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The map label for this gene is pheT [H]

Identifier: 189183504

GI number: 189183504

Start: 613383

End: 615824

Strand: Direct

Name: pheT [H]

Synonym: OTT_0597

Alternate gene names: 189183504

Gene position: 613383-615824 (Clockwise)

Preceding gene: 189183503

Following gene: 189183511

Centisome position: 30.53

GC content: 30.18

Gene sequence:

>2442_bases
ATGAAGTTTACACCAAAATGGTTAAAACAGTTTTTAGTTACTGATGCAAACTTAATCGATATTACTCAAGCATTAACAGA
TCTTGGTCTAGAAGTTGAATCAATAATAGATCGTTCTGTTGAATTAAAAGATTTTATTATAGCTGAGATTATAGATATCA
AGCCTCATCCTAACGCACAAAAGTTGAAAATTTGCAAAGTTAATTCTGGAACAGAAATATTACAAATTGTCTGTGGAGCA
TCAAATGTAAGAGTAGGCCTTAAGGTAGTTCTTGCTGTACCTGGTACTAAAATTCCTAATGGTAATTTTGTAATCAAAGC
AACTAGCATTAGGGGGATTACAAGCGATGGAATGTTATGTTCAGAAGAAGAATTACTACTTAATAGTAATGATAATAATA
ATGGTATAATGGAATTACCACCTACTGCAACTGTAGGAGATTCATTTACAGCAGCTTTTGGATATGATGATCCAGTTATT
CAAATTAATGTTACACCAAATCGCGGAGATTGTTTAGGAATTTATGGCATTGCTAGAGAACTTCATGCTAAAGGATTAGG
TAAGCTACAATTAGTAGATGATAATTTTAAAACCTATACTAACGGACATTTTAAAAGCAATATAAAATTGAAGATATCTG
CTAAGGATGCTTGCTCATTATTTATTACTACTGAAATTCAAGATATTAATAATGCTTGTAGTACTCCATCTTGGTTGCAA
CATTTTTTACGTAATGTTGGTCTTAAATCAATTTCTCCTGTCGTAGATATTACTAATTATATTTGTTATAGTTTTGGTTA
CCCAATACATGCTTATGCTAAAGATAAGTTGTCAAACAATGAAATTGAAGTTAGAAAAGCTTATGCAAATGAAAGCATAA
TAGCGTTAAATCATCAAAGTTATAAATTAGATACTGCAGATTTAGTTGTAAGTTGTGGTAATAAGGTTATTGCATTAGCT
GGTATTATAGGAACATTAGATAGTGAATGTAAGTCTGAAACTAAGAATATAATCTTAGAAACTGGGTACTTTTTGGCAGA
TAGTATTATGACTACTGGTCGAAAATTGAATATTGAAAGCGATGCACGTTACCGCTTTGAAAGACAAGTTGATTATAATA
TGGCCAGTAAGGTTCATGCTATTGCCTTAAACTTAATTACTTCAATCTGCGGAGGCAAGATTTCAGAAACTGTCCTATAT
GATGATGCTACAACAACACCAGCTAAAACCTTAGAGTTTCCAATTAATGAATTTGAAAAGAAAACTGGAATTAGATTAAC
AAAGTCAACGATAATAAATATTTTAGGTAATTTGGGATTCACTATTTCCAACATTGCTGATGATACTTCTAGTATTTTGA
AACTTGAAATACCATCATGGCGTTCTGATATTGCAATTAAAGAAGATATAGTAGAAGAATTATTAAGAGTATATGGCTAT
AAATTTCTGCCTGAGGTACCAATTAAGAGGAATAACCAATCATTTACAGCATTACCTAGTAAGCAACAAAACATTTTAAC
AATCAAAAGAGTTGCTGCTGCATGTGGATATAATGAAGTGATCACTTGGTCATTTATGAATAGCAAAATAGTTAAATATT
TTAGTACTTATAATGAGGAATTAAAAATTTTAAATCCTATAAGTCAAGAGCTTGATTATATGCGGCCTACAATTATTCCT
AATTTACTTGAAGCAATAGCAAAAAATCAAGCAAGATCACTTAAAAATCAAGCTTTTTTTGAAATCGGACCAGTTTTTTC
TAAAACAGAGACATGCAATGAACAATGCATGCTTGCTGCTGTAAAATGCGGTAATAAGGAATTAATGCATCCGCATAGCT
CAATATCACAGTGGGATATTTTTGATATAAAAGCTGATTTAGAGGTTATACTACTTGAGTTAGGAATCAATATTGCAAAT
TGCAAAATTATTGGTACTCCTGCCCAGGAATATTACCATCCTGGTAGATTTGGTAGCATTATGCTAGGTAAAACTTGTAT
TGGATGTTTTGGTGAAATTCATCCTGAAATTTTATCTCAAGTTAATTTTTGTGGAATTAATATTACAAATAGAGTTATTG
CATTAGAGCTAAATATTGATTCGTTACCAATAAAAAAATCTCAATATGGTTATAAAAAACCAATTATGATATCTAATTAT
CAGCATATTGAACGAGATTTTGCATTTGTAATGGACAAATGTATACCAGCATCAGAAATAGTAACATCTATTAAGGAAGT
TAACAAACATTTAATTAAAACAGTTGAAGTATTTGATGTATATACCGGAGATAAAATAGATAATAATAAAAAATCCTTAG
CTCTTAGAGTTACAATACAATCTAATGATCAAACCTTAACTGAACCAATAATAAAAGAACTTAGCAACAATATAATATCA
AAGATAGAAAAGAAATTTAATGCGGTATTGAGAGATCATTAG

