

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQILAKFKEEISNINEKKALNDLKLSYLGKSGIITLQFKKIVELNVENRKEYSSRVNL
CHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCEEEEEEEEEEEECCCHHHHHHHHHHH
VKNEIETLIAAKLAKIYKQEMTALLDNEKVDVTLPSRKKFYGSIHPISQVTEELLSIFSM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHH
FNLTAVEGPNIENDWYNFSALNIGSNHAARQMHDTFYLNNSDTSLLRTHTSTIQIRAMEQ
HCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEECCEEEEEEECC
QKPPIYLVAPGRVYRSDFDSTHTPMFHQIEGLIVDKNIHMGHLKHIIINFIKMFFERQDI
CCCCEEEECCCHHHHCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCC
EIMFRPSFFPFTEPSAEVDIKCWINNKWLEVLGCGIVHPNVLRNVNIDPDEYQGLAFGLG
EEEECCCCCCCCCCCCCEEEEEEECCCCHHHHCCCCCCCHHHHCCCCCHHHHCCEEECCC
VERLAMLKYGITDLRKFFENDQRWLQHYSFSAFNIPTILHGLSR
HHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MLNQILAKFKEEISNINEKKALNDLKLSYLGKSGIITLQFKKIVELNVENRKEYSSRVNL
CHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCEEEEEEEEEEEECCCHHHHHHHHHHH
VKNEIETLIAAKLAKIYKQEMTALLDNEKVDVTLPSRKKFYGSIHPISQVTEELLSIFSM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHH
FNLTAVEGPNIENDWYNFSALNIGSNHAARQMHDTFYLNNSDTSLLRTHTSTIQIRAMEQ
HCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEECCEEEEEEECC
QKPPIYLVAPGRVYRSDFDSTHTPMFHQIEGLIVDKNIHMGHLKHIIINFIKMFFERQDI
CCCCEEEECCCHHHHCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCC
EIMFRPSFFPFTEPSAEVDIKCWINNKWLEVLGCGIVHPNVLRNVNIDPDEYQGLAFGLG
EEEECCCCCCCCCCCCCEEEEEEECCCCHHHHCCCCCCCHHHHCCCCCHHHHCCEEECCC
VERLAMLKYGITDLRKFFENDQRWLQHYSFSAFNIPTILHGLSR
HHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA