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The map label for this gene is bcr2 [H]

Identifier: 189183361

GI number: 189183361

Start: 475774

End: 476955

Strand: Direct

Name: bcr2 [H]

Synonym: OTT_0454

Alternate gene names: 189183361

Gene position: 475774-476955 (Clockwise)

Preceding gene: 189183350

Following gene: 189183363

Centisome position: 23.68

GC content: 29.44

Gene sequence:

>1182_bases
ATGATAAATAACCATTGGAAGTATAATTTAACGATAATCACTCTGCTAATATTTATTACAGAGTTAGCAACTGATATTTA
TGTTCCTAGCTTACCTAGAATAGCTAGCGCATTTAATACATCAGAGTATAAAGTAATGTTGACTGTAAGTATCAACCTAA
TAGGGTTATCATTATCTGGATTATTATATGGTCCATTATCTGATGCAATAGGGCGTAAGAAAGTTTTACTATTAGGAATA
ACAATTTTTTGTTTTTCGAGTTTATTTTGTATATACGTAAGCACGATTGAGTTAATAGTATTTTGGAGATTATTACAAGG
AATTGGAGGTGGAGCCTCAGTTACAGTTGGGTTAGCTACAATAAGCGATATATATAAAGGTAATGAAAGAGCAAAAATGA
TGTCAAGACTAAATATGGTAGTTGCTTTTTCTCCAGCACTTGGGCCTATAATTGGTAGTCAGATAGCAGCATATGATAAT
AGGTGGTATTTACCACTTGCTATCATTCCTTCTCTTGCATTTCCAGTACTTTTATTACTAATAACTTTATTCAAGGAAAC
TTTAACTCAGACAAATATAGGAATTAATTTTAATAATATTAAAGCTAGCTTTAAGGAAGTATTATCTAATAAAAATTTTA
TATGTTATTTATTGATTCAATCTTTAACATTTGGATGGTTATGGGCAGATATAGTAAACTTACCATTTATTTTTATTAAA
GATATGAATGTTCCAACAGAGCACTACGGATATTACATTATTGCAAATGTTACAGTCTATATTATAGGTATTGTAGCCAA
TCAAAAACTTGTCCAATTTAAAGGACCTGAAAAAATGATACTATATGGTATTGTATTAATACTATTCAGCGGTATATCAT
TAACTTTTGGTACAATCTATTTTACTCTGACAGCATATATAGTTCTACTTCTTAAGTTACCAGCTGCTATTGGAGTTGGA
TTTACTTTAGGTAATGCATCAGCATTATCTTTAATGCAAATTACAAACTATGGAACAGCCTCTGCAATCATAGGTTCTAC
TGAAATGCTTTTAGGATCCTTAGGTATAAAAATAATCAGTTATCTCTATAATGGTACGATATTACCTCTTTCGATATTAG
TAGTAGTATTTTCATTAATTTCTTTGCTTATTTTTCAGAAAATAAAATCTCCTACTGTTTAA

Upstream 100 bases:

>100_bases
ATGCTTATGTATTAAGTAGTTTAATTGTATTATTAATATGCTCATTTTTGCAGAAATCATGCTAAGTAAAGTTAAACAAT
AGTTTTATAATGTTGATACA

Downstream 100 bases:

>100_bases
TCGCAAAAAGAGAAACAATTTATAAACACCAAGGATAATGAGTTAGAAGAGAAAGAAGAGGAGATAAGATTGGAATTAAG
CTGGAGGAGATAGAATAAGT

Product: drug resistance transporter, Bcr/CflA family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MINNHWKYNLTIITLLIFITELATDIYVPSLPRIASAFNTSEYKVMLTVSINLIGLSLSGLLYGPLSDAIGRKKVLLLGI
TIFCFSSLFCIYVSTIELIVFWRLLQGIGGGASVTVGLATISDIYKGNERAKMMSRLNMVVAFSPALGPIIGSQIAAYDN
RWYLPLAIIPSLAFPVLLLLITLFKETLTQTNIGINFNNIKASFKEVLSNKNFICYLLIQSLTFGWLWADIVNLPFIFIK
DMNVPTEHYGYYIIANVTVYIIGIVANQKLVQFKGPEKMILYGIVLILFSGISLTFGTIYFTLTAYIVLLLKLPAAIGVG
FTLGNASALSLMQITNYGTASAIIGSTEMLLGSLGIKIISYLYNGTILPLSILVVVFSLISLLIFQKIKSPTV

Sequences:

>Translated_393_residues
MINNHWKYNLTIITLLIFITELATDIYVPSLPRIASAFNTSEYKVMLTVSINLIGLSLSGLLYGPLSDAIGRKKVLLLGI
TIFCFSSLFCIYVSTIELIVFWRLLQGIGGGASVTVGLATISDIYKGNERAKMMSRLNMVVAFSPALGPIIGSQIAAYDN
RWYLPLAIIPSLAFPVLLLLITLFKETLTQTNIGINFNNIKASFKEVLSNKNFICYLLIQSLTFGWLWADIVNLPFIFIK
DMNVPTEHYGYYIIANVTVYIIGIVANQKLVQFKGPEKMILYGIVLILFSGISLTFGTIYFTLTAYIVLLLKLPAAIGVG
FTLGNASALSLMQITNYGTASAIIGSTEMLLGSLGIKIISYLYNGTILPLSILVVVFSLISLLIFQKIKSPTV
>Mature_393_residues
MINNHWKYNLTIITLLIFITELATDIYVPSLPRIASAFNTSEYKVMLTVSINLIGLSLSGLLYGPLSDAIGRKKVLLLGI
TIFCFSSLFCIYVSTIELIVFWRLLQGIGGGASVTVGLATISDIYKGNERAKMMSRLNMVVAFSPALGPIIGSQIAAYDN
RWYLPLAIIPSLAFPVLLLLITLFKETLTQTNIGINFNNIKASFKEVLSNKNFICYLLIQSLTFGWLWADIVNLPFIFIK
DMNVPTEHYGYYIIANVTVYIIGIVANQKLVQFKGPEKMILYGIVLILFSGISLTFGTIYFTLTAYIVLLLKLPAAIGVG
FTLGNASALSLMQITNYGTASAIIGSTEMLLGSLGIKIISYLYNGTILPLSILVVVFSLISLLIFQKIKSPTV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=357, Percent_Identity=25.7703081232493, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790146, Length=360, Percent_Identity=25.5555555555556, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI48994889, Length=272, Percent_Identity=27.2058823529412, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790794, Length=380, Percent_Identity=25.7894736842105, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082312, Length=199, Percent_Identity=27.6381909547739, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787065, Length=359, Percent_Identity=25.3481894150418, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=159, Percent_Identity=27.0440251572327, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43211; Mature: 43211

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNHWKYNLTIITLLIFITELATDIYVPSLPRIASAFNTSEYKVMLTVSINLIGLSLSG
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEEEEEEEEEECC
LLYGPLSDAIGRKKVLLLGITIFCFSSLFCIYVSTIELIVFWRLLQGIGGGASVTVGLAT
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHH
ISDIYKGNERAKMMSRLNMVVAFSPALGPIIGSQIAAYDNRWYLPLAIIPSLAFPVLLLL
HHHHHCCCHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
ITLFKETLTQTNIGINFNNIKASFKEVLSNKNFICYLLIQSLTFGWLWADIVNLPFIFIK
HHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEE
DMNVPTEHYGYYIIANVTVYIIGIVANQKLVQFKGPEKMILYGIVLILFSGISLTFGTIY
CCCCCHHHCCEEEEEEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
FTLTAYIVLLLKLPAAIGVGFTLGNASALSLMQITNYGTASAIIGSTEMLLGSLGIKIIS
HHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHH
YLYNGTILPLSILVVVFSLISLLIFQKIKSPTV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MINNHWKYNLTIITLLIFITELATDIYVPSLPRIASAFNTSEYKVMLTVSINLIGLSLSG
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEEEEEEEEEECC
LLYGPLSDAIGRKKVLLLGITIFCFSSLFCIYVSTIELIVFWRLLQGIGGGASVTVGLAT
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHH
ISDIYKGNERAKMMSRLNMVVAFSPALGPIIGSQIAAYDNRWYLPLAIIPSLAFPVLLLL
HHHHHCCCHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
ITLFKETLTQTNIGINFNNIKASFKEVLSNKNFICYLLIQSLTFGWLWADIVNLPFIFIK
HHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEE
DMNVPTEHYGYYIIANVTVYIIGIVANQKLVQFKGPEKMILYGIVLILFSGISLTFGTIY
CCCCCHHHCCEEEEEEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
FTLTAYIVLLLKLPAAIGVGFTLGNASALSLMQITNYGTASAIIGSTEMLLGSLGIKIIS
HHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHH
YLYNGTILPLSILVVVFSLISLLIFQKIKSPTV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]