Upstream 100 bases:

>100_bases
TTACTGACTTAAGAAAATTCTTTGAAAATGATCAGAGGTGGTTACAGCATTATAGCTTTTCAGCATTTAATATACCTACA
ATATTACATGGTCTAAGCAG

Downstream 100 bases:

>100_bases
CTATTAAAAGTCGTATTCTCTACTATTTAACATAACAAGCTTTAACTCTTGCAAGGACTAAGAAATAAAATTTCTTAGTC
CTATTATTATTCAACTACTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MKFTPKWLKQFLVTDANLIDITQALTDLGLEVESIIDRSVELKDFIIAEIIDIKPHPNAQKLKICKVNSGTEILQIVCGA
SNVRVGLKVVLAVPGTKIPNGNFVIKATSIRGITSDGMLCSEEELLLNSNDNNNGIMELPPTATVGDSFTAAFGYDDPVI
QINVTPNRGDCLGIYGIARELHAKGLGKLQLVDDNFKTYTNGHFKSNIKLKISAKDACSLFITTEIQDINNACSTPSWLQ
HFLRNVGLKSISPVVDITNYICYSFGYPIHAYAKDKLSNNEIEVRKAYANESIIALNHQSYKLDTADLVVSCGNKVIALA
GIIGTLDSECKSETKNIILETGYFLADSIMTTGRKLNIESDARYRFERQVDYNMASKVHAIALNLITSICGGKISETVLY
DDATTTPAKTLEFPINEFEKKTGIRLTKSTIINILGNLGFTISNIADDTSSILKLEIPSWRSDIAIKEDIVEELLRVYGY
KFLPEVPIKRNNQSFTALPSKQQNILTIKRVAAACGYNEVITWSFMNSKIVKYFSTYNEELKILNPISQELDYMRPTIIP
NLLEAIAKNQARSLKNQAFFEIGPVFSKTETCNEQCMLAAVKCGNKELMHPHSSISQWDIFDIKADLEVILLELGINIAN
CKIIGTPAQEYYHPGRFGSIMLGKTCIGCFGEIHPEILSQVNFCGINITNRVIALELNIDSLPIKKSQYGYKKPIMISNY
QHIERDFAFVMDKCIPASEIVTSIKEVNKHLIKTVEVFDVYTGDKIDNNKKSLALRVTIQSNDQTLTEPIIKELSNNIIS
KIEKKFNAVLRDH

Sequences:

>Translated_813_residues
MKFTPKWLKQFLVTDANLIDITQALTDLGLEVESIIDRSVELKDFIIAEIIDIKPHPNAQKLKICKVNSGTEILQIVCGA
SNVRVGLKVVLAVPGTKIPNGNFVIKATSIRGITSDGMLCSEEELLLNSNDNNNGIMELPPTATVGDSFTAAFGYDDPVI
QINVTPNRGDCLGIYGIARELHAKGLGKLQLVDDNFKTYTNGHFKSNIKLKISAKDACSLFITTEIQDINNACSTPSWLQ
HFLRNVGLKSISPVVDITNYICYSFGYPIHAYAKDKLSNNEIEVRKAYANESIIALNHQSYKLDTADLVVSCGNKVIALA
GIIGTLDSECKSETKNIILETGYFLADSIMTTGRKLNIESDARYRFERQVDYNMASKVHAIALNLITSICGGKISETVLY
DDATTTPAKTLEFPINEFEKKTGIRLTKSTIINILGNLGFTISNIADDTSSILKLEIPSWRSDIAIKEDIVEELLRVYGY
KFLPEVPIKRNNQSFTALPSKQQNILTIKRVAAACGYNEVITWSFMNSKIVKYFSTYNEELKILNPISQELDYMRPTIIP
NLLEAIAKNQARSLKNQAFFEIGPVFSKTETCNEQCMLAAVKCGNKELMHPHSSISQWDIFDIKADLEVILLELGINIAN
CKIIGTPAQEYYHPGRFGSIMLGKTCIGCFGEIHPEILSQVNFCGINITNRVIALELNIDSLPIKKSQYGYKKPIMISNY
QHIERDFAFVMDKCIPASEIVTSIKEVNKHLIKTVEVFDVYTGDKIDNNKKSLALRVTIQSNDQTLTEPIIKELSNNIIS
KIEKKFNAVLRDH
>Mature_813_residues
MKFTPKWLKQFLVTDANLIDITQALTDLGLEVESIIDRSVELKDFIIAEIIDIKPHPNAQKLKICKVNSGTEILQIVCGA
SNVRVGLKVVLAVPGTKIPNGNFVIKATSIRGITSDGMLCSEEELLLNSNDNNNGIMELPPTATVGDSFTAAFGYDDPVI
QINVTPNRGDCLGIYGIARELHAKGLGKLQLVDDNFKTYTNGHFKSNIKLKISAKDACSLFITTEIQDINNACSTPSWLQ
HFLRNVGLKSISPVVDITNYICYSFGYPIHAYAKDKLSNNEIEVRKAYANESIIALNHQSYKLDTADLVVSCGNKVIALA
GIIGTLDSECKSETKNIILETGYFLADSIMTTGRKLNIESDARYRFERQVDYNMASKVHAIALNLITSICGGKISETVLY
DDATTTPAKTLEFPINEFEKKTGIRLTKSTIINILGNLGFTISNIADDTSSILKLEIPSWRSDIAIKEDIVEELLRVYGY
KFLPEVPIKRNNQSFTALPSKQQNILTIKRVAAACGYNEVITWSFMNSKIVKYFSTYNEELKILNPISQELDYMRPTIIP
NLLEAIAKNQARSLKNQAFFEIGPVFSKTETCNEQCMLAAVKCGNKELMHPHSSISQWDIFDIKADLEVILLELGINIAN
CKIIGTPAQEYYHPGRFGSIMLGKTCIGCFGEIHPEILSQVNFCGINITNRVIALELNIDSLPIKKSQYGYKKPIMISNY
QHIERDFAFVMDKCIPASEIVTSIKEVNKHLIKTVEVFDVYTGDKIDNNKKSLALRVTIQSNDQTLTEPIIKELSNNIIS
KIEKKFNAVLRDH

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=822, Percent_Identity=32.6034063260341, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR005728
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 90619; Mature: 90619

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTPKWLKQFLVTDANLIDITQALTDLGLEVESIIDRSVELKDFIIAEIIDIKPHPNAQ
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHEECCCCCCCC
KLKICKVNSGTEILQIVCGASNVRVGLKVVLAVPGTKIPNGNFVIKATSIRGITSDGMLC
EEEEEEECCCHHHHHEEECCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCEE
SEEELLLNSNDNNNGIMELPPTATVGDSFTAAFGYDDPVIQINVTPNRGDCLGIYGIARE
CCCCEEEECCCCCCCEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEHHHHHHH
LHAKGLGKLQLVDDNFKTYTNGHFKSNIKLKISAKDACSLFITTEIQDINNACSTPSWLQ
HHHCCCCEEEEECCCCCCCCCCEEECEEEEEEECCCCEEEEEEEEHHHHHCCCCCHHHHH
HFLRNVGLKSISPVVDITNYICYSFGYPIHAYAKDKLSNNEIEVRKAYANESIIALNHQS
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCC
YKLDTADLVVSCGNKVIALAGIIGTLDSECKSETKNIILETGYFLADSIMTTGRKLNIES
EECCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHCCCEEECCC
DARYRFERQVDYNMASKVHAIALNLITSICGGKISETVLYDDATTTPAKTLEFPINEFEK
CCCEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCHHHHHH
KTGIRLTKSTIINILGNLGFTISNIADDTSSILKLEIPSWRSDIAIKEDIVEELLRVYGY
HCCCEEHHHHHHHHHHHCCEEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
KFLPEVPIKRNNQSFTALPSKQQNILTIKRVAAACGYNEVITWSFMNSKIVKYFSTYNEE
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCC
LKILNPISQELDYMRPTIIPNLLEAIAKNQARSLKNQAFFEIGPVFSKTETCNEQCMLAA
CEEECHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHEEHE
VKCGNKELMHPHSSISQWDIFDIKADLEVILLELGINIANCKIIGTPAQEYYHPGRFGSI
ECCCCHHHCCCCCCCCCCEEEEECCCHHEEEEECCCCEEEEEEECCCHHHHCCCCCCCCE
MLGKTCIGCFGEIHPEILSQVNFCGINITNRVIALELNIDSLPIKKSQYGYKKPIMISNY
EHHHHHHHHHHHCCHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCEEECCH
QHIERDFAFVMDKCIPASEIVTSIKEVNKHLIKTVEVFDVYTGDKIDNNKKSLALRVTIQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEEEEE
SNDQTLTEPIIKELSNNIISKIEKKFNAVLRDH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFTPKWLKQFLVTDANLIDITQALTDLGLEVESIIDRSVELKDFIIAEIIDIKPHPNAQ
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHEECCCCCCCC
KLKICKVNSGTEILQIVCGASNVRVGLKVVLAVPGTKIPNGNFVIKATSIRGITSDGMLC
EEEEEEECCCHHHHHEEECCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCEE
SEEELLLNSNDNNNGIMELPPTATVGDSFTAAFGYDDPVIQINVTPNRGDCLGIYGIARE
CCCCEEEECCCCCCCEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEHHHHHHH
LHAKGLGKLQLVDDNFKTYTNGHFKSNIKLKISAKDACSLFITTEIQDINNACSTPSWLQ
HHHCCCCEEEEECCCCCCCCCCEEECEEEEEEECCCCEEEEEEEEHHHHHCCCCCHHHHH
HFLRNVGLKSISPVVDITNYICYSFGYPIHAYAKDKLSNNEIEVRKAYANESIIALNHQS
HHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCC
YKLDTADLVVSCGNKVIALAGIIGTLDSECKSETKNIILETGYFLADSIMTTGRKLNIES
EECCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHCCCEEECCC
DARYRFERQVDYNMASKVHAIALNLITSICGGKISETVLYDDATTTPAKTLEFPINEFEK
CCCEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCHHHHHH
KTGIRLTKSTIINILGNLGFTISNIADDTSSILKLEIPSWRSDIAIKEDIVEELLRVYGY
HCCCEEHHHHHHHHHHHCCEEEHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
KFLPEVPIKRNNQSFTALPSKQQNILTIKRVAAACGYNEVITWSFMNSKIVKYFSTYNEE
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCC
LKILNPISQELDYMRPTIIPNLLEAIAKNQARSLKNQAFFEIGPVFSKTETCNEQCMLAA
CEEECHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHEEHE
VKCGNKELMHPHSSISQWDIFDIKADLEVILLELGINIANCKIIGTPAQEYYHPGRFGSI
ECCCCHHHCCCCCCCCCCEEEEECCCHHEEEEECCCCEEEEEEECCCHHHHCCCCCCCCE
MLGKTCIGCFGEIHPEILSQVNFCGINITNRVIALELNIDSLPIKKSQYGYKKPIMISNY
EHHHHHHHHHHHCCHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCEEECCH
QHIERDFAFVMDKCIPASEIVTSIKEVNKHLIKTVEVFDVYTGDKIDNNKKSLALRVTIQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEEEEE
SNDQTLTEPIIKELSNNIISKIEKKFNAVLRDH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